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The map label for this gene is gcvH2

Identifier: 15922211

GI number: 15922211

Start: 1901298

End: 1901744

Strand: Direct

Name: gcvH2

Synonym: ST1895

Alternate gene names: 15922211

Gene position: 1901298-1901744 (Clockwise)

Preceding gene: 15922210

Following gene: 15922212

Centisome position: 70.56

GC content: 38.03

Gene sequence:

>447_bases
ATGGCAAATGTCTCAAATTGTGAAATTCCAGAAAATTTATTATATTTCATAGAAGGTAAGAATACTGTATGGGCAAAACA
AGAAGCCCCAGATGTCATAGTAGTAGGAATAACTGATATTGCACAAACAATGGCTGGTAAGGTAGTTAAGGTTAGATTAA
AGAAAAAGGGAACTAAAGTTGAAAGGGGTAAACCAGTAGCTACAATGGAGAGCGGAAAGTGGGCTGGACCAGTCCCAGCA
CCAGCAAGTGGAGAAATTATTGATGTAAATCCAGATGTCGAGAAAAACCCGGTTTTAGTTAATCAAGATCCTTACGGAAA
GGGATGGTTAGTAAAGATGAAAGTTAACAATCCAGAAGAGCTTAAGCAATTGGTTACTGGAAGCGCAGCAGTATCTAAAT
TAACTGAACTGATTAATAGTGAGAAACTACAGTGCAAGAGGCTATGA

Upstream 100 bases:

>100_bases
AATGACGCTAAAAGAGTAATTAGATAATTAATTTAAATTTCTTTTTCGAGCAAAACATTTTTATTATATTTCTTAGATAT
AGTAGAAGAGAGGGTTAAAT

Downstream 100 bases:

>100_bases
AATGTCCTGGAGGTTTATTTCCCTCCCTCCTCAAGACGGCTATCATATGGTAACATCTTTTGTAGCTGTAGCTGATTATG
TCTCAAGAGGAGGGAAAAAC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MANVSNCEIPENLLYFIEGKNTVWAKQEAPDVIVVGITDIAQTMAGKVVKVRLKKKGTKVERGKPVATMESGKWAGPVPA
PASGEIIDVNPDVEKNPVLVNQDPYGKGWLVKMKVNNPEELKQLVTGSAAVSKLTELINSEKLQCKRL

Sequences:

>Translated_148_residues
MANVSNCEIPENLLYFIEGKNTVWAKQEAPDVIVVGITDIAQTMAGKVVKVRLKKKGTKVERGKPVATMESGKWAGPVPA
PASGEIIDVNPDVEKNPVLVNQDPYGKGWLVKMKVNNPEELKQLVTGSAAVSKLTELINSEKLQCKRL
>Mature_147_residues
ANVSNCEIPENLLYFIEGKNTVWAKQEAPDVIVVGITDIAQTMAGKVVKVRLKKKGTKVERGKPVATMESGKWAGPVPAP
ASGEIIDVNPDVEKNPVLVNQDPYGKGWLVKMKVNNPEELKQLVTGSAAVSKLTELINSEKLQCKRL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=95, Percent_Identity=40, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI89057342, Length=95, Percent_Identity=38.9473684210526, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1789271, Length=121, Percent_Identity=38.8429752066116, Blast_Score=85, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17551294, Length=93, Percent_Identity=37.6344086021505, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507493, Length=94, Percent_Identity=39.3617021276596, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319272, Length=124, Percent_Identity=33.8709677419355, Blast_Score=72, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17865652, Length=93, Percent_Identity=41.9354838709677, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH2_SULTO (Q96ZD6)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377880.1
- ProteinModelPortal:   Q96ZD6
- SMR:   Q96ZD6
- GeneID:   1459954
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1895
- NMPDR:   fig|273063.1.peg.2048
- HOGENOM:   HBG693789
- OMA:   IPENLLY
- ProtClustDB:   PRK13380
- BioCyc:   STOK273063:ST1895-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR011053
- PANTHER:   PTHR11715

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 16108; Mature: 15977

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVSNCEIPENLLYFIEGKNTVWAKQEAPDVIVVGITDIAQTMAGKVVKVRLKKKGTKV
CCCCCCCCCCCCEEEEEECCCEEEECCCCCCEEEEEHHHHHHHHHCCEEEEEECCCCCCC
ERGKPVATMESGKWAGPVPAPASGEIIDVNPDVEKNPVLVNQDPYGKGWLVKMKVNNPEE
CCCCCEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCEEECCCCCCCCEEEEEECCCHHH
LKQLVTGSAAVSKLTELINSEKLQCKRL
HHHHHCCHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure 
ANVSNCEIPENLLYFIEGKNTVWAKQEAPDVIVVGITDIAQTMAGKVVKVRLKKKGTKV
CCCCCCCCCCCEEEEEECCCEEEECCCCCCEEEEEHHHHHHHHHCCEEEEEECCCCCCC
ERGKPVATMESGKWAGPVPAPASGEIIDVNPDVEKNPVLVNQDPYGKGWLVKMKVNNPEE
CCCCCEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCEEECCCCCCCCEEEEEECCCHHH
LKQLVTGSAAVSKLTELINSEKLQCKRL
HHHHHCCHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479