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The map label for this gene is tgt

Identifier: 15893020

GI number: 15893020

Start: 1023491

End: 1024576

Strand: Reverse

Name: tgt

Synonym: RC1097

Alternate gene names: 15893020

Gene position: 1024576-1023491 (Counterclockwise)

Preceding gene: 15893023

Following gene: 15893019

Centisome position: 80.75

GC content: 40.98

Gene sequence:

>1086_bases
GTGTCAAAATTTTCTTTTAATATTCATCATCAGCATAAAAAAGCTAGAAGCGGTATTATCGTAACCGCACACGGTGAGAT
GCGTACACCCGCTTTTATGCCAGTTGGAACTAGAGGAACGGTTAAGGCAATGCTACCTGAATCGGTAGCAGAAACGGGGG
CGGATATATTGCTTGGCAATACTTACCACTTAATGCTTCAGCCGACTGCCGAACGTATAGTACAGCTTGGTGGCTTACAT
AAATTCATGAATTGGGATAAGCCGATATTAACCGATTCTGGCGGTTTTCAGGTAATGTCGCTATCGAAGTTATGCAAGAT
AACCGAAGAGGGAGTAAGTTTCAGCTCTCATATTAACGGCGATAAATATATGTTAACTCCTGAGCGTTCTACCGAAATAC
AATATCTGCTCGGTAGCACAATCACTATGGCTTTTGATGAATGTACGCCTTACCCTGCAACCTTTGAAGAAGCTAAAACT
TCTATGCAGCTTACGACTAGATGGGCGAATAGATCACGTAATGCTTTTGTTAAGCGGGAAGGCTATGCACAATTCGGTAT
TATTCAAGGTAGCGTTTACGAAGAATTACGTGAGCAATCGGCTAAGGATCTAGTAGAACTTGATTTTGAGGGTTATGCTA
TAGGTGGACTTGCAGTAGGTGAGGGGCAGGAGCTTATGTTTAAAGTACTTGATTATGCTCCTGAGTTTCTGCCGCAAAAT
AAGCCGCGTTATTTAATGGGAGTCGGTAAGCCTGTTGATATTATAGGTGCGGTTAGCAGGGGTATAGATATGTTTGACTG
CGTAATCCCGACACGCTCAGGTCGCAACGGTCAGGCTTTTACGAAATACGGTACGGTAAATATCCGCAATAGCAAATATG
CCGATGATAACAAGCCGCTAGAACATGATTGCCTATGTCCTGCTTGTAGAAACTATAGTAAAGCTTATCTACATCATTTA
GTTAGAATCGGTGAAATACTTGGCTCTATGCTAATGACATGGCATAATTTAACATATTTCCAAAATCTCATGAGCCGTAT
TAGAGCATATATTAAACTAGGTAAAGATTTTGATTTTGATTCTTAA

Upstream 100 bases:

>100_bases
TAAATTACAGCATAAAACAACTGCTTTTAATCTTAATTTTTATATTCCAGGATAAACTATAGTATGCTAAGATATTGCTT
TTAAAAATAAGTGATTATCA

Downstream 100 bases:

>100_bases
AGTGACTAGTTATAAAACATGCCTTATTACATGTAAACGTACTTAAGTGGGTTATGTTATGAAAAAAGTTTGTGCAGATC
ACATTTCAGTGATTGTAACT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSKFSFNIHHQHKKARSGIIVTAHGEMRTPAFMPVGTRGTVKAMLPESVAETGADILLGNTYHLMLQPTAERIVQLGGLH
KFMNWDKPILTDSGGFQVMSLSKLCKITEEGVSFSSHINGDKYMLTPERSTEIQYLLGSTITMAFDECTPYPATFEEAKT
SMQLTTRWANRSRNAFVKREGYAQFGIIQGSVYEELREQSAKDLVELDFEGYAIGGLAVGEGQELMFKVLDYAPEFLPQN
KPRYLMGVGKPVDIIGAVSRGIDMFDCVIPTRSGRNGQAFTKYGTVNIRNSKYADDNKPLEHDCLCPACRNYSKAYLHHL
VRIGEILGSMLMTWHNLTYFQNLMSRIRAYIKLGKDFDFDS

Sequences:

>Translated_361_residues
MSKFSFNIHHQHKKARSGIIVTAHGEMRTPAFMPVGTRGTVKAMLPESVAETGADILLGNTYHLMLQPTAERIVQLGGLH
KFMNWDKPILTDSGGFQVMSLSKLCKITEEGVSFSSHINGDKYMLTPERSTEIQYLLGSTITMAFDECTPYPATFEEAKT
SMQLTTRWANRSRNAFVKREGYAQFGIIQGSVYEELREQSAKDLVELDFEGYAIGGLAVGEGQELMFKVLDYAPEFLPQN
KPRYLMGVGKPVDIIGAVSRGIDMFDCVIPTRSGRNGQAFTKYGTVNIRNSKYADDNKPLEHDCLCPACRNYSKAYLHHL
VRIGEILGSMLMTWHNLTYFQNLMSRIRAYIKLGKDFDFDS
>Mature_360_residues
SKFSFNIHHQHKKARSGIIVTAHGEMRTPAFMPVGTRGTVKAMLPESVAETGADILLGNTYHLMLQPTAERIVQLGGLHK
FMNWDKPILTDSGGFQVMSLSKLCKITEEGVSFSSHINGDKYMLTPERSTEIQYLLGSTITMAFDECTPYPATFEEAKTS
MQLTTRWANRSRNAFVKREGYAQFGIIQGSVYEELREQSAKDLVELDFEGYAIGGLAVGEGQELMFKVLDYAPEFLPQNK
PRYLMGVGKPVDIIGAVSRGIDMFDCVIPTRSGRNGQAFTKYGTVNIRNSKYADDNKPLEHDCLCPACRNYSKAYLHHLV
RIGEILGSMLMTWHNLTYFQNLMSRIRAYIKLGKDFDFDS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=347, Percent_Identity=40.0576368876081, Blast_Score=278, Evalue=7e-75,
Organism=Escherichia coli, GI1786607, Length=352, Percent_Identity=56.5340909090909, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17544680, Length=339, Percent_Identity=41.8879056047198, Blast_Score=269, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI25146502, Length=170, Percent_Identity=31.1764705882353, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19920510, Length=339, Percent_Identity=43.0678466076696, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI21355311, Length=181, Percent_Identity=26.5193370165746, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_RICAE (C3PLJ4)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845577.1
- ProteinModelPortal:   C3PLJ4
- SMR:   C3PLJ4
- GeneID:   7815981
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF1005
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 40590; Mature: 40459

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: ACT_SITE 92-92 BINDING 93-93

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFSFNIHHQHKKARSGIIVTAHGEMRTPAFMPVGTRGTVKAMLPESVAETGADILLGN
CCCEEEEHHHHHHHHHCCEEEEECCCCCCCCEEECCCCCCCHHHCHHHHHHCCCCEEECC
TYHLMLQPTAERIVQLGGLHKFMNWDKPILTDSGGFQVMSLSKLCKITEEGVSFSSHING
EEEEEECCHHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHCCCHHHCCCC
DKYMLTPERSTEIQYLLGSTITMAFDECTPYPATFEEAKTSMQLTTRWANRSRNAFVKRE
CEEEECCCCCCCHHHHHCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECC
GYAQFGIIQGSVYEELREQSAKDLVELDFEGYAIGGLAVGEGQELMFKVLDYAPEFLPQN
CCEEEEEEECHHHHHHHHHHHHHHEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCC
KPRYLMGVGKPVDIIGAVSRGIDMFDCVIPTRSGRNGQAFTKYGTVNIRNSKYADDNKPL
CCCEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCC
EHDCLCPACRNYSKAYLHHLVRIGEILGSMLMTWHNLTYFQNLMSRIRAYIKLGKDFDFD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
S
C
>Mature Secondary Structure 
SKFSFNIHHQHKKARSGIIVTAHGEMRTPAFMPVGTRGTVKAMLPESVAETGADILLGN
CCEEEEHHHHHHHHHCCEEEEECCCCCCCCEEECCCCCCCHHHCHHHHHHCCCCEEECC
TYHLMLQPTAERIVQLGGLHKFMNWDKPILTDSGGFQVMSLSKLCKITEEGVSFSSHING
EEEEEECCHHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHCCCHHHCCCC
DKYMLTPERSTEIQYLLGSTITMAFDECTPYPATFEEAKTSMQLTTRWANRSRNAFVKRE
CEEEECCCCCCCHHHHHCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECC
GYAQFGIIQGSVYEELREQSAKDLVELDFEGYAIGGLAVGEGQELMFKVLDYAPEFLPQN
CCEEEEEEECHHHHHHHHHHHHHHEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCC
KPRYLMGVGKPVDIIGAVSRGIDMFDCVIPTRSGRNGQAFTKYGTVNIRNSKYADDNKPL
CCCEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCC
EHDCLCPACRNYSKAYLHHLVRIGEILGSMLMTWHNLTYFQNLMSRIRAYIKLGKDFDFD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA