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The map label for this gene is purF [H]

Identifier: 15921790

GI number: 15921790

Start: 1491514

End: 1492845

Strand: Direct

Name: purF [H]

Synonym: ST1496

Alternate gene names: 15921790

Gene position: 1491514-1492845 (Clockwise)

Preceding gene: 15921789

Following gene: 15921791

Centisome position: 55.35

GC content: 34.38

Gene sequence:

>1332_bases
ATGAAGGTTAAAGAACACTGCGGTATTGTTGGAGTTTATGCTGATAATTCTCCAATGATTTCTTACGAATCCCTAAAATT
ATTACAACATAGGGGACAAGAATCAGCAGGAATTACTTACAGAAAAGATGGGAGTTTAGTTACCATGAAAGGCTTGGGTT
TAGTTGAAGAGGCATTAGATCCTAGGCTTCTTCCTAATGCGAAACTATCAATAGGTCATGTTAGATATTCAACTACCGGT
AAAGGTTCACTTGACGAGGCTCAACCTTTAAGTAACGGTAAAATTGCTATAGCATTTAATGGTACAATAACTAATTATTT
TAAGTTCGGAACTTCTACCGATACTGAATTTATTCTTAAGGTTCTCTCGGAGGCGCCTAACATTAAAGAAGGAATTAGAA
GGTTAGTTGACTTAGCTGACGGTGCATACTCACTCGTAGTTTTAACTAATGAAGGAGAACTTATCGGCTTTAGAGATCCT
AAAGGATTCCGTCCTCTGGTCTTAGGTAAAATTAATAACGGTTATATAATTGCTTCAGAAGATTCCGTAATCAGACAATT
AGGAGGTAAGCCTTTAAGAGATGTAAAACCTGGAGAAATGATATACATTAAAGATGGTGAAATTGAAAGCGAAGTTATTT
CTAGGGATCGTGTAAGTTTTTGCTCATTTGAGTACATCTATTTTGCCAGACCCGATTCTATAATTGATGGTGTGTCTGTT
TATAACTCTAGGATAAAGCTAGGAGAAATTTTAGCTGAAAATCATGGAGTTGATGCAGATGTAGCTATACCAGTTCCAGA
ATCCTCAATTCCGATTGCAATAGGATTCTCTAGGAAATCAAAGATTCCTATGGAATACGGTTTGATTAGAACACTCGTAG
CAAAGAGGTCTTTTATCATGCCTACACAAGATAAAAGAAATGCTGTACTAGAAGAGAAATTTGGTATAGTCAAAAGTGTA
GTAGAAAATAAGAAAGTTGTTGTCATTGATGACTCAATCGTTAGAGGAAATACTATGAGAAAAATTGTAAGAATGATAAG
GGATAATGGTGCTAAGGAAGTTCATGTAAGAATAGGCTCTCCAAAAGTAAAATATCCTTGTTATATGGGTATAGATTTTC
CATTATCTAAAGAACTAATAGCTAGTGAGAAAGATGAAAAAGAAATAGCTAAGTATATTGGTGCTGATTCAGTTGAGTTC
TTAACAGTTGAAGAAATGATTAAAGCCATAGGAAGACCTGATTTATGTCATGCATGTTTTTCTGGAGTTTATCCCCTTAA
ATTTTCATATAATTTGCAAGTATTAGAATCTATATTTAAAAAGGTGAGTTAA

Upstream 100 bases:

>100_bases
AGATAACTAAGGACCAAGGTATAATAAAAATTGATAATAAAGAGATTAATGTAAATAAAGAGATTGATAATTATTATAAC
TACTTATATGAGGTGATGTC

Downstream 100 bases:

>100_bases
ATGGCTGGAATAATAGGTGTATATGCATTTGATAAAATTTGGAATATAAGTAAATTTTTATATTATGGTCTAATAGGATT
ACAGAATAGAGGATATAGTT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MKVKEHCGIVGVYADNSPMISYESLKLLQHRGQESAGITYRKDGSLVTMKGLGLVEEALDPRLLPNAKLSIGHVRYSTTG
KGSLDEAQPLSNGKIAIAFNGTITNYFKFGTSTDTEFILKVLSEAPNIKEGIRRLVDLADGAYSLVVLTNEGELIGFRDP
KGFRPLVLGKINNGYIIASEDSVIRQLGGKPLRDVKPGEMIYIKDGEIESEVISRDRVSFCSFEYIYFARPDSIIDGVSV
YNSRIKLGEILAENHGVDADVAIPVPESSIPIAIGFSRKSKIPMEYGLIRTLVAKRSFIMPTQDKRNAVLEEKFGIVKSV
VENKKVVVIDDSIVRGNTMRKIVRMIRDNGAKEVHVRIGSPKVKYPCYMGIDFPLSKELIASEKDEKEIAKYIGADSVEF
LTVEEMIKAIGRPDLCHACFSGVYPLKFSYNLQVLESIFKKVS

Sequences:

>Translated_443_residues
MKVKEHCGIVGVYADNSPMISYESLKLLQHRGQESAGITYRKDGSLVTMKGLGLVEEALDPRLLPNAKLSIGHVRYSTTG
KGSLDEAQPLSNGKIAIAFNGTITNYFKFGTSTDTEFILKVLSEAPNIKEGIRRLVDLADGAYSLVVLTNEGELIGFRDP
KGFRPLVLGKINNGYIIASEDSVIRQLGGKPLRDVKPGEMIYIKDGEIESEVISRDRVSFCSFEYIYFARPDSIIDGVSV
YNSRIKLGEILAENHGVDADVAIPVPESSIPIAIGFSRKSKIPMEYGLIRTLVAKRSFIMPTQDKRNAVLEEKFGIVKSV
VENKKVVVIDDSIVRGNTMRKIVRMIRDNGAKEVHVRIGSPKVKYPCYMGIDFPLSKELIASEKDEKEIAKYIGADSVEF
LTVEEMIKAIGRPDLCHACFSGVYPLKFSYNLQVLESIFKKVS
>Mature_443_residues
MKVKEHCGIVGVYADNSPMISYESLKLLQHRGQESAGITYRKDGSLVTMKGLGLVEEALDPRLLPNAKLSIGHVRYSTTG
KGSLDEAQPLSNGKIAIAFNGTITNYFKFGTSTDTEFILKVLSEAPNIKEGIRRLVDLADGAYSLVVLTNEGELIGFRDP
KGFRPLVLGKINNGYIIASEDSVIRQLGGKPLRDVKPGEMIYIKDGEIESEVISRDRVSFCSFEYIYFARPDSIIDGVSV
YNSRIKLGEILAENHGVDADVAIPVPESSIPIAIGFSRKSKIPMEYGLIRTLVAKRSFIMPTQDKRNAVLEEKFGIVKSV
VENKKVVVIDDSIVRGNTMRKIVRMIRDNGAKEVHVRIGSPKVKYPCYMGIDFPLSKELIASEKDEKEIAKYIGADSVEF
LTVEEMIKAIGRPDLCHACFSGVYPLKFSYNLQVLESIFKKVS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=514, Percent_Identity=33.4630350194552, Blast_Score=286, Evalue=2e-77,
Organism=Escherichia coli, GI1788651, Length=469, Percent_Identity=37.5266524520256, Blast_Score=268, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI17554892, Length=477, Percent_Identity=35.4297693920335, Blast_Score=266, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6323958, Length=449, Percent_Identity=32.739420935412, Blast_Score=222, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24659598, Length=475, Percent_Identity=35.5789473684211, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24659604, Length=457, Percent_Identity=35.8862144420131, Blast_Score=284, Evalue=9e-77,
Organism=Drosophila melanogaster, GI28573187, Length=460, Percent_Identity=35.2173913043478, Blast_Score=271, Evalue=9e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 49070; Mature: 49070

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKEHCGIVGVYADNSPMISYESLKLLQHRGQESAGITYRKDGSLVTMKGLGLVEEALD
CCCHHHCCEEEEEECCCCEEEHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCHHHHHCC
PRLLPNAKLSIGHVRYSTTGKGSLDEAQPLSNGKIAIAFNGTITNYFKFGTSTDTEFILK
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCHHHHHH
VLSEAPNIKEGIRRLVDLADGAYSLVVLTNEGELIGFRDPKGFRPLVLGKINNGYIIASE
HHHCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCEEEEEECCCEEEECC
DSVIRQLGGKPLRDVKPGEMIYIKDGEIESEVISRDRVSFCSFEYIYFARPDSIIDGVSV
CHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHH
YNSRIKLGEILAENHGVDADVAIPVPESSIPIAIGFSRKSKIPMEYGLIRTLVAKRSFIM
HHCCHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCC
PTQDKRNAVLEEKFGIVKSVVENKKVVVIDDSIVRGNTMRKIVRMIRDNGAKEVHVRIGS
CCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCEECCHHHHHHHHHHHCCCCEEEEEEECC
PKVKYPCYMGIDFPLSKELIASEKDEKEIAKYIGADSVEFLTVEEMIKAIGRPDLCHACF
CCCCCCEEECCCCCCCHHHHHCCCCHHHHHHHHCCCCEEEEEHHHHHHHHCCCHHHHHHH
SGVYPLKFSYNLQVLESIFKKVS
CCCCEEEEECCHHHHHHHHHHCC
>Mature Secondary Structure
MKVKEHCGIVGVYADNSPMISYESLKLLQHRGQESAGITYRKDGSLVTMKGLGLVEEALD
CCCHHHCCEEEEEECCCCEEEHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCHHHHHCC
PRLLPNAKLSIGHVRYSTTGKGSLDEAQPLSNGKIAIAFNGTITNYFKFGTSTDTEFILK
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCHHHHHH
VLSEAPNIKEGIRRLVDLADGAYSLVVLTNEGELIGFRDPKGFRPLVLGKINNGYIIASE
HHHCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCEEEEEECCCEEEECC
DSVIRQLGGKPLRDVKPGEMIYIKDGEIESEVISRDRVSFCSFEYIYFARPDSIIDGVSV
CHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHH
YNSRIKLGEILAENHGVDADVAIPVPESSIPIAIGFSRKSKIPMEYGLIRTLVAKRSFIM
HHCCHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCC
PTQDKRNAVLEEKFGIVKSVVENKKVVVIDDSIVRGNTMRKIVRMIRDNGAKEVHVRIGS
CCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCEECCHHHHHHHHHHHCCCCEEEEEEECC
PKVKYPCYMGIDFPLSKELIASEKDEKEIAKYIGADSVEFLTVEEMIKAIGRPDLCHACF
CCCCCCEEECCCCCCCHHHHHCCCCHHHHHHHHCCCCEEEEEHHHHHHHHCCCHHHHHHH
SGVYPLKFSYNLQVLESIFKKVS
CCCCEEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]