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The map label for this gene is mutB [H]

Identifier: 15922422

GI number: 15922422

Start: 2105634

End: 2106056

Strand: Direct

Name: mutB [H]

Synonym: ST2096

Alternate gene names: 15922422

Gene position: 2105634-2106056 (Clockwise)

Preceding gene: 15922420

Following gene: 15922423

Centisome position: 78.14

GC content: 37.83

Gene sequence:

>423_bases
ATGAGCCAGAAAAGGATTAAGGTTTTAGTTGCAAAATTAGGTCTGGACGGACATGATAGAGGTGCTAAAGTAATAGCAAG
AGCATTAAAGGATGCGGGAATGGAAGTCGTTTATACTGGCTTAAGGCAAACCCCAGAGCAGATAGTAAAATCTGCAATAC
AAGAGGACGTAGATGTTATTGGTATTAGTATCTTAAGTGGTGCTCACTTAGAATTGGTACCTTATGTTGTAAATCTTATG
CGTGAAAAAGGATTAAATGATGTTGTATTAGTTGTCGGTGGAGTTATACCACCTCAAGATATACCAAAACTTAAGGAGAT
GGGTGTTGATGAAGTCTTTTTACCCGGTTCTAGCCTAAAAGAGGTAGTTGAGAAAATAACAAAAGCTGTAGAGACTAAAA
GGGGCATAAAGATTGCTTCTTGA

Upstream 100 bases:

>100_bases
AATGGATAATTATATAAAATTTACCGTCTTATTAATCTATATTTATATTCATAATTAGTATCATACTAAAATTTAAGCAA
TTTAAACTTACTATATACTG

Downstream 100 bases:

>100_bases
AAAAGCCTTAGCTGGTGAGGAGTTAGCAATCTCTAGGCTTTTAACAAAAATAGAGTACTTTACTCAAGAGGGGTTAGAAA
GCTTACAAGAGTTAATTAAA

Product: methylmalonyl-CoA mutase subunit alpha

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MSQKRIKVLVAKLGLDGHDRGAKVIARALKDAGMEVVYTGLRQTPEQIVKSAIQEDVDVIGISILSGAHLELVPYVVNLM
REKGLNDVVLVVGGVIPPQDIPKLKEMGVDEVFLPGSSLKEVVEKITKAVETKRGIKIAS

Sequences:

>Translated_140_residues
MSQKRIKVLVAKLGLDGHDRGAKVIARALKDAGMEVVYTGLRQTPEQIVKSAIQEDVDVIGISILSGAHLELVPYVVNLM
REKGLNDVVLVVGGVIPPQDIPKLKEMGVDEVFLPGSSLKEVVEKITKAVETKRGIKIAS
>Mature_139_residues
SQKRIKVLVAKLGLDGHDRGAKVIARALKDAGMEVVYTGLRQTPEQIVKSAIQEDVDVIGISILSGAHLELVPYVVNLMR
EKGLNDVVLVVGGVIPPQDIPKLKEMGVDEVFLPGSSLKEVVEKITKAVETKRGIKIAS

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. This conversion most likely represents an important source of building blocks for polyketide antibiotic biosynthes

COG id: COG2185

COG function: function code I; Methylmalonyl-CoA mutase, C-terminal domain/subunit (cobalamin-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=135, Percent_Identity=42.962962962963, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1789284, Length=123, Percent_Identity=43.0894308943089, Blast_Score=111, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25144500, Length=130, Percent_Identity=42.3076923076923, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 15105; Mature: 14974

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKRIKVLVAKLGLDGHDRGAKVIARALKDAGMEVVYTGLRQTPEQIVKSAIQEDVDVI
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
GISILSGAHLELVPYVVNLMREKGLNDVVLVVGGVIPPQDIPKLKEMGVDEVFLPGSSLK
HHHHHCCCCHHHHHHHHHHHHHCCCCCEEHEECCCCCCCCCHHHHHCCCCEEECCCHHHH
EVVEKITKAVETKRGIKIAS
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQKRIKVLVAKLGLDGHDRGAKVIARALKDAGMEVVYTGLRQTPEQIVKSAIQEDVDVI
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
GISILSGAHLELVPYVVNLMREKGLNDVVLVVGGVIPPQDIPKLKEMGVDEVFLPGSSLK
HHHHHCCCCHHHHHHHHHHHHHCCCCCEEHEECCCCCCCCCHHHHHCCCCEEECCCHHHH
EVVEKITKAVETKRGIKIAS
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8099072 [H]