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The map label for this gene is pntA [H]

Identifier: 218929395

GI number: 218929395

Start: 2590044

End: 2591570

Strand: Reverse

Name: pntA [H]

Synonym: YPO2303

Alternate gene names: 218929395

Gene position: 2591570-2590044 (Counterclockwise)

Preceding gene: 218929396

Following gene: 218929394

Centisome position: 55.69

GC content: 51.34

Gene sequence:

>1527_bases
ATGCGTATTGGTGTACCAAGAGAGCGGTTGGCCAATGAAGCCAGGGTTGCAGCAACGCCGAAAACGGTGGAACAATTGCT
GAAATTAGGCTTCACCGTGGCAATTGAAAGTGGAGCGGGGCATTTAGCCAGCTTTGACGATGCGGCGTACCAAGAAGCGG
GTGCCATCATTACGGATACCACTGATGTGTGGCAATCCGATCTTATCCTGAAGGTGAATGCTCCCTTTGAGGATGAAATT
GCGCTGATGCGTGAGGGGAGCACGTTGGTCAGTTTTATTTGGCCCGCTCAGAACCCTGAGTTGCTGCAAAAACTGGCAGA
GCGTCAAGTTACTGCATTAGCGATGGATTCAGTGCCGCGTATCTCCCGTGCGCAATCCATGGATGCCCTCAGCTCCATGG
CAAATATTGCCGGTTACCGTGCCATTGTTGAAGCGGCTCATGAATTTGGCCGTTTCTTCACCGGGCAGATCACCGCGGCG
GGTAAAGTTCCACCTGCGAAGGTGATGGTTATTGGTGCCGGAGTCGCTGGTCTGGCGGCTATCGGTGCGGCGGGGAGCCT
CGGGGCTATCGTGCGTGCTTTTGACACCCGTCCTGAAGTAAAAGAACAAGTCCAGAGTATGGGCGCTGAATTCCTTGAGT
TGGATTTTGAAGAGGAAGCGGGCAGTGGCGATGGTTACGCCAAAGTGATGTCCGAGGCCTTTATCAAAGCTGAGATGGAA
CTGTTTGCTGCGCAAGCCGCAGAAGTAGACATTATCGTCACTACCGCGCTGATCCCTGGTAGACCAGCACCGCGCCTGAT
CACCAAAGAGATGGTGGCGTCGATGAAGTCGGGCAGCGTGATTGTCGATCTGGCGGCTCAGACAGGCGGTAACTGTGAGT
TGACGGTAGCTGATGAAGTCACCATCACGGAAAATGGCGTAAAAATCATTGGCTATACAGATCTGCCAAGCCGCTTACCG
ACGCAGTCTTCACAGCTCTATGGTACTAACCTCGTTAACTTATTGAAGTTACTTTGCAAAGAGAAAAACGGCGAGATCGA
TATTGATTTCGACGATACCGTGATTCGCGGTGTTACTGTGGTTAAAACTGGCGAAGTGACTTGGCCGGCACCACCTATTC
AGGTTTCAGCTCAGCCGCAGGCTGCCAAAGCGGCCCCAATAGCGAAGGAAGAAACGAAGCCCTCATCACCGTGGACCAAA
TACATCATTATGGCAGTTGCTATTGTGTTGTTTGGTTGGTTGGCGAATGTGGCACCACCAGAATTCTTATCTCACTTTAC
GGTCTTTGCACTGGCGTGTGTAGTCGGTTACTACGTGGTATGGAATGTCAGTCATGCGCTGCATACACCGCTGATGTCAG
TCACTAACGCGATTTCGGGCATTATTGTGGTTGGCGCGTTGTTGCAGATTGGTCATGGTGGCTGGGTAAGTTTCTTCTCC
TTTATTGCCGTACTGATTGCTAGCATCAACATTTTTGGTGGCTTCACTGTCACCCAGCGCATGCTGAAAATGTTCCGTAA
AAACTAA

Upstream 100 bases:

>100_bases
AGGGAGCGTAACTCAGCAGGTTTTGTCGGTGAACAAACAGAACCGAACGATAAGCAGGATGACAATACTCATCATCAAAA
ACAGGTAAAAAGGGGACATT

Downstream 100 bases:

>100_bases
TGTTCCGTAAAAACCAAGCTACATAGAAATTAGCTGGTATAAAAATGTCATATAAAAACAAAGGGGTAACTGATGTCTAG
TGGTCTCGTTACAGCTGCGT

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MRIGVPRERLANEARVAATPKTVEQLLKLGFTVAIESGAGHLASFDDAAYQEAGAIITDTTDVWQSDLILKVNAPFEDEI
ALMREGSTLVSFIWPAQNPELLQKLAERQVTALAMDSVPRISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEME
LFAAQAAEVDIIVTTALIPGRPAPRLITKEMVASMKSGSVIVDLAAQTGGNCELTVADEVTITENGVKIIGYTDLPSRLP
TQSSQLYGTNLVNLLKLLCKEKNGEIDIDFDDTVIRGVTVVKTGEVTWPAPPIQVSAQPQAAKAAPIAKEETKPSSPWTK
YIIMAVAIVLFGWLANVAPPEFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWVSFFS
FIAVLIASINIFGGFTVTQRMLKMFRKN

Sequences:

>Translated_508_residues
MRIGVPRERLANEARVAATPKTVEQLLKLGFTVAIESGAGHLASFDDAAYQEAGAIITDTTDVWQSDLILKVNAPFEDEI
ALMREGSTLVSFIWPAQNPELLQKLAERQVTALAMDSVPRISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEME
LFAAQAAEVDIIVTTALIPGRPAPRLITKEMVASMKSGSVIVDLAAQTGGNCELTVADEVTITENGVKIIGYTDLPSRLP
TQSSQLYGTNLVNLLKLLCKEKNGEIDIDFDDTVIRGVTVVKTGEVTWPAPPIQVSAQPQAAKAAPIAKEETKPSSPWTK
YIIMAVAIVLFGWLANVAPPEFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWVSFFS
FIAVLIASINIFGGFTVTQRMLKMFRKN
>Mature_508_residues
MRIGVPRERLANEARVAATPKTVEQLLKLGFTVAIESGAGHLASFDDAAYQEAGAIITDTTDVWQSDLILKVNAPFEDEI
ALMREGSTLVSFIWPAQNPELLQKLAERQVTALAMDSVPRISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEME
LFAAQAAEVDIIVTTALIPGRPAPRLITKEMVASMKSGSVIVDLAAQTGGNCELTVADEVTITENGVKIIGYTDLPSRLP
TQSSQLYGTNLVNLLKLLCKEKNGEIDIDFDDTVIRGVTVVKTGEVTWPAPPIQVSAQPQAAKAAPIAKEETKPSSPWTK
YIIMAVAIVLFGWLANVAPPEFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWVSFFS
FIAVLIASINIFGGFTVTQRMLKMFRKN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=532, Percent_Identity=51.1278195488722, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI122939153, Length=532, Percent_Identity=51.1278195488722, Blast_Score=488, Evalue=1e-138,
Organism=Escherichia coli, GI1787887, Length=510, Percent_Identity=83.7254901960784, Blast_Score=872, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=518, Percent_Identity=50, Blast_Score=474, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 54271; Mature: 54271

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGVPRERLANEARVAATPKTVEQLLKLGFTVAIESGAGHLASFDDAAYQEAGAIITDT
CCCCCCHHHHCCCHHCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCCEEECC
TDVWQSDLILKVNAPFEDEIALMREGSTLVSFIWPAQNPELLQKLAERQVTALAMDSVPR
HHHHCCCEEEEECCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCH
ISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEME
HCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHEECCHHCCCCCCHHHHHHHHHHHHHHHH
LFAAQAAEVDIIVTTALIPGRPAPRLITKEMVASMKSGSVIVDLAAQTGGNCELTVADEV
HHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCE
TITENGVKIIGYTDLPSRLPTQSSQLYGTNLVNLLKLLCKEKNGEIDIDFDDTVIRGVTV
EEECCCEEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCEEECCCCHHHCCEEE
VKTGEVTWPAPPIQVSAQPQAAKAAPIAKEETKPSSPWTKYIIMAVAIVLFGWLANVAPP
EEECCEECCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
EFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWVSFFS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGVPRERLANEARVAATPKTVEQLLKLGFTVAIESGAGHLASFDDAAYQEAGAIITDT
CCCCCCHHHHCCCHHCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCCEEECC
TDVWQSDLILKVNAPFEDEIALMREGSTLVSFIWPAQNPELLQKLAERQVTALAMDSVPR
HHHHCCCEEEEECCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCH
ISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEME
HCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHEECCHHCCCCCCHHHHHHHHHHHHHHHH
LFAAQAAEVDIIVTTALIPGRPAPRLITKEMVASMKSGSVIVDLAAQTGGNCELTVADEV
HHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCE
TITENGVKIIGYTDLPSRLPTQSSQLYGTNLVNLLKLLCKEKNGEIDIDFDDTVIRGVTV
EEECCCEEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCEEECCCCHHHCCEEE
VKTGEVTWPAPPIQVSAQPQAAKAAPIAKEETKPSSPWTKYIIMAVAIVLFGWLANVAPP
EEECCEECCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
EFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWVSFFS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]