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The map label for this gene is ilvI [H]

Identifier: 15920983

GI number: 15920983

Start: 731770

End: 733362

Strand: Reverse

Name: ilvI [H]

Synonym: ST0752

Alternate gene names: 15920983

Gene position: 733362-731770 (Counterclockwise)

Preceding gene: 15920984

Following gene: 15920980

Centisome position: 27.21

GC content: 36.35

Gene sequence:

>1593_bases
ATGGGCAAAACAACATCTGAACTTCTAATTGACACAATTTCTTCCCAAGTAACAGACGTATTCGGAATTCCAGGTACTCA
TGGTTTATCATTATATGAAGAACTCAGAAAGAGAGTAAGTAGAGGAGAGATAACATATTACATGCCTAGACTAGAATATG
GAGGAGCCATAATGGCAGATTATTACGCTAGATTAAAGGGAAATGTTGGAGTTTTCTTGTCAGTTAATGGCCCAGGCTTT
ACTAATTCTTTGACTGGTCTAGTTGAGGCCTATTCTGAAGGTTCTCCTCTTATACTAATATCTCTAAATAAGGAATTTAA
ATATAGGCATAGGAAGCAGCTTCACGATTCTGGGTATTATGATTTACAATTAGAAATGGCTAGACAAGCAACTAAGGCTG
CTTTTAGAATTTACTCCCCAGAAGACATTCCAATTGTAATGCAAAGGGCTTTTAGAATAGCTCTAGAGGACAAGATGGGA
CCAGTTTACATAGAAGTTCCCGTCGATCTACTGGAGGAGAAAAGTAATATTGAAGAGTATAAGACTAACAAGAAAATTAA
TAGGACATTAGTTTACCCTACAAAAGAGGAAATAAAGGAAGCAGTAAATTTCCTAAGTGAGTGTTCAAAACCAATTCTAT
TGTTAGGTTATGGTGCATCTAGGTCGAACATCGTAAACTACATTGAGAGATTAGGAATTCCAGTATTGACTACTATCAGA
GGAAAAGGGAGTATACCAGAGAATCATCCTTTATATGCCGGAACAATATTTAACCTCACTGAAATCCCAGGCGATTGTCT
CATAGCAATAGGGACGTCATTTAACGATCTTGAAACTAATAAATGGAGTATTAAAATGCCGAGGAGGATACTCCACGTAG
ACCCGGACGTCAATGTATTTAATACTTCCTTTAGTGCGGAAGTTACTGTAAAAGCTAGTGCTGAAGCGTTTCTAGAAGAA
ATCGTTGAAAGAGTTAATTTACCAAAGTGGAGTTATAAAGTAGATACCAAAAAGCACATAGAAAGTAATGGTGAAATAAC
TCACGATTATTTAGCCAAAGTTCTAGATGAGACATTAAGTGAAGATAGAGTTATAATTGCTGACGCAGGGACTAATCAGG
TTATGGCAATGGATATAAAAGTGTATAGGCCTAATTCTTACTTTAATTCCCTTATCTTCAACGCAATGGGATCAGCAATT
CCTGCTGGAATAGGAGCTAAAATTGCATCTCCAGAAAGGCAAATAGTAAGTATTATAGGAGATCTAGGATTTCAAGGATG
TTTAAACGAGTTGATTACTGCAGTGCAATATAAGGTCAACTTCCTAACGGTATTAGTAGAGGATGGTGTACAGCACTTTC
TGAGGTTAAATCAGAAAATGAGATATGGGAATACTTTTGCAACTGATGTATTTCAAATTGATTACACTAAGGTTATGGAG
GGAATAGGGGTCAACGTAATTGAGGTAAAGGATAAGGAAGATCTTAAGAAAAATGTAGAAGAAGCTGTTGGATTATCTAT
TAAGAGTCCAACAGTTCTGAGAGTCCACGTTAGCCCTAATAGTATACCTTCTAGATTATTAATGAGAAGATAA

Upstream 100 bases:

>100_bases
AAAATCTCACTCTAATAAGATAAATAGAAATATCGAAAGAAACGCAAATCATGGATTCTTAAAACATCAGATTAATTTAT
GGGAATAATAATAGTAATTT

Downstream 100 bases:

>100_bases
CTATTAATGTAAAATATTATACTATAGTTAAGACACTATTATTTTCTCTTAATCTTGATGAATTTATGAGCACATAGATC
TTGTAAAGGCTTATGATTCT

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MGKTTSELLIDTISSQVTDVFGIPGTHGLSLYEELRKRVSRGEITYYMPRLEYGGAIMADYYARLKGNVGVFLSVNGPGF
TNSLTGLVEAYSEGSPLILISLNKEFKYRHRKQLHDSGYYDLQLEMARQATKAAFRIYSPEDIPIVMQRAFRIALEDKMG
PVYIEVPVDLLEEKSNIEEYKTNKKINRTLVYPTKEEIKEAVNFLSECSKPILLLGYGASRSNIVNYIERLGIPVLTTIR
GKGSIPENHPLYAGTIFNLTEIPGDCLIAIGTSFNDLETNKWSIKMPRRILHVDPDVNVFNTSFSAEVTVKASAEAFLEE
IVERVNLPKWSYKVDTKKHIESNGEITHDYLAKVLDETLSEDRVIIADAGTNQVMAMDIKVYRPNSYFNSLIFNAMGSAI
PAGIGAKIASPERQIVSIIGDLGFQGCLNELITAVQYKVNFLTVLVEDGVQHFLRLNQKMRYGNTFATDVFQIDYTKVME
GIGVNVIEVKDKEDLKKNVEEAVGLSIKSPTVLRVHVSPNSIPSRLLMRR

Sequences:

>Translated_530_residues
MGKTTSELLIDTISSQVTDVFGIPGTHGLSLYEELRKRVSRGEITYYMPRLEYGGAIMADYYARLKGNVGVFLSVNGPGF
TNSLTGLVEAYSEGSPLILISLNKEFKYRHRKQLHDSGYYDLQLEMARQATKAAFRIYSPEDIPIVMQRAFRIALEDKMG
PVYIEVPVDLLEEKSNIEEYKTNKKINRTLVYPTKEEIKEAVNFLSECSKPILLLGYGASRSNIVNYIERLGIPVLTTIR
GKGSIPENHPLYAGTIFNLTEIPGDCLIAIGTSFNDLETNKWSIKMPRRILHVDPDVNVFNTSFSAEVTVKASAEAFLEE
IVERVNLPKWSYKVDTKKHIESNGEITHDYLAKVLDETLSEDRVIIADAGTNQVMAMDIKVYRPNSYFNSLIFNAMGSAI
PAGIGAKIASPERQIVSIIGDLGFQGCLNELITAVQYKVNFLTVLVEDGVQHFLRLNQKMRYGNTFATDVFQIDYTKVME
GIGVNVIEVKDKEDLKKNVEEAVGLSIKSPTVLRVHVSPNSIPSRLLMRR
>Mature_529_residues
GKTTSELLIDTISSQVTDVFGIPGTHGLSLYEELRKRVSRGEITYYMPRLEYGGAIMADYYARLKGNVGVFLSVNGPGFT
NSLTGLVEAYSEGSPLILISLNKEFKYRHRKQLHDSGYYDLQLEMARQATKAAFRIYSPEDIPIVMQRAFRIALEDKMGP
VYIEVPVDLLEEKSNIEEYKTNKKINRTLVYPTKEEIKEAVNFLSECSKPILLLGYGASRSNIVNYIERLGIPVLTTIRG
KGSIPENHPLYAGTIFNLTEIPGDCLIAIGTSFNDLETNKWSIKMPRRILHVDPDVNVFNTSFSAEVTVKASAEAFLEEI
VERVNLPKWSYKVDTKKHIESNGEITHDYLAKVLDETLSEDRVIIADAGTNQVMAMDIKVYRPNSYFNSLIFNAMGSAIP
AGIGAKIASPERQIVSIIGDLGFQGCLNELITAVQYKVNFLTVLVEDGVQHFLRLNQKMRYGNTFATDVFQIDYTKVMEG
IGVNVIEVKDKEDLKKNVEEAVGLSIKSPTVLRVHVSPNSIPSRLLMRR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=527, Percent_Identity=23.5294117647059, Blast_Score=119, Evalue=8e-27,
Organism=Escherichia coli, GI87081685, Length=540, Percent_Identity=27.4074074074074, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1786717, Length=549, Percent_Identity=25.1366120218579, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1790104, Length=539, Percent_Identity=24.30426716141, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788716, Length=482, Percent_Identity=24.4813278008299, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1787096, Length=566, Percent_Identity=22.791519434629, Blast_Score=113, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17531301, Length=505, Percent_Identity=23.3663366336634, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17531299, Length=505, Percent_Identity=23.3663366336634, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17542570, Length=520, Percent_Identity=22.3076923076923, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=566, Percent_Identity=24.7349823321555, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6323163, Length=462, Percent_Identity=22.7272727272727, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321524, Length=470, Percent_Identity=23.8297872340426, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=467, Percent_Identity=22.2698072805139, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320816, Length=541, Percent_Identity=22.735674676525, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922626, Length=579, Percent_Identity=23.8341968911917, Blast_Score=119, Evalue=5e-27,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59431; Mature: 59300

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTTSELLIDTISSQVTDVFGIPGTHGLSLYEELRKRVSRGEITYYMPRLEYGGAIMAD
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHH
YYARLKGNVGVFLSVNGPGFTNSLTGLVEAYSEGSPLILISLNKEFKYRHRKQLHDSGYY
HHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCE
DLQLEMARQATKAAFRIYSPEDIPIVMQRAFRIALEDKMGPVYIEVPVDLLEEKSNIEEY
EEEHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHEECCCCCEEEEECHHHHHCCCCHHHH
KTNKKINRTLVYPTKEEIKEAVNFLSECSKPILLLGYGASRSNIVNYIERLGIPVLTTIR
HCCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEC
GKGSIPENHPLYAGTIFNLTEIPGDCLIAIGTSFNDLETNKWSIKMPRRILHVDPDVNVF
CCCCCCCCCCEEEEEEEEHHHCCCCEEEEECCCCCCCCCCEEEEECCCEEEEECCCCEEE
NTSFSAEVTVKASAEAFLEEIVERVNLPKWSYKVDTKKHIESNGEITHDYLAKVLDETLS
ECCCEEEEEEECCHHHHHHHHHHHHCCCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHC
EDRVIIADAGTNQVMAMDIKVYRPNSYFNSLIFNAMGSAIPAGIGAKIASPERQIVSIIG
CCEEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
DLGFQGCLNELITAVQYKVNFLTVLVEDGVQHFLRLNQKMRYGNTFATDVFQIDYTKVME
HCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHCCCCEEHHEEEEEHHHHHH
GIGVNVIEVKDKEDLKKNVEEAVGLSIKSPTVLRVHVSPNSIPSRLLMRR
CCCCEEEEECCHHHHHHHHHHHHCCEECCCEEEEEEECCCCCHHHHHHCC
>Mature Secondary Structure 
GKTTSELLIDTISSQVTDVFGIPGTHGLSLYEELRKRVSRGEITYYMPRLEYGGAIMAD
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHH
YYARLKGNVGVFLSVNGPGFTNSLTGLVEAYSEGSPLILISLNKEFKYRHRKQLHDSGYY
HHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCE
DLQLEMARQATKAAFRIYSPEDIPIVMQRAFRIALEDKMGPVYIEVPVDLLEEKSNIEEY
EEEHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHEECCCCCEEEEECHHHHHCCCCHHHH
KTNKKINRTLVYPTKEEIKEAVNFLSECSKPILLLGYGASRSNIVNYIERLGIPVLTTIR
HCCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEC
GKGSIPENHPLYAGTIFNLTEIPGDCLIAIGTSFNDLETNKWSIKMPRRILHVDPDVNVF
CCCCCCCCCCEEEEEEEEHHHCCCCEEEEECCCCCCCCCCEEEEECCCEEEEECCCCEEE
NTSFSAEVTVKASAEAFLEEIVERVNLPKWSYKVDTKKHIESNGEITHDYLAKVLDETLS
ECCCEEEEEEECCHHHHHHHHHHHHCCCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHC
EDRVIIADAGTNQVMAMDIKVYRPNSYFNSLIFNAMGSAIPAGIGAKIASPERQIVSIIG
CCEEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
DLGFQGCLNELITAVQYKVNFLTVLVEDGVQHFLRLNQKMRYGNTFATDVFQIDYTKVME
HCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHCCCCEEHHEEEEEHHHHHH
GIGVNVIEVKDKEDLKKNVEEAVGLSIKSPTVLRVHVSPNSIPSRLLMRR
CCCCEEEEECCHHHHHHHHHHHHCCEECCCEEEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10555290 [H]