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The map label for this gene is hisA

Identifier: 15921753

GI number: 15921753

Start: 1465009

End: 1465701

Strand: Direct

Name: hisA

Synonym: ST1460

Alternate gene names: 15921753

Gene position: 1465009-1465701 (Clockwise)

Preceding gene: 15921752

Following gene: 15921754

Centisome position: 54.37

GC content: 31.17

Gene sequence:

>693_bases
ATGCTAAAAGTCGTACCCAGTATTGATATTAGTGAAGGGAAGGCAGTGAAAAGGATAAGAGGTGTAAAAGGCTCTGGTTT
AATTCTCGGTAACCCCGTTAAGATAGCATATGAGATTTACGAGGAAGGTTATGATTATCTACATGTAGTTGACTTAGATT
CCGCTGAAGAGAACGGAAATAATGAAGAATATGTTAAAGATATATGTAAAATAGGTTTTAAGTGGGTTCAAGTTGGTGGA
GGGATCAGAAATGTTGAAAAAGCTGAGAGATTACTAGATTACGATTGTTCTGCTATAGTTATTTCCACATTACCAATAAA
AGATCCTAAATCTTTTATGGATATACAAAAAATAATAGGAAAAGATAAAATTTTACTTTCTGTTGACTATGATTCCTCAG
GTTATGTATTAATAAGAGGATGGAAAGAGAAATCAATAAAAGTTTTAGATTTTCTTCTTTCTTATGATTCCTTGGGTTAT
ATATTTACTTATGTAGAGAATGAAGGTACTAAGAGAGGAATTGATAACAACGTGAAAAATTATGTTATAAGAATAAAAGG
GCTAAAAGAATACGCTGGTGGAATAGGATCTATAGAAGATTTATATAAGTTAAATGAGTATGGTATTAATTATGCGATAA
TAGGTATGAGCTTTTATTCTGGCAGTTTAAGAGGGATAAAATATGTCTACTAG

Upstream 100 bases:

>100_bases
CGGTGGTTGATGAAGATCTTATACCGGAAGTAATAGCTAAAGTAAAAGCTAATGGTGCTAGGGATATTGTAGTAGTTAAT
ATAGAGAAAGTGGTGAAATA

Downstream 100 bases:

>100_bases
GAGTGTAAGAAAAATCAGAGAAACTAAAGAAACTAAAATAGAATTATATCTAGATATAGATAAGAAAGGAGAGGTAAAAG
TATCTACTCCAGTTAATTTT

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MLKVVPSIDISEGKAVKRIRGVKGSGLILGNPVKIAYEIYEEGYDYLHVVDLDSAEENGNNEEYVKDICKIGFKWVQVGG
GIRNVEKAERLLDYDCSAIVISTLPIKDPKSFMDIQKIIGKDKILLSVDYDSSGYVLIRGWKEKSIKVLDFLLSYDSLGY
IFTYVENEGTKRGIDNNVKNYVIRIKGLKEYAGGIGSIEDLYKLNEYGINYAIIGMSFYSGSLRGIKYVY

Sequences:

>Translated_230_residues
MLKVVPSIDISEGKAVKRIRGVKGSGLILGNPVKIAYEIYEEGYDYLHVVDLDSAEENGNNEEYVKDICKIGFKWVQVGG
GIRNVEKAERLLDYDCSAIVISTLPIKDPKSFMDIQKIIGKDKILLSVDYDSSGYVLIRGWKEKSIKVLDFLLSYDSLGY
IFTYVENEGTKRGIDNNVKNYVIRIKGLKEYAGGIGSIEDLYKLNEYGINYAIIGMSFYSGSLRGIKYVY
>Mature_230_residues
MLKVVPSIDISEGKAVKRIRGVKGSGLILGNPVKIAYEIYEEGYDYLHVVDLDSAEENGNNEEYVKDICKIGFKWVQVGG
GIRNVEKAERLLDYDCSAIVISTLPIKDPKSFMDIQKIIGKDKILLSVDYDSSGYVLIRGWKEKSIKVLDFLLSYDSLGY
IFTYVENEGTKRGIDNNVKNYVIRIKGLKEYAGGIGSIEDLYKLNEYGINYAIIGMSFYSGSLRGIKYVY

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=232, Percent_Identity=27.1551724137931, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_SULTO (Q970Z2)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377422.1
- ProteinModelPortal:   Q970Z2
- GeneID:   1459495
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1460
- NMPDR:   fig|273063.1.peg.1590
- HOGENOM:   HBG541613
- OMA:   GRCQLGG
- ProtClustDB:   PRK13586
- BioCyc:   STOK273063:ST1460-MONOMER
- BRENDA:   5.3.1.16
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 25878; Mature: 25878

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: ACT_SITE 9-9 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVVPSIDISEGKAVKRIRGVKGSGLILGNPVKIAYEIYEEGYDYLHVVDLDSAEENGN
CEEECCCCCCCCCHHHHHHHCCCCCCEEEECCHHEEHEEHHCCCCEEEEEECCCCCCCCC
NEEYVKDICKIGFKWVQVGGGIRNVEKAERLLDYDCSAIVISTLPIKDPKSFMDIQKIIG
CHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHC
KDKILLSVDYDSSGYVLIRGWKEKSIKVLDFLLSYDSLGYIFTYVENEGTKRGIDNNVKN
CCEEEEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCE
YVIRIKGLKEYAGGIGSIEDLYKLNEYGINYAIIGMSFYSGSLRGIKYVY
EEEEEECCHHHCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCEEECC
>Mature Secondary Structure
MLKVVPSIDISEGKAVKRIRGVKGSGLILGNPVKIAYEIYEEGYDYLHVVDLDSAEENGN
CEEECCCCCCCCCHHHHHHHCCCCCCEEEECCHHEEHEEHHCCCCEEEEEECCCCCCCCC
NEEYVKDICKIGFKWVQVGGGIRNVEKAERLLDYDCSAIVISTLPIKDPKSFMDIQKIIG
CHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHC
KDKILLSVDYDSSGYVLIRGWKEKSIKVLDFLLSYDSLGYIFTYVENEGTKRGIDNNVKN
CCEEEEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCE
YVIRIKGLKEYAGGIGSIEDLYKLNEYGINYAIIGMSFYSGSLRGIKYVY
EEEEEECCHHHCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479