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The map label for this gene is nadE

Identifier: 15922484

GI number: 15922484

Start: 2155972

End: 2156811

Strand: Direct

Name: nadE

Synonym: ST2159

Alternate gene names: 15922484

Gene position: 2155972-2156811 (Clockwise)

Preceding gene: 15922483

Following gene: 15922485

Centisome position: 80.01

GC content: 31.9

Gene sequence:

>840_bases
ATGATGCCAGAATATGTAAGAAAAAAACTTACCTTAGACTTTGGCCAAGTTACTGATTACATAGTTAGAAGAATAAGAGA
ATACATAGAAGAGAGTAAAAAAGAAGGAGGGATAATAGGCTTAAGTGGTGGAATTGATTCTTCAGTAACAACAATACTCT
TATCAAGAGCTACCAATAACTTCTACATCCTATTAATGCCTACTTCATCGACACCTCAAAAAGACATTGAAGATGCCATG
AAAATAATTAAAATAGTTAATGGTGAAAATAAATATAGTTATATAAACATTGATGAGATTATTAATGAGTTCTCCTCTAT
TGTCAATATATCCGATAAAATAGTAATAGGAAATATAAAAGCTAGGGTTAGAATGACACTATTATATGCGTTTGCTCAAA
AAATGAACTATCTAGTAATAGGAACGGGAGATAAAAGTGAAATAATGCTTGGTTATTTTACTAAATATGGTGATGGAGGA
GTAGATGTATTACCAATTGGAGACTTATATAAAACACAAGTTAGAATGCTAGGTAATTATTTAGGAGTACCAGAAGAAAT
AGTTAAAAAACCACCTTCTCCAGCATTATGGGAGGGACAAACTGCTGAAGGGGAAATTGGTTTAGATTATGAAACTATAG
ATTCTATACTTTACCTTAAGTTTGAGGAAATGAGAGAACCAGAAGAAATAGCCGAAATGACTAAGACTAGTTATGATAAA
GTAATAAAAATTATAAATATGGTGAAAACATCACAACATAAAAGACTCCCACCAGAGATTTTCAGATTAAGTGGAAGGGC
AATTAACTCTGACTGGAGGTATCCAAGGCAATGGGGTTAA

Upstream 100 bases:

>100_bases
ATACTATAATGAGACAATAAATTCAACAGTATTTACAGTAGAAGTTTACTCATATTATTACTTTGCAACATATAAGGTAA
ATGTAAGTAATATTTCTTAG

Downstream 100 bases:

>100_bases
AAGTAGAATTAGCTCAAATAAGACCAAAACTTGGAGATGTAAAATATAATTTAGAGAAACATCAAGAAATAATATCTTCA
TCTTCTGCGGATTGTATAAT

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MMPEYVRKKLTLDFGQVTDYIVRRIREYIEESKKEGGIIGLSGGIDSSVTTILLSRATNNFYILLMPTSSTPQKDIEDAM
KIIKIVNGENKYSYINIDEIINEFSSIVNISDKIVIGNIKARVRMTLLYAFAQKMNYLVIGTGDKSEIMLGYFTKYGDGG
VDVLPIGDLYKTQVRMLGNYLGVPEEIVKKPPSPALWEGQTAEGEIGLDYETIDSILYLKFEEMREPEEIAEMTKTSYDK
VIKIINMVKTSQHKRLPPEIFRLSGRAINSDWRYPRQWG

Sequences:

>Translated_279_residues
MMPEYVRKKLTLDFGQVTDYIVRRIREYIEESKKEGGIIGLSGGIDSSVTTILLSRATNNFYILLMPTSSTPQKDIEDAM
KIIKIVNGENKYSYINIDEIINEFSSIVNISDKIVIGNIKARVRMTLLYAFAQKMNYLVIGTGDKSEIMLGYFTKYGDGG
VDVLPIGDLYKTQVRMLGNYLGVPEEIVKKPPSPALWEGQTAEGEIGLDYETIDSILYLKFEEMREPEEIAEMTKTSYDK
VIKIINMVKTSQHKRLPPEIFRLSGRAINSDWRYPRQWG
>Mature_279_residues
MMPEYVRKKLTLDFGQVTDYIVRRIREYIEESKKEGGIIGLSGGIDSSVTTILLSRATNNFYILLMPTSSTPQKDIEDAM
KIIKIVNGENKYSYINIDEIINEFSSIVNISDKIVIGNIKARVRMTLLYAFAQKMNYLVIGTGDKSEIMLGYFTKYGDGG
VDVLPIGDLYKTQVRMLGNYLGVPEEIVKKPPSPALWEGQTAEGEIGLDYETIDSILYLKFEEMREPEEIAEMTKTSYDK
VIKIINMVKTSQHKRLPPEIFRLSGRAINSDWRYPRQWG

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=239, Percent_Identity=29.7071129707113, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_SULTO (Q96YL5)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_378153.1
- ProteinModelPortal:   Q96YL5
- SMR:   Q96YL5
- GeneID:   1460230
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST2159
- NMPDR:   fig|273063.1.peg.2321
- HOGENOM:   HBG351567
- OMA:   FRYARDW
- ProtClustDB:   PRK13980
- BioCyc:   STOK273063:ST2159-MONOMER
- BRENDA:   6.3.1.5
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 31822; Mature: 31822

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: ACT_SITE 42-42

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPEYVRKKLTLDFGQVTDYIVRRIREYIEESKKEGGIIGLSGGIDSSVTTILLSRATNN
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCC
FYILLMPTSSTPQKDIEDAMKIIKIVNGENKYSYINIDEIINEFSSIVNISDKIVIGNIK
EEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCEEEEECHH
ARVRMTLLYAFAQKMNYLVIGTGDKSEIMLGYFTKYGDGGVDVLPIGDLYKTQVRMLGNY
HHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEEECCCCCEEEEECCHHHHHHHHHHHHH
LGVPEEIVKKPPSPALWEGQTAEGEIGLDYETIDSILYLKFEEMREPEEIAEMTKTSYDK
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEHHHHCCHHHHHHHHHHHHHH
VIKIINMVKTSQHKRLPPEIFRLSGRAINSDWRYPRQWG
HHHHHHHHHHHHCCCCCHHHHHHCCCEECCCCCCCCCCC
>Mature Secondary Structure
MMPEYVRKKLTLDFGQVTDYIVRRIREYIEESKKEGGIIGLSGGIDSSVTTILLSRATNN
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCC
FYILLMPTSSTPQKDIEDAMKIIKIVNGENKYSYINIDEIINEFSSIVNISDKIVIGNIK
EEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCEEEEECHH
ARVRMTLLYAFAQKMNYLVIGTGDKSEIMLGYFTKYGDGGVDVLPIGDLYKTQVRMLGNY
HHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEEECCCCCEEEEECCHHHHHHHHHHHHH
LGVPEEIVKKPPSPALWEGQTAEGEIGLDYETIDSILYLKFEEMREPEEIAEMTKTSYDK
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEHHHHCCHHHHHHHHHHHHHH
VIKIINMVKTSQHKRLPPEIFRLSGRAINSDWRYPRQWG
HHHHHHHHHHHHCCCCCHHHHHHCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479