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The map label for this gene is gutB [H]

Identifier: 15922672

GI number: 15922672

Start: 2342463

End: 2343482

Strand: Direct

Name: gutB [H]

Synonym: ST2341

Alternate gene names: 15922672

Gene position: 2342463-2343482 (Clockwise)

Preceding gene: 15922667

Following gene: 15922675

Centisome position: 86.93

GC content: 36.67

Gene sequence:

>1020_bases
GTGAAAGCTGTACTTTTAGAGAACGGAAAGGTTGTAATTAAGGAGATACCTAAGCCTATAATTAAGGATAAAGGTGATGT
TATAGTTAAGATGAAAGCTTGCGGATTATGTGGTACAGATGTAGAAAAAATTTGTGGACAATACACAGCATCTCAGCCAA
TACTAGGGCATGAGCCAGCTGGAATAATAGATGAATCTAGTGTAGATTTCTTAAAATCAGGACAAAGAGTATTTGCTCAC
CACCATGTTCCTTGCTATGAGTGTTACTATTGTACTCACGGTAGTCCTACAATGTGTCCTTATTATAGGAAAACAAATCT
TGACCCTGGCGGATTTGCTGAGTACTTTAGGGTACCAGCTTGGAATGTAATAAGGGGAGGAATTTTAATTTTACCAGACT
CTGTATCTTTCGATGAAGGAGCTTTTATAGAACCATTAGCTACTGTTGTGAGAGCACAGAGAAGAGTAAAAATAAATAAG
GGAGATAGTGTCTTAGTGGTTGGAGCTGGACCTATGGGTTTATTACACGTAATGATGGCTAAGGCTAATGGTGCCAGTAC
TGTTATAACATCTGACGTATCAGATTTTAGAATTGAATATGCTACCAAAGTAGGAGCAGACTACTCGTTAAATGCTAGAA
AGACTGATATACCAAGTGAAGTTAAGAAATTAACAGACGGTAGAGGAGCTGATGTAGCAATAATAGCCTCGGGGTCTCCT
TCAGCTATATTATCTGGACTACTTTCAGTAAGAAAAGGGGGATACGTATTACTATTTGGTGTTCCGTATAAAGGAACAGT
CTTGAATTACGATATTAGTGAGCTTTTAAATAATGAAATTTCAATAATATCAAGTAATGCTGCAGTGGAAGAAGATACAA
AAGAAGCACTGAAATTAATTTATGAAAGAAAAGTTGATGTCAAAAAACTGATAACCCATAGGTTTCCATTAGATGAATTT
AATGAGGCCGTAAGGGTAGCTAAGCAAGGAGAGGCCATAAAGGTAATAATATATGATTGA

Upstream 100 bases:

>100_bases
TAATTAAATTCACAATAATTGTTACGCGGAAATACTATATATCTCTTTTAGTTTCGCATAAGAAGAAAGAGATTTATTTT
AAGCTCATTACTAACAACTT

Downstream 100 bases:

>100_bases
GATTAAGGTAAAATAAAGTACTAAGGTTGCAACAAAAAGTCTTAATGCTCTTCTTACGTCATCTTCTTTAGGCAAATTAC
CTTCGCCTACTGAATAGTAA

Product: sorbitol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKAVLLENGKVVIKEIPKPIIKDKGDVIVKMKACGLCGTDVEKICGQYTASQPILGHEPAGIIDESSVDFLKSGQRVFAH
HHVPCYECYYCTHGSPTMCPYYRKTNLDPGGFAEYFRVPAWNVIRGGILILPDSVSFDEGAFIEPLATVVRAQRRVKINK
GDSVLVVGAGPMGLLHVMMAKANGASTVITSDVSDFRIEYATKVGADYSLNARKTDIPSEVKKLTDGRGADVAIIASGSP
SAILSGLLSVRKGGYVLLFGVPYKGTVLNYDISELLNNEISIISSNAAVEEDTKEALKLIYERKVDVKKLITHRFPLDEF
NEAVRVAKQGEAIKVIIYD

Sequences:

>Translated_339_residues
MKAVLLENGKVVIKEIPKPIIKDKGDVIVKMKACGLCGTDVEKICGQYTASQPILGHEPAGIIDESSVDFLKSGQRVFAH
HHVPCYECYYCTHGSPTMCPYYRKTNLDPGGFAEYFRVPAWNVIRGGILILPDSVSFDEGAFIEPLATVVRAQRRVKINK
GDSVLVVGAGPMGLLHVMMAKANGASTVITSDVSDFRIEYATKVGADYSLNARKTDIPSEVKKLTDGRGADVAIIASGSP
SAILSGLLSVRKGGYVLLFGVPYKGTVLNYDISELLNNEISIISSNAAVEEDTKEALKLIYERKVDVKKLITHRFPLDEF
NEAVRVAKQGEAIKVIIYD
>Mature_339_residues
MKAVLLENGKVVIKEIPKPIIKDKGDVIVKMKACGLCGTDVEKICGQYTASQPILGHEPAGIIDESSVDFLKSGQRVFAH
HHVPCYECYYCTHGSPTMCPYYRKTNLDPGGFAEYFRVPAWNVIRGGILILPDSVSFDEGAFIEPLATVVRAQRRVKINK
GDSVLVVGAGPMGLLHVMMAKANGASTVITSDVSDFRIEYATKVGADYSLNARKTDIPSEVKKLTDGRGADVAIIASGSP
SAILSGLLSVRKGGYVLLFGVPYKGTVLNYDISELLNNEISIISSNAAVEEDTKEALKLIYERKVDVKKLITHRFPLDEF
NEAVRVAKQGEAIKVIIYD

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=336, Percent_Identity=31.25, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI156523966, Length=343, Percent_Identity=26.2390670553936, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4501939, Length=337, Percent_Identity=26.1127596439169, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1790045, Length=335, Percent_Identity=28.0597014925373, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87082125, Length=357, Percent_Identity=31.6526610644258, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788407, Length=349, Percent_Identity=29.512893982808, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI226510992, Length=332, Percent_Identity=31.3253012048193, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1788075, Length=342, Percent_Identity=27.7777777777778, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788073, Length=335, Percent_Identity=27.7611940298507, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787863, Length=341, Percent_Identity=28.4457478005865, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1786825, Length=379, Percent_Identity=25.3298153034301, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790718, Length=332, Percent_Identity=26.5060240963855, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081918, Length=287, Percent_Identity=29.9651567944251, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562876, Length=344, Percent_Identity=31.9767441860465, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17562878, Length=341, Percent_Identity=30.2052785923754, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17562584, Length=299, Percent_Identity=26.7558528428094, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319955, Length=354, Percent_Identity=32.4858757062147, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6322619, Length=354, Percent_Identity=32.2033898305085, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6323099, Length=339, Percent_Identity=29.4985250737463, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6319258, Length=225, Percent_Identity=29.7777777777778, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320033, Length=353, Percent_Identity=23.2294617563739, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319257, Length=351, Percent_Identity=23.6467236467236, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=322, Percent_Identity=27.9503105590062, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319621, Length=303, Percent_Identity=28.3828382838284, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324486, Length=313, Percent_Identity=27.1565495207668, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319520, Length=226, Percent_Identity=26.1061946902655, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17137530, Length=352, Percent_Identity=30.3977272727273, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI17737897, Length=354, Percent_Identity=30.7909604519774, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 36910; Mature: 36910

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVLLENGKVVIKEIPKPIIKDKGDVIVKMKACGLCGTDVEKICGQYTASQPILGHEPA
CCEEEECCCCEEEECCCCHHHCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCC
GIIDESSVDFLKSGQRVFAHHHVPCYECYYCTHGSPTMCPYYRKTNLDPGGFAEYFRVPA
CCCCCCCHHHHHCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCC
WNVIRGGILILPDSVSFDEGAFIEPLATVVRAQRRVKINKGDSVLVVGAGPMGLLHVMMA
CHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHEEEECCCCEEEEECCCCHHHHHHHHH
KANGASTVITSDVSDFRIEYATKVGADYSLNARKTDIPSEVKKLTDGRGADVAIIASGSP
CCCCCCEEEECCCCHHEEEEHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCH
SAILSGLLSVRKGGYVLLFGVPYKGTVLNYDISELLNNEISIISSNAAVEEDTKEALKLI
HHHHHHHHHHCCCCEEEEEECCCCCEEECCCHHHHCCCCEEEEECCCCCCHHHHHHHHHH
YERKVDVKKLITHRFPLDEFNEAVRVAKQGEAIKVIIYD
HHCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEC
>Mature Secondary Structure
MKAVLLENGKVVIKEIPKPIIKDKGDVIVKMKACGLCGTDVEKICGQYTASQPILGHEPA
CCEEEECCCCEEEECCCCHHHCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCC
GIIDESSVDFLKSGQRVFAHHHVPCYECYYCTHGSPTMCPYYRKTNLDPGGFAEYFRVPA
CCCCCCCHHHHHCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCC
WNVIRGGILILPDSVSFDEGAFIEPLATVVRAQRRVKINKGDSVLVVGAGPMGLLHVMMA
CHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHEEEECCCCEEEEECCCCHHHHHHHHH
KANGASTVITSDVSDFRIEYATKVGADYSLNARKTDIPSEVKKLTDGRGADVAIIASGSP
CCCCCCEEEECCCCHHEEEEHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCH
SAILSGLLSVRKGGYVLLFGVPYKGTVLNYDISELLNNEISIISSNAAVEEDTKEALKLI
HHHHHHHHHHCCCCEEEEEECCCCCEEECCCHHHHCCCCEEEEECCCCCCHHHHHHHHHH
YERKVDVKKLITHRFPLDEFNEAVRVAKQGEAIKVIIYD
HHCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]