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The map label for this gene is leuC

Identifier: 15920819

GI number: 15920819

Start: 586334

End: 587581

Strand: Direct

Name: leuC

Synonym: ST0599

Alternate gene names: 15920819

Gene position: 586334-587581 (Clockwise)

Preceding gene: 15920818

Following gene: 15920820

Centisome position: 21.76

GC content: 36.46

Gene sequence:

>1248_bases
GTGCCTCAAACTTTAACGGAAAAAATCCTAAGTAGAGCCTCGGGTAAATCAGTAGCTCCAGGAGATGTAGTGGAGGTTAA
AGTAGATATTGCAGCTTTTCATGACTTGACTGGGTATCATGTAATAGAAGTTATGGAGAAAGCGGGTATGTTAAAAGTTT
TTGATAAACAAAAAATTGTAATAGCTTTTGATCATTTAGCTCCTCCACCAGATGTAAGAAGTGCAGAGATTCAAACTCAA
ATAAGAAAATTTGTAAAAGAACTAAAGATACCAAACTTCCATGATATAAATGTAGGTATCTTACATCAAATATTACTAGA
AAAATACGCTAATCCAGGATATGTAATAGTAGCTGCTGATAGTCATACTACAACTTCTGGTGCTGTTGGAGCTTTCGCAC
AAGGTTTAGGTGCAAGTGATGTTGCAGCTGCAGTTATAACTGGCAAAACATGGGTTATGGTTCCACAATCATTTAAGATA
ATGTTAGAAGGTAAACCTGGTAAATGGATAAATGGAAAAGACGTAGCATTAAAGATCCTTGGCGATTTTAAGGCTGACTA
CTTTAATGGAATGAGTATTGAAATATTTGTAAGGGAACCGTCTGCATTTCCAATGGACTTTAGGGCAACAGTTTCTAATA
TGGGAATAGAAATGAATGCTGACGCGTTAATGTTTGTTCCAGATGAAGAGACAGTTAATTACATTAAGACTAACAGAGGT
TATGAACCAAATATTGTTAGACCAGATGAAGGAGCAAAGTATGTTGATGAATATACGATTGATTTAGGAAAATTAGAACC
CTTAGTTGCTGCTCCTCATAGTGTTGATAACGTTAAAACAGTTAATGAAGTTGAAGGTTTAGATGTAGATCAAGTATTCA
TAGGATCTTGTACAAATGGTAGAATAAGTGATTTCGAGATTGCTGCAAAAATTCTAAAGGGAAAGAGAGTTAAGAGTAGA
TGCATAGCAATACCAGCATCTTATGATTTATTTAAGAAGGCTATGGAGCTAGGCTATATAGAGACTTTAGTTAATGCTGG
ATGTATTGTTACATATGGTACTTGTGGACCATGTCTAGGAGGTCACTTTGGTGTGGCTGGCCCAGGTGAGACAATAGTTT
CGACTAGTTCAAGGAACTTTAAAGGTAGAATGGGGAGTAACGATTCAAAAGTATATTTAGCTGGACCAGCAGTTGCAGCA
GCTTCTGCTTTACAAGGCAAAATAACTGATCCGAGGAGATTCTCATGA

Upstream 100 bases:

>100_bases
AAAGAGAAATACTAAAAACAAAAACCAATTACTAGTCAAATAACAAAAGTCGAACATATTTTAAAATAATATTTATTTTC
TCTGACTCTTTCTTATACTC

Downstream 100 bases:

>100_bases
TAGTAGAAGGTAAGGTTTTAAAATTCGGTGATAAAATAGATACTGACATCATTATACCAGCAAGGTATCTGAAATACACT
GATCCGCAATACTTAGCACA

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MPQTLTEKILSRASGKSVAPGDVVEVKVDIAAFHDLTGYHVIEVMEKAGMLKVFDKQKIVIAFDHLAPPPDVRSAEIQTQ
IRKFVKELKIPNFHDINVGILHQILLEKYANPGYVIVAADSHTTTSGAVGAFAQGLGASDVAAAVITGKTWVMVPQSFKI
MLEGKPGKWINGKDVALKILGDFKADYFNGMSIEIFVREPSAFPMDFRATVSNMGIEMNADALMFVPDEETVNYIKTNRG
YEPNIVRPDEGAKYVDEYTIDLGKLEPLVAAPHSVDNVKTVNEVEGLDVDQVFIGSCTNGRISDFEIAAKILKGKRVKSR
CIAIPASYDLFKKAMELGYIETLVNAGCIVTYGTCGPCLGGHFGVAGPGETIVSTSSRNFKGRMGSNDSKVYLAGPAVAA
ASALQGKITDPRRFS

Sequences:

>Translated_415_residues
MPQTLTEKILSRASGKSVAPGDVVEVKVDIAAFHDLTGYHVIEVMEKAGMLKVFDKQKIVIAFDHLAPPPDVRSAEIQTQ
IRKFVKELKIPNFHDINVGILHQILLEKYANPGYVIVAADSHTTTSGAVGAFAQGLGASDVAAAVITGKTWVMVPQSFKI
MLEGKPGKWINGKDVALKILGDFKADYFNGMSIEIFVREPSAFPMDFRATVSNMGIEMNADALMFVPDEETVNYIKTNRG
YEPNIVRPDEGAKYVDEYTIDLGKLEPLVAAPHSVDNVKTVNEVEGLDVDQVFIGSCTNGRISDFEIAAKILKGKRVKSR
CIAIPASYDLFKKAMELGYIETLVNAGCIVTYGTCGPCLGGHFGVAGPGETIVSTSSRNFKGRMGSNDSKVYLAGPAVAA
ASALQGKITDPRRFS
>Mature_414_residues
PQTLTEKILSRASGKSVAPGDVVEVKVDIAAFHDLTGYHVIEVMEKAGMLKVFDKQKIVIAFDHLAPPPDVRSAEIQTQI
RKFVKELKIPNFHDINVGILHQILLEKYANPGYVIVAADSHTTTSGAVGAFAQGLGASDVAAAVITGKTWVMVPQSFKIM
LEGKPGKWINGKDVALKILGDFKADYFNGMSIEIFVREPSAFPMDFRATVSNMGIEMNADALMFVPDEETVNYIKTNRGY
EPNIVRPDEGAKYVDEYTIDLGKLEPLVAAPHSVDNVKTVNEVEGLDVDQVFIGSCTNGRISDFEIAAKILKGKRVKSRC
IAIPASYDLFKKAMELGYIETLVNAGCIVTYGTCGPCLGGHFGVAGPGETIVSTSSRNFKGRMGSNDSKVYLAGPAVAAA
SALQGKITDPRRFS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=447, Percent_Identity=29.3064876957494, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI8659555, Length=405, Percent_Identity=22.962962962963, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1786259, Length=388, Percent_Identity=32.4742268041237, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI87081781, Length=347, Percent_Identity=26.8011527377522, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI2367097, Length=461, Percent_Identity=26.2472885032538, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25149337, Length=448, Percent_Identity=26.7857142857143, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI32564738, Length=337, Percent_Identity=29.3768545994065, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=285, Percent_Identity=27.0175438596491, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320440, Length=445, Percent_Identity=33.0337078651685, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6323335, Length=448, Percent_Identity=29.0178571428571, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6321429, Length=475, Percent_Identity=29.0526315789474, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=339, Percent_Identity=28.9085545722714, Blast_Score=122, Evalue=8e-29,
Organism=Drosophila melanogaster, GI28571643, Length=448, Percent_Identity=28.3482142857143, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI281365315, Length=448, Percent_Identity=26.3392857142857, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17864292, Length=448, Percent_Identity=26.3392857142857, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161076999, Length=337, Percent_Identity=28.486646884273, Blast_Score=108, Evalue=5e-24,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_SULTO (Q974R0)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376488.1
- ProteinModelPortal:   Q974R0
- GeneID:   1458547
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0599
- NMPDR:   fig|273063.1.peg.656
- HOGENOM:   HBG330745
- OMA:   GADALMF
- ProtClustDB:   PRK00402
- BioCyc:   STOK273063:ST0599-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 44853; Mature: 44722

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQTLTEKILSRASGKSVAPGDVVEVKVDIAAFHDLTGYHVIEVMEKAGMLKVFDKQKIV
CCHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHCCCEEEECCCEEE
IAFDHLAPPPDVRSAEIQTQIRKFVKELKIPNFHDINVGILHQILLEKYANPGYVIVAAD
EEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEC
SHTTTSGAVGAFAQGLGASDVAAAVITGKTWVMVPQSFKIMLEGKPGKWINGKDVALKIL
CCCCCCCCHHHHHHCCCCCHHEEEEEECCEEEEECCCEEEEEECCCCCCCCCCCEEEEEE
GDFKADYFNGMSIEIFVREPSAFPMDFRATVSNMGIEMNADALMFVPDEETVNYIKTNRG
ECCCHHHHCCCEEEEEEECCCCCCCHHHHHHHHCCEEECCCEEEEECCHHHHHHHCCCCC
YEPNIVRPDEGAKYVDEYTIDLGKLEPLVAAPHSVDNVKTVNEVEGLDVDQVFIGSCTNG
CCCCEECCCCCCHHHHHHEECCCCCCCHHCCCCCCCCCCHHHHHCCCCCCEEEEECCCCC
RISDFEIAAKILKGKRVKSRCIAIPASYDLFKKAMELGYIETLVNAGCIVTYGTCGPCLG
CCCHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
GHFGVAGPGETIVSTSSRNFKGRMGSNDSKVYLAGPAVAAASALQGKITDPRRFS
CCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PQTLTEKILSRASGKSVAPGDVVEVKVDIAAFHDLTGYHVIEVMEKAGMLKVFDKQKIV
CHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHCCCEEEECCCEEE
IAFDHLAPPPDVRSAEIQTQIRKFVKELKIPNFHDINVGILHQILLEKYANPGYVIVAAD
EEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEC
SHTTTSGAVGAFAQGLGASDVAAAVITGKTWVMVPQSFKIMLEGKPGKWINGKDVALKIL
CCCCCCCCHHHHHHCCCCCHHEEEEEECCEEEEECCCEEEEEECCCCCCCCCCCEEEEEE
GDFKADYFNGMSIEIFVREPSAFPMDFRATVSNMGIEMNADALMFVPDEETVNYIKTNRG
ECCCHHHHCCCEEEEEEECCCCCCCHHHHHHHHCCEEECCCEEEEECCHHHHHHHCCCCC
YEPNIVRPDEGAKYVDEYTIDLGKLEPLVAAPHSVDNVKTVNEVEGLDVDQVFIGSCTNG
CCCCEECCCCCCHHHHHHEECCCCCCCHHCCCCCCCCCCHHHHHCCCCCCEEEEECCCCC
RISDFEIAAKILKGKRVKSRCIAIPASYDLFKKAMELGYIETLVNAGCIVTYGTCGPCLG
CCCHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
GHFGVAGPGETIVSTSSRNFKGRMGSNDSKVYLAGPAVAAASALQGKITDPRRFS
CCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479