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The map label for this gene is rsuA

Identifier: 218928420

GI number: 218928420

Start: 1423503

End: 1424210

Strand: Direct

Name: rsuA

Synonym: YPO1266

Alternate gene names: 218928420

Gene position: 1423503-1424210 (Clockwise)

Preceding gene: 218928417

Following gene: 218928421

Centisome position: 30.59

GC content: 51.98

Gene sequence:

>708_bases
ATGCGATTGGACAAGTTTTTATCTCAGCAATTAGGGGTCAGCCGGGCGTTGGTAGCGCGTGAACTGCGGGCAAAGCGTGT
GACTATTGATGGCGAGGTGGTCAAAAGTGGTGCCATCAAGCTGACACCGGAACAAGACGTGCGATTTGATGGCAATCCGT
TACAGCAGATGGTTGGCCCCCGTTATTTTATGTTGAACAAACCGCAGGGCTATGTGTGTTCGACGGAAGATCCCGATCAT
CCAACGGTGTTGTATTTCCTTGATGAGCCAGTGGCTTACAAACTCCATGCCGCTGGGCGGTTGGATATCGATACTACCGG
GTTGGTCTTGATGACTGACGATGGGCAGTGGTCACATCGCGTCACTTCGCCACGTCATCATTGTGAAAAGACCTATTTGG
TGACGTTGGAACATCCGCTATCCGATGACACCGCACAGCAATTTGCTGACGGTGTCCAACTGTACAATGAAAAATCCCTG
ACCAAACCGGCTCAACTGGACGTTATCGAGCCGCAACGGGTGCGTTTGACCATCAGCGAAGGGCGTTATCATCAGGTGAA
GCGGATGTTTGCTGCTGTGGGGAACCGAGTGATTGAGTTACATCGTGAACGCATTGGTGATATCACGCTTGATGATGATT
TAGCCCCTGGTGAGTACCGGCCGCTGACGACAGAAGAGATCGCCAGCGTGGGTGCCCCACACTTGTAA

Upstream 100 bases:

>100_bases
TTTCAACCCGTACAATTGACATCCTATTATCATCAACGGGTGTCATTATGGGGACCGTATCCATCAGTGATACGGCCATA
CAACAGGCAAAATAGATTCA

Downstream 100 bases:

>100_bases
TGAGATTGTTTGTAATGAGATTGTTTGTAATGAGATTGTTATTGCCGTGCGGGCCAGCGGTTCCGCACCGTTCTACTGCG
GTATAATCTGCGGTAAGTTT

Product: 16S rRNA pseudouridylate synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MRLDKFLSQQLGVSRALVARELRAKRVTIDGEVVKSGAIKLTPEQDVRFDGNPLQQMVGPRYFMLNKPQGYVCSTEDPDH
PTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHRVTSPRHHCEKTYLVTLEHPLSDDTAQQFADGVQLYNEKSL
TKPAQLDVIEPQRVRLTISEGRYHQVKRMFAAVGNRVIELHRERIGDITLDDDLAPGEYRPLTTEEIASVGAPHL

Sequences:

>Translated_235_residues
MRLDKFLSQQLGVSRALVARELRAKRVTIDGEVVKSGAIKLTPEQDVRFDGNPLQQMVGPRYFMLNKPQGYVCSTEDPDH
PTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHRVTSPRHHCEKTYLVTLEHPLSDDTAQQFADGVQLYNEKSL
TKPAQLDVIEPQRVRLTISEGRYHQVKRMFAAVGNRVIELHRERIGDITLDDDLAPGEYRPLTTEEIASVGAPHL
>Mature_235_residues
MRLDKFLSQQLGVSRALVARELRAKRVTIDGEVVKSGAIKLTPEQDVRFDGNPLQQMVGPRYFMLNKPQGYVCSTEDPDH
PTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHRVTSPRHHCEKTYLVTLEHPLSDDTAQQFADGVQLYNEKSL
TKPAQLDVIEPQRVRLTISEGRYHQVKRMFAAVGNRVIELHRERIGDITLDDDLAPGEYRPLTTEEIASVGAPHL

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=82.1739130434783, Blast_Score=402, Evalue=1e-114,
Organism=Escherichia coli, GI1787524, Length=231, Percent_Identity=32.034632034632, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=27.4678111587983, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=32.967032967033, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSUA_YERPE (Q8ZGM2)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AI0154
- RefSeq:   NP_670217.1
- RefSeq:   NP_992688.1
- RefSeq:   YP_002346295.1
- ProteinModelPortal:   Q8ZGM2
- SMR:   Q8ZGM2
- GeneID:   1147864
- GeneID:   1174112
- GeneID:   2766295
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1266
- KEGG:   ypk:y2917
- KEGG:   ypm:YP_1322
- HOGENOM:   HBG754478
- OMA:   GLHRWKI
- ProtClustDB:   PRK10839
- BioCyc:   YPES187410:Y2917-MONOMER
- BioCyc:   YPES214092:YPO1266-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26542; Mature: 26542

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 102-102

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFLSQQLGVSRALVARELRAKRVTIDGEVVKSGAIKLTPEQDVRFDGNPLQQMVGP
CCHHHHHHHHHCHHHHHHHHHHHHHEEEECHHEECCCCEEECCCCCCCCCCCHHHHHCCC
RYFMLNKPQGYVCSTEDPDHPTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHR
EEEEEECCCCCEECCCCCCCCEEEEEECCCEEEEEECCCEEEECCCCEEEEECCCCCCCC
VTSPRHHCEKTYLVTLEHPLSDDTAQQFADGVQLYNEKSLTKPAQLDVIEPQRVRLTISE
CCCCHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEECC
GRYHQVKRMFAAVGNRVIELHRERIGDITLDDDLAPGEYRPLTTEEIASVGAPHL
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHCCCCCC
>Mature Secondary Structure
MRLDKFLSQQLGVSRALVARELRAKRVTIDGEVVKSGAIKLTPEQDVRFDGNPLQQMVGP
CCHHHHHHHHHCHHHHHHHHHHHHHEEEECHHEECCCCEEECCCCCCCCCCCHHHHHCCC
RYFMLNKPQGYVCSTEDPDHPTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHR
EEEEEECCCCCEECCCCCCCCEEEEEECCCEEEEEECCCEEEECCCCEEEEECCCCCCCC
VTSPRHHCEKTYLVTLEHPLSDDTAQQFADGVQLYNEKSLTKPAQLDVIEPQRVRLTISE
CCCCHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEECC
GRYHQVKRMFAAVGNRVIELHRERIGDITLDDDLAPGEYRPLTTEEIASVGAPHL
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430