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The map label for this gene is tyrS [H]

Identifier: 218929460

GI number: 218929460

Start: 2661823

End: 2663097

Strand: Reverse

Name: tyrS [H]

Synonym: YPO2369

Alternate gene names: 218929460

Gene position: 2663097-2661823 (Counterclockwise)

Preceding gene: 218929461

Following gene: 218929459

Centisome position: 57.23

GC content: 48.86

Gene sequence:

>1275_bases
ATGACTAGCAGCAACCTGATTAAACAATTGCAAGAGCGGGGCCTCGTTGCCCAGGTTACGGATGAAGACGCGTTAGCAGA
GAGACTGGCGCAAGGGCCAATTTCACTGTATTGCGGTTTCGATCCTACCGCAGACAGCTTGCACTTGGGTCATCTGGTTC
CCTTGTTATGTCTGAAGCGTTTTCAGTTAGCGGGTCACCGCCCGGTGGCGTTGGTCGGGGGCGCTACCGGTATGATTGGC
GACCCAAGCTTCAAAGCCAGTGAGCGTAAATTGAATACCGAAGATACAGTGAATGAGTGGGTCGAGAAAATCCGCCACCA
GGTCTCGCCATTCCTGGATTTTGATTGCGGTGAAAACAGTGCTATTGCAGCCAATAACTATGATTGGTTTGGTGGCATGA
ATGTGCTCACTTTCCTGCGTGATATTGGCAAACACTTCTCAGTTAATCAGATGATTAATAAAGAAGCCGTCAAGCAGCGC
TTGAACCGTGATGACAGCGGTATCTCGTTTACTGAGTTCTCATACAATTTATTGCAGGCGTATGATTTCGCTTGTTTAAA
CAAAAATCACGGTGTTGCGCTGCAAATCGGTGGTTCTGACCAGTGGGGGAATATTACCTCCGGTATTGACCTGACCCGTC
GCCTCCATCAACAGCAGGTTTATGGCCTGACGGTACCGCTGATCACCAAAGCCGATGGCACTAAATTCGGTAAAACCGAA
GGGGGTGCAGTCTGGCTGGATCCAAAGAAAACCAGTCCTTACAAATTCTACCAATTCTGGATTAATACTGCCGATGCTGA
TGTGTATCGCTTCCTGAAATTCTTTACTTTCATGAGTTTGGAAGAGATTAACGCACTGGAAGAAGAAGATAAAAACAGTG
GTAAAGCACCGCGCGCGCAATATGTTTTGGCGGAAAATGTCACGGGTATGGTGCATGGTCCAGAAGGCTTGGCAGCGGCG
AAACGTATCACTGACAGCCTGTTTTCGGGTGATTTGCATGATATGACCGAAGCAGACTTTGCCCAGTTGGCACAAGATGG
TATGCCGACGGTTGAGCTGAACCGTGATGCTGATTTACAGCAAGCGCTGGTTAATGCTGAGCTGGTTCCTTCCCGTGGTC
AGGCGCGCACGATGATTGGTTCTAATGCCGTGGCCATCAATGGTGAGAAACAGGCTGATCCAGAATACGTCTTTACGGAT
GCGGATCGCCTGTTTGGCCGTTATACTTTGTTGCGCCGTGGCAAAAAACACTATTGCCTGATTAGTTGGCTGTAA

Upstream 100 bases:

>100_bases
TGCCGATTGAAAATATATGCGCGCCGCTTGTGTGGTGTTTCCGTTTAGCTAACACATACTTTGTAAAAGCCAATTTTTGT
AAAAGACAATGGAGTCATTG

Downstream 100 bases:

>100_bases
GCGTAAAATAAGAGAAGGGGCAGAAATGTCCCTTATTTTTTTGCTGAGACCTGACTCTTTTAAGTCAGTGATTCGTGTGA
GCGAAAACGGCTAACACCGC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTSSNLIKQLQERGLVAQVTDEDALAERLAQGPISLYCGFDPTADSLHLGHLVPLLCLKRFQLAGHRPVALVGGATGMIG
DPSFKASERKLNTEDTVNEWVEKIRHQVSPFLDFDCGENSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQR
LNRDDSGISFTEFSYNLLQAYDFACLNKNHGVALQIGGSDQWGNITSGIDLTRRLHQQQVYGLTVPLITKADGTKFGKTE
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSLEEINALEEEDKNSGKAPRAQYVLAENVTGMVHGPEGLAAA
KRITDSLFSGDLHDMTEADFAQLAQDGMPTVELNRDADLQQALVNAELVPSRGQARTMIGSNAVAINGEKQADPEYVFTD
ADRLFGRYTLLRRGKKHYCLISWL

Sequences:

>Translated_424_residues
MTSSNLIKQLQERGLVAQVTDEDALAERLAQGPISLYCGFDPTADSLHLGHLVPLLCLKRFQLAGHRPVALVGGATGMIG
DPSFKASERKLNTEDTVNEWVEKIRHQVSPFLDFDCGENSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQR
LNRDDSGISFTEFSYNLLQAYDFACLNKNHGVALQIGGSDQWGNITSGIDLTRRLHQQQVYGLTVPLITKADGTKFGKTE
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSLEEINALEEEDKNSGKAPRAQYVLAENVTGMVHGPEGLAAA
KRITDSLFSGDLHDMTEADFAQLAQDGMPTVELNRDADLQQALVNAELVPSRGQARTMIGSNAVAINGEKQADPEYVFTD
ADRLFGRYTLLRRGKKHYCLISWL
>Mature_423_residues
TSSNLIKQLQERGLVAQVTDEDALAERLAQGPISLYCGFDPTADSLHLGHLVPLLCLKRFQLAGHRPVALVGGATGMIGD
PSFKASERKLNTEDTVNEWVEKIRHQVSPFLDFDCGENSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQRL
NRDDSGISFTEFSYNLLQAYDFACLNKNHGVALQIGGSDQWGNITSGIDLTRRLHQQQVYGLTVPLITKADGTKFGKTEG
GAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSLEEINALEEEDKNSGKAPRAQYVLAENVTGMVHGPEGLAAAK
RITDSLFSGDLHDMTEADFAQLAQDGMPTVELNRDADLQQALVNAELVPSRGQARTMIGSNAVAINGEKQADPEYVFTDA
DRLFGRYTLLRRGKKHYCLISWL

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=408, Percent_Identity=37.0098039215686, Blast_Score=244, Evalue=1e-64,
Organism=Escherichia coli, GI1787925, Length=423, Percent_Identity=86.2884160756501, Blast_Score=775, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25145802, Length=408, Percent_Identity=35.0490196078431, Blast_Score=227, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI25145805, Length=312, Percent_Identity=33.0128205128205, Blast_Score=162, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6325160, Length=438, Percent_Identity=35.8447488584475, Blast_Score=269, Evalue=7e-73,
Organism=Drosophila melanogaster, GI20130383, Length=446, Percent_Identity=36.5470852017937, Blast_Score=291, Evalue=4e-79,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47159; Mature: 47028

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSNLIKQLQERGLVAQVTDEDALAERLAQGPISLYCGFDPTADSLHLGHLVPLLCLKR
CCCHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCEEHHHHHHHHHHHH
FQLAGHRPVALVGGATGMIGDPSFKASERKLNTEDTVNEWVEKIRHQVSPFLDFDCGENS
HHHCCCCCEEEECCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCC
AIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDSGISFTEFSYNLLQA
EEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHH
YDFACLNKNHGVALQIGGSDQWGNITSGIDLTRRLHQQQVYGLTVPLITKADGTKFGKTE
HHHHEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCC
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSLEEINALEEEDKNSGKAPRAQ
CCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
YVLAENVTGMVHGPEGLAAAKRITDSLFSGDLHDMTEADFAQLAQDGMPTVELNRDADLQ
EEEECCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHH
QALVNAELVPSRGQARTMIGSNAVAINGEKQADPEYVFTDADRLFGRYTLLRRGKKHYCL
HHHHCCCCCCCCCCCEEEECCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEE
ISWL
EEEC
>Mature Secondary Structure 
TSSNLIKQLQERGLVAQVTDEDALAERLAQGPISLYCGFDPTADSLHLGHLVPLLCLKR
CCHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCEEHHHHHHHHHHHH
FQLAGHRPVALVGGATGMIGDPSFKASERKLNTEDTVNEWVEKIRHQVSPFLDFDCGENS
HHHCCCCCEEEECCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCC
AIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDSGISFTEFSYNLLQA
EEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHH
YDFACLNKNHGVALQIGGSDQWGNITSGIDLTRRLHQQQVYGLTVPLITKADGTKFGKTE
HHHHEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCC
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSLEEINALEEEDKNSGKAPRAQ
CCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
YVLAENVTGMVHGPEGLAAAKRITDSLFSGDLHDMTEADFAQLAQDGMPTVELNRDADLQ
EEEECCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHH
QALVNAELVPSRGQARTMIGSNAVAINGEKQADPEYVFTDADRLFGRYTLLRRGKKHYCL
HHHHCCCCCCCCCCCEEEECCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEE
ISWL
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA