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The map label for this gene is leuD [H]

Identifier: 16763500

GI number: 16763500

Start: 129057

End: 129662

Strand: Reverse

Name: leuD [H]

Synonym: STM0110

Alternate gene names: 16763500

Gene position: 129662-129057 (Counterclockwise)

Preceding gene: 16763501

Following gene: 16763499

Centisome position: 2.67

GC content: 52.97

Gene sequence:

>606_bases
ATGGCAGAGAAATTTACCCAGCATACCGGCCTGGTTGTCCCACTGGATGCCGCCAACGTCGATACCGATGCAATTATCCC
TAAACAGTTTTTGCAGAAGGTTACGCGCACCGGTTTTGGCGCCCATCTGTTTAACGACTGGCGTTTCCTGGACGAAAAGG
GCCAACAGCCAAATCCGGAATTCGTGTTGAACTTTCCGGAATATCAAGGCGCGTCGATACTGTTGGCGCGGGAAAACTTT
GGCTGCGGCTCGTCACGCGAGCACGCGCCGTGGGCGTTGACCGATTACGGCTTTAAAGTGGTGATCGCGCCAAGCTTCGC
CGACATCTTCTACGGCAACAGTTTCAATAATCAACTGCTGCCGGTAACCCTGAGCGACGCACAGGTCGATGAGCTGTTTG
CCCTGGTGAAAGCCAATCCGGGCATTAAATTTGAAGTGGATCTGGAAGCACAGGTGGTGAAAGCAGGCGATAAAACCTAC
AGCTTTAAAATCGACGACTTCCGCCGCCACTGCATGTTGAACGGTCTGGACAGCATTGGGCTGACGCTGCAGCACGAAGA
CGCGATTGCCGCCTACGAAAATAAACAACCGGCATTTATGCGGTAA

Upstream 100 bases:

>100_bases
CGCGGGGGTCGCACGCATTTAGTCAGCCCGGCGATGGCCGCCGCTGCCGCCGTTACCGGCCACTTCGCCGACATTCGCAG
CATCAAATAAGGAAACTACC

Downstream 100 bases:

>100_bases
CCCTTGACGCCTTACCGGGCCTACAGGTTATGCAATATCACCCGGTTGCCATTATATTCTGTAGGCCTGATAAGCGTAGC
GCCATCAAGCTCCGGGTTAC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MAEKFTQHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLFNDWRFLDEKGQQPNPEFVLNFPEYQGASILLARENF
GCGSSREHAPWALTDYGFKVVIAPSFADIFYGNSFNNQLLPVTLSDAQVDELFALVKANPGIKFEVDLEAQVVKAGDKTY
SFKIDDFRRHCMLNGLDSIGLTLQHEDAIAAYENKQPAFMR

Sequences:

>Translated_201_residues
MAEKFTQHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLFNDWRFLDEKGQQPNPEFVLNFPEYQGASILLARENF
GCGSSREHAPWALTDYGFKVVIAPSFADIFYGNSFNNQLLPVTLSDAQVDELFALVKANPGIKFEVDLEAQVVKAGDKTY
SFKIDDFRRHCMLNGLDSIGLTLQHEDAIAAYENKQPAFMR
>Mature_200_residues
AEKFTQHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLFNDWRFLDEKGQQPNPEFVLNFPEYQGASILLARENFG
CGSSREHAPWALTDYGFKVVIAPSFADIFYGNSFNNQLLPVTLSDAQVDELFALVKANPGIKFEVDLEAQVVKAGDKTYS
FKIDDFRRHCMLNGLDSIGLTLQHEDAIAAYENKQPAFMR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=200, Percent_Identity=92, Blast_Score=386, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321429, Length=196, Percent_Identity=52.0408163265306, Blast_Score=191, Evalue=7e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22507; Mature: 22375

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKFTQHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLFNDWRFLDEKGQQPNPE
CCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCHHHHHCCCCCCCC
FVLNFPEYQGASILLARENFGCGSSREHAPWALTDYGFKVVIAPSFADIFYGNSFNNQLL
EEEECCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCHHEEEECCCCCCEEE
PVTLSDAQVDELFALVKANPGIKFEVDLEAQVVKAGDKTYSFKIDDFRRHCMLNGLDSIG
EEEECCCCHHHHHHHEECCCCCEEEECCCEEEEECCCCEEEEEEHHHHHHHHHCCCCCCC
LTLQHEDAIAAYENKQPAFMR
EEEECCCCEEEECCCCCCCCC
>Mature Secondary Structure 
AEKFTQHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLFNDWRFLDEKGQQPNPE
CCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCHHHHHCCCCCCCC
FVLNFPEYQGASILLARENFGCGSSREHAPWALTDYGFKVVIAPSFADIFYGNSFNNQLL
EEEECCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCHHEEEECCCCCCEEE
PVTLSDAQVDELFALVKANPGIKFEVDLEAQVVKAGDKTYSFKIDDFRRHCMLNGLDSIG
EEEECCCCHHHHHHHEECCCCCEEEECCCEEEEECCCCEEEEEEHHHHHHHHHCCCCCCC
LTLQHEDAIAAYENKQPAFMR
EEEECCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA