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The map label for this gene is pflA [H]

Identifier: 218928529

GI number: 218928529

Start: 1555542

End: 1556276

Strand: Reverse

Name: pflA [H]

Synonym: YPO1381

Alternate gene names: 218928529

Gene position: 1556276-1555542 (Counterclockwise)

Preceding gene: 218928531

Following gene: 218928522

Centisome position: 33.44

GC content: 46.67

Gene sequence:

>735_bases
GTGCTTGGTCGTATTCATTCATTCGAATCCTGTGGCACTGTTGATGGCCCAGGTATTAGGTTTATCGTCTTCTTCCAAGG
CTGCCTAATGCGCTGCCTGTATTGTCACAACAGAGATACCTGGGACACCCACGGCGGCAAGGAAGTGACCGTTGAAGAAT
TAGTTAAAGAAGCAGTCACCTATCGCCACTTTATGAATGCTTCGGGTGGCGGTGTCACTGCCTCTGGTGGCGAGGCCATA
TTGCAGGCTGAGTTTGTCCGCGATTGGTTCCGAGCCTGTCACAAAGAAGGCATTCACACCTGTTTGGATACCAATGGCTT
TGTACGCCGGTATGATCCCGTCATTGATGAGTTGTTGGATGCAACAGATTTAGTGATGTTAGATTTAAAACAGATGGATG
ACAGCATTCACCAGAACTTGGTTGGCGTATCCAATCACCGGACTTTAGAGTTTGCCCGTTATCTGGCAAAACGTAACCAG
AAAACCTGGATCCGCTATGTCGTGGTGCCAGGCTGGTCCGACGATGATAAATCAGCACATATGCTGGGTGAATTTACTCA
GAACATGAGTAATATCGAAAAAATAGAATTACTGCCTTACCACGAACTGGGTAAGCACAAATGGATTGCTATGGGGGAAG
AATACAAGCTTGATGGCGTAAAACCGCCAACAAAAGAGATTATGGATCGCGTGAAAGGTATTTTGGAAGGCTATGGCCAC
AAAGTCATCTACTGA

Upstream 100 bases:

>100_bases
CTACCATTCAGCACCAAACAGTACTAACTGGAACTTTATGAACAAGATAACCGATTGTATTACAACAGACCCCTCCGATC
TCGTAGAGGATAAGAAGCCT

Downstream 100 bases:

>100_bases
TTTAGAACCCTGTATTGATGCATGTTATTGGCTAAAGTGTACTGTTAACTCATGTGTATTAGGTTTGGCCTAACAACTGC
CGCCATGCTGGATGAATCCG

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MLGRIHSFESCGTVDGPGIRFIVFFQGCLMRCLYCHNRDTWDTHGGKEVTVEELVKEAVTYRHFMNASGGGVTASGGEAI
LQAEFVRDWFRACHKEGIHTCLDTNGFVRRYDPVIDELLDATDLVMLDLKQMDDSIHQNLVGVSNHRTLEFARYLAKRNQ
KTWIRYVVVPGWSDDDKSAHMLGEFTQNMSNIEKIELLPYHELGKHKWIAMGEEYKLDGVKPPTKEIMDRVKGILEGYGH
KVIY

Sequences:

>Translated_244_residues
MLGRIHSFESCGTVDGPGIRFIVFFQGCLMRCLYCHNRDTWDTHGGKEVTVEELVKEAVTYRHFMNASGGGVTASGGEAI
LQAEFVRDWFRACHKEGIHTCLDTNGFVRRYDPVIDELLDATDLVMLDLKQMDDSIHQNLVGVSNHRTLEFARYLAKRNQ
KTWIRYVVVPGWSDDDKSAHMLGEFTQNMSNIEKIELLPYHELGKHKWIAMGEEYKLDGVKPPTKEIMDRVKGILEGYGH
KVIY
>Mature_244_residues
MLGRIHSFESCGTVDGPGIRFIVFFQGCLMRCLYCHNRDTWDTHGGKEVTVEELVKEAVTYRHFMNASGGGVTASGGEAI
LQAEFVRDWFRACHKEGIHTCLDTNGFVRRYDPVIDELLDATDLVMLDLKQMDDSIHQNLVGVSNHRTLEFARYLAKRNQ
KTWIRYVVVPGWSDDDKSAHMLGEFTQNMSNIEKIELLPYHELGKHKWIAMGEEYKLDGVKPPTKEIMDRVKGILEGYGH
KVIY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=244, Percent_Identity=88.1147540983607, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1790389, Length=274, Percent_Identity=26.2773722627737, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI226510931, Length=188, Percent_Identity=29.7872340425532, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27912; Mature: 27912

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGRIHSFESCGTVDGPGIRFIVFFQGCLMRCLYCHNRDTWDTHGGKEVTVEELVKEAVT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
YRHFMNASGGGVTASGGEAILQAEFVRDWFRACHKEGIHTCLDTNGFVRRYDPVIDELLD
HHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHC
ATDLVMLDLKQMDDSIHQNLVGVSNHRTLEFARYLAKRNQKTWIRYVVVPGWSDDDKSAH
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHH
MLGEFTQNMSNIEKIELLPYHELGKHKWIAMGEEYKLDGVKPPTKEIMDRVKGILEGYGH
HHHHHHHHHHHHHHEEECCHHHHCCCCEEEECCCEECCCCCCCHHHHHHHHHHHHHHCCC
KVIY
CCCC
>Mature Secondary Structure
MLGRIHSFESCGTVDGPGIRFIVFFQGCLMRCLYCHNRDTWDTHGGKEVTVEELVKEAVT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
YRHFMNASGGGVTASGGEAILQAEFVRDWFRACHKEGIHTCLDTNGFVRRYDPVIDELLD
HHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHC
ATDLVMLDLKQMDDSIHQNLVGVSNHRTLEFARYLAKRNQKTWIRYVVVPGWSDDDKSAH
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHH
MLGEFTQNMSNIEKIELLPYHELGKHKWIAMGEEYKLDGVKPPTKEIMDRVKGILEGYGH
HHHHHHHHHHHHHHEEECCHHHHCCCCEEEECCCEECCCCCCCHHHHHHHHHHHHHHCCC
KVIY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]