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The map label for this gene is miaB [H]

Identifier: 15893169

GI number: 15893169

Start: 1153076

End: 1154413

Strand: Reverse

Name: miaB [H]

Synonym: RC1246

Alternate gene names: 15893169

Gene position: 1154413-1153076 (Counterclockwise)

Preceding gene: 15893170

Following gene: 15893168

Centisome position: 90.99

GC content: 36.02

Gene sequence:

>1338_bases
ATGAGTAAGAAGCTTTATATTAAAACCTACGGATGTCAGATGAATGTTTATGATTCTGTTAAGATGCAGGATTTGCTTTA
TCCCTTTGGCTATGAACCTACTGAAAATATTGAAGAAGCAGATGTTATTATTCTAAATACTTGTCATATCAGAGAGAAAG
CAGCTGAGAAAACTTATTCTGAACTCGGTCGTATTAAGAAACTACAAGATACAAGAACAAAACAAGGTTTAAGTTCTGCA
ATAATAGTTGTAGCCGGTTGTGTTGCACAGGCGGAAGGGGAGGAGATTTTTACAAGAACTCCTTATGTTGATATTGTTGT
CGGTCCGCAATCTTATTATAACCTACCGGAATTAATCTCAAAAGTCGTTAGACACGAAAAGCACTTAATCGATCTTGATT
TTGTTGAAGAAGCAAAATTTGATCAGCTACCTGAGCAATTATATCCGCAAGGAACAAGTGCCTTTATATCGGTACAGGAA
GGGTGCGATAAATTTTGTACTTTCTGCGTAGTACCTTATACTAGAGGTGCTGAATTTTCAAGAAATGTAGAGCAGGTTTA
CAGAGAAGCACTAAAAGTAGTTAGTAGCGGTGCTAAAGAAATTATGTTACTTGGACAAAATGTCAATGCATATCACGGTA
AAGGTCCTGCAGATAAAATATTTAGTCTAGCCGATTTACTCAAACATCTAGCACAAATTCCCAATTTAGAGAGATTACGT
TATACCACCTCACACCCGATAGATATGAATAATGATCTAATAAAGCTGTACGGCACCGAACCTAAATTAATGCCGTTTCT
ACATCTACCGGTGCAGTCAGGTTCAAATAAAATATTAAAAGCCATGAACCGCAAACATGATCGGGAATATTATTTTGATA
TAATTAATCGCTTAAGAGAAGCAAGGCCTGATATAGTTTTATCCTCTGATTTTATTGTCGGGTTTCCCGGTGAAACAGAT
GAAGATTTTGAGGATACATTAGATTTGGTCCGTAGAGTAAAATACGGTCAGTGTTATTCGTTTAAATATAGCCCACGTCC
CGGCACACCTGGTGCAACTAGAACCGACCAAATACCTGAACATATAAAATCAGAGAGATTAACTATATTACAACAAGAAC
TCATGGCTCAGCAACTAGCTTTTAATACAAGTTGTGTTGGTAGTACTATGAAAGTTCTATTTGATCGCAATGGTAAATTC
GACGACCAGATAATAGGTAAAACTCCATACATGCAGTCCGTATATATCCAAAACCCTAATAAATCTTTACTTGGTAAAAT
CAGTGATGTAAAAATTACTAAGGCTTCACTTAATAGTTTAACCGGCGAAATATTATAA

Upstream 100 bases:

>100_bases
CGTTATACGCAGCTCATCTTGCTTTGTGAACTCCTCGCTCTTTTTGAATTTGATCTTCGTATACTAGGCTTTTAGTTTAA
AGATATATATATATAAAATT

Downstream 100 bases:

>100_bases
GTCATGTATAAATCTCTTCTTTTTTGTTTAAAAATTTTTGTCTTCCTTATTTTAGTAGGTTGCGGGATTACTGCTTATAT
AATATACCACTATTCACGTG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSKKLYIKTYGCQMNVYDSVKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYSELGRIKKLQDTRTKQGLSSA
IIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELISKVVRHEKHLIDLDFVEEAKFDQLPEQLYPQGTSAFISVQE
GCDKFCTFCVVPYTRGAEFSRNVEQVYREALKVVSSGAKEIMLLGQNVNAYHGKGPADKIFSLADLLKHLAQIPNLERLR
YTTSHPIDMNNDLIKLYGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLREARPDIVLSSDFIVGFPGETD
EDFEDTLDLVRRVKYGQCYSFKYSPRPGTPGATRTDQIPEHIKSERLTILQQELMAQQLAFNTSCVGSTMKVLFDRNGKF
DDQIIGKTPYMQSVYIQNPNKSLLGKISDVKITKASLNSLTGEIL

Sequences:

>Translated_445_residues
MSKKLYIKTYGCQMNVYDSVKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYSELGRIKKLQDTRTKQGLSSA
IIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELISKVVRHEKHLIDLDFVEEAKFDQLPEQLYPQGTSAFISVQE
GCDKFCTFCVVPYTRGAEFSRNVEQVYREALKVVSSGAKEIMLLGQNVNAYHGKGPADKIFSLADLLKHLAQIPNLERLR
YTTSHPIDMNNDLIKLYGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLREARPDIVLSSDFIVGFPGETD
EDFEDTLDLVRRVKYGQCYSFKYSPRPGTPGATRTDQIPEHIKSERLTILQQELMAQQLAFNTSCVGSTMKVLFDRNGKF
DDQIIGKTPYMQSVYIQNPNKSLLGKISDVKITKASLNSLTGEIL
>Mature_444_residues
SKKLYIKTYGCQMNVYDSVKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYSELGRIKKLQDTRTKQGLSSAI
IVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELISKVVRHEKHLIDLDFVEEAKFDQLPEQLYPQGTSAFISVQEG
CDKFCTFCVVPYTRGAEFSRNVEQVYREALKVVSSGAKEIMLLGQNVNAYHGKGPADKIFSLADLLKHLAQIPNLERLRY
TTSHPIDMNNDLIKLYGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLREARPDIVLSSDFIVGFPGETDE
DFEDTLDLVRRVKYGQCYSFKYSPRPGTPGATRTDQIPEHIKSERLTILQQELMAQQLAFNTSCVGSTMKVLFDRNGKFD
DQIIGKTPYMQSVYIQNPNKSLLGKISDVKITKASLNSLTGEIL

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=34.8008385744235, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=34.8008385744235, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI93277076, Length=451, Percent_Identity=27.4944567627494, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=47.6510067114094, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1787057, Length=455, Percent_Identity=29.8901098901099, Blast_Score=191, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17553146, Length=421, Percent_Identity=33.729216152019, Blast_Score=249, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI71996771, Length=315, Percent_Identity=27.6190476190476, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356207, Length=428, Percent_Identity=32.9439252336449, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=28.8546255506608, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50533; Mature: 50401

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLYIKTYGCQMNVYDSVKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYS
CCCEEEEEEECCEEECCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHH
ELGRIKKLQDTRTKQGLSSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCHHHCCHHHHHH
KVVRHEKHLIDLDFVEEAKFDQLPEQLYPQGTSAFISVQEGCDKFCTFCVVPYTRGAEFS
HHHHHHHHEECCHHHHHHHHHHCCHHHCCCCCHHEEEHHHHHHHHHHEEECCCCCCCHHH
RNVEQVYREALKVVSSGAKEIMLLGQNVNAYHGKGPADKIFSLADLLKHLAQIPNLERLR
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHEE
YTTSHPIDMNNDLIKLYGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLRE
ECCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
ARPDIVLSSDFIVGFPGETDEDFEDTLDLVRRVKYGQCYSFKYSPRPGTPGATRTDQIPE
CCCCEEEECCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
HIKSERLTILQQELMAQQLAFNTSCVGSTMKVLFDRNGKFDDQIIGKTPYMQSVYIQNPN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCEEEEEEECCC
KSLLGKISDVKITKASLNSLTGEIL
HHHHCCCCCCEEEHHHHHHHHCCCC
>Mature Secondary Structure 
SKKLYIKTYGCQMNVYDSVKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYS
CCEEEEEEECCEEECCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHH
ELGRIKKLQDTRTKQGLSSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCHHHCCHHHHHH
KVVRHEKHLIDLDFVEEAKFDQLPEQLYPQGTSAFISVQEGCDKFCTFCVVPYTRGAEFS
HHHHHHHHEECCHHHHHHHHHHCCHHHCCCCCHHEEEHHHHHHHHHHEEECCCCCCCHHH
RNVEQVYREALKVVSSGAKEIMLLGQNVNAYHGKGPADKIFSLADLLKHLAQIPNLERLR
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHEE
YTTSHPIDMNNDLIKLYGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLRE
ECCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
ARPDIVLSSDFIVGFPGETDEDFEDTLDLVRRVKYGQCYSFKYSPRPGTPGATRTDQIPE
CCCCEEEECCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
HIKSERLTILQQELMAQQLAFNTSCVGSTMKVLFDRNGKFDDQIIGKTPYMQSVYIQNPN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCEEEEEEECCC
KSLLGKISDVKITKASLNSLTGEIL
HHHHCCCCCCEEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA