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The map label for this gene is mrcA

Identifier: 15893168

GI number: 15893168

Start: 1150700

End: 1153072

Strand: Reverse

Name: mrcA

Synonym: RC1245

Alternate gene names: 15893168

Gene position: 1153072-1150700 (Counterclockwise)

Preceding gene: 15893169

Following gene: 15893167

Centisome position: 90.88

GC content: 36.28

Gene sequence:

>2373_bases
ATGTATAAATCTCTTCTTTTTTGTTTAAAAATTTTTGTCTTCCTTATTTTAGTAGGTTGCGGGATTACTGCTTATATAAT
ATACCACTATTCACGTGATTTGCCTGATTACTCACAGCTTGCACGTTATTATCCGCCGTCGGTAACGCGTATTTATTCCC
GTGACGGCAAATTAATGGAGGAGTACGCTTTTGAACGTCGTGTATTTGTACCGATTAATAGCGTACCGAGTTCGCTAATA
GAAAGTTTTATTGCAGCTGAGGATAAGAATTTCTATAACCATCCCGGCGTTGATTTGTTTGGAATAGTTAGAGCGGCATT
TTTAAACATATCTAATTATTTACATCATAGGCGTATGGAAGGAGCGTCTACCATTACTCAGCAAGTGGTTAAAAATTTTC
TGCTAACTAACGAAGTTTCTCTTGAACGTAAGATTAAAGAAGCGATTCTTTCTTATATGATTTCAAGAGTATTTACTAAG
GATCAAATTTTAGAATTATATCTTAATCAAACATTTTTTGGTCGTGGTGCATACGGTGTTGCGGTAGCTGCACAAAATTA
TTTTAATAAATCCGTAGAGGAACTTACTATTGCAGAATCAGCTTTTATTGCGGCACTGCCTAAAGCTCCTTCCGAACTTA
ATCCTGAGAGAAATTATGCTAGAGTAAAAGCTCGTCGTGATTACGTAATAACACGTATGTTTGAAGATGGTTATATAACA
AGAGACGCTGCGAAAGAAGCTATGGATAGCCCGATAGTTTTACGTAAACGAGCTAAGGAAGAAACTGTTACGGCAGATTA
TTATGCTGCACAGGTCAGAGAAGAAGTAATTAGGATGTTAAATAGTAAGGAAGTATTTTATACCGGAGGTCTTACTATCA
TTACTTCTTTAGATGCAAAGATGCAGCAATTAGCTGAAAACTCTCTAAGAAAAGGTCTAAGGGAGTTTGATAGAAGGTGT
GGTTTTAGAAAGCCTATAGCTAATATTTCTCTTGATAATTGGCAAGGAGAGCTAAAAAAACTACCGACTCCTCCATCGCT
TTTAGAGTATAAATTAGCTGTAGTTCTAGATGTAGCCGATAATCATGTAGAGATTGGACTTATAGACGGCAGCAAATCTA
AGATGCCTATTGCTGAAATGAAATGGGCAAGAAGTAATTTTAAATCAGTTAAGACCTTGCTTAAAAAAGGTGATGTGATA
GTGGTTGAGGCTATAAAAGAGGGGTATGCTCTTCGTCAGATTCCTGAAGTAAACGGTGCTATTATGGTTATGAACCCAAA
TACCGGACAGGTACTTGCAAGCGTCGGCGGTTATGATTTTTCCACAAGTAAATTTGATAGAGTAACTCAAGCACTGCGTC
AACCCGGTTCTTTGAGTAAAACCTTTGTTTATTTAGCAGCTCTTGAAAACGGTGTTAAGCCGAATCAGATTTTTAATGAT
GGACCTATTGAGATTTCGCAAGGTCCAGGGATGCCTAGTTGGCGTCCTAAAAATTATGAGGGTAAGTTTTTAGGTGAAAT
CACTATGCGTACTGGTCTTGAAAAATCTCGTAATCTTATAACCGTAAGAGTTGCAACTGCCGTAGGACTTACAAAAATCG
TTGATATAATTAAGCGTTTCGGTATTAATAATGAACCAAAAAAAGTTTACTCTATGGTACTTGGTTCCATTGAAACCACA
CTTAGCAGAATGACTAATGCTTATGCAATTATTGCTAACGGCGGTAAAAAAGTTGAGCCTCATTTTGTAGAATTAATTAA
AGATCGTAACGGTAAAATTATTTATAGGCGAGATGATAGAGAATGTCTTGCTTGTAATGTTTCGGATAGTAATTTAGATA
CCGCAATATTAGAAATACCGAAAGAATATATCTATAGAGTTACGGATGAAGCTAGTGATTATCAAATTACTTCGTTTTTA
ACCGGAGCAATAGATAGAGGTACCGGTTATGCTGCGAAGAAGTTAGGGAAAATTATCGGTGGAAAAACCGGTACTAGTAA
CGATAGTAAAGATACATGGTTTGTAGGTTTTACACCCAAAATAGTAGTCGGCAGTTATGTGGGTTATGATACACCGAAAG
AGCTTGGAAAAAGAGCAACAGGCTCAAACGTAGTATTACCGATTTTTATTGATTTTATGAGTAATGCTTATAAGGATAAG
CCATCGCTGCCTTTCAAAGTTCCAGATTCAATTAAGCTAATCGCCGTAGATAGCGCAACTGGTAAAATTACACCAGGCGG
TACTGTTATAGAAGCATTTAAAGTAAATAATGTTCAGATGCTTGAGAATGAAGATATGATTGATAATCAAGATAATAATG
ATATTTTTGATTATGTACCGAGTAAGGAAGATCAATCGCAGGAAATTTACTAA

Upstream 100 bases:

>100_bases
ATATATCCAAAACCCTAATAAATCTTTACTTGGTAAAATCAGTGATGTAAAAATTACTAAGGCTTCACTTAATAGTTTAA
CCGGCGAAATATTATAAGTC

Downstream 100 bases:

>100_bases
ATTGTATAATTAGTTTAAGCCGATGTCATTCCCTGTTCTCTATGTCATGCAACAAAACCACCTATTTTTATTTGAACCTA
TGAAAAAAATTATTTTATAT

Product: penicillin-binding protein 1a

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase

Number of amino acids: Translated: 790; Mature: 790

Protein sequence:

>790_residues
MYKSLLFCLKIFVFLILVGCGITAYIIYHYSRDLPDYSQLARYYPPSVTRIYSRDGKLMEEYAFERRVFVPINSVPSSLI
ESFIAAEDKNFYNHPGVDLFGIVRAAFLNISNYLHHRRMEGASTITQQVVKNFLLTNEVSLERKIKEAILSYMISRVFTK
DQILELYLNQTFFGRGAYGVAVAAQNYFNKSVEELTIAESAFIAALPKAPSELNPERNYARVKARRDYVITRMFEDGYIT
RDAAKEAMDSPIVLRKRAKEETVTADYYAAQVREEVIRMLNSKEVFYTGGLTIITSLDAKMQQLAENSLRKGLREFDRRC
GFRKPIANISLDNWQGELKKLPTPPSLLEYKLAVVLDVADNHVEIGLIDGSKSKMPIAEMKWARSNFKSVKTLLKKGDVI
VVEAIKEGYALRQIPEVNGAIMVMNPNTGQVLASVGGYDFSTSKFDRVTQALRQPGSLSKTFVYLAALENGVKPNQIFND
GPIEISQGPGMPSWRPKNYEGKFLGEITMRTGLEKSRNLITVRVATAVGLTKIVDIIKRFGINNEPKKVYSMVLGSIETT
LSRMTNAYAIIANGGKKVEPHFVELIKDRNGKIIYRRDDRECLACNVSDSNLDTAILEIPKEYIYRVTDEASDYQITSFL
TGAIDRGTGYAAKKLGKIIGGKTGTSNDSKDTWFVGFTPKIVVGSYVGYDTPKELGKRATGSNVVLPIFIDFMSNAYKDK
PSLPFKVPDSIKLIAVDSATGKITPGGTVIEAFKVNNVQMLENEDMIDNQDNNDIFDYVPSKEDQSQEIY

Sequences:

>Translated_790_residues
MYKSLLFCLKIFVFLILVGCGITAYIIYHYSRDLPDYSQLARYYPPSVTRIYSRDGKLMEEYAFERRVFVPINSVPSSLI
ESFIAAEDKNFYNHPGVDLFGIVRAAFLNISNYLHHRRMEGASTITQQVVKNFLLTNEVSLERKIKEAILSYMISRVFTK
DQILELYLNQTFFGRGAYGVAVAAQNYFNKSVEELTIAESAFIAALPKAPSELNPERNYARVKARRDYVITRMFEDGYIT
RDAAKEAMDSPIVLRKRAKEETVTADYYAAQVREEVIRMLNSKEVFYTGGLTIITSLDAKMQQLAENSLRKGLREFDRRC
GFRKPIANISLDNWQGELKKLPTPPSLLEYKLAVVLDVADNHVEIGLIDGSKSKMPIAEMKWARSNFKSVKTLLKKGDVI
VVEAIKEGYALRQIPEVNGAIMVMNPNTGQVLASVGGYDFSTSKFDRVTQALRQPGSLSKTFVYLAALENGVKPNQIFND
GPIEISQGPGMPSWRPKNYEGKFLGEITMRTGLEKSRNLITVRVATAVGLTKIVDIIKRFGINNEPKKVYSMVLGSIETT
LSRMTNAYAIIANGGKKVEPHFVELIKDRNGKIIYRRDDRECLACNVSDSNLDTAILEIPKEYIYRVTDEASDYQITSFL
TGAIDRGTGYAAKKLGKIIGGKTGTSNDSKDTWFVGFTPKIVVGSYVGYDTPKELGKRATGSNVVLPIFIDFMSNAYKDK
PSLPFKVPDSIKLIAVDSATGKITPGGTVIEAFKVNNVQMLENEDMIDNQDNNDIFDYVPSKEDQSQEIY
>Mature_790_residues
MYKSLLFCLKIFVFLILVGCGITAYIIYHYSRDLPDYSQLARYYPPSVTRIYSRDGKLMEEYAFERRVFVPINSVPSSLI
ESFIAAEDKNFYNHPGVDLFGIVRAAFLNISNYLHHRRMEGASTITQQVVKNFLLTNEVSLERKIKEAILSYMISRVFTK
DQILELYLNQTFFGRGAYGVAVAAQNYFNKSVEELTIAESAFIAALPKAPSELNPERNYARVKARRDYVITRMFEDGYIT
RDAAKEAMDSPIVLRKRAKEETVTADYYAAQVREEVIRMLNSKEVFYTGGLTIITSLDAKMQQLAENSLRKGLREFDRRC
GFRKPIANISLDNWQGELKKLPTPPSLLEYKLAVVLDVADNHVEIGLIDGSKSKMPIAEMKWARSNFKSVKTLLKKGDVI
VVEAIKEGYALRQIPEVNGAIMVMNPNTGQVLASVGGYDFSTSKFDRVTQALRQPGSLSKTFVYLAALENGVKPNQIFND
GPIEISQGPGMPSWRPKNYEGKFLGEITMRTGLEKSRNLITVRVATAVGLTKIVDIIKRFGINNEPKKVYSMVLGSIETT
LSRMTNAYAIIANGGKKVEPHFVELIKDRNGKIIYRRDDRECLACNVSDSNLDTAILEIPKEYIYRVTDEASDYQITSFL
TGAIDRGTGYAAKKLGKIIGGKTGTSNDSKDTWFVGFTPKIVVGSYVGYDTPKELGKRATGSNVVLPIFIDFMSNAYKDK
PSLPFKVPDSIKLIAVDSATGKITPGGTVIEAFKVNNVQMLENEDMIDNQDNNDIFDYVPSKEDQSQEIY

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI87082258, Length=858, Percent_Identity=33.2167832167832, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1786343, Length=305, Percent_Identity=29.8360655737705, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788867, Length=260, Percent_Identity=30, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789601, Length=156, Percent_Identity=38.4615384615385, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBPA_RICCN (Q92G78)

Other databases:

- EMBL:   AE006914
- PIR:   E97855
- RefSeq:   NP_360882.1
- ProteinModelPortal:   Q92G78
- SMR:   Q92G78
- GeneID:   928398
- GenomeReviews:   AE006914_GR
- KEGG:   rco:RC1245
- NMPDR:   fig|272944.1.peg.1245
- OMA:   NSKEEFY
- ProtClustDB:   CLSK871084
- BioCyc:   RCON272944:RC1245-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460
- TIGRFAMs:   TIGR02074

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase; SSF56601 PBP_transp_fold

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 88685; Mature: 88685

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: ACT_SITE 457-457

Signals:

None

Transmembrane regions:

HASH(0xe34749c)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKSLLFCLKIFVFLILVGCGITAYIIYHYSRDLPDYSQLARYYPPSVTRIYSRDGKLME
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHCCCCHHHH
EYAFERRVFVPINSVPSSLIESFIAAEDKNFYNHPGVDLFGIVRAAFLNISNYLHHRRME
HHHHCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GASTITQQVVKNFLLTNEVSLERKIKEAILSYMISRVFTKDQILELYLNQTFFGRGAYGV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
AVAAQNYFNKSVEELTIAESAFIAALPKAPSELNPERNYARVKARRDYVITRMFEDGYIT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCEEEEEEECCCCEE
RDAAKEAMDSPIVLRKRAKEETVTADYYAAQVREEVIRMLNSKEVFYTGGLTIITSLDAK
HHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCHH
MQQLAENSLRKGLREFDRRCGFRKPIANISLDNWQGELKKLPTPPSLLEYKLAVVLDVAD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHEEEEEEEEECC
NHVEIGLIDGSKSKMPIAEMKWARSNFKSVKTLLKKGDVIVVEAIKEGYALRQIPEVNGA
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCHHHCCCCCCCE
IMVMNPNTGQVLASVGGYDFSTSKFDRVTQALRQPGSLSKTFVYLAALENGVKPNQIFND
EEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCC
GPIEISQGPGMPSWRPKNYEGKFLGEITMRTGLEKSRNLITVRVATAVGLTKIVDIIKRF
CCEEECCCCCCCCCCCCCCCCCEEEHHHHHHCCHHHCCEEEEEEHHHHHHHHHHHHHHHC
GINNEPKKVYSMVLGSIETTLSRMTNAYAIIANGGKKVEPHFVELIKDRNGKIIYRRDDR
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCC
ECLACNVSDSNLDTAILEIPKEYIYRVTDEASDYQITSFLTGAIDRGTGYAAKKLGKIIG
CEEEEECCCCCCHHHHHHCCHHHHHHHCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHC
GKTGTSNDSKDTWFVGFTPKIVVGSYVGYDTPKELGKRATGSNVVLPIFIDFMSNAYKDK
CCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHCCCCCCCCEEHHHHHHHHHHHCCCC
PSLPFKVPDSIKLIAVDSATGKITPGGTVIEAFKVNNVQMLENEDMIDNQDNNDIFDYVP
CCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCCHHCCCC
SKEDQSQEIY
CCCCCCCCCH
>Mature Secondary Structure
MYKSLLFCLKIFVFLILVGCGITAYIIYHYSRDLPDYSQLARYYPPSVTRIYSRDGKLME
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHCCCCHHHH
EYAFERRVFVPINSVPSSLIESFIAAEDKNFYNHPGVDLFGIVRAAFLNISNYLHHRRME
HHHHCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GASTITQQVVKNFLLTNEVSLERKIKEAILSYMISRVFTKDQILELYLNQTFFGRGAYGV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
AVAAQNYFNKSVEELTIAESAFIAALPKAPSELNPERNYARVKARRDYVITRMFEDGYIT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCEEEEEEECCCCEE
RDAAKEAMDSPIVLRKRAKEETVTADYYAAQVREEVIRMLNSKEVFYTGGLTIITSLDAK
HHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCHH
MQQLAENSLRKGLREFDRRCGFRKPIANISLDNWQGELKKLPTPPSLLEYKLAVVLDVAD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHEEEEEEEEECC
NHVEIGLIDGSKSKMPIAEMKWARSNFKSVKTLLKKGDVIVVEAIKEGYALRQIPEVNGA
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCHHHCCCCCCCE
IMVMNPNTGQVLASVGGYDFSTSKFDRVTQALRQPGSLSKTFVYLAALENGVKPNQIFND
EEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCC
GPIEISQGPGMPSWRPKNYEGKFLGEITMRTGLEKSRNLITVRVATAVGLTKIVDIIKRF
CCEEECCCCCCCCCCCCCCCCCEEEHHHHHHCCHHHCCEEEEEEHHHHHHHHHHHHHHHC
GINNEPKKVYSMVLGSIETTLSRMTNAYAIIANGGKKVEPHFVELIKDRNGKIIYRRDDR
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCC
ECLACNVSDSNLDTAILEIPKEYIYRVTDEASDYQITSFLTGAIDRGTGYAAKKLGKIIG
CEEEEECCCCCCHHHHHHCCHHHHHHHCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHC
GKTGTSNDSKDTWFVGFTPKIVVGSYVGYDTPKELGKRATGSNVVLPIFIDFMSNAYKDK
CCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHCCCCCCCCEEHHHHHHHHHHHCCCC
PSLPFKVPDSIKLIAVDSATGKITPGGTVIEAFKVNNVQMLENEDMIDNQDNNDIFDYVP
CCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCCHHCCCC
SKEDQSQEIY
CCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11557893