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The map label for this gene is citA [H]

Identifier: 16763443

GI number: 16763443

Start: 61794

End: 63413

Strand: Reverse

Name: citA [H]

Synonym: STM0053

Alternate gene names: 16763443

Gene position: 63413-61794 (Counterclockwise)

Preceding gene: 16763444

Following gene: 16763442

Centisome position: 1.31

GC content: 48.02

Gene sequence:

>1620_bases
ATGTTTATACGGGAAATTACACGCTGGTTTGCCAGACACTCGTTTCAAAACCGGATATTTCTACTGATCCTGTTTACATC
CACCATCGTGATGCTGGCAATCACCTGGTATCTGACCGATATCACGAAAGAACGCCTGCATTATCAGGTGGGCCAACGTG
CGCTTATTCAGGCAATGCAAATATCCGCTATGCCAGAATTAGTTGAAGCCGTAGAAGCGCATGATTTGTCACGCATCAAA
GCGTTGATTGACCCAATGCGCTCGTTTTCGGATGCGACTTACATTACCGTTGGCAACGAAAAAGGTCAGCGACTGTATCA
CGTCAACCCGGATGAGATCGGCAAATATATGGAGGGCGGCGATAGCGACGACGCGCTGTATAACGCTAAAAGCTACGTAT
CGGTGCGCAAAGGTTCATTAGGCTCTTCCCTACGTGGTAAATCCCCGATTCAGGACAGTACCGGTAAAGTCATCGGTATT
GTTTCCGTCGGCTATACTCTGGAACAGTTGGAGAGCTGGCTTAACTTACAAATCAGCTCGTTGCTGTTCCCGATGGCGTT
ACTGCTGCTGGTACTGTTGTACTGTGCCCGCCGCTTCTCGCTGCATATTAAAAAGCAGATGCTTAACATGGAGCCGCAGC
AGCTTTCACAACTATTAATTCAGCAAAGCGTGTTATTTGAGTCGGTATTTGAAGGGTTGATCGCCATTGACTCCCATCAC
CGTATTACCGCCATTAACCAAACGGCTCGCCGCTTACTGAATCTGAGCCAACCGGAGTCTGAACTCATTGGCTATGCAAT
CCACCATGTCGTGTTGCCGGAGGCCTTCTTTTACGATGCGCCGCAAAAAAACAAAAAAGATGAGATCGTCACCTTTAACC
AAATGAAAGTCATCGCCAGTCGCATGGCGGTCATTATTGACAACCAGCCTCAGGGCTGGGTTATTAGCTTTCGCAACAAA
GACGATATCAATACACTCAGCCTGCAGTTAAGTCAGGTGCGGCAATACGCCGATAACCTGCGCGCCGTGCAGCATGAGCA
TCGCAACCTTATTTCCACCATTGCCGGACTGCTGTTCCTGAAGCGCTACGATAATGCGCTGGCGCTGATTCAGCAGCAGT
CGGAAAGCCACCAGAAAGTACTCGATTTTATTTCCCATAATTTCCGCGACCACCACCTTGCGGGGCTATTAATCGGTAAA
TATTATCGGGCAAAAGAGCTGGGGCTGGAGCTGATTTTCGATCCTTCAAGCTTTGTTGAACACCTGCCGTCATCGCTCTC
GCATAATGAATGGATTTCGATTGTTGGCAATTTATTGGACAACGCCTATAACGCCACCCTGCTCAATCGCGAAGGCTCGC
GACAGATTGAGTGCCTGATCAACAGCGAAGGTAATGAAGTCATTATTGAAGTTGCCGATCAGGGATGTGGTATTGACGAA
AATATCCGCCAGCATATTTTCGAGCGTGGAGTGACCACCCACAACAACGGCGATCACGGAATTGGATTATGGTTGGTGCA
CAGCTACGTCAAGCAGGCCAATGGTGACATTATCGTCGATAACAATAATCCTTTTGGCACCATTTTTACCCTCTATATCC
CTTTTACCAGAGAAGAATAA

Upstream 100 bases:

>100_bases
CATCCAAATTTTTTCTGAAAGAAAAGTAAGTATGAACTGGTTCAGGTTCCGGTGATGCCGAATGCAAGAAAATACCGTAA
TTAAATATTTAAATACCAAT

Downstream 100 bases:

>100_bases
CATGGATAGCATTACGACACTCATCGTTGAAGACGAACCGATGCTAGCGGAAATCCTGGTGGACACCATCAAGATTTTTC
CCCAGTTTTCAATCGTCGGC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MFIREITRWFARHSFQNRIFLLILFTSTIVMLAITWYLTDITKERLHYQVGQRALIQAMQISAMPELVEAVEAHDLSRIK
ALIDPMRSFSDATYITVGNEKGQRLYHVNPDEIGKYMEGGDSDDALYNAKSYVSVRKGSLGSSLRGKSPIQDSTGKVIGI
VSVGYTLEQLESWLNLQISSLLFPMALLLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFESVFEGLIAIDSHH
RITAINQTARRLLNLSQPESELIGYAIHHVVLPEAFFYDAPQKNKKDEIVTFNQMKVIASRMAVIIDNQPQGWVISFRNK
DDINTLSLQLSQVRQYADNLRAVQHEHRNLISTIAGLLFLKRYDNALALIQQQSESHQKVLDFISHNFRDHHLAGLLIGK
YYRAKELGLELIFDPSSFVEHLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEVIIEVADQGCGIDE
NIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNPFGTIFTLYIPFTREE

Sequences:

>Translated_539_residues
MFIREITRWFARHSFQNRIFLLILFTSTIVMLAITWYLTDITKERLHYQVGQRALIQAMQISAMPELVEAVEAHDLSRIK
ALIDPMRSFSDATYITVGNEKGQRLYHVNPDEIGKYMEGGDSDDALYNAKSYVSVRKGSLGSSLRGKSPIQDSTGKVIGI
VSVGYTLEQLESWLNLQISSLLFPMALLLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFESVFEGLIAIDSHH
RITAINQTARRLLNLSQPESELIGYAIHHVVLPEAFFYDAPQKNKKDEIVTFNQMKVIASRMAVIIDNQPQGWVISFRNK
DDINTLSLQLSQVRQYADNLRAVQHEHRNLISTIAGLLFLKRYDNALALIQQQSESHQKVLDFISHNFRDHHLAGLLIGK
YYRAKELGLELIFDPSSFVEHLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEVIIEVADQGCGIDE
NIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNPFGTIFTLYIPFTREE
>Mature_539_residues
MFIREITRWFARHSFQNRIFLLILFTSTIVMLAITWYLTDITKERLHYQVGQRALIQAMQISAMPELVEAVEAHDLSRIK
ALIDPMRSFSDATYITVGNEKGQRLYHVNPDEIGKYMEGGDSDDALYNAKSYVSVRKGSLGSSLRGKSPIQDSTGKVIGI
VSVGYTLEQLESWLNLQISSLLFPMALLLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFESVFEGLIAIDSHH
RITAINQTARRLLNLSQPESELIGYAIHHVVLPEAFFYDAPQKNKKDEIVTFNQMKVIASRMAVIIDNQPQGWVISFRNK
DDINTLSLQLSQVRQYADNLRAVQHEHRNLISTIAGLLFLKRYDNALALIQQQSESHQKVLDFISHNFRDHHLAGLLIGK
YYRAKELGLELIFDPSSFVEHLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEVIIEVADQGCGIDE
NIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNPFGTIFTLYIPFTREE

Specific function: Member of the two-component regulatory system CitA/CitB. Probably activates CitB by phosphorylation. The periplasmic domain binds H-citrate(2-), which is essential for induction of the citrate-fermentation genes [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=532, Percent_Identity=42.2932330827068, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1790567, Length=556, Percent_Identity=28.5971223021583, Blast_Score=182, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61399; Mature: 61399

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIREITRWFARHSFQNRIFLLILFTSTIVMLAITWYLTDITKERLHYQVGQRALIQAMQ
CCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISAMPELVEAVEAHDLSRIKALIDPMRSFSDATYITVGNEKGQRLYHVNPDEIGKYMEGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCHHHHHHHHCCC
DSDDALYNAKSYVSVRKGSLGSSLRGKSPIQDSTGKVIGIVSVGYTLEQLESWLNLQISS
CCCCHHHCHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
LLFPMALLLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFESVFEGLIAIDSHH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCC
RITAINQTARRLLNLSQPESELIGYAIHHVVLPEAFFYDAPQKNKKDEIVTFNQMKVIAS
EEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHH
RMAVIIDNQPQGWVISFRNKDDINTLSLQLSQVRQYADNLRAVQHEHRNLISTIAGLLFL
HHEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRYDNALALIQQQSESHQKVLDFISHNFRDHHLAGLLIGKYYRAKELGLELIFDPSSFVE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEECCHHHHH
HLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEVIIEVADQGCGIDE
HCHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCEEEEEEECCCCCCCH
NIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNPFGTIFTLYIPFTREE
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEEECCCC
>Mature Secondary Structure
MFIREITRWFARHSFQNRIFLLILFTSTIVMLAITWYLTDITKERLHYQVGQRALIQAMQ
CCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISAMPELVEAVEAHDLSRIKALIDPMRSFSDATYITVGNEKGQRLYHVNPDEIGKYMEGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCHHHHHHHHCCC
DSDDALYNAKSYVSVRKGSLGSSLRGKSPIQDSTGKVIGIVSVGYTLEQLESWLNLQISS
CCCCHHHCHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
LLFPMALLLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFESVFEGLIAIDSHH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCC
RITAINQTARRLLNLSQPESELIGYAIHHVVLPEAFFYDAPQKNKKDEIVTFNQMKVIAS
EEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHH
RMAVIIDNQPQGWVISFRNKDDINTLSLQLSQVRQYADNLRAVQHEHRNLISTIAGLLFL
HHEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRYDNALALIQQQSESHQKVLDFISHNFRDHHLAGLLIGKYYRAKELGLELIFDPSSFVE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEECCHHHHH
HLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEVIIEVADQGCGIDE
HCHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCEEEEEEECCCCCCCH
NIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNPFGTIFTLYIPFTREE
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8748036; 10447894 [H]