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The map label for this gene is gcvH [H]

Identifier: 15921468

GI number: 15921468

Start: 1207443

End: 1207871

Strand: Reverse

Name: gcvH [H]

Synonym: ST1205

Alternate gene names: 15921468

Gene position: 1207871-1207443 (Counterclockwise)

Preceding gene: 15921469

Following gene: 15921467

Centisome position: 44.82

GC content: 32.63

Gene sequence:

>429_bases
GTGATAGTAATGTCAGAAATTATAGTCGGTGGAAAATACAAAGTATTAAAAGATAGATATTATTCAGAAACAGATGAGTG
GATATTGGTTAAAGAAAACATAGCTATTGTGGGAATAACTGATTATGCACAGAAAAAACTACGTGATATCGTAGGTGTTG
AATTACCAGAGATAGATAAAGAAGTTAAGGCTAATGATCCGGTTGCTGTTGTAGAATCCGTAAAAGCCGCTGCAGATATT
TACTCTCCATTATCTGGAAAAGTAATAGAAACAAACCAAAAACTGACTGAGTCACCAGAACTAATAAATAAAGATCCTTA
TGGTGAAGGCTGGATATTTAAACTAGAGATAAGTGACAGGAACGAAATAGAAAAATTGTTAAGTCCGGAAAAGTATAGTG
AATTAATAAAACAAAGAGAGGGATTATAA

Upstream 100 bases:

>100_bases
GGTATATTAACTCAAAATACGCTCTATTCGGCTACTCTGCCAATATAGAGATAAGAAATAGGCAAGTTGAAGCTAAATTA
CAAGACTTTCCATTTATTTA

Downstream 100 bases:

>100_bases
TGAGCCAATACGAAATTATTGAAAAATATACTCCAATTTTATCTAAACCTTCTTATCTTATAGTTGGATTACCAGACGCC
GGATTAGTTGGAGTGATAGC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MIVMSEIIVGGKYKVLKDRYYSETDEWILVKENIAIVGITDYAQKKLRDIVGVELPEIDKEVKANDPVAVVESVKAAADI
YSPLSGKVIETNQKLTESPELINKDPYGEGWIFKLEISDRNEIEKLLSPEKYSELIKQREGL

Sequences:

>Translated_142_residues
MIVMSEIIVGGKYKVLKDRYYSETDEWILVKENIAIVGITDYAQKKLRDIVGVELPEIDKEVKANDPVAVVESVKAAADI
YSPLSGKVIETNQKLTESPELINKDPYGEGWIFKLEISDRNEIEKLLSPEKYSELIKQREGL
>Mature_142_residues
MIVMSEIIVGGKYKVLKDRYYSETDEWILVKENIAIVGITDYAQKKLRDIVGVELPEIDKEVKANDPVAVVESVKAAADI
YSPLSGKVIETNQKLTESPELINKDPYGEGWIFKLEISDRNEIEKLLSPEKYSELIKQREGL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=126, Percent_Identity=41.2698412698413, Blast_Score=112, Evalue=1e-25,
Organism=Homo sapiens, GI89057342, Length=126, Percent_Identity=40.4761904761905, Blast_Score=109, Evalue=8e-25,
Organism=Escherichia coli, GI1789271, Length=120, Percent_Identity=46.6666666666667, Blast_Score=115, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17551294, Length=123, Percent_Identity=43.9024390243902, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=123, Percent_Identity=45.5284552845528, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319272, Length=116, Percent_Identity=40.5172413793103, Blast_Score=94, Evalue=7e-21,
Organism=Drosophila melanogaster, GI17865652, Length=121, Percent_Identity=42.1487603305785, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 16115; Mature: 16115

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVMSEIIVGGKYKVLKDRYYSETDEWILVKENIAIVGITDYAQKKLRDIVGVELPEIDK
CEEECCEEECCCHHHHHHHCCCCCCCEEEEECCEEEEEECHHHHHHHHHHHCCCCCCCCC
EVKANDPVAVVESVKAAADIYSPLSGKVIETNQKLTESPELINKDPYGEGWIFKLEISDR
HHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCH
NEIEKLLSPEKYSELIKQREGL
HHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIVMSEIIVGGKYKVLKDRYYSETDEWILVKENIAIVGITDYAQKKLRDIVGVELPEIDK
CEEECCEEECCCHHHHHHHCCCCCCCEEEEECCEEEEEECHHHHHHHHHHHCCCCCCCCC
EVKANDPVAVVESVKAAADIYSPLSGKVIETNQKLTESPELINKDPYGEGWIFKLEISDR
HHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCH
NEIEKLLSPEKYSELIKQREGL
HHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]