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The map label for this gene is prs

Identifier: 15921182

GI number: 15921182

Start: 920984

End: 921859

Strand: Direct

Name: prs

Synonym: ST0946

Alternate gene names: 15921182

Gene position: 920984-921859 (Clockwise)

Preceding gene: 15921173

Following gene: 15921183

Centisome position: 34.18

GC content: 33.9

Gene sequence:

>876_bases
ATGATTATTATTGGAGGAACAGCAACAAACGGGATAGATGAAAATTTATCAAAAATTATTTCAGTTCCTCTTCTGAAGGT
AGAACACAAGGTTTTTCCAGACGGAGAATCATATATTAGAATTCCACAACATATAACAAATCAAGAGATTTTAGTAGTCC
AATCATTATATCCACCACAAGATAAACATTTCGTTGAATTACTCTTAATTCTAGAAACATTAGCAGATATGAAGAATAAT
AAGATTACAGCTATAGTTCCTTATTTAGCATATTCTAGACAAGATCGAAGATTTAAGGAAGGAGAAGCATTAAGCATAAA
GACAATCCTCAATGCTATTGCTAGAGCTGGTGCTGACGTATTAATAGTAATCGAACCACATAAAGAGGAGGAATTAAGCT
ATTTTGGTAAGGAAGTTAAAATTGCCGATCCAATGCCAGAACTAGCTAAAGAAGTTAGTAAAAAGGTTGAGAAACCATTT
GTTTTGGCACCAGATAGGGGAGCTTTGGAAAGAGCTAAAAGATTAGCTGAACAACTTAATGCAGAGTATTCATATATAGA
AAAAGAAAGAGATAGAGATACAGGAGAGGTTAGAATAAAGAATTTACCAGAATTAAGGCTAAGTGGAAAAGATGTGATTA
TTGTTGATGACATAATTAGCACTGGAGGCACAATGATCCAGGCTACGAGAGCTGCTTATGAGCATGGTGCTAGGAAAGTG
ATTTCTGTAGCTGTGCATTCATTATTTTTGGATAATGCCTACGAAAAATTAATTAATAGTGGAGTTAAGGAGATTGTAAC
AACTAATACTATACCACAAGATCCATCAAAAGTAACAGTAGTTGATGTATCACCAGCTATAGCGAGAAAAATATGA

Upstream 100 bases:

>100_bases
TAATGTTGTTTTGCCTGATCCAGCTGTGCCTAGGATAAATATGAAGTACATACTATTCCCGAGTTTAATCTTAAAAACTA
CTCCCTAAATTAAATAGTTA

Downstream 100 bases:

>100_bases
AATCTTATGCTATTTTAAACCAGAATAATCTATTTTTATCCTTAGAAGAACTTAAAGCAATAATAGATTCTGATGAAGTA
GAATATTATCATGGTGTAGC

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MIIIGGTATNGIDENLSKIISVPLLKVEHKVFPDGESYIRIPQHITNQEILVVQSLYPPQDKHFVELLLILETLADMKNN
KITAIVPYLAYSRQDRRFKEGEALSIKTILNAIARAGADVLIVIEPHKEEELSYFGKEVKIADPMPELAKEVSKKVEKPF
VLAPDRGALERAKRLAEQLNAEYSYIEKERDRDTGEVRIKNLPELRLSGKDVIIVDDIISTGGTMIQATRAAYEHGARKV
ISVAVHSLFLDNAYEKLINSGVKEIVTTNTIPQDPSKVTVVDVSPAIARKI

Sequences:

>Translated_291_residues
MIIIGGTATNGIDENLSKIISVPLLKVEHKVFPDGESYIRIPQHITNQEILVVQSLYPPQDKHFVELLLILETLADMKNN
KITAIVPYLAYSRQDRRFKEGEALSIKTILNAIARAGADVLIVIEPHKEEELSYFGKEVKIADPMPELAKEVSKKVEKPF
VLAPDRGALERAKRLAEQLNAEYSYIEKERDRDTGEVRIKNLPELRLSGKDVIIVDDIISTGGTMIQATRAAYEHGARKV
ISVAVHSLFLDNAYEKLINSGVKEIVTTNTIPQDPSKVTVVDVSPAIARKI
>Mature_291_residues
MIIIGGTATNGIDENLSKIISVPLLKVEHKVFPDGESYIRIPQHITNQEILVVQSLYPPQDKHFVELLLILETLADMKNN
KITAIVPYLAYSRQDRRFKEGEALSIKTILNAIARAGADVLIVIEPHKEEELSYFGKEVKIADPMPELAKEVSKKVEKPF
VLAPDRGALERAKRLAEQLNAEYSYIEKERDRDTGEVRIKNLPELRLSGKDVIIVDDIISTGGTMIQATRAAYEHGARKV
ISVAVHSLFLDNAYEKLINSGVKEIVTTNTIPQDPSKVTVVDVSPAIARKI

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI84875539, Length=302, Percent_Identity=26.158940397351, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI4506129, Length=299, Percent_Identity=25.752508361204, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI4506127, Length=297, Percent_Identity=25.9259259259259, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI28557709, Length=297, Percent_Identity=26.5993265993266, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI194018537, Length=290, Percent_Identity=24.1379310344828, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787458, Length=280, Percent_Identity=28.9285714285714, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17554704, Length=297, Percent_Identity=26.9360269360269, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25149168, Length=297, Percent_Identity=26.9360269360269, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17554702, Length=297, Percent_Identity=26.9360269360269, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71989924, Length=297, Percent_Identity=26.9360269360269, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17570245, Length=290, Percent_Identity=24.1379310344828, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320946, Length=295, Percent_Identity=29.4915254237288, Blast_Score=145, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6319403, Length=296, Percent_Identity=28.0405405405405, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321776, Length=289, Percent_Identity=27.3356401384083, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6322667, Length=166, Percent_Identity=27.1084337349398, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355239, Length=297, Percent_Identity=26.5993265993266, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45551540, Length=320, Percent_Identity=24.6875, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS_SULTO (Q973F3)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376851.1
- ProteinModelPortal:   Q973F3
- SMR:   Q973F3
- GeneID:   1458911
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0946
- NMPDR:   fig|273063.1.peg.1019
- HOGENOM:   HBG519284
- OMA:   YARQDRT
- ProtClustDB:   CLSK802976
- BioCyc:   STOK273063:ST0946-MONOMER
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_A
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 32427; Mature: 32427

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIIGGTATNGIDENLSKIISVPLLKVEHKVFPDGESYIRIPQHITNQEILVVQSLYPPQ
CEEECCCCCCCHHHHHHHHHHHHHHHECCEECCCCCCEEECCHHCCCCEEEEEECCCCCC
DKHFVELLLILETLADMKNNKITAIVPYLAYSRQDRRFKEGEALSIKTILNAIARAGADV
HHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCCCCCEEHHHHHHHHHHCCCCE
LIVIEPHKEEELSYFGKEVKIADPMPELAKEVSKKVEKPFVLAPDRGALERAKRLAEQLN
EEEECCCCHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHC
AEYSYIEKERDRDTGEVRIKNLPELRLSGKDVIIVDDIISTGGTMIQATRAAYEHGARKV
CHHHHHHHHCCCCCCCEEECCCCCEEECCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHH
ISVAVHSLFLDNAYEKLINSGVKEIVTTNTIPQDPSKVTVVDVSPAIARKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHCC
>Mature Secondary Structure
MIIIGGTATNGIDENLSKIISVPLLKVEHKVFPDGESYIRIPQHITNQEILVVQSLYPPQ
CEEECCCCCCCHHHHHHHHHHHHHHHECCEECCCCCCEEECCHHCCCCEEEEEECCCCCC
DKHFVELLLILETLADMKNNKITAIVPYLAYSRQDRRFKEGEALSIKTILNAIARAGADV
HHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCCCCCEEHHHHHHHHHHCCCCE
LIVIEPHKEEELSYFGKEVKIADPMPELAKEVSKKVEKPFVLAPDRGALERAKRLAEQLN
EEEECCCCHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHC
AEYSYIEKERDRDTGEVRIKNLPELRLSGKDVIIVDDIISTGGTMIQATRAAYEHGARKV
CHHHHHHHHCCCCCCCEEECCCCCEEECCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHH
ISVAVHSLFLDNAYEKLINSGVKEIVTTNTIPQDPSKVTVVDVSPAIARKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479