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The map label for this gene is rtcA

Identifier: 15920788

GI number: 15920788

Start: 560956

End: 561975

Strand: Reverse

Name: rtcA

Synonym: ST0570

Alternate gene names: 15920788

Gene position: 561975-560956 (Counterclockwise)

Preceding gene: 15920791

Following gene: 15920783

Centisome position: 20.85

GC content: 33.33

Gene sequence:

>1020_bases
ATGATTGAAATAGACGGTTCATTTGGTGAAGGAGGAGGACAAATTTTAAGAACATCTTTAACACTTTCAGCATTAACTAA
GAAACCATTCAGAATATATAAAATTAGAGCAAATAGACCAAAACCTGGTTTACAAAGACAGCATCTTACAGCTGTTGAGG
CAGTAAAAAAATTAACTAATGCTAAAGTAAAAGGAGACTTTGTAGGATCAACTGAACTAGTTTTCGAACCTGAAGATATA
GTAGAAAAAGGAGACTTTGAATTTGACGTAGGAACTGCGGGAAGTGTAACCTTAATTCTACAAACAATACTGCCTTTATT
AATTAATAGGAATATAAAAGTTACCATTAAAGGAGGTACGGATGTTCCTAAATCTCCTTCAATAGATTATATACGATTAA
CCTTTCTCTCTCTGTTAGAAAAAATAGGAATTAGAGTAAATTTAATACTTATTAGAAGAGGACATTACCCAGAAGGAGGA
GGAGAAATCAAAATTACAGAAGTAAAAGGAAACCCTTCTTCATTCTCTTTAATGGAAAGAGGAGAGTTATTAATGATAAA
AGGGATCTCCCACGTATCGTCTTTACCATCACACATAGCTGAGAGACAAGCTAAGTCAGCTAAAGAATTTCTTCTCTCAA
AAATAAAGATTCCAGTAGAAATAGAAATTGATGTCAGGGAAAATGAAAGAAGTAAGGGCTCCGGTATCGCATTAACAGCG
ATTTTTGAAAAAACTTTTCTTGGCTCCGACTCTTTAGGAGAAAAGGGTAAAAGGGCTGAAATAGTAGGTGAAGAAGCAGC
TAAATCTATCTATGAAGAAATTATAAGCAATGCTACCGTCGATAGACATATGAGCGATATGTTAATGCTATATGCTTCAT
TATATTATGGCGAATATATAGGTTCTGAACTCACATCTCATGCAAGAACTAATTCAGAGATTATCAAAAAGTTTCTTAAT
GTGAATATTCAAATTTCTGGCGAAAAACCATTCATTTTTAGAGCTAAGAAGGAATTATGA

Upstream 100 bases:

>100_bases
AAATCACCGTTTCGGGTAATTGGAATAATTATTAACTCTTCTGTGCCTATAATTTTGGCAATAGCATTTATTTTCACACG
CATTCCTATATTCACCAGTC

Downstream 100 bases:

>100_bases
CAGCCCTTCCCAAAATTCTCTCTTGCATTAAATCATTTAAGGCATCATTTATTTCATCTAGATAATAAGGTACGGCAAGA
GTTTTTAACTTACCAGAATT

Product: RNA 3'-terminal-phosphate cyclase

Products: NA

Alternate protein names: RNA cyclase; RNA-3'-phosphate cyclase

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MIEIDGSFGEGGGQILRTSLTLSALTKKPFRIYKIRANRPKPGLQRQHLTAVEAVKKLTNAKVKGDFVGSTELVFEPEDI
VEKGDFEFDVGTAGSVTLILQTILPLLINRNIKVTIKGGTDVPKSPSIDYIRLTFLSLLEKIGIRVNLILIRRGHYPEGG
GEIKITEVKGNPSSFSLMERGELLMIKGISHVSSLPSHIAERQAKSAKEFLLSKIKIPVEIEIDVRENERSKGSGIALTA
IFEKTFLGSDSLGEKGKRAEIVGEEAAKSIYEEIISNATVDRHMSDMLMLYASLYYGEYIGSELTSHARTNSEIIKKFLN
VNIQISGEKPFIFRAKKEL

Sequences:

>Translated_339_residues
MIEIDGSFGEGGGQILRTSLTLSALTKKPFRIYKIRANRPKPGLQRQHLTAVEAVKKLTNAKVKGDFVGSTELVFEPEDI
VEKGDFEFDVGTAGSVTLILQTILPLLINRNIKVTIKGGTDVPKSPSIDYIRLTFLSLLEKIGIRVNLILIRRGHYPEGG
GEIKITEVKGNPSSFSLMERGELLMIKGISHVSSLPSHIAERQAKSAKEFLLSKIKIPVEIEIDVRENERSKGSGIALTA
IFEKTFLGSDSLGEKGKRAEIVGEEAAKSIYEEIISNATVDRHMSDMLMLYASLYYGEYIGSELTSHARTNSEIIKKFLN
VNIQISGEKPFIFRAKKEL
>Mature_339_residues
MIEIDGSFGEGGGQILRTSLTLSALTKKPFRIYKIRANRPKPGLQRQHLTAVEAVKKLTNAKVKGDFVGSTELVFEPEDI
VEKGDFEFDVGTAGSVTLILQTILPLLINRNIKVTIKGGTDVPKSPSIDYIRLTFLSLLEKIGIRVNLILIRRGHYPEGG
GEIKITEVKGNPSSFSLMERGELLMIKGISHVSSLPSHIAERQAKSAKEFLLSKIKIPVEIEIDVRENERSKGSGIALTA
IFEKTFLGSDSLGEKGKRAEIVGEEAAKSIYEEIISNATVDRHMSDMLMLYASLYYGEYIGSELTSHARTNSEIIKKFLN
VNIQISGEKPFIFRAKKEL

Specific function: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A;

COG id: COG0430

COG function: function code A; RNA 3'-terminal phosphate cyclase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4506589, Length=307, Percent_Identity=37.4592833876222, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI195927044, Length=320, Percent_Identity=35.9375, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI157426877, Length=304, Percent_Identity=27.3026315789474, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI48994937, Length=337, Percent_Identity=38.8724035608309, Blast_Score=208, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI32564710, Length=288, Percent_Identity=26.0416666666667, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324564, Length=315, Percent_Identity=25.7142857142857, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI18543269, Length=338, Percent_Identity=31.6568047337278, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI18860065, Length=343, Percent_Identity=23.6151603498542, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RTCA_SULTO (Q974U1)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376457.1
- ProteinModelPortal:   Q974U1
- SMR:   Q974U1
- GeneID:   1458516
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0570
- NMPDR:   fig|273063.1.peg.625
- HOGENOM:   HBG683539
- OMA:   EIDGSYG
- ProtClustDB:   PRK04204
- BioCyc:   STOK273063:ST0570-MONOMER
- BRENDA:   6.5.1.4
- GO:   GO:0005737
- GO:   GO:0005654
- HAMAP:   MF_00200
- InterPro:   IPR000228
- InterPro:   IPR020719
- InterPro:   IPR013796
- InterPro:   IPR020723
- InterPro:   IPR017770
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.20
- PANTHER:   PTHR11096
- TIGRFAMs:   TIGR03399

Pfam domain/function: PF01137 RTC; PF05189 RTC_insert; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =6.5.1.4

Molecular weight: Translated: 37506; Mature: 37506

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS01287 RTC

Important sites: ACT_SITE 307-307 BINDING 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIDGSFGEGGGQILRTSLTLSALTKKPFRIYKIRANRPKPGLQRQHLTAVEAVKKLTN
CEEECCCCCCCCCCEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHC
AKVKGDFVGSTELVFEPEDIVEKGDFEFDVGTAGSVTLILQTILPLLINRNIKVTIKGGT
CCCCCCCCCCEEEEECCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCC
DVPKSPSIDYIRLTFLSLLEKIGIRVNLILIRRGHYPEGGGEIKITEVKGNPSSFSLMER
CCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCEEEEEEECCCCCCCHHHCC
GELLMIKGISHVSSLPSHIAERQAKSAKEFLLSKIKIPVEIEIDVRENERSKGSGIALTA
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEE
IFEKTFLGSDSLGEKGKRAEIVGEEAAKSIYEEIISNATVDRHMSDMLMLYASLYYGEYI
EEHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GSELTSHARTNSEIIKKFLNVNIQISGEKPFIFRAKKEL
HHHHHHHHCCCHHHHHHHHCCEEEECCCCCEEEEECCCC
>Mature Secondary Structure
MIEIDGSFGEGGGQILRTSLTLSALTKKPFRIYKIRANRPKPGLQRQHLTAVEAVKKLTN
CEEECCCCCCCCCCEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHC
AKVKGDFVGSTELVFEPEDIVEKGDFEFDVGTAGSVTLILQTILPLLINRNIKVTIKGGT
CCCCCCCCCCEEEEECCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCC
DVPKSPSIDYIRLTFLSLLEKIGIRVNLILIRRGHYPEGGGEIKITEVKGNPSSFSLMER
CCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCEEEEEEECCCCCCCHHHCC
GELLMIKGISHVSSLPSHIAERQAKSAKEFLLSKIKIPVEIEIDVRENERSKGSGIALTA
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEE
IFEKTFLGSDSLGEKGKRAEIVGEEAAKSIYEEIISNATVDRHMSDMLMLYASLYYGEYI
EEHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GSELTSHARTNSEIIKKFLNVNIQISGEKPFIFRAKKEL
HHHHHHHHCCCHHHHHHHHCCEEEECCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479