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The map label for this gene is gltA [H]

Identifier: 15893231

GI number: 15893231

Start: 1211392

End: 1212699

Strand: Reverse

Name: gltA [H]

Synonym: RC1308

Alternate gene names: 15893231

Gene position: 1212699-1211392 (Counterclockwise)

Preceding gene: 15893232

Following gene: 161723839

Centisome position: 95.58

GC content: 36.54

Gene sequence:

>1308_bases
ATGACCAATGAAAATAATAATGATTCAGAATTTGCTGAATTAAAAATCAGAGGAAAAATATTTAAATTACCTATACTTAA
AGCAAGTATCGGTGAGGATGTAATCGATATAAGTAGGGTATCTGCGGAAGCCGATTGCTTTACTTACGACCCGGGTTTTA
TGTCTACTGCTTCTTGTCAGTCTACTATCACCTATATAGACGGTGATAAAGGAATCTTGCGGCATCGAGGATATGATATT
AAAGACTTAGCTGAGAAAAGTGATTTTTTAGAAGTGGCATATTTACTGATTTATGGGGAACTACCAAGTGGCGAGCAGTA
TAATAATTTCACTAAACAGGTTGCTCATCATTCATTAGTGAATGAAAGATTACACTATTTATTTCAAACCTTTTGTAGCT
CTTCTCATCCTATGGCTATTATGCTTGCGGCTGTCGGTTCTCTTTCGGCATTTTATCCTGATTTATTGAATTTTAAGGAA
GCAGATTACGAACTTACCGCTATTAGAATGATTGCTAAGATACCTACCATCGCTGCAATGTCTTATAAATATTCTATAGG
ACAACCGTTTATTTATCCTGATAATTCGTTAGATTTTACCGAAAATTTTCTGCATATGATGTTTGCAACGCCTTGTACGA
AATATACAGTAAATCCAATAATAAAAAATGCTCTTAATAAGATATTTATCCTACATGCCGATCATGAGCAGAATGCTTCT
ACTTCAACAGTCCGAATTGCTGGCTCATCCGGAGCTAACCCTTTTGCTTGTATTAGCACGGGTATTGCCTCACTTTGGGG
ACCTGCTCACGGCGGGGCTAATGAAGCGGTAATAAATATGATTAAAGAAATCGGTAGTTCTGAGTATATTCCTAAATATA
TAGCTAAAGCTAAGGATAAAAATGATCCATTTAGATTAATGGGTTTTGGTCATCGTGTATATAAAAACTATGACCCGCGT
GCCGCAGTACTTAAAGAAACGTGCAAAGAAGTATTAAAGGAACTCGGGCAGCTAGACAACAATCCGTTCTTACAAATAGC
AATAGAACTTGAAGCTATCGCTCTTAAAGATGAATATTTTATTGAGAGAAAATTATATCCAAATGTTGATTTTTATTCGG
GTATTATCTATAAAGCTATGGGTATACCGTCGCAAATGTTCACGGTACTTTTTGCAATAGCAAGAACCGTAGGCTGGATG
GCACAATGGAAAGAAATGCACGAAGACCCTGAACAAAAAATCAGCAGACCTAGACAGCTTTACACCGGTTATGTACATAG
AGAGTATAAGGGTATTCGGGAGAGGTAA

Upstream 100 bases:

>100_bases
TATCAAAGAAAATTTTTAAATCCTAATAAATCATAATGCATTTCTAATTTCATTAAATTAGGGTTATACTTACTGATGTA
TAAAAATAAGGTATATCCAT

Downstream 100 bases:

>100_bases
ACATTTTCGTCATTGCGAGCGACTGCAAGGAGCGTAGCAATCTCAAGAGATTTGATGAGATTTCCACGTCGCTTCGCGCC
TTGTAATGACGTTGAAAATA

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTNENNNDSEFAELKIRGKIFKLPILKASIGEDVIDISRVSAEADCFTYDPGFMSTASCQSTITYIDGDKGILRHRGYDI
KDLAEKSDFLEVAYLLIYGELPSGEQYNNFTKQVAHHSLVNERLHYLFQTFCSSSHPMAIMLAAVGSLSAFYPDLLNFKE
ADYELTAIRMIAKIPTIAAMSYKYSIGQPFIYPDNSLDFTENFLHMMFATPCTKYTVNPIIKNALNKIFILHADHEQNAS
TSTVRIAGSSGANPFACISTGIASLWGPAHGGANEAVINMIKEIGSSEYIPKYIAKAKDKNDPFRLMGFGHRVYKNYDPR
AAVLKETCKEVLKELGQLDNNPFLQIAIELEAIALKDEYFIERKLYPNVDFYSGIIYKAMGIPSQMFTVLFAIARTVGWM
AQWKEMHEDPEQKISRPRQLYTGYVHREYKGIRER

Sequences:

>Translated_435_residues
MTNENNNDSEFAELKIRGKIFKLPILKASIGEDVIDISRVSAEADCFTYDPGFMSTASCQSTITYIDGDKGILRHRGYDI
KDLAEKSDFLEVAYLLIYGELPSGEQYNNFTKQVAHHSLVNERLHYLFQTFCSSSHPMAIMLAAVGSLSAFYPDLLNFKE
ADYELTAIRMIAKIPTIAAMSYKYSIGQPFIYPDNSLDFTENFLHMMFATPCTKYTVNPIIKNALNKIFILHADHEQNAS
TSTVRIAGSSGANPFACISTGIASLWGPAHGGANEAVINMIKEIGSSEYIPKYIAKAKDKNDPFRLMGFGHRVYKNYDPR
AAVLKETCKEVLKELGQLDNNPFLQIAIELEAIALKDEYFIERKLYPNVDFYSGIIYKAMGIPSQMFTVLFAIARTVGWM
AQWKEMHEDPEQKISRPRQLYTGYVHREYKGIRER
>Mature_434_residues
TNENNNDSEFAELKIRGKIFKLPILKASIGEDVIDISRVSAEADCFTYDPGFMSTASCQSTITYIDGDKGILRHRGYDIK
DLAEKSDFLEVAYLLIYGELPSGEQYNNFTKQVAHHSLVNERLHYLFQTFCSSSHPMAIMLAAVGSLSAFYPDLLNFKEA
DYELTAIRMIAKIPTIAAMSYKYSIGQPFIYPDNSLDFTENFLHMMFATPCTKYTVNPIIKNALNKIFILHADHEQNAST
STVRIAGSSGANPFACISTGIASLWGPAHGGANEAVINMIKEIGSSEYIPKYIAKAKDKNDPFRLMGFGHRVYKNYDPRA
AVLKETCKEVLKELGQLDNNPFLQIAIELEAIALKDEYFIERKLYPNVDFYSGIIYKAMGIPSQMFTVLFAIARTVGWMA
QWKEMHEDPEQKISRPRQLYTGYVHREYKGIRER

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=393, Percent_Identity=28.2442748091603, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1786939, Length=421, Percent_Identity=61.7577197149644, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1786527, Length=365, Percent_Identity=31.2328767123288, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17555174, Length=382, Percent_Identity=26.7015706806283, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324328, Length=368, Percent_Identity=26.3586956521739, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325257, Length=402, Percent_Identity=27.6119402985075, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319850, Length=370, Percent_Identity=25.6756756756757, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24640126, Length=405, Percent_Identity=27.6543209876543, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24640124, Length=405, Percent_Identity=27.6543209876543, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21356863, Length=394, Percent_Identity=25.8883248730964, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 49211; Mature: 49080

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNENNNDSEFAELKIRGKIFKLPILKASIGEDVIDISRVSAEADCFTYDPGFMSTASCQ
CCCCCCCCCCEEEEEEECEEEEEEEHHHHCCCCHHHHHHHCCCCCEEEECCCCCCCCCCC
STITYIDGDKGILRHRGYDIKDLAEKSDFLEVAYLLIYGELPSGEQYNNFTKQVAHHSLV
CEEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
NERLHYLFQTFCSSSHPMAIMLAAVGSLSAFYPDLLNFKEADYELTAIRMIAKIPTIAAM
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHE
SYKYSIGQPFIYPDNSLDFTENFLHMMFATPCTKYTVNPIIKNALNKIFILHADHEQNAS
EEEEECCCCEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEEEECCCCCCCC
TSTVRIAGSSGANPFACISTGIASLWGPAHGGANEAVINMIKEIGSSEYIPKYIAKAKDK
CCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCC
NDPFRLMGFGHRVYKNYDPRAAVLKETCKEVLKELGQLDNNPFLQIAIELEAIALKDEYF
CCCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECHHHH
IERKLYPNVDFYSGIIYKAMGIPSQMFTVLFAIARTVGWMAQWKEMHEDPEQKISRPRQL
HHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHH
YTGYVHREYKGIRER
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNENNNDSEFAELKIRGKIFKLPILKASIGEDVIDISRVSAEADCFTYDPGFMSTASCQ
CCCCCCCCCEEEEEEECEEEEEEEHHHHCCCCHHHHHHHCCCCCEEEECCCCCCCCCCC
STITYIDGDKGILRHRGYDIKDLAEKSDFLEVAYLLIYGELPSGEQYNNFTKQVAHHSLV
CEEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
NERLHYLFQTFCSSSHPMAIMLAAVGSLSAFYPDLLNFKEADYELTAIRMIAKIPTIAAM
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHE
SYKYSIGQPFIYPDNSLDFTENFLHMMFATPCTKYTVNPIIKNALNKIFILHADHEQNAS
EEEEECCCCEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEEEECCCCCCCC
TSTVRIAGSSGANPFACISTGIASLWGPAHGGANEAVINMIKEIGSSEYIPKYIAKAKDK
CCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCC
NDPFRLMGFGHRVYKNYDPRAAVLKETCKEVLKELGQLDNNPFLQIAIELEAIALKDEYF
CCCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECHHHH
IERKLYPNVDFYSGIIYKAMGIPSQMFTVLFAIARTVGWMAQWKEMHEDPEQKISRPRQL
HHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHH
YTGYVHREYKGIRER
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3112124; 9823893; 7557459 [H]