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The map label for this gene is nadE2 [H]

Identifier: 15922485

GI number: 15922485

Start: 2156802

End: 2157611

Strand: Direct

Name: nadE2 [H]

Synonym: ST2160

Alternate gene names: 15922485

Gene position: 2156802-2157611 (Clockwise)

Preceding gene: 15922484

Following gene: 15922486

Centisome position: 80.04

GC content: 30.86

Gene sequence:

>810_bases
ATGGGGTTAAAAGTAGAATTAGCTCAAATAAGACCAAAACTTGGAGATGTAAAATATAATTTAGAGAAACATCAAGAAAT
AATATCTTCATCTTCTGCGGATTGTATAATTTTCCCAGAGCTTTCCTTAACTGGATATATATTAAGGGATTTAGTGTACG
AAGTGTATAATGAAAGTGAGAAAGCAATAGAAAAATTATCAGAAGAAAATAAATGTATAATAGCTGGACTAGTTAAAGAA
ATAAGACCTGGCATACTTAGAAATACGGCTGCTATAATAATTAATCATCAAATTAATTATATATATAAATTTTATTTACC
TACATATGGTTTATTTGAAGAAAGAAGATATTTTCAACCTGGAGATCCAAAAAGAGATCTAAAAATATTTGAGTACAAAG
GAGTAAAATTTGGAGTAATTATCTGTGAAGATGCATGGCATTATGAACCAATAGAGGCACTCGCTTTACTAGGTGCTGAT
AGTATATTTATTCCAGCAGCATCACCAATGAGAAGACTAAGTACTAGGCTAGGTATACAAGATAATTGGGAAGCCTTACT
AAAGGCTCATTCTATAATTAATGGTATTTGGACCATTTTCGTAAATAATGTAGGAAGTCAGGAGGAAGAATTCTTTTGGG
GAGGATCTATGGTTGTATCTCCTAATGGAGAGGTAATAAATAGGGCCAAATTATTTGAAGAAGATATAATAATTACTGAA
ATAAATCTCGATGAAGTTAGGAAAAATAGATTTTTCAGTAGTTTCAGAGAGCATAATAGAGATTTTCACGATGTATTAAG
GAGCCTCTAA

Upstream 100 bases:

>100_bases
TTATAAATATGGTGAAAACATCACAACATAAAAGACTCCCACCAGAGATTTTCAGATTAAGTGGAAGGGCAATTAACTCT
GACTGGAGGTATCCAAGGCA

Downstream 100 bases:

>100_bases
ATTTATTTCCTCTTTTATAAAACTATATTTTAGTTATGATATCTCAGAAAGTGAACATCATTGAGATGTTACTCCTCATA
CTACTTATATTACTTTTGCT

Product: glutamine-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MGLKVELAQIRPKLGDVKYNLEKHQEIISSSSADCIIFPELSLTGYILRDLVYEVYNESEKAIEKLSEENKCIIAGLVKE
IRPGILRNTAAIIINHQINYIYKFYLPTYGLFEERRYFQPGDPKRDLKIFEYKGVKFGVIICEDAWHYEPIEALALLGAD
SIFIPAASPMRRLSTRLGIQDNWEALLKAHSIINGIWTIFVNNVGSQEEEFFWGGSMVVSPNGEVINRAKLFEEDIIITE
INLDEVRKNRFFSSFREHNRDFHDVLRSL

Sequences:

>Translated_269_residues
MGLKVELAQIRPKLGDVKYNLEKHQEIISSSSADCIIFPELSLTGYILRDLVYEVYNESEKAIEKLSEENKCIIAGLVKE
IRPGILRNTAAIIINHQINYIYKFYLPTYGLFEERRYFQPGDPKRDLKIFEYKGVKFGVIICEDAWHYEPIEALALLGAD
SIFIPAASPMRRLSTRLGIQDNWEALLKAHSIINGIWTIFVNNVGSQEEEFFWGGSMVVSPNGEVINRAKLFEEDIIITE
INLDEVRKNRFFSSFREHNRDFHDVLRSL
>Mature_268_residues
GLKVELAQIRPKLGDVKYNLEKHQEIISSSSADCIIFPELSLTGYILRDLVYEVYNESEKAIEKLSEENKCIIAGLVKEI
RPGILRNTAAIIINHQINYIYKFYLPTYGLFEERRYFQPGDPKRDLKIFEYKGVKFGVIICEDAWHYEPIEALALLGADS
IFIPAASPMRRLSTRLGIQDNWEALLKAHSIINGIWTIFVNNVGSQEEEFFWGGSMVVSPNGEVINRAKLFEEDIIITEI
NLDEVRKNRFFSSFREHNRDFHDVLRSL

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 31064; Mature: 30933

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKVELAQIRPKLGDVKYNLEKHQEIISSSSADCIIFPELSLTGYILRDLVYEVYNESE
CCCEEEHHHHCCCCCCEEECHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCHH
KAIEKLSEENKCIIAGLVKEIRPGILRNTAAIIINHQINYIYKFYLPTYGLFEERRYFQP
HHHHHHCCCCCEEHHHHHHHHCCCHHCCCEEEEEECCEEEEEEEECCCCCHHHHHCCCCC
GDPKRDLKIFEYKGVKFGVIICEDAWHYEPIEALALLGADSIFIPAASPMRRLSTRLGIQ
CCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHCCCC
DNWEALLKAHSIINGIWTIFVNNVGSQEEEFFWGGSMVVSPNGEVINRAKLFEEDIIITE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEECCCCCHHHHHHHHHCCEEEEE
INLDEVRKNRFFSSFREHNRDFHDVLRSL
CCHHHHHHHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
GLKVELAQIRPKLGDVKYNLEKHQEIISSSSADCIIFPELSLTGYILRDLVYEVYNESE
CCEEEHHHHCCCCCCEEECHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCHH
KAIEKLSEENKCIIAGLVKEIRPGILRNTAAIIINHQINYIYKFYLPTYGLFEERRYFQP
HHHHHHCCCCCEEHHHHHHHHCCCHHCCCEEEEEECCEEEEEEEECCCCCHHHHHCCCCC
GDPKRDLKIFEYKGVKFGVIICEDAWHYEPIEALALLGADSIFIPAASPMRRLSTRLGIQ
CCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHCCCC
DNWEALLKAHSIINGIWTIFVNNVGSQEEEFFWGGSMVVSPNGEVINRAKLFEEDIIITE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEECCCCCHHHHHHHHHCCEEEEE
INLDEVRKNRFFSSFREHNRDFHDVLRSL
CCHHHHHHHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]