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The map label for this gene is ligA [H]

Identifier: 15893019

GI number: 15893019

Start: 1021136

End: 1023205

Strand: Reverse

Name: ligA [H]

Synonym: RC1096

Alternate gene names: 15893019

Gene position: 1023205-1021136 (Counterclockwise)

Preceding gene: 15893020

Following gene: 15893018

Centisome position: 80.65

GC content: 33.53

Gene sequence:

>2070_bases
ATGCAAAATATTGATTTAATATCTGAAGAGGAAGCACAAAAATTACTAGAAGAACTCGCTGATAAAATTGCAGCGTATAA
CCATGCTTATTATATAGAAGATAATCCTTTAGTTTCTGATAGCGAGTATGATCAGCTATTTAATACCAATCTCAAGTTAG
AACAAAAATTTCCTCATTTAATTTTAGAAAATAGTCCTAGCAAAAAAGTAGGGGCTAAAATAGCAAATAAATTTGCTAAA
GTTACGCATCAAGTGCCCATGCTATCTCTCAGTAATGCTTTTGATGAGCAAGATGTGAGAGATTTTGTAGACCGTATAAA
AATTTTCTTACGCCTTAATGAGTTTGCTCCTATCTTTTGTGAACCTAAAATAGACGGTTTGTCTTTTTCTGCTGTTTATA
AACACGGGGTGCTTACAACGGGAGCTACAAGAGGTGACGGATATGTCGGTGAAGATATAACAGCAAATATTAAAACAATC
AAAAATTTTCCTCATAAAATAGATAATGTTCCAGAGTTTTTAGAAGTACGAGGGGAAATTTATATTGAAAAACAAGATTT
TTTAAATCTAAATAAAGAACAAGAAGAGCAAGGCAAGGATAAATTTGCCAATCCTCGTAATGCTGCTGCCGGATCACTTC
GTCAGCTTGATTCTTCTATTACGGCTAAAAGACCGCTCAAATATTTTGTATATTCAGGAGGGGTAACTGAACAGAATCTC
GCTTCTTCACAAGATCAATTACTTACAAAATTAAAAGAATGCGGCTTTAACATTAATGAAATATCTAAACTTGCGAGTTC
TGAAGAAGAAATTTTCGCCTTTTACGAATATCTCAAAACAAATAGAGAAAATTTACCTTATGAAATTGATGGAGTAGTAT
ATAAGCTAAATGATTTTGCACTGCAAAATAGAATGGGCTTTATTGCACGAAGTCCACGCTTTGCTACCGCTCATAAATTT
CCTGCAATAATAGGACAAACAAAGTTACTTTCTATTACTGTACAGGTCGGTAGAACTGGTACGTTAACGCCGGTAGCCGA
ACTTGAACCCATAGAAATAGGCGGGGTAACTGTTAGCAGAGCAACATTACATAATTTCCAAGAAATTGCACGAAAAGATT
TGCGGATTAAGGATTATGTATTCCTGCAACGTGCTGGTGACGTTATTCCTAAAATCATGGGAGTTGATTTTGATAAACGT
CCTAATGATACAGAAACGTTTGATACTCCTTTATTTTGTCTGTCATGCAATTCTAAATTGCATTATACGCCTGAAGATAT
TATTATACGCTGTGATAATGGTCTTAACTGCCCTGCTCAGAATTATGAGCGTATCCGTCATTTTGTATCGAAAAATGCTA
TGGATATTGAGGGGCTTGGGCGTAAGCAAGTTGAGTTTTTAATAGATAAGGGATTGATTAGTAATCTTTTAGATATTTTC
TTTTTAAAAGAAAAAAATGACTCTAGCTTAGCAAAACTTGAAAATATGGATGGATGGGGTAAAAAATCAGTAGAAAATCT
TTTTAAAAATATAGAAAAATCAAAAAACGTTAGTTTGCCAAGATTTATATATGCATTAGGCATAAGGCATATCGGTGAAC
AAAATGCTAAATTACTTGCTAGAGAATTTGGCAGCTATAATAATTTTATAGCTCAAATGGAATTGCTTAGAACAAATGAG
CCTGACATTTATCAAAAACTAAAGAATTTAGAAGGAATCGGTGATAAGATTTTAGTAGATATTATTGATTTTTTTGATGT
TAAAGAAAATATTGAGCTTATCAAAAAACTTGGTGAAATATTAAATATTGAAGATTATAAAGAGACTAGAGAACAGAGTG
GTTTAACGGACAAGATAGTCGTATTTACCGGTAGTTTACCGACGATATCTAGAGCAGAAGCTAAAGCAACGGCTGAGAAG
CTTGGGGCTAAAGTTGCAGTTGGCGTATCATCGAACACGGATTTAGTTGTAGCCGGCGTTGATGCAGGCAGTAAACTTAA
AAAAGCTAAAGAGCTTAATATTAAAATTATTGATGAAGAAGAATGGCTTACGCTTATAAAAAATGTTTAA

Upstream 100 bases:

>100_bases
AATGCTGAAAGACTAAAACGAGCAATTAGCGATTTTAAAGAAAATAAAAATTTTAGGAATTGGATTTGATTGTAAATTAA
AATAAAAGTATGAACTACTA

Downstream 100 bases:

>100_bases
AGCAAGGCATTGCTAACTAAATGATAGTAAATAAAAAACTCGATGTTATTTTCACGAAAGCGGGAATGACATCGGAAAGC
TAAACTACACTATAAAACAA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 689; Mature: 689

Protein sequence:

>689_residues
MQNIDLISEEEAQKLLEELADKIAAYNHAYYIEDNPLVSDSEYDQLFNTNLKLEQKFPHLILENSPSKKVGAKIANKFAK
VTHQVPMLSLSNAFDEQDVRDFVDRIKIFLRLNEFAPIFCEPKIDGLSFSAVYKHGVLTTGATRGDGYVGEDITANIKTI
KNFPHKIDNVPEFLEVRGEIYIEKQDFLNLNKEQEEQGKDKFANPRNAAAGSLRQLDSSITAKRPLKYFVYSGGVTEQNL
ASSQDQLLTKLKECGFNINEISKLASSEEEIFAFYEYLKTNRENLPYEIDGVVYKLNDFALQNRMGFIARSPRFATAHKF
PAIIGQTKLLSITVQVGRTGTLTPVAELEPIEIGGVTVSRATLHNFQEIARKDLRIKDYVFLQRAGDVIPKIMGVDFDKR
PNDTETFDTPLFCLSCNSKLHYTPEDIIIRCDNGLNCPAQNYERIRHFVSKNAMDIEGLGRKQVEFLIDKGLISNLLDIF
FLKEKNDSSLAKLENMDGWGKKSVENLFKNIEKSKNVSLPRFIYALGIRHIGEQNAKLLAREFGSYNNFIAQMELLRTNE
PDIYQKLKNLEGIGDKILVDIIDFFDVKENIELIKKLGEILNIEDYKETREQSGLTDKIVVFTGSLPTISRAEAKATAEK
LGAKVAVGVSSNTDLVVAGVDAGSKLKKAKELNIKIIDEEEWLTLIKNV

Sequences:

>Translated_689_residues
MQNIDLISEEEAQKLLEELADKIAAYNHAYYIEDNPLVSDSEYDQLFNTNLKLEQKFPHLILENSPSKKVGAKIANKFAK
VTHQVPMLSLSNAFDEQDVRDFVDRIKIFLRLNEFAPIFCEPKIDGLSFSAVYKHGVLTTGATRGDGYVGEDITANIKTI
KNFPHKIDNVPEFLEVRGEIYIEKQDFLNLNKEQEEQGKDKFANPRNAAAGSLRQLDSSITAKRPLKYFVYSGGVTEQNL
ASSQDQLLTKLKECGFNINEISKLASSEEEIFAFYEYLKTNRENLPYEIDGVVYKLNDFALQNRMGFIARSPRFATAHKF
PAIIGQTKLLSITVQVGRTGTLTPVAELEPIEIGGVTVSRATLHNFQEIARKDLRIKDYVFLQRAGDVIPKIMGVDFDKR
PNDTETFDTPLFCLSCNSKLHYTPEDIIIRCDNGLNCPAQNYERIRHFVSKNAMDIEGLGRKQVEFLIDKGLISNLLDIF
FLKEKNDSSLAKLENMDGWGKKSVENLFKNIEKSKNVSLPRFIYALGIRHIGEQNAKLLAREFGSYNNFIAQMELLRTNE
PDIYQKLKNLEGIGDKILVDIIDFFDVKENIELIKKLGEILNIEDYKETREQSGLTDKIVVFTGSLPTISRAEAKATAEK
LGAKVAVGVSSNTDLVVAGVDAGSKLKKAKELNIKIIDEEEWLTLIKNV
>Mature_689_residues
MQNIDLISEEEAQKLLEELADKIAAYNHAYYIEDNPLVSDSEYDQLFNTNLKLEQKFPHLILENSPSKKVGAKIANKFAK
VTHQVPMLSLSNAFDEQDVRDFVDRIKIFLRLNEFAPIFCEPKIDGLSFSAVYKHGVLTTGATRGDGYVGEDITANIKTI
KNFPHKIDNVPEFLEVRGEIYIEKQDFLNLNKEQEEQGKDKFANPRNAAAGSLRQLDSSITAKRPLKYFVYSGGVTEQNL
ASSQDQLLTKLKECGFNINEISKLASSEEEIFAFYEYLKTNRENLPYEIDGVVYKLNDFALQNRMGFIARSPRFATAHKF
PAIIGQTKLLSITVQVGRTGTLTPVAELEPIEIGGVTVSRATLHNFQEIARKDLRIKDYVFLQRAGDVIPKIMGVDFDKR
PNDTETFDTPLFCLSCNSKLHYTPEDIIIRCDNGLNCPAQNYERIRHFVSKNAMDIEGLGRKQVEFLIDKGLISNLLDIF
FLKEKNDSSLAKLENMDGWGKKSVENLFKNIEKSKNVSLPRFIYALGIRHIGEQNAKLLAREFGSYNNFIAQMELLRTNE
PDIYQKLKNLEGIGDKILVDIIDFFDVKENIELIKKLGEILNIEDYKETREQSGLTDKIVVFTGSLPTISRAEAKATAEK
LGAKVAVGVSSNTDLVVAGVDAGSKLKKAKELNIKIIDEEEWLTLIKNV

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=689, Percent_Identity=41.799709724238, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI87082305, Length=600, Percent_Identity=22.6666666666667, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 77839; Mature: 77839

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNIDLISEEEAQKLLEELADKIAAYNHAYYIEDNPLVSDSEYDQLFNTNLKLEQKFPHL
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHCCCCEEHHCCCCE
ILENSPSKKVGAKIANKFAKVTHQVPMLSLSNAFDEQDVRDFVDRIKIFLRLNEFAPIFC
EECCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEE
EPKIDGLSFSAVYKHGVLTTGATRGDGYVGEDITANIKTIKNFPHKIDNVPEFLEVRGEI
CCCCCCCCHHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHCCCHHHCCCHHHHHCCCCE
YIEKQDFLNLNKEQEEQGKDKFANPRNAAAGSLRQLDSSITAKRPLKYFVYSGGVTEQNL
EEEHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHH
ASSQDQLLTKLKECGFNINEISKLASSEEEIFAFYEYLKTNRENLPYEIDGVVYKLNDFA
HCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEECCEEEEEHHHH
LQNRMGFIARSPRFATAHKFPAIIGQTKLLSITVQVGRTGTLTPVAELEPIEIGGVTVSR
HHHCCCHHCCCCCCCHHHHCHHHHCCCEEEEEEEEECCCCCCCCCCCCCCEEECCEEEHH
ATLHNFQEIARKDLRIKDYVFLQRAGDVIPKIMGVDFDKRPNDTETFDTPLFCLSCNSKL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCE
HYTPEDIIIRCDNGLNCPAQNYERIRHFVSKNAMDIEGLGRKQVEFLIDKGLISNLLDIF
EECCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FLKEKNDSSLAKLENMDGWGKKSVENLFKNIEKSKNVSLPRFIYALGIRHIGEQNAKLLA
EEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHH
REFGSYNNFIAQMELLRTNEPDIYQKLKNLEGIGDKILVDIIDFFDVKENIELIKKLGEI
HHHCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LNIEDYKETREQSGLTDKIVVFTGSLPTISRAEAKATAEKLGAKVAVGVSSNTDLVVAGV
CCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEC
DAGSKLKKAKELNIKIIDEEEWLTLIKNV
CCCHHHHHHHHCCEEEECCHHHHHHHHCC
>Mature Secondary Structure
MQNIDLISEEEAQKLLEELADKIAAYNHAYYIEDNPLVSDSEYDQLFNTNLKLEQKFPHL
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHCCCCEEHHCCCCE
ILENSPSKKVGAKIANKFAKVTHQVPMLSLSNAFDEQDVRDFVDRIKIFLRLNEFAPIFC
EECCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEE
EPKIDGLSFSAVYKHGVLTTGATRGDGYVGEDITANIKTIKNFPHKIDNVPEFLEVRGEI
CCCCCCCCHHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHCCCHHHCCCHHHHHCCCCE
YIEKQDFLNLNKEQEEQGKDKFANPRNAAAGSLRQLDSSITAKRPLKYFVYSGGVTEQNL
EEEHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHH
ASSQDQLLTKLKECGFNINEISKLASSEEEIFAFYEYLKTNRENLPYEIDGVVYKLNDFA
HCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEECCEEEEEHHHH
LQNRMGFIARSPRFATAHKFPAIIGQTKLLSITVQVGRTGTLTPVAELEPIEIGGVTVSR
HHHCCCHHCCCCCCCHHHHCHHHHCCCEEEEEEEEECCCCCCCCCCCCCCEEECCEEEHH
ATLHNFQEIARKDLRIKDYVFLQRAGDVIPKIMGVDFDKRPNDTETFDTPLFCLSCNSKL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCE
HYTPEDIIIRCDNGLNCPAQNYERIRHFVSKNAMDIEGLGRKQVEFLIDKGLISNLLDIF
EECCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FLKEKNDSSLAKLENMDGWGKKSVENLFKNIEKSKNVSLPRFIYALGIRHIGEQNAKLLA
EEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHH
REFGSYNNFIAQMELLRTNEPDIYQKLKNLEGIGDKILVDIIDFFDVKENIELIKKLGEI
HHHCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LNIEDYKETREQSGLTDKIVVFTGSLPTISRAEAKATAEKLGAKVAVGVSSNTDLVVAGV
CCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEC
DAGSKLKKAKELNIKIIDEEEWLTLIKNV
CCCHHHHHHHHCCEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA