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The map label for this gene is pyrI [H]

Identifier: 15921772

GI number: 15921772

Start: 1478235

End: 1478726

Strand: Reverse

Name: pyrI [H]

Synonym: ST1479

Alternate gene names: 15921772

Gene position: 1478726-1478235 (Counterclockwise)

Preceding gene: 15921773

Following gene: 15921771

Centisome position: 54.87

GC content: 31.3

Gene sequence:

>492_bases
GTGAATAAAATGGAGACCGTTAATAAAAAAGAACTAATAGTAAGTAAAATTAAAAATGGCACAGTAATAGACCACATACC
GGCAGGAAGAGCTTTAGCTGTACTTAGAATATTAAAAATTGCAGAAGGTTATAGAATAGCTCTGGTTATGAACGTAGAAA
GCAAAAAGATGGGTAAGAAAGATATTGTAAAGATCGAGAATAAAGAGGTAGATGAAAAAGAAGCTAACCTAATAACGTTA
ATAGCACCTACTGCTACAATAAACATAATTAGAGATTACGAGGTAGTTGAGAAAAAGAAACTAAAGATACCAGATATTGT
TAAAGGTCTCCTAAAATGTACTAACTTAATGTGCATTACCAACAATGATGTTGAGGCAATACCAGGATTTAAGAAAATAA
GTGAAAAACCGTTAAAAATGAAGTGCGTATACTGTGATACAGTAATAGACGAAAATGAAATTCTAAAACAAATTTTGGGT
AGCAAAAGTTGA

Upstream 100 bases:

>100_bases
GAGATTAGTAGAGAAGTAGATAAGACTCCTCAAGCTAAGTATTTTGAGCAAGCTTCTTACGCTGTTCCAGTAAGAATGGC
AATCTTCCATAAGATTGTAG

Downstream 100 bases:

>100_bases
TTTATTCTGAAATTTTATACTCTAAAAAGATTAGCAATAATTTATACGAAATTGCTACTTTTTTCCCACAAAAACCTCAG
CCAGGGCAATTCGTAAGTTT

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MNKMETVNKKELIVSKIKNGTVIDHIPAGRALAVLRILKIAEGYRIALVMNVESKKMGKKDIVKIENKEVDEKEANLITL
IAPTATINIIRDYEVVEKKKLKIPDIVKGLLKCTNLMCITNNDVEAIPGFKKISEKPLKMKCVYCDTVIDENEILKQILG
SKS

Sequences:

>Translated_163_residues
MNKMETVNKKELIVSKIKNGTVIDHIPAGRALAVLRILKIAEGYRIALVMNVESKKMGKKDIVKIENKEVDEKEANLITL
IAPTATINIIRDYEVVEKKKLKIPDIVKGLLKCTNLMCITNNDVEAIPGFKKISEKPLKMKCVYCDTVIDENEILKQILG
SKS
>Mature_163_residues
MNKMETVNKKELIVSKIKNGTVIDHIPAGRALAVLRILKIAEGYRIALVMNVESKKMGKKDIVKIENKEVDEKEANLITL
IAPTATINIIRDYEVVEKKKLKIPDIVKGLLKCTNLMCITNNDVEAIPGFKKISEKPLKMKCVYCDTVIDENEILKQILG
SKS

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase [H]

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family [H]

Homologues:

Organism=Escherichia coli, GI1790692, Length=145, Percent_Identity=36.551724137931, Blast_Score=98, Evalue=3e-22,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- ProDom:   PD006194 [H]

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C [H]

EC number: 2.1.3.2

Molecular weight: Translated: 18291; Mature: 18291

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKMETVNKKELIVSKIKNGTVIDHIPAGRALAVLRILKIAEGYRIALVMNVESKKMGKK
CCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCEEEEEEEECCHHHCCCC
DIVKIENKEVDEKEANLITLIAPTATINIIRDYEVVEKKKLKIPDIVKGLLKCTNLMCIT
CEEEECCCCCCCCCCCEEEEEECCEEEEEEECHHHHHHHCCCCHHHHHHHHHHCCEEEEE
NNDVEAIPGFKKISEKPLKMKCVYCDTVIDENEILKQILGSKS
CCCCCCCCCHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNKMETVNKKELIVSKIKNGTVIDHIPAGRALAVLRILKIAEGYRIALVMNVESKKMGKK
CCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCEEEEEEEECCHHHCCCC
DIVKIENKEVDEKEANLITLIAPTATINIIRDYEVVEKKKLKIPDIVKGLLKCTNLMCIT
CEEEECCCCCCCCCCCEEEEEECCEEEEEEECHHHHHHHCCCCHHHHHHHHHHCCEEEEE
NNDVEAIPGFKKISEKPLKMKCVYCDTVIDENEILKQILGSKS
CCCCCCCCCHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]