Click here to switch to the map view.

The map label for this gene is moaA

Identifier: 218928323

GI number: 218928323

Start: 1306853

End: 1307833

Strand: Direct

Name: moaA

Synonym: YPO1159

Alternate gene names: 218928323

Gene position: 1306853-1307833 (Clockwise)

Preceding gene: 218928321

Following gene: 218928324

Centisome position: 28.08

GC content: 49.24

Gene sequence:

>981_bases
ATGGTACAACTCACTGACGCATTTGCGCGTAAATTCTACTATTTGCGCCTATCGATCACTGATGTGTGCAATTTCCGTTG
CACATATTGCTTGCCAGAGGGCTACCGCCCAGATGGTGTCAAAAGTTTTTTGAGCCTTGACGAAATCAATCGCGTCAGCC
GCGCCTTTGCCTTGTTGGGAACGGAAAAAATCCGCCTGACGGGGGGCGAGCCTTCGATGCGGCGCGACTTCACTGATATT
ATTGCCACTATCCGGCAAAATCCCGCTATCCGTACCTTGGCCGTTACAACTAATGGTTATCGTTTAGTTCGTGATGTGGC
GCAATGGCGTGATGCGGGCCTGACGGCAATTAATGTCAGCGTCGATAGCCTGGATCCGCGACAATTTCATGCCATCACCG
GGCAGGATAAATTTTATCAAGTTATGCAGGGCATTGATGCCGCATTTGATGCCGGTTTTGACAAAGTCAAAGTCAACGCT
GTTTTGATGCGCGATGTTAATGATCGCCAACTGAGTGCATTCCTTGACTGGATCAAACCTCGGCCCATTCAACTGCGTTT
TATCGAGCTGATGGAAACCGGCGAAGGCGGTAACCTGTTCCGTAAACACCACGTTTCTGGTGGCGTCATCCGCCAACAAT
TACTCGAACAAGGCTGGCAGCAGCAAGATCGGGCGCGCAGTGATGGCCCGGCTCAGGTGTTTCACCACTCGGATTACCAA
GGTGAAATTGGGCTTATCATGCCGTATGAAAAAGACTTTTGCGCCAGTTGTAACCGCCTGCGTGTTTCTGCTCTCGGTAA
TTTGCATTTGTGCTTATTTGGTGAGCAGGGGATTACATTACGTGATCTGCTGGGCAGTGATGATCAACAAGATGAGCTGA
TAGCGCGGATTCAAAGTGCGTTGCAAACGAAGAAACAGACTCATTTTTTGCATCAGGGTAACAGCGGTATAACGCAAAAT
TTATCCTTTATTGGCGGCTGA

Upstream 100 bases:

>100_bases
TTGTCTTTTTTGTATTGGCAATATGATGTTCTGGCCGTGGCCTTAAGCCACCAGGGTGCAAGGTAGAAATGCCTGCATCT
CCCGTATTTGGAAAGGTGTT

Downstream 100 bases:

>100_bases
TGTGAGCGAATTAGCGCCGATGAGCTTACATCAGTAAGTGATTCGGGCAAGTGAATGCCGCTAACCCCCCTGTAATGTCA
AGAACGAAGGGATTTTATTG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MVQLTDAFARKFYYLRLSITDVCNFRCTYCLPEGYRPDGVKSFLSLDEINRVSRAFALLGTEKIRLTGGEPSMRRDFTDI
IATIRQNPAIRTLAVTTNGYRLVRDVAQWRDAGLTAINVSVDSLDPRQFHAITGQDKFYQVMQGIDAAFDAGFDKVKVNA
VLMRDVNDRQLSAFLDWIKPRPIQLRFIELMETGEGGNLFRKHHVSGGVIRQQLLEQGWQQQDRARSDGPAQVFHHSDYQ
GEIGLIMPYEKDFCASCNRLRVSALGNLHLCLFGEQGITLRDLLGSDDQQDELIARIQSALQTKKQTHFLHQGNSGITQN
LSFIGG

Sequences:

>Translated_326_residues
MVQLTDAFARKFYYLRLSITDVCNFRCTYCLPEGYRPDGVKSFLSLDEINRVSRAFALLGTEKIRLTGGEPSMRRDFTDI
IATIRQNPAIRTLAVTTNGYRLVRDVAQWRDAGLTAINVSVDSLDPRQFHAITGQDKFYQVMQGIDAAFDAGFDKVKVNA
VLMRDVNDRQLSAFLDWIKPRPIQLRFIELMETGEGGNLFRKHHVSGGVIRQQLLEQGWQQQDRARSDGPAQVFHHSDYQ
GEIGLIMPYEKDFCASCNRLRVSALGNLHLCLFGEQGITLRDLLGSDDQQDELIARIQSALQTKKQTHFLHQGNSGITQN
LSFIGG
>Mature_326_residues
MVQLTDAFARKFYYLRLSITDVCNFRCTYCLPEGYRPDGVKSFLSLDEINRVSRAFALLGTEKIRLTGGEPSMRRDFTDI
IATIRQNPAIRTLAVTTNGYRLVRDVAQWRDAGLTAINVSVDSLDPRQFHAITGQDKFYQVMQGIDAAFDAGFDKVKVNA
VLMRDVNDRQLSAFLDWIKPRPIQLRFIELMETGEGGNLFRKHHVSGGVIRQQLLEQGWQQQDRARSDGPAQVFHHSDYQ
GEIGLIMPYEKDFCASCNRLRVSALGNLHLCLFGEQGITLRDLLGSDDQQDELIARIQSALQTKKQTHFLHQGNSGITQN
LSFIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=311, Percent_Identity=39.5498392282958, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI22027536, Length=311, Percent_Identity=39.5498392282958, Blast_Score=227, Evalue=1e-59,
Organism=Escherichia coli, GI1786999, Length=326, Percent_Identity=78.2208588957055, Blast_Score=532, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI32566331, Length=307, Percent_Identity=38.4364820846906, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI32566329, Length=307, Percent_Identity=38.4364820846906, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17561210, Length=307, Percent_Identity=37.785016286645, Blast_Score=207, Evalue=6e-54,
Organism=Drosophila melanogaster, GI28574490, Length=310, Percent_Identity=37.0967741935484, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI28574488, Length=310, Percent_Identity=37.0967741935484, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_YERPE (Q8ZGW5)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AE0142
- RefSeq:   NP_670322.1
- RefSeq:   NP_992374.1
- RefSeq:   YP_002346198.1
- ProteinModelPortal:   Q8ZGW5
- SMR:   Q8ZGW5
- GeneID:   1147969
- GeneID:   1174008
- GeneID:   2764835
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1159
- KEGG:   ypk:y3023
- KEGG:   ypm:YP_1000
- HOGENOM:   HBG529885
- OMA:   KHHVSGV
- ProtClustDB:   PRK00164
- BioCyc:   YPES187410:Y3023-MONOMER
- BioCyc:   YPES214092:YPO1159-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 36846; Mature: 36846

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 16-16 BINDING 29-29 BINDING 65-65 BINDING 69-69 BINDING 96-96 BINDING 120-120 BINDING 157-157 BINDING 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQLTDAFARKFYYLRLSITDVCNFRCTYCLPEGYRPDGVKSFLSLDEINRVSRAFALLG
CCCCHHHHHCEEEEEEEEEEHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
TEKIRLTGGEPSMRRDFTDIIATIRQNPAIRTLAVTTNGYRLVRDVAQWRDAGLTAINVS
CCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEE
VDSLDPRQFHAITGQDKFYQVMQGIDAAFDAGFDKVKVNAVLMRDVNDRQLSAFLDWIKP
ECCCCCHHEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHCC
RPIQLRFIELMETGEGGNLFRKHHVSGGVIRQQLLEQGWQQQDRARSDGPAQVFHHSDYQ
CCEEEEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
GEIGLIMPYEKDFCASCNRLRVSALGNLHLCLFGEQGITLRDLLGSDDQQDELIARIQSA
CCEEEEECCCCHHHCCCCCEEEEECCCEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHH
LQTKKQTHFLHQGNSGITQNLSFIGG
HHHHHHHHHHHCCCCCCCCCCHHCCC
>Mature Secondary Structure
MVQLTDAFARKFYYLRLSITDVCNFRCTYCLPEGYRPDGVKSFLSLDEINRVSRAFALLG
CCCCHHHHHCEEEEEEEEEEHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
TEKIRLTGGEPSMRRDFTDIIATIRQNPAIRTLAVTTNGYRLVRDVAQWRDAGLTAINVS
CCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEE
VDSLDPRQFHAITGQDKFYQVMQGIDAAFDAGFDKVKVNAVLMRDVNDRQLSAFLDWIKP
ECCCCCHHEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHCC
RPIQLRFIELMETGEGGNLFRKHHVSGGVIRQQLLEQGWQQQDRARSDGPAQVFHHSDYQ
CCEEEEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
GEIGLIMPYEKDFCASCNRLRVSALGNLHLCLFGEQGITLRDLLGSDDQQDELIARIQSA
CCEEEEECCCCHHHCCCCCEEEEECCCEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHH
LQTKKQTHFLHQGNSGITQNLSFIGG
HHHHHHHHHHHCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430