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The map label for this gene is priA

Identifier: 15921179

GI number: 15921179

Start: 918466

End: 919437

Strand: Reverse

Name: priA

Synonym: ST0943

Alternate gene names: 15921179

Gene position: 919437-918466 (Counterclockwise)

Preceding gene: 15921180

Following gene: 15921178

Centisome position: 34.12

GC content: 32.82

Gene sequence:

>972_bases
ATGCAGATTTCTACATTGCACCAAGAGCAGAATAAGATAATTAAGATTCTTTTTTCAGAATATTATGAGAAAGCTGAGTT
AGATTTACCAAATGATATGGAACTAAGAGAATTTGCATATCAGCCCTTTGATTCTGAAACATATGTAAGACACTTATCCT
TTTCATCACCACAGGAGTTAAGGCAGTATATACTTCAAAACGTACCATTACATCTATATTACTCTTCAGCAAGATATCAA
TTACCTTCAGCAAAAGAAATGGATGAAAAAGGATGGTTAGGCTCAGATTTACTTTTCGATTTAGATGCTGATGAAATATG
TGAAGTTAAAGTAAGAAGATTTTGCCCTCAAGATGGATATGAGACTCTAGCTTCTAATTGTAACGGAGAACCTCCAATTG
AGTATGCTGAAATAACTACTGAATGCATTATGAAAGTTTTTGAAAAAGCTCTTCTTATTAGAGATATTCTTAAGGAAGAT
TTTGGACTTAATGCACGAATATTCTTTTCTGGAAATAGAGGGTTTCATCTTAGAGTTACTTGTTATGAAGACTGTGCACT
GCTTGATCCAGACGATAGGAAAGAAATAGCTGAGTATTTCACATCTCCAAAACCCCCAGTTATATATGAAGGAAATCCAG
GTTGGTCTGGAAGAATAGCAAAAGGAATAGAAGGTATTAATATTGATACTCAAGTTACTATTGACATTAGAAGACTAGTT
AGAATCCCTGGTTCATTAAATGGAAAAGCTGGATTAATGGTTGTGGAAATAAAAAATGACAAATTTGAATACGGTGAATG
GCTATCCCCATTTGATGGTGATTGTATATTTTTACCATATGTTTCGGCAGAGTTAACACTATTTAACAATAAGTATACAG
TTAAGAGAACTTATCCAATAAAAATTGATACAAGGATAGGAGTATATTTAGCATTAAAAGGATTAGGAAAGGTGAAAGCA
TATGTTAGATGA

Upstream 100 bases:

>100_bases
GTAAATACGTCAAAAATGAGAAAACCTTCAGATACTGTTGAGATAGCCTTTGGATCGCAAATACCATTGAAACTAAGATA
TAATTTACCTCAAGGGGGGT

Downstream 100 bases:

>100_bases
GATAGTATTAAATGAAACCTTAAAAGAGGAACCTACTGAAATACAGTTAAAAGATTTAAACCAGTTAATAGTGCAGCTGA
GAAAACTTAGGAGAAAATAT

Product: DNA primase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MQISTLHQEQNKIIKILFSEYYEKAELDLPNDMELREFAYQPFDSETYVRHLSFSSPQELRQYILQNVPLHLYYSSARYQ
LPSAKEMDEKGWLGSDLLFDLDADEICEVKVRRFCPQDGYETLASNCNGEPPIEYAEITTECIMKVFEKALLIRDILKED
FGLNARIFFSGNRGFHLRVTCYEDCALLDPDDRKEIAEYFTSPKPPVIYEGNPGWSGRIAKGIEGINIDTQVTIDIRRLV
RIPGSLNGKAGLMVVEIKNDKFEYGEWLSPFDGDCIFLPYVSAELTLFNNKYTVKRTYPIKIDTRIGVYLALKGLGKVKA
YVR

Sequences:

>Translated_323_residues
MQISTLHQEQNKIIKILFSEYYEKAELDLPNDMELREFAYQPFDSETYVRHLSFSSPQELRQYILQNVPLHLYYSSARYQ
LPSAKEMDEKGWLGSDLLFDLDADEICEVKVRRFCPQDGYETLASNCNGEPPIEYAEITTECIMKVFEKALLIRDILKED
FGLNARIFFSGNRGFHLRVTCYEDCALLDPDDRKEIAEYFTSPKPPVIYEGNPGWSGRIAKGIEGINIDTQVTIDIRRLV
RIPGSLNGKAGLMVVEIKNDKFEYGEWLSPFDGDCIFLPYVSAELTLFNNKYTVKRTYPIKIDTRIGVYLALKGLGKVKA
YVR
>Mature_323_residues
MQISTLHQEQNKIIKILFSEYYEKAELDLPNDMELREFAYQPFDSETYVRHLSFSSPQELRQYILQNVPLHLYYSSARYQ
LPSAKEMDEKGWLGSDLLFDLDADEICEVKVRRFCPQDGYETLASNCNGEPPIEYAEITTECIMKVFEKALLIRDILKED
FGLNARIFFSGNRGFHLRVTCYEDCALLDPDDRKEIAEYFTSPKPPVIYEGNPGWSGRIAKGIEGINIDTQVTIDIRRLV
RIPGSLNGKAGLMVVEIKNDKFEYGEWLSPFDGDCIFLPYVSAELTLFNNKYTVKRTYPIKIDTRIGVYLALKGLGKVKA
YVR

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG1467

COG function: function code L; Eukaryotic-type DNA primase, catalytic (small) subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase small subunit family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIS_SULTO (Q973F6)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376848.1
- ProteinModelPortal:   Q973F6
- SMR:   Q973F6
- GeneID:   1458908
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0943
- NMPDR:   fig|273063.1.peg.1016
- HOGENOM:   HBG512871
- OMA:   FDIDGDH
- ProtClustDB:   PRK00419
- BioCyc:   STOK273063:ST0943-MONOMER
- HAMAP:   MF_00700
- InterPro:   IPR002755
- InterPro:   IPR014052
- PANTHER:   PTHR10536

Pfam domain/function: PF01896 DNA_primase_S

EC number: 2.7.7.-

Molecular weight: Translated: 37245; Mature: 37245

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: ACT_SITE 100-100 ACT_SITE 102-102

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQISTLHQEQNKIIKILFSEYYEKAELDLPNDMELREFAYQPFDSETYVRHLSFSSPQEL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHEECCCCCHHHH
RQYILQNVPLHLYYSSARYQLPSAKEMDEKGWLGSDLLFDLDADEICEVKVRRFCPQDGY
HHHHHHCCCEEEEECCCEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCH
ETLASNCNGEPPIEYAEITTECIMKVFEKALLIRDILKEDFGLNARIFFSGNRGFHLRVT
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEE
CYEDCALLDPDDRKEIAEYFTSPKPPVIYEGNPGWSGRIAKGIEGINIDTQVTIDIRRLV
EECCCEECCCCCHHHHHHHHCCCCCCEEECCCCCCCCHHHCCCCCCCCCEEEEEEEHHHH
RIPGSLNGKAGLMVVEIKNDKFEYGEWLSPFDGDCIFLPYVSAELTLFNNKYTVKRTYPI
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCEEEEEECCEEEEEEEEEE
KIDTRIGVYLALKGLGKVKAYVR
EEECCCEEEEEECCCCCHHHHCC
>Mature Secondary Structure
MQISTLHQEQNKIIKILFSEYYEKAELDLPNDMELREFAYQPFDSETYVRHLSFSSPQEL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHEECCCCCHHHH
RQYILQNVPLHLYYSSARYQLPSAKEMDEKGWLGSDLLFDLDADEICEVKVRRFCPQDGY
HHHHHHCCCEEEEECCCEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCH
ETLASNCNGEPPIEYAEITTECIMKVFEKALLIRDILKEDFGLNARIFFSGNRGFHLRVT
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEE
CYEDCALLDPDDRKEIAEYFTSPKPPVIYEGNPGWSGRIAKGIEGINIDTQVTIDIRRLV
EECCCEECCCCCHHHHHHHHCCCCCCEEECCCCCCCCHHHCCCCCCCCCEEEEEEEHHHH
RIPGSLNGKAGLMVVEIKNDKFEYGEWLSPFDGDCIFLPYVSAELTLFNNKYTVKRTYPI
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCEEEEEECCEEEEEEEEEE
KIDTRIGVYLALKGLGKVKAYVR
EEECCCEEEEEECCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479