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The map label for this gene is pyrB

Identifier: 15921773

GI number: 15921773

Start: 1478719

End: 1479615

Strand: Reverse

Name: pyrB

Synonym: ST1480

Alternate gene names: 15921773

Gene position: 1479615-1478719 (Counterclockwise)

Preceding gene: 15921777

Following gene: 15921772

Centisome position: 54.91

GC content: 32.44

Gene sequence:

>897_bases
GTGAAGGATATAATATCAGTATATGATTTTTCAAAAAGCGATTTTGATGAATTATTCGAATTGGCTGATAAATTACGAAA
TATGTCTACATATCCAAAAATATTGAAAGAGAAAACTGTAGCATTAGCATTCTTTGAACCTAGTACTAGAACGTATTTAA
GCTTTAATAAAGCTGCAATAAACCTAGGAGCATCAACTATAGGATTTTCTGGAGAGGAAGGCACTTCTATTGCAAAGGGA
GAAAATCTAGCTGATACAATTAGAATGCTAAACAATTATGCTGATATGATAGTGATTAGGCATAAATTCGATGGAGCAGC
AAAATTTGCTTCAGAAATTTCAGAAATACCCGTGATTAACGCAGGGGATGGGAAACATGAACATCCAACACAGACAGTTA
TAGATTTTTACACAATTTATAGAAATTTCAAAAACATAGACGGTTTAACATTCGGCTTAATGGGAGATCTTAGATATGCT
AGAGTAGTTAATAGCTTTTTAAGAGCACTAACAAGATTTAAGCCAAAAAAAGTTTACTTGATCTCTCCTCCACAATTATC
TGCTAGAAAAGAAATATTAGAAGAATTGAATTATCCTTACAGAGAAGTTATTGATTATAATGAGGTTATTGAAGAGATAG
ATGTGCTGTATGTTACTAGAATTCAAAAAGAGAGATTCCCAGATGAAAGTGAGTATGAAAAAGTTAAGGAAAGTTATGTG
GTAGACATGAATTTAGTATCCAAAATGAAGAAAGATGCAATAATTTTACACGCATTGCCTAGAGTGAATGAGATTAGTAG
AGAAGTAGATAAGACTCCTCAAGCTAAGTATTTTGAGCAAGCTTCTTACGCTGTTCCAGTAAGAATGGCAATCTTCCATA
AGATTGTAGGTGAATAA

Upstream 100 bases:

>100_bases
AGAAGCTTATTCTCAACTAAGGATTTAATAAAATCCATGAATTAGATTATGAAAGAAAGAGTTTAAATAATCATTGAACG
AAGATAAATAAAGGGCATTT

Downstream 100 bases:

>100_bases
AATGGAGACCGTTAATAAAAAAGAACTAATAGTAAGTAAAATTAAAAATGGCACAGTAATAGACCACATACCGGCAGGAA
GAGCTTTAGCTGTACTTAGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKDIISVYDFSKSDFDELFELADKLRNMSTYPKILKEKTVALAFFEPSTRTYLSFNKAAINLGASTIGFSGEEGTSIAKG
ENLADTIRMLNNYADMIVIRHKFDGAAKFASEISEIPVINAGDGKHEHPTQTVIDFYTIYRNFKNIDGLTFGLMGDLRYA
RVVNSFLRALTRFKPKKVYLISPPQLSARKEILEELNYPYREVIDYNEVIEEIDVLYVTRIQKERFPDESEYEKVKESYV
VDMNLVSKMKKDAIILHALPRVNEISREVDKTPQAKYFEQASYAVPVRMAIFHKIVGE

Sequences:

>Translated_298_residues
MKDIISVYDFSKSDFDELFELADKLRNMSTYPKILKEKTVALAFFEPSTRTYLSFNKAAINLGASTIGFSGEEGTSIAKG
ENLADTIRMLNNYADMIVIRHKFDGAAKFASEISEIPVINAGDGKHEHPTQTVIDFYTIYRNFKNIDGLTFGLMGDLRYA
RVVNSFLRALTRFKPKKVYLISPPQLSARKEILEELNYPYREVIDYNEVIEEIDVLYVTRIQKERFPDESEYEKVKESYV
VDMNLVSKMKKDAIILHALPRVNEISREVDKTPQAKYFEQASYAVPVRMAIFHKIVGE
>Mature_298_residues
MKDIISVYDFSKSDFDELFELADKLRNMSTYPKILKEKTVALAFFEPSTRTYLSFNKAAINLGASTIGFSGEEGTSIAKG
ENLADTIRMLNNYADMIVIRHKFDGAAKFASEISEIPVINAGDGKHEHPTQTVIDFYTIYRNFKNIDGLTFGLMGDLRYA
RVVNSFLRALTRFKPKKVYLISPPQLSARKEILEELNYPYREVIDYNEVIEEIDVLYVTRIQKERFPDESEYEKVKESYV
VDMNLVSKMKKDAIILHALPRVNEISREVDKTPQAKYFEQASYAVPVRMAIFHKIVGE

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=302, Percent_Identity=38.4105960264901, Blast_Score=219, Evalue=3e-57,
Organism=Escherichia coli, GI2367364, Length=298, Percent_Identity=43.2885906040268, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1790703, Length=173, Percent_Identity=31.7919075144509, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786469, Length=191, Percent_Identity=30.3664921465969, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=319, Percent_Identity=37.9310344827586, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6322331, Length=303, Percent_Identity=38.6138613861386, Blast_Score=215, Evalue=7e-57,
Organism=Saccharomyces cerevisiae, GI6322373, Length=327, Percent_Identity=25.6880733944954, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24642586, Length=303, Percent_Identity=39.6039603960396, Blast_Score=214, Evalue=4e-56,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_SULTO (Q970X2)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377442.1
- ProteinModelPortal:   Q970X2
- SMR:   Q970X2
- GeneID:   1459515
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1480
- NMPDR:   fig|273063.1.peg.1610
- HOGENOM:   HBG579429
- OMA:   AQYIDSH
- ProtClustDB:   PRK00856
- BioCyc:   STOK273063:ST1480-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34234; Mature: 34234

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIISVYDFSKSDFDELFELADKLRNMSTYPKILKEKTVALAFFEPSTRTYLSFNKAAI
CCCHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEECCCCCEEEEECHHHE
NLGASTIGFSGEEGTSIAKGENLADTIRMLNNYADMIVIRHKFDGAAKFASEISEIPVIN
ECCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCEEE
AGDGKHEHPTQTVIDFYTIYRNFKNIDGLTFGLMGDLRYARVVNSFLRALTRFKPKKVYL
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ISPPQLSARKEILEELNYPYREVIDYNEVIEEIDVLYVTRIQKERFPDESEYEKVKESYV
ECCCCHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VDMNLVSKMKKDAIILHALPRVNEISREVDKTPQAKYFEQASYAVPVRMAIFHKIVGE
HHHHHHHHHHHHHEEEHHCCCHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKDIISVYDFSKSDFDELFELADKLRNMSTYPKILKEKTVALAFFEPSTRTYLSFNKAAI
CCCHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEECCCCCEEEEECHHHE
NLGASTIGFSGEEGTSIAKGENLADTIRMLNNYADMIVIRHKFDGAAKFASEISEIPVIN
ECCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCEEE
AGDGKHEHPTQTVIDFYTIYRNFKNIDGLTFGLMGDLRYARVVNSFLRALTRFKPKKVYL
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ISPPQLSARKEILEELNYPYREVIDYNEVIEEIDVLYVTRIQKERFPDESEYEKVKESYV
ECCCCHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VDMNLVSKMKKDAIILHALPRVNEISREVDKTPQAKYFEQASYAVPVRMAIFHKIVGE
HHHHHHHHHHHHHEEEHHCCCHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479