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The map label for this gene is purD [H]

Identifier: 15921792

GI number: 15921792

Start: 1494059

End: 1495492

Strand: Direct

Name: purD [H]

Synonym: ST1498

Alternate gene names: 15921792

Gene position: 1494059-1495492 (Clockwise)

Preceding gene: 15921791

Following gene: 15921793

Centisome position: 55.44

GC content: 34.73

Gene sequence:

>1434_bases
ATGAAAGTTTTGTTAATCGGAGATGGAGCAAGAGAAAACGCTCTAGCAGAAGCATTAGCTAACTCTCCTAAAGGTTATAA
GATTCATGCAATTTCTTCATACACAAATCCTGGAATAAAAGAAGCTGTAGATAAAACTAATGGAAAATATTTTAAAGGCA
ATATTAACTCTGTTGAATTTGTTAAAGAGATAGTAAAAGAGGTAAATCCAGATTTTGGTGTTATAGGTCCTGAAGATCCA
TTATTCCATGGTGTTGCAAACGTCTTCAGAGAAGAAGGCATTCCAGTAGTTGGTCCTGATAAAGAATGTGCGATGATAGA
GAAATCTAAAGTTTGGATGAGAGAACTAATGTGGAAATATAACATTCCCGGTAGGTTAAGGTTTAAGGCCTTTGAAGATC
TAAGAGATGCTATTAATTTTATACTAGAATATGGTGGTTCTATCGCTGTAAAACCATCAGAGCAAGTTGGAGGAAAAGGA
GTTAAAGTGATTGCTGATTTGCAAGCCTACCTTTCCAACGAGAAAAGAAATGCACTAACAAAGGGAATCAATGGAATTGC
TAGTTATGTTAATGATAAGGTAAAGATTATAATTGAGGAGAAGGTAGACGGACCAGAATATACATTACATGTCCTAACTG
ATGGTTATACGCAAATACCTATGCCTCTAGCACAAGATTATAAGCATGCATATGAAGACGGAATTGGTCCAGAGACTGGT
GGAATGGGTTCTATATCTGGTCCCAAAGAAGGTTTGCCATTTATTAATGAAGAAGAATATGAGAAATCATTTGAGATTGT
AAAGCTTACTGCTGAAGCTATTAAAAAAGAAACTGGAAAGGATTATGTAGGGATTCTCTCTGGGCAAATGATGCTTACTG
GTATTTGGGGACCTACGATAATTGAATATTATTCTAGGTTTGGAGATCCAGAAGCTTCTGCTATAATACCGAGAATAGAG
AGTGATTTTGGCGAAGTTCTAGAACTATTAGCGACTAGAAGATTAAGTAAAGCCAAAATAAAGGTTAATCCAACGCCATC
AGTAGTTAGAGCTATAGCACCTTTAGGATATCCCTTAAATAAGACTATGGCATCGAATCATATAATAAATATAGATGTAC
AGAAGATAAGAGAGCTAGGTTGTAAAATATACTTTGGTTCAGTCTCTCTTGAAGGAATGCAATTAGTAACTAAAGGTTCA
AGAGCATTAGAATTAGTAGTTATTAATGATTTTAAGGAAGCTTCAGATAAATTAGATAAATGCATAAATTATATTTCTTC
TGATACAAAATTAATATTCAGGCATGATATTGGAAGAACGATTGAAGATCAAGTTGAAAAAGCTGAGATAGTAAGATACA
CGTATAAGTCAAGAGAGAAAAAAGGCCTTTTTGGAGTATCAGCAGATTGGTCTCCAAATGGTGGTTTATGGTGA

Upstream 100 bases:

>100_bases
GATTCCATTTACTGGTTAAGTCTTGAAGGTCTTTATAAAGTTCTAGGTAAGAGCATATGTGTTGGTTGTATGACTAGAAA
CTATCCTAAGGTGATATAAG

Downstream 100 bases:

>100_bases
GTGAATATAAAAAGGCTGGAGTTGATTTAAACAAATTAAAGGAGTATCATACTCTAGCTTTAAACACTTTTCAAAATTCT
GGAGTATTAAAGATTGGACA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKVLLIGDGARENALAEALANSPKGYKIHAISSYTNPGIKEAVDKTNGKYFKGNINSVEFVKEIVKEVNPDFGVIGPEDP
LFHGVANVFREEGIPVVGPDKECAMIEKSKVWMRELMWKYNIPGRLRFKAFEDLRDAINFILEYGGSIAVKPSEQVGGKG
VKVIADLQAYLSNEKRNALTKGINGIASYVNDKVKIIIEEKVDGPEYTLHVLTDGYTQIPMPLAQDYKHAYEDGIGPETG
GMGSISGPKEGLPFINEEEYEKSFEIVKLTAEAIKKETGKDYVGILSGQMMLTGIWGPTIIEYYSRFGDPEASAIIPRIE
SDFGEVLELLATRRLSKAKIKVNPTPSVVRAIAPLGYPLNKTMASNHIINIDVQKIRELGCKIYFGSVSLEGMQLVTKGS
RALELVVINDFKEASDKLDKCINYISSDTKLIFRHDIGRTIEDQVEKAEIVRYTYKSREKKGLFGVSADWSPNGGLW

Sequences:

>Translated_477_residues
MKVLLIGDGARENALAEALANSPKGYKIHAISSYTNPGIKEAVDKTNGKYFKGNINSVEFVKEIVKEVNPDFGVIGPEDP
LFHGVANVFREEGIPVVGPDKECAMIEKSKVWMRELMWKYNIPGRLRFKAFEDLRDAINFILEYGGSIAVKPSEQVGGKG
VKVIADLQAYLSNEKRNALTKGINGIASYVNDKVKIIIEEKVDGPEYTLHVLTDGYTQIPMPLAQDYKHAYEDGIGPETG
GMGSISGPKEGLPFINEEEYEKSFEIVKLTAEAIKKETGKDYVGILSGQMMLTGIWGPTIIEYYSRFGDPEASAIIPRIE
SDFGEVLELLATRRLSKAKIKVNPTPSVVRAIAPLGYPLNKTMASNHIINIDVQKIRELGCKIYFGSVSLEGMQLVTKGS
RALELVVINDFKEASDKLDKCINYISSDTKLIFRHDIGRTIEDQVEKAEIVRYTYKSREKKGLFGVSADWSPNGGLW
>Mature_477_residues
MKVLLIGDGARENALAEALANSPKGYKIHAISSYTNPGIKEAVDKTNGKYFKGNINSVEFVKEIVKEVNPDFGVIGPEDP
LFHGVANVFREEGIPVVGPDKECAMIEKSKVWMRELMWKYNIPGRLRFKAFEDLRDAINFILEYGGSIAVKPSEQVGGKG
VKVIADLQAYLSNEKRNALTKGINGIASYVNDKVKIIIEEKVDGPEYTLHVLTDGYTQIPMPLAQDYKHAYEDGIGPETG
GMGSISGPKEGLPFINEEEYEKSFEIVKLTAEAIKKETGKDYVGILSGQMMLTGIWGPTIIEYYSRFGDPEASAIIPRIE
SDFGEVLELLATRRLSKAKIKVNPTPSVVRAIAPLGYPLNKTMASNHIINIDVQKIRELGCKIYFGSVSLEGMQLVTKGS
RALELVVINDFKEASDKLDKCINYISSDTKLIFRHDIGRTIEDQVEKAEIVRYTYKSREKKGLFGVSADWSPNGGLW

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=440, Percent_Identity=29.5454545454545, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI209869995, Length=440, Percent_Identity=29.5454545454545, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI209869993, Length=440, Percent_Identity=29.5454545454545, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI28416899, Length=440, Percent_Identity=29.5454545454545, Blast_Score=159, Evalue=6e-39,
Organism=Escherichia coli, GI1790438, Length=446, Percent_Identity=28.0269058295964, Blast_Score=159, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17567511, Length=456, Percent_Identity=28.5087719298246, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321203, Length=456, Percent_Identity=28.0701754385965, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI62471619, Length=447, Percent_Identity=30.2013422818792, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24582400, Length=447, Percent_Identity=30.2013422818792, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 52869; Mature: 52869

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLLIGDGARENALAEALANSPKGYKIHAISSYTNPGIKEAVDKTNGKYFKGNINSVEF
CEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEECCCCHHHH
VKEIVKEVNPDFGVIGPEDPLFHGVANVFREEGIPVVGPDKECAMIEKSKVWMRELMWKY
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHC
NIPGRLRFKAFEDLRDAINFILEYGGSIAVKPSEQVGGKGVKVIADLQAYLSNEKRNALT
CCCCEEEHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCCCCHHHHHHHHHHCCHHHHHHH
KGINGIASYVNDKVKIIIEEKVDGPEYTLHVLTDGYTQIPMPLAQDYKHAYEDGIGPETG
HHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCC
GMGSISGPKEGLPFINEEEYEKSFEIVKLTAEAIKKETGKDYVGILSGQMMLTGIWGPTI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCHHH
IEYYSRFGDPEASAIIPRIESDFGEVLELLATRRLSKAKIKVNPTPSVVRAIAPLGYPLN
HHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCC
KTMASNHIINIDVQKIRELGCKIYFGSVSLEGMQLVTKGSRALELVVINDFKEASDKLDK
HHHHCCCEEEEEHHHHHHCCCEEEECCCCCCCHHHEECCCCEEEEEEECCHHHHHHHHHH
CINYISSDTKLIFRHDIGRTIEDQVEKAEIVRYTYKSREKKGLFGVSADWSPNGGLW
HHHHHCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MKVLLIGDGARENALAEALANSPKGYKIHAISSYTNPGIKEAVDKTNGKYFKGNINSVEF
CEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEECCCCHHHH
VKEIVKEVNPDFGVIGPEDPLFHGVANVFREEGIPVVGPDKECAMIEKSKVWMRELMWKY
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHC
NIPGRLRFKAFEDLRDAINFILEYGGSIAVKPSEQVGGKGVKVIADLQAYLSNEKRNALT
CCCCEEEHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCCCCHHHHHHHHHHCCHHHHHHH
KGINGIASYVNDKVKIIIEEKVDGPEYTLHVLTDGYTQIPMPLAQDYKHAYEDGIGPETG
HHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCC
GMGSISGPKEGLPFINEEEYEKSFEIVKLTAEAIKKETGKDYVGILSGQMMLTGIWGPTI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCHHH
IEYYSRFGDPEASAIIPRIESDFGEVLELLATRRLSKAKIKVNPTPSVVRAIAPLGYPLN
HHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCC
KTMASNHIINIDVQKIRELGCKIYFGSVSLEGMQLVTKGSRALELVVINDFKEASDKLDK
HHHHCCCEEEEEHHHHHHCCCEEEECCCCCCCHHHEECCCCEEEEEEECCHHHHHHHHHH
CINYISSDTKLIFRHDIGRTIEDQVEKAEIVRYTYKSREKKGLFGVSADWSPNGGLW
HHHHHCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]