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The map label for this gene is gcvH3

Identifier: 15922210

GI number: 15922210

Start: 1900814

End: 1901224

Strand: Direct

Name: gcvH3

Synonym: ST1894

Alternate gene names: 15922210

Gene position: 1900814-1901224 (Clockwise)

Preceding gene: 15922208

Following gene: 15922211

Centisome position: 70.54

GC content: 31.14

Gene sequence:

>411_bases
ATGCAAGTAGAAGGTTTTGAATTTCCAGATGAGCTTTATTACTACCCAGAAGAACATGTATGGGTAAAAATTGAAGGAGA
CATGGTTACTGTAGGTATAACATCACTTGGTCAATATATGGCTGGTAAAATATTTCAAGTTACAACTAAAAATAAAGGGG
AAAAAGTAACTCCTAAAAGTGTAATTTTCACATTAGAATCTGCTAAGTGGATTGGAAAATTTAGATTACCCGTAGAAGGA
GAAATAGTTGACATTAATGATGAAGTTGTAAAAAATCCAACTCTGATTAACGAAAAACCATATGATGCATGGATAGTTAA
AATAAAAGGAGACATAAAGAAGGAAAAATTACTCCCAGTATCTGAGGCTGTTAAAATATTTGAAAATGACGCTAAAAGAG
TAATTAGATAA

Upstream 100 bases:

>100_bases
ATCGGTTAGGTCTATCTTTAGGATTACTCCATCGATCCAAACGGCGTATTTCATTAATTAATAATAAAAGTTAATAAGAT
TAAATTTTATACTTTAACTC

Downstream 100 bases:

>100_bases
TTAATTTAAATTTCTTTTTCGAGCAAAACATTTTTATTATATTTCTTAGATATAGTAGAAGAGAGGGTTAAATATGGCAA
ATGTCTCAAATTGTGAAATT

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MQVEGFEFPDELYYYPEEHVWVKIEGDMVTVGITSLGQYMAGKIFQVTTKNKGEKVTPKSVIFTLESAKWIGKFRLPVEG
EIVDINDEVVKNPTLINEKPYDAWIVKIKGDIKKEKLLPVSEAVKIFENDAKRVIR

Sequences:

>Translated_136_residues
MQVEGFEFPDELYYYPEEHVWVKIEGDMVTVGITSLGQYMAGKIFQVTTKNKGEKVTPKSVIFTLESAKWIGKFRLPVEG
EIVDINDEVVKNPTLINEKPYDAWIVKIKGDIKKEKLLPVSEAVKIFENDAKRVIR
>Mature_136_residues
MQVEGFEFPDELYYYPEEHVWVKIEGDMVTVGITSLGQYMAGKIFQVTTKNKGEKVTPKSVIFTLESAKWIGKFRLPVEG
EIVDINDEVVKNPTLINEKPYDAWIVKIKGDIKKEKLLPVSEAVKIFENDAKRVIR

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Escherichia coli, GI1789271, Length=103, Percent_Identity=36.8932038834951, Blast_Score=63, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH3_SULTO (Q96ZD7)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377879.1
- ProteinModelPortal:   Q96ZD7
- SMR:   Q96ZD7
- GeneID:   1459953
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1894
- NMPDR:   fig|273063.1.peg.2047
- HOGENOM:   HBG693789
- OMA:   VESAKWI
- ProtClustDB:   CLSK803006
- BioCyc:   STOK273063:ST1894-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR011053
- PANTHER:   PTHR11715

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 15605; Mature: 15605

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVEGFEFPDELYYYPEEHVWVKIEGDMVTVGITSLGQYMAGKIFQVTTKNKGEKVTPKS
CCCCCCCCCHHHEECCCCEEEEEECCCEEEEEHHHHHHHHCCEEEEEEECCCCCEECCCC
VIFTLESAKWIGKFRLPVEGEIVDINDEVVKNPTLINEKPYDAWIVKIKGDIKKEKLLPV
EEEEECCCCEEEEEECCCCCEEEECCHHHHCCCEECCCCCCCEEEEEEECCCCCCCCCCH
SEAVKIFENDAKRVIR
HHHHHHHHCCHHHHCC
>Mature Secondary Structure
MQVEGFEFPDELYYYPEEHVWVKIEGDMVTVGITSLGQYMAGKIFQVTTKNKGEKVTPKS
CCCCCCCCCHHHEECCCCEEEEEECCCEEEEEHHHHHHHHCCEEEEEEECCCCCEECCCC
VIFTLESAKWIGKFRLPVEGEIVDINDEVVKNPTLINEKPYDAWIVKIKGDIKKEKLLPV
EEEEECCCCEEEEEECCCCCEEEECCHHHHCCCEECCCCCCCEEEEEEECCCCCCCCCCH
SEAVKIFENDAKRVIR
HHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479