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The map label for this gene is dat [H]

Identifier: 15892793

GI number: 15892793

Start: 818046

End: 818552

Strand: Reverse

Name: dat [H]

Synonym: RC0870

Alternate gene names: 15892793

Gene position: 818552-818046 (Counterclockwise)

Preceding gene: 15892794

Following gene: 15892792

Centisome position: 64.52

GC content: 34.32

Gene sequence:

>507_bases
ATGAGTTTAAAATCCGCTTGGCTAGATACGCCTCTTGGTTCTATGCTGGCAATTAGTGATACAGAGAGGCTTTATTTGCT
CGATTTTGTTGAAAGTAAAGGATTAGAGTATAAAATTAAAAGACTTAAGATTAACACAAAATCGGCTATTACTGAAGATC
GCACTAGACCGATTATATCAATTGAAGAGGAGCTTAAATCTTACTTTAGCGGTACTTTGCAAAAATTTAATACTCCGATA
TATCTTTTAGGAAGCAAATTTCAAAAATTAGTATGGCAAGAGCTAATGAATGTACCTTATGGTGAAACAAGAAGTTACTT
TACCCAAGCTAAATTAATAGGTATGGAAAGATCTTATAGAGCCGTTGCAAATGCTAATGGAGCAAATAAATTTGTTATAA
TTATTCCGTGTCACTGCATCATCAATAATAACGGTAAGCTTGGCGGTTATGGTTCAGGGCTAGATCACAAAAAATGGCTT
ATCGAGCATGAGAGACAGAAGTATTAA

Upstream 100 bases:

>100_bases
ATCATAGTGATTACCTATAAATTGTTAGTTTTAAAAATATAAAATTGTTAAATAAAAAATCAATATTATAATATTAAGAA
ATCATATTTAGGGTAAAATA

Downstream 100 bases:

>100_bases
ATAAAAAATCAATGCCTGAATTTCGGTTACAGGAGTTTTAAAAAGTTCATATAATATAATTATTATATTATAGGATAAAT
TAATATGTTTAATAAGCTTC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MSLKSAWLDTPLGSMLAISDTERLYLLDFVESKGLEYKIKRLKINTKSAITEDRTRPIISIEEELKSYFSGTLQKFNTPI
YLLGSKFQKLVWQELMNVPYGETRSYFTQAKLIGMERSYRAVANANGANKFVIIIPCHCIINNNGKLGGYGSGLDHKKWL
IEHERQKY

Sequences:

>Translated_168_residues
MSLKSAWLDTPLGSMLAISDTERLYLLDFVESKGLEYKIKRLKINTKSAITEDRTRPIISIEEELKSYFSGTLQKFNTPI
YLLGSKFQKLVWQELMNVPYGETRSYFTQAKLIGMERSYRAVANANGANKFVIIIPCHCIINNNGKLGGYGSGLDHKKWL
IEHERQKY
>Mature_167_residues
SLKSAWLDTPLGSMLAISDTERLYLLDFVESKGLEYKIKRLKINTKSAITEDRTRPIISIEEELKSYFSGTLQKFNTPIY
LLGSKFQKLVWQELMNVPYGETRSYFTQAKLIGMERSYRAVANANGANKFVIIIPCHCIINNNGKLGGYGSGLDHKKWLI
EHERQKY

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=172, Percent_Identity=26.7441860465116, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1787596, Length=161, Percent_Identity=34.1614906832298, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1788542, Length=98, Percent_Identity=42.8571428571429, Blast_Score=84, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=103, Percent_Identity=34.9514563106796, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533070, Length=103, Percent_Identity=34.9514563106796, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320001, Length=86, Percent_Identity=38.3720930232558, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19288; Mature: 19156

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKSAWLDTPLGSMLAISDTERLYLLDFVESKGLEYKIKRLKINTKSAITEDRTRPIIS
CCCCCCCCCCCCCCEEEECCCCEEEEEEEHHCCCCEEEEEEEEECCHHHHCCCCCCCEEE
IEEELKSYFSGTLQKFNTPIYLLGSKFQKLVWQELMNVPYGETRSYFTQAKLIGMERSYR
HHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHH
AVANANGANKFVIIIPCHCIINNNGKLGGYGSGLDHKKWLIEHERQKY
HHHCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SLKSAWLDTPLGSMLAISDTERLYLLDFVESKGLEYKIKRLKINTKSAITEDRTRPIIS
CCCCCCCCCCCCCEEEECCCCEEEEEEEHHCCCCEEEEEEEEECCHHHHCCCCCCCEEE
IEEELKSYFSGTLQKFNTPIYLLGSKFQKLVWQELMNVPYGETRSYFTQAKLIGMERSYR
HHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHH
AVANANGANKFVIIIPCHCIINNNGKLGGYGSGLDHKKWLIEHERQKY
HHHCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA