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The map label for this gene is leuD

Identifier: 15920820

GI number: 15920820

Start: 587578

End: 588084

Strand: Direct

Name: leuD

Synonym: ST0600

Alternate gene names: 15920820

Gene position: 587578-588084 (Clockwise)

Preceding gene: 15920819

Following gene: 15920823

Centisome position: 21.8

GC content: 36.29

Gene sequence:

>507_bases
ATGATAGTAGAAGGTAAGGTTTTAAAATTCGGTGATAAAATAGATACTGACATCATTATACCAGCAAGGTATCTGAAATA
CACTGATCCGCAATACTTAGCACAGCACGCAATGGAACCTCTAGATCCAGAATTTTATAAAAAAGCTTCTGCTGGTGTAA
TTTTAGTTGCTGGCAAAGTCTTTGGAATGGGTTCATCAAGAGAGCAAGCTGCTATTGCATTAAAGGCTGCTGGTGTTAGG
GCTATTATAGCTGAATCGTTTGCAAGAATATTCTATAGGAATGCTATAAATAATGGGTTGCCAGCTATTGTTTTACCTGG
TGTAACTCAGCTAATAAACGAAGGTGATTATGTAAAGATAAATGTAGAAACCGGAGAAATAATAGTGAATAATCAGAAAG
TTTATAAGGGAAGAGGAATAACTGGAATGCCGTTAAAAATACTTGAGAGCGGCGGTTTACTGGATTATTTAAAGAAAGTT
TCGGTTCAGAATCAAGGAGGTAACTGA

Upstream 100 bases:

>100_bases
GTAGAATGGGGAGTAACGATTCAAAAGTATATTTAGCTGGACCAGCAGTTGCAGCAGCTTCTGCTTTACAAGGCAAAATA
ACTGATCCGAGGAGATTCTC

Downstream 100 bases:

>100_bases
AATAAAAAAGTTTGTATACAACTTTTTTAAATTATTTCTTATGAGGTTTACTTTCGTTATACTCTCCCTCATTTTTATGT
TTATAGACGAGTCTTTTTCA

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MIVEGKVLKFGDKIDTDIIIPARYLKYTDPQYLAQHAMEPLDPEFYKKASAGVILVAGKVFGMGSSREQAAIALKAAGVR
AIIAESFARIFYRNAINNGLPAIVLPGVTQLINEGDYVKINVETGEIIVNNQKVYKGRGITGMPLKILESGGLLDYLKKV
SVQNQGGN

Sequences:

>Translated_168_residues
MIVEGKVLKFGDKIDTDIIIPARYLKYTDPQYLAQHAMEPLDPEFYKKASAGVILVAGKVFGMGSSREQAAIALKAAGVR
AIIAESFARIFYRNAINNGLPAIVLPGVTQLINEGDYVKINVETGEIIVNNQKVYKGRGITGMPLKILESGGLLDYLKKV
SVQNQGGN
>Mature_168_residues
MIVEGKVLKFGDKIDTDIIIPARYLKYTDPQYLAQHAMEPLDPEFYKKASAGVILVAGKVFGMGSSREQAAIALKAAGVR
AIIAESFARIFYRNAINNGLPAIVLPGVTQLINEGDYVKINVETGEIIVNNQKVYKGRGITGMPLKILESGGLLDYLKKV
SVQNQGGN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=144, Percent_Identity=32.6388888888889, Blast_Score=68, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320440, Length=110, Percent_Identity=43.6363636363636, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_SULTO (Q974Q9)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376489.1
- ProteinModelPortal:   Q974Q9
- SMR:   Q974Q9
- GeneID:   1458548
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0600
- NMPDR:   fig|273063.1.peg.657
- HOGENOM:   HBG304838
- OMA:   VLGQHAM
- ProtClustDB:   PRK00439
- BioCyc:   STOK273063:ST0600-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 18226; Mature: 18226

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVEGKVLKFGDKIDTDIIIPARYLKYTDPQYLAQHAMEPLDPEFYKKASAGVILVAGKV
CCCCCCEEECCCCCCCEEEECHHHHCCCCHHHHHHHCCCCCCHHHHHHCCCCEEEEECEE
FGMGSSREQAAIALKAAGVRAIIAESFARIFYRNAINNGLPAIVLPGVTQLINEGDYVKI
ECCCCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCEEEE
NVETGEIIVNNQKVYKGRGITGMPLKILESGGLLDYLKKVSVQNQGGN
EEECCEEEECCCEEEECCCCCCCCHHEECCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIVEGKVLKFGDKIDTDIIIPARYLKYTDPQYLAQHAMEPLDPEFYKKASAGVILVAGKV
CCCCCCEEECCCCCCCEEEECHHHHCCCCHHHHHHHCCCCCCHHHHHHCCCCEEEEECEE
FGMGSSREQAAIALKAAGVRAIIAESFARIFYRNAINNGLPAIVLPGVTQLINEGDYVKI
ECCCCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCEEEE
NVETGEIIVNNQKVYKGRGITGMPLKILESGGLLDYLKKVSVQNQGGN
EEECCEEEECCCEEEECCCCCCCCHHEECCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479