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The map label for this gene is glyS [H]

Identifier: 161723838

GI number: 161723838

Start: 1219241

End: 1221235

Strand: Reverse

Name: glyS [H]

Synonym: RC1316

Alternate gene names: 161723838

Gene position: 1221235-1219241 (Counterclockwise)

Preceding gene: 15893240

Following gene: 15893238

Centisome position: 96.25

GC content: 31.93

Gene sequence:

>1995_bases
GTGAGCGAGTTATTATTAGAACTATTTAGCGAAGAAATTCCGGCTTTTATGCAAAAAAATGCTGAAGAGGGTTATTTAAA
TATATTCACGAAAATTTTTGAAGAAAACGAAATATTTGCAAAAGTACAAGTATTCGTGGGACCTCGCAGGATAACGCTGC
ATGCTACCCATTTGCCAAAGATAACTTTGCCAAAAGAGGAAGAAATTAAAGGACCAAGCATAGAAGCTCCGGAAGCCGCT
ATTAACGGCTTTTGTAAAGCTCATAATGTTAGTAAATTAGAGCTTCCTACTAAATTAATTAGTAATCAGCTATATTATTT
CTTTGTCAAAAAAACAGAAGAAAGAGAAATAAAAGAGATTTTGCCGGAGATTATTATAGAGGCTATTAATAAATATAGCT
GGGCAAAATCGATGTTTTGGGGTGATTACAAAATAAAATGGATTAGACCTCTGCGAAATATTTTATGCATATTTGACGGC
GAAATATTACCGATGCAATTTGGGCATTTAACCGCTAATAATATTACGTATGGGCATCGTCTTACTGATAATAAAAAATT
AGAAGTAACAGATTTTGAAGATTATAGAAATAAGCTTTTAGAAAATCATGTTATTTTAGAAAGAGCAAAGAGAGAGGCAA
TAATTAAAACCGGTTTACTGGAACTAGCAAGTTCTCATGAGTTAATTATAAAAGAAGATAATCGTTTAGTAGAAGAAGTA
GTAGGGCTTAGCGAATTTCCTGTTGTGCTGCTTGGAAAAATACCGCAAAAATTTTTAGAGTTACCTAAAGAAGTACTTAT
TTCTTCGATGCGTACGCATCAGAAATATTTTTGTTTATTTGATAAAACAGGAAATTTCACACCATATTTTCTCTTTGTCA
GTAATGGCAGATTTACAAATGCTGAACTAGTTATTCAAGGTAACGAGAAAGTTCTATCGGCACGTCTTTCGGATGCTTTA
TATTTTTGTAAGCAGGATATAGCTAAAACTTTAGAATCAAGATTAGGCCAATTAGAAGCTGTAACATTTCATGCAAAGCT
TGGTAATTTAAGAGAAAAAATTGAACGCATAACCGATATTTGTAATTATATAGCTCCAAATAATAAGGATTTGATTACAG
CAGCTAGACTTTGCAAAAGTGATCTTGTTTCTGAGATGGTCGGGGAATTTCCTGATTTACAGGGGATTATGGGTTATTAT
TATGCAAAACATGAAGGGCTAAATGCAGAAATAGCCGCTGCAATTAGAGACCATTATAAACCGCAAGGTTTGAGCGACAA
TTTACCAAGTGGAAATGCGGCTTTACTTGCTTTAGCCGATAAGCTAGATAGTTTAGTAGGTTTGATGATAGCAGGGGAAA
CACCAACAGGCTCAGGTGATCCATATGCCTTAAGACGTCAAGCTCTTGGTATAATAAGAATAATACTTGAAAATAAATTA
GAGCTAAATTTTAATGATTTAATTAATTTTTCTATAAACTTATATAAAGATTCATCTGACGAAAATAAGAATTTAATAAT
ATCTTTTTTTGAAGAAAGAGCAAAATTTTACTTTAAAAATGACTATGATATTGCGTTAATTAATGCAGTTCTTGACTTAA
ATTTAGTAGATACAAACTTTAAGCTTGATGCGTTAAAAGAATTTTTAATAGAAGACGCAGGCAAGCAATTATTAAATGCT
TACAAACGAGCTAGCAATATAATTGGTGATCAGAAAATTACCGGCTTAGTTGATGCGAGTCTCTTTAGTACACAACCTGA
AAAAGAATTATTTGAAGTAATCCAAAAAATTTCTCCGCAAATTATAGATAGTATAGCTGATAAAGATTATAAGAAAGCAT
TAAATTTATTATCGTCTCTATTAACTCCCATTACTAGCTTTTTTGATAATGTACTCGTTAATGACTCTGATCCAAAGATC
GCTCAAAATCGTTTGTCGTTATTACAGAATATTTGTGAATTATTTGATAAAGTAGCTAAGTTTAACCGCTTATGA

Upstream 100 bases:

>100_bases
ATGCACTTGGAGTAATCAGCGTCACTGAGCGTGCTTCGTATGTTTTAAGAGTGCGTCATTTAGCTAGAATTTGTTGTACA
AAATGGTTGGAGATGAACAA

Downstream 100 bases:

>100_bases
TAAACAAAGCAGTTCAATTTATAAAATCAGGTAAAGTGGTTGTTTTTCCGACTGAAACAGTCTATGGGATTGGTGCGGAT
GCCACAAATAATGAGGCGTG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 664; Mature: 663

Protein sequence:

>664_residues
MSELLLELFSEEIPAFMQKNAEEGYLNIFTKIFEENEIFAKVQVFVGPRRITLHATHLPKITLPKEEEIKGPSIEAPEAA
INGFCKAHNVSKLELPTKLISNQLYYFFVKKTEEREIKEILPEIIIEAINKYSWAKSMFWGDYKIKWIRPLRNILCIFDG
EILPMQFGHLTANNITYGHRLTDNKKLEVTDFEDYRNKLLENHVILERAKREAIIKTGLLELASSHELIIKEDNRLVEEV
VGLSEFPVVLLGKIPQKFLELPKEVLISSMRTHQKYFCLFDKTGNFTPYFLFVSNGRFTNAELVIQGNEKVLSARLSDAL
YFCKQDIAKTLESRLGQLEAVTFHAKLGNLREKIERITDICNYIAPNNKDLITAARLCKSDLVSEMVGEFPDLQGIMGYY
YAKHEGLNAEIAAAIRDHYKPQGLSDNLPSGNAALLALADKLDSLVGLMIAGETPTGSGDPYALRRQALGIIRIILENKL
ELNFNDLINFSINLYKDSSDENKNLIISFFEERAKFYFKNDYDIALINAVLDLNLVDTNFKLDALKEFLIEDAGKQLLNA
YKRASNIIGDQKITGLVDASLFSTQPEKELFEVIQKISPQIIDSIADKDYKKALNLLSSLLTPITSFFDNVLVNDSDPKI
AQNRLSLLQNICELFDKVAKFNRL

Sequences:

>Translated_664_residues
MSELLLELFSEEIPAFMQKNAEEGYLNIFTKIFEENEIFAKVQVFVGPRRITLHATHLPKITLPKEEEIKGPSIEAPEAA
INGFCKAHNVSKLELPTKLISNQLYYFFVKKTEEREIKEILPEIIIEAINKYSWAKSMFWGDYKIKWIRPLRNILCIFDG
EILPMQFGHLTANNITYGHRLTDNKKLEVTDFEDYRNKLLENHVILERAKREAIIKTGLLELASSHELIIKEDNRLVEEV
VGLSEFPVVLLGKIPQKFLELPKEVLISSMRTHQKYFCLFDKTGNFTPYFLFVSNGRFTNAELVIQGNEKVLSARLSDAL
YFCKQDIAKTLESRLGQLEAVTFHAKLGNLREKIERITDICNYIAPNNKDLITAARLCKSDLVSEMVGEFPDLQGIMGYY
YAKHEGLNAEIAAAIRDHYKPQGLSDNLPSGNAALLALADKLDSLVGLMIAGETPTGSGDPYALRRQALGIIRIILENKL
ELNFNDLINFSINLYKDSSDENKNLIISFFEERAKFYFKNDYDIALINAVLDLNLVDTNFKLDALKEFLIEDAGKQLLNA
YKRASNIIGDQKITGLVDASLFSTQPEKELFEVIQKISPQIIDSIADKDYKKALNLLSSLLTPITSFFDNVLVNDSDPKI
AQNRLSLLQNICELFDKVAKFNRL
>Mature_663_residues
SELLLELFSEEIPAFMQKNAEEGYLNIFTKIFEENEIFAKVQVFVGPRRITLHATHLPKITLPKEEEIKGPSIEAPEAAI
NGFCKAHNVSKLELPTKLISNQLYYFFVKKTEEREIKEILPEIIIEAINKYSWAKSMFWGDYKIKWIRPLRNILCIFDGE
ILPMQFGHLTANNITYGHRLTDNKKLEVTDFEDYRNKLLENHVILERAKREAIIKTGLLELASSHELIIKEDNRLVEEVV
GLSEFPVVLLGKIPQKFLELPKEVLISSMRTHQKYFCLFDKTGNFTPYFLFVSNGRFTNAELVIQGNEKVLSARLSDALY
FCKQDIAKTLESRLGQLEAVTFHAKLGNLREKIERITDICNYIAPNNKDLITAARLCKSDLVSEMVGEFPDLQGIMGYYY
AKHEGLNAEIAAAIRDHYKPQGLSDNLPSGNAALLALADKLDSLVGLMIAGETPTGSGDPYALRRQALGIIRIILENKLE
LNFNDLINFSINLYKDSSDENKNLIISFFEERAKFYFKNDYDIALINAVLDLNLVDTNFKLDALKEFLIEDAGKQLLNAY
KRASNIIGDQKITGLVDASLFSTQPEKELFEVIQKISPQIIDSIADKDYKKALNLLSSLLTPITSFFDNVLVNDSDPKIA
QNRLSLLQNICELFDKVAKFNRL

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=690, Percent_Identity=31.7391304347826, Blast_Score=324, Evalue=1e-89,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75586; Mature: 75455

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELLLELFSEEIPAFMQKNAEEGYLNIFTKIFEENEIFAKVQVFVGPRRITLHATHLPK
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEEECCEEEEEEEECCCC
ITLPKEEEIKGPSIEAPEAAINGFCKAHNVSKLELPTKLISNQLYYFFVKKTEEREIKEI
EECCCHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHH
LPEIIIEAINKYSWAKSMFWGDYKIKWIRPLRNILCIFDGEILPMQFGHLTANNITYGHR
HHHHHHHHHHHHHHHHHHEECCEEEHHHHHHHHHEEHCCCCEEEEECCCEEECCEEECCE
LTDNKKLEVTDFEDYRNKLLENHVILERAKREAIIKTGLLELASSHELIIKEDNRLVEEV
ECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
VGLSEFPVVLLGKIPQKFLELPKEVLISSMRTHQKYFCLFDKTGNFTPYFLFVSNGRFTN
HCCCCCCHHHHHHCCHHHHHCCHHHHHHHHHHCCEEEEEEECCCCCCEEEEEEECCCEEC
AELVIQGNEKVLSARLSDALYFCKQDIAKTLESRLGQLEAVTFHAKLGNLREKIERITDI
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHH
CNYIAPNNKDLITAARLCKSDLVSEMVGEFPDLQGIMGYYYAKHEGLNAEIAAAIRDHYK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCC
PQGLSDNLPSGNAALLALADKLDSLVGLMIAGETPTGSGDPYALRRQALGIIRIILENKL
CCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCC
ELNFNDLINFSINLYKDSSDENKNLIISFFEERAKFYFKNDYDIALINAVLDLNLVDTNF
CCCHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHHEEECCCCCEEEEEHHHEEEEECCCC
KLDALKEFLIEDAGKQLLNAYKRASNIIGDQKITGLVDASLFSTQPEKELFEVIQKISPQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHH
IIDSIADKDYKKALNLLSSLLTPITSFFDNVLVNDSDPKIAQNRLSLLQNICELFDKVAK
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHH
FNRL
HCCC
>Mature Secondary Structure 
SELLLELFSEEIPAFMQKNAEEGYLNIFTKIFEENEIFAKVQVFVGPRRITLHATHLPK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEEECCEEEEEEEECCCC
ITLPKEEEIKGPSIEAPEAAINGFCKAHNVSKLELPTKLISNQLYYFFVKKTEEREIKEI
EECCCHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHH
LPEIIIEAINKYSWAKSMFWGDYKIKWIRPLRNILCIFDGEILPMQFGHLTANNITYGHR
HHHHHHHHHHHHHHHHHHEECCEEEHHHHHHHHHEEHCCCCEEEEECCCEEECCEEECCE
LTDNKKLEVTDFEDYRNKLLENHVILERAKREAIIKTGLLELASSHELIIKEDNRLVEEV
ECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
VGLSEFPVVLLGKIPQKFLELPKEVLISSMRTHQKYFCLFDKTGNFTPYFLFVSNGRFTN
HCCCCCCHHHHHHCCHHHHHCCHHHHHHHHHHCCEEEEEEECCCCCCEEEEEEECCCEEC
AELVIQGNEKVLSARLSDALYFCKQDIAKTLESRLGQLEAVTFHAKLGNLREKIERITDI
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHH
CNYIAPNNKDLITAARLCKSDLVSEMVGEFPDLQGIMGYYYAKHEGLNAEIAAAIRDHYK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCC
PQGLSDNLPSGNAALLALADKLDSLVGLMIAGETPTGSGDPYALRRQALGIIRIILENKL
CCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCC
ELNFNDLINFSINLYKDSSDENKNLIISFFEERAKFYFKNDYDIALINAVLDLNLVDTNF
CCCHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHHEEECCCCCEEEEEHHHEEEEECCCC
KLDALKEFLIEDAGKQLLNAYKRASNIIGDQKITGLVDASLFSTQPEKELFEVIQKISPQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHH
IIDSIADKDYKKALNLLSSLLTPITSFFDNVLVNDSDPKIAQNRLSLLQNICELFDKVAK
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHH
FNRL
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA