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The map label for this gene is gatA [H]

Identifier: 15920693

GI number: 15920693

Start: 478453

End: 479643

Strand: Reverse

Name: gatA [H]

Synonym: ST0478

Alternate gene names: 15920693

Gene position: 479643-478453 (Counterclockwise)

Preceding gene: 15920694

Following gene: 15920692

Centisome position: 17.8

GC content: 31.82

Gene sequence:

>1191_bases
ATGAAGATGTCATTAGAAGAATTAAATACAAAATACAATATTTTCATCACAATAAGCAGAATGAAATATAGAGATAAAGG
TAAACTAAGAGACCTAAAATTCGGAATAAAAGATATCATTTTAACTAAAGGTGTAAGAACAACTGCTGGTTCAAAAATAT
TAAAAGATTTCATACCTAGTGAGAACGCATATATTGTTGATAAAATATTAGCTGAAGGAGGAGAGATAATAGGAAAAACT
AATACTCATGAATTTGCTATTGGTGCTACTAATACCTCATCTCTAATTGGACCAGCAAAAAATCCCTATGATCCAGAGAG
AATAAGTGGAGGATCTAGTGGAGGATCAGCAGTGGCAGTAGCATTAGGTTTAGTTGATGTTGGTATAGGAACAGATACTG
GAGGGTCTGTAAGAATTCCAGCATCATTATGTGGAGTCATAGGTTTTAAGCCAACCTACGGAATTATACCTACTGATGGC
GTAATTCCTTTCAGTTGGACATTTGACACTATAGGTTTCTTAGCAAAAGATATCTCATTAATAAGAAGAACTATTGAAGC
TCTTGCTGAAAATAAAATTCCTTTTATATCATACTCTAAGAGAAGGCCTACGTTAGGATTATTCCTATTTGGAGATTATG
AAACTTCTAAGGCCTTAGAACCAGTAATAAATAAACTTTCATCCTATTTTGATTTAGTACAGATAGAGAATGAACTTCTG
ATTAAACATTCTAGAATTGTAAGAAAAACTATTGCTTTAGCAGAAGCTTCATCTTATCATATGAAATATTACGATAAATA
TAAAGACTTGTATTCGTCTGATGTTAGAAAATTAATCGAAGAAGGCTTCAAAATTTTGGCTACAGATTACGTCAACTCTT
TAAGAATTAGAAAAGTATTGTTAGAGGAATACTTCTCTATCTTTAAAAAAATAGATGCATTAATTTCACCTACAACAAGA
ATTGTCGCACCTAAGATTCAAGAAGTAATAGGGAATGAACTAAAATATAGAGACTCTTTAATTGCTAATACCGAAATATT
TAATACTTTAGGAGCTCCATCAATCTCAATTCCAGTAACTAAGCTAAATGATTTACCTGTGGGGTTAATGATTAGCGGAG
AGCCCTATAAAGATGGAACTGTTTTAGATATTGCAGAGGAAATAATGGGAATTGTTGGTTTTATTAAGTAG

Upstream 100 bases:

>100_bases
ACGTTAATAGGTGGGAATTGGAAAATTTTATAAAGGTAATCCTTGATGAACTTAGAAGATTACATCTTAGAAAGACTTAA
TGAGCCTTTATTCTGCGTGA

Downstream 100 bases:

>100_bases
TTTTACACAATTTGTTTTGATGGAGACTAATAATAAGTTAAAGATTTTAAAAACCGGAACTACCACGGTTGGAATAAAAG
TAAAAGATGGAGTAGTATTA

Product: enantiomer-selective amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MKMSLEELNTKYNIFITISRMKYRDKGKLRDLKFGIKDIILTKGVRTTAGSKILKDFIPSENAYIVDKILAEGGEIIGKT
NTHEFAIGATNTSSLIGPAKNPYDPERISGGSSGGSAVAVALGLVDVGIGTDTGGSVRIPASLCGVIGFKPTYGIIPTDG
VIPFSWTFDTIGFLAKDISLIRRTIEALAENKIPFISYSKRRPTLGLFLFGDYETSKALEPVINKLSSYFDLVQIENELL
IKHSRIVRKTIALAEASSYHMKYYDKYKDLYSSDVRKLIEEGFKILATDYVNSLRIRKVLLEEYFSIFKKIDALISPTTR
IVAPKIQEVIGNELKYRDSLIANTEIFNTLGAPSISIPVTKLNDLPVGLMISGEPYKDGTVLDIAEEIMGIVGFIK

Sequences:

>Translated_396_residues
MKMSLEELNTKYNIFITISRMKYRDKGKLRDLKFGIKDIILTKGVRTTAGSKILKDFIPSENAYIVDKILAEGGEIIGKT
NTHEFAIGATNTSSLIGPAKNPYDPERISGGSSGGSAVAVALGLVDVGIGTDTGGSVRIPASLCGVIGFKPTYGIIPTDG
VIPFSWTFDTIGFLAKDISLIRRTIEALAENKIPFISYSKRRPTLGLFLFGDYETSKALEPVINKLSSYFDLVQIENELL
IKHSRIVRKTIALAEASSYHMKYYDKYKDLYSSDVRKLIEEGFKILATDYVNSLRIRKVLLEEYFSIFKKIDALISPTTR
IVAPKIQEVIGNELKYRDSLIANTEIFNTLGAPSISIPVTKLNDLPVGLMISGEPYKDGTVLDIAEEIMGIVGFIK
>Mature_396_residues
MKMSLEELNTKYNIFITISRMKYRDKGKLRDLKFGIKDIILTKGVRTTAGSKILKDFIPSENAYIVDKILAEGGEIIGKT
NTHEFAIGATNTSSLIGPAKNPYDPERISGGSSGGSAVAVALGLVDVGIGTDTGGSVRIPASLCGVIGFKPTYGIIPTDG
VIPFSWTFDTIGFLAKDISLIRRTIEALAENKIPFISYSKRRPTLGLFLFGDYETSKALEPVINKLSSYFDLVQIENELL
IKHSRIVRKTIALAEASSYHMKYYDKYKDLYSSDVRKLIEEGFKILATDYVNSLRIRKVLLEEYFSIFKKIDALISPTTR
IVAPKIQEVIGNELKYRDSLIANTEIFNTLGAPSISIPVTKLNDLPVGLMISGEPYKDGTVLDIAEEIMGIVGFIK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=451, Percent_Identity=29.2682926829268, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI195972892, Length=405, Percent_Identity=21.4814814814815, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=435, Percent_Identity=30.3448275862069, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17537465, Length=142, Percent_Identity=34.5070422535211, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=260, Percent_Identity=25.7692307692308, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=136, Percent_Identity=36.0294117647059, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=135, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=467, Percent_Identity=30.406852248394, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6319685, Length=400, Percent_Identity=26.25, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648113, Length=444, Percent_Identity=29.954954954955, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24644968, Length=401, Percent_Identity=23.6907730673317, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24652985, Length=397, Percent_Identity=24.6851385390428, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922090, Length=397, Percent_Identity=24.6851385390428, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652981, Length=397, Percent_Identity=24.6851385390428, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652983, Length=397, Percent_Identity=24.6851385390428, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648435, Length=395, Percent_Identity=24.5569620253165, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648437, Length=395, Percent_Identity=24.5569620253165, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550774, Length=395, Percent_Identity=24.5569620253165, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648441, Length=395, Percent_Identity=24.5569620253165, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648439, Length=395, Percent_Identity=24.5569620253165, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356731, Length=128, Percent_Identity=38.28125, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 43703; Mature: 43703

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMSLEELNTKYNIFITISRMKYRDKGKLRDLKFGIKDIILTKGVRTTAGSKILKDFIPS
CCCCHHHCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
ENAYIVDKILAEGGEIIGKTNTHEFAIGATNTSSLIGPAKNPYDPERISGGSSGGSAVAV
CCCCHHHHHHHCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHH
ALGLVDVGIGTDTGGSVRIPASLCGVIGFKPTYGIIPTDGVIPFSWTFDTIGFLAKDISL
HHHHHEECCCCCCCCCEECCHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHH
IRRTIEALAENKIPFISYSKRRPTLGLFLFGDYETSKALEPVINKLSSYFDLVQIENELL
HHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IKHSRIVRKTIALAEASSYHMKYYDKYKDLYSSDVRKLIEEGFKILATDYVNSLRIRKVL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEYFSIFKKIDALISPTTRIVAPKIQEVIGNELKYRDSLIANTEIFNTLGAPSISIPVT
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHCCCCCEEEEEE
KLNDLPVGLMISGEPYKDGTVLDIAEEIMGIVGFIK
ECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKMSLEELNTKYNIFITISRMKYRDKGKLRDLKFGIKDIILTKGVRTTAGSKILKDFIPS
CCCCHHHCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
ENAYIVDKILAEGGEIIGKTNTHEFAIGATNTSSLIGPAKNPYDPERISGGSSGGSAVAV
CCCCHHHHHHHCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHH
ALGLVDVGIGTDTGGSVRIPASLCGVIGFKPTYGIIPTDGVIPFSWTFDTIGFLAKDISL
HHHHHEECCCCCCCCCEECCHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHH
IRRTIEALAENKIPFISYSKRRPTLGLFLFGDYETSKALEPVINKLSSYFDLVQIENELL
HHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IKHSRIVRKTIALAEASSYHMKYYDKYKDLYSSDVRKLIEEGFKILATDYVNSLRIRKVL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEYFSIFKKIDALISPTTRIVAPKIQEVIGNELKYRDSLIANTEIFNTLGAPSISIPVT
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHCCCCCEEEEEE
KLNDLPVGLMISGEPYKDGTVLDIAEEIMGIVGFIK
ECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA