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The map label for this gene is rpoS [H]

Identifier: 218930373

GI number: 218930373

Start: 3744225

End: 3745223

Strand: Reverse

Name: rpoS [H]

Synonym: YPO3355

Alternate gene names: 218930373

Gene position: 3745223-3744225 (Counterclockwise)

Preceding gene: 218930374

Following gene: 218930370

Centisome position: 80.48

GC content: 47.45

Gene sequence:

>999_bases
ATGAGCCAAAGTACGCTGAAAGTTAACGAGTTGCATGAAGATGCTGATTTTGATGAGAACAGTACGGAAACTGAGATTTT
TGATGAAAAAGCATTAGTAGATGATGAACCTACTGAAAGCGAGTTAGCAGACGATGAGTTGTTGGCGCAAGGTGTTACCC
AGCGTGTGCTGGATGCGACGCAACTCTATCTTGGTGAAATCGGTTATTCGCCACTGCTAACGGCCGAAGAAGAGGTTTAT
TTTGCCCGGCGTGCATTGCGCGGAGACGTATCTTCACGTCGACGTATGATTGAGAGTAACTTACGGCTGGTGGTGAAGAT
CGCCCGCCGTTACAGTAATCGCGGTTTAGCGCTGTTGGATCTGATAGAAGAGGGTAACCTCGGTTTGATCCGTGCGGTGG
AAAAGTTTGATCCTGAACGCGGTTTCCGTTTTTCCACTTACGCGACTTGGTGGATTCGCCAAACGATTGAACGGGCGATT
ATGAACCAAACCCGTACCATCCGTTTGCCTATCCATATTGTTAAAGAATTAAACGTTTATTTACGCACAGCACGAGAGCT
TTCTCATAAATTGGATCATGAACCGAGCGCGGAAGAGATTGCAGAGCAACTCGACAAGCCGGTCGATGATGTGAGCCGTA
TGTTACGCCTTAACGAACGTATCACTTCTGTTGATACACCTTTAGGCGGCGACTCAGAGAAAGCGTTGTTGGATATTCTG
TCTGACGAAAATGAGAACGGTCCGGAAGATACAACACAAGATGACGATATGAAACAGAGTATCGTTAAGTGGTTGTTTGA
ATTGAATGCAAAACAGCGCGAAGTGTTGGCCCGTCGTTTTGGTCTGTTAGGATATGAAGCGGCAACATTGGAAGATGTTG
GCCGAGAAATTGGTTTGACACGCGAGCGTGTTCGCCAGATTCAGGTTGAAGGCTTACGCCGTCTGCGGGAAATCCTGCAA
ACGCAAGGCCTGAGCATCGAGGCGTTGTTCCGCGAATAA

Upstream 100 bases:

>100_bases
CAAGGGGAAATCCGTAAACCCGCTGCGTTACCTTCCGCAGCGATAGATTGGGCAGAATACGCTGTATTCTGCTCGCCGGA
TTCACGGGTAGGAGCAGCAT

Downstream 100 bases:

>100_bases
GTGTGTCTTCAGAATGAAAGGCGGTGAACAGTAAGCTTTGTGCAAAATACAAAGTAAACTTGGCCGATATGAAGCACAAA
AAGAGTCGATGAAACACAAC

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSQSTLKVNELHEDADFDENSTETEIFDEKALVDDEPTESELADDELLAQGVTQRVLDATQLYLGEIGYSPLLTAEEEVY
FARRALRGDVSSRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAI
MNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDIL
SDENENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQ
TQGLSIEALFRE

Sequences:

>Translated_332_residues
MSQSTLKVNELHEDADFDENSTETEIFDEKALVDDEPTESELADDELLAQGVTQRVLDATQLYLGEIGYSPLLTAEEEVY
FARRALRGDVSSRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAI
MNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDIL
SDENENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQ
TQGLSIEALFRE
>Mature_331_residues
SQSTLKVNELHEDADFDENSTETEIFDEKALVDDEPTESELADDELLAQGVTQRVLDATQLYLGEIGYSPLLTAEEEVYF
ARRALRGDVSSRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIM
NQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILS
DENENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQT
QGLSIEALFRE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=332, Percent_Identity=92.4698795180723, Blast_Score=615, Evalue=1e-178,
Organism=Escherichia coli, GI1789448, Length=303, Percent_Identity=38.9438943894389, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1789871, Length=268, Percent_Identity=26.865671641791, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38215; Mature: 38083

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSTLKVNELHEDADFDENSTETEIFDEKALVDDEPTESELADDELLAQGVTQRVLDAT
CCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QLYLGEIGYSPLLTAEEEVYFARRALRGDVSSRRRMIESNLRLVVKIARRYSNRGLALLD
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHH
LIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVY
HHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
LRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDIL
HHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
SDENENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLT
HCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
RERVRQIQVEGLRRLREILQTQGLSIEALFRE
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCC
>Mature Secondary Structure 
SQSTLKVNELHEDADFDENSTETEIFDEKALVDDEPTESELADDELLAQGVTQRVLDAT
CCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QLYLGEIGYSPLLTAEEEVYFARRALRGDVSSRRRMIESNLRLVVKIARRYSNRGLALLD
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHH
LIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVY
HHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
LRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDIL
HHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
SDENENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLT
HCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
RERVRQIQVEGLRRLREILQTQGLSIEALFRE
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]