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The map label for this gene is cbiF [H]

Identifier: 15922129

GI number: 15922129

Start: 1832222

End: 1833058

Strand: Reverse

Name: cbiF [H]

Synonym: ST1816

Alternate gene names: 15922129

Gene position: 1833058-1832222 (Counterclockwise)

Preceding gene: 15922130

Following gene: 15922128

Centisome position: 68.02

GC content: 36.44

Gene sequence:

>837_bases
GTGAACACTGCTGAGCTAACACTAGAGGAAATAACTGATCTTATGATAAAAAAAGCAAAGGAAGGAAAATTAGTTGCTAG
ACTGAAGTCCGGTGATTTTTCTATATATGGTGCTTTAATGGAAGAACTGTGGGCATTACAAGACGCTGGGATAGATTATG
AACTAGTCCCCGGAATTACTGCCGCAATTGCTGCCGCTTCAGTTTTAGGTATTGAATTAACATTGCCTAAGGTATCTCAA
ACTGTAATAATAACTAGGGCTTCAGCTTCAGTTCCAATGGAAGGTTCTATAAAGGACTTTGCCCCTCTACTAAATAAGGG
GGCTTCACTTGTCATTTATACTGGGGTTCATGTAATCGATAAGGTTGTTGAAGAAATAAGGGAAGCTGGGATATCTGATG
ATATTCCAGTTGCTGTAGTATATAAGGCTACATGGTGGGGTGAACAAAAGATAATTTTAGGTGAATTAAGGGACATTGCT
GAAAAAGTAAAGAAGGAGAAAATAACCAGGGACGCAGTAATTATCGTTGGTAAGATTGTTAGACCAGAAAAATATAAGGG
TTTAGTTAGATCAAGTACCTACGATCCTAAACATTACACTAGCTTTAGAACCGCTGAGGTGACTGATAAGTTAAAGTACT
TTGAACTTAGATATATGACGGATAAAATATTTGGAGAGGAATACTTAAAAGCACTAATTGAGGCCTATACCACTTCTTCC
AAGGAGGAGAAAGAAGTTATAGTGGAGAAAATTAAGAAAGTACTTAATGAAGCAAAAATAAATCCTAAGTTGAAGGAAGA
AGCTGAGAAAATTATAAAATTACACCTGGATTTTTGA

Upstream 100 bases:

>100_bases
CAAGGCAAAAAATTACATTGAAGACGCAGATGTAATTTTGTATGCAGGATCATTGATAAACCCAGAAATATTAAAGTGGG
CTAGGAAAGATGCTGAAATT

Downstream 100 bases:

>100_bases
AATCATTTTATGAATAAGTGAAGTTATAATATAATTTTATAGCTGGAATAATATTCCAAAATCATACGGTAAATGGAATA
ATTTCTTTGCTAATAGTTTT

Product: precorrin-4 c11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNTAELTLEEITDLMIKKAKEGKLVARLKSGDFSIYGALMEELWALQDAGIDYELVPGITAAIAAASVLGIELTLPKVSQ
TVIITRASASVPMEGSIKDFAPLLNKGASLVIYTGVHVIDKVVEEIREAGISDDIPVAVVYKATWWGEQKIILGELRDIA
EKVKKEKITRDAVIIVGKIVRPEKYKGLVRSSTYDPKHYTSFRTAEVTDKLKYFELRYMTDKIFGEEYLKALIEAYTTSS
KEEKEVIVEKIKKVLNEAKINPKLKEEAEKIIKLHLDF

Sequences:

>Translated_278_residues
MNTAELTLEEITDLMIKKAKEGKLVARLKSGDFSIYGALMEELWALQDAGIDYELVPGITAAIAAASVLGIELTLPKVSQ
TVIITRASASVPMEGSIKDFAPLLNKGASLVIYTGVHVIDKVVEEIREAGISDDIPVAVVYKATWWGEQKIILGELRDIA
EKVKKEKITRDAVIIVGKIVRPEKYKGLVRSSTYDPKHYTSFRTAEVTDKLKYFELRYMTDKIFGEEYLKALIEAYTTSS
KEEKEVIVEKIKKVLNEAKINPKLKEEAEKIIKLHLDF
>Mature_278_residues
MNTAELTLEEITDLMIKKAKEGKLVARLKSGDFSIYGALMEELWALQDAGIDYELVPGITAAIAAASVLGIELTLPKVSQ
TVIITRASASVPMEGSIKDFAPLLNKGASLVIYTGVHVIDKVVEEIREAGISDDIPVAVVYKATWWGEQKIILGELRDIA
EKVKKEKITRDAVIIVGKIVRPEKYKGLVRSSTYDPKHYTSFRTAEVTDKLKYFELRYMTDKIFGEEYLKALIEAYTTSS
KEEKEVIVEKIKKVLNEAKINPKLKEEAEKIIKLHLDF

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=172, Percent_Identity=33.1395348837209, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 31135; Mature: 31135

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAELTLEEITDLMIKKAKEGKLVARLKSGDFSIYGALMEELWALQDAGIDYELVPGIT
CCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEECCCHH
AAIAAASVLGIELTLPKVSQTVIITRASASVPMEGSIKDFAPLLNKGASLVIYTGVHVID
HHHHHHHHHEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECCHHHHH
KVVEEIREAGISDDIPVAVVYKATWWGEQKIILGELRDIAEKVKKEKITRDAVIIVGKIV
HHHHHHHHCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECC
RPEKYKGLVRSSTYDPKHYTSFRTAEVTDKLKYFELRYMTDKIFGEEYLKALIEAYTTSS
CCHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KEEKEVIVEKIKKVLNEAKINPKLKEEAEKIIKLHLDF
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTAELTLEEITDLMIKKAKEGKLVARLKSGDFSIYGALMEELWALQDAGIDYELVPGIT
CCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEECCCHH
AAIAAASVLGIELTLPKVSQTVIITRASASVPMEGSIKDFAPLLNKGASLVIYTGVHVID
HHHHHHHHHEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECCHHHHH
KVVEEIREAGISDDIPVAVVYKATWWGEQKIILGELRDIAEKVKKEKITRDAVIIVGKIV
HHHHHHHHCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECC
RPEKYKGLVRSSTYDPKHYTSFRTAEVTDKLKYFELRYMTDKIFGEEYLKALIEAYTTSS
CCHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KEEKEVIVEKIKKVLNEAKINPKLKEEAEKIIKLHLDF
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]