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The map label for this gene is ada [C]

Identifier: 15892792

GI number: 15892792

Start: 817728

End: 817961

Strand: Reverse

Name: ada [C]

Synonym: RC0869

Alternate gene names: 15892792

Gene position: 817961-817728 (Counterclockwise)

Preceding gene: 15892793

Following gene: 15892791

Centisome position: 64.47

GC content: 32.91

Gene sequence:

>234_bases
ATGTTTAATAAGCTTCAAAGACAAGAATATTATCAGGCATTAATTAATAAAGATGACAGATATGAAAATATCTTTTTTGT
AGCGGTTAAAATAACAAGAGTATTTTGTAGACCGACTTGTCCTGTACGGAAACCTAAATTTGAAAATTGTGAATTTTATA
AAACTGCTAAGGAAGCTTGGCATGCTTCATATAGACCTGGTCAGCGTTGCAAGCTTCTTTCACATCCTTGGTGA

Upstream 100 bases:

>100_bases
GAGACAGAAGTATTAAATAAAAAATCAATGCCTGAATTTCGGTTACAGGAGTTTTAAAAAGTTCATATAATATAATTATT
ATATTATAGGATAAATTAAT

Downstream 100 bases:

>100_bases
AGCTTCAGGTTTAGTTTGTACTCTTGTTAATGCTATTGAGTTTAATCCTGCTAAAAGATGGAAAGATAGTGATTGTGATG
AGTTATCAGTAGATGCTTCT

Product: putative methylated-DNA--protein-cysteine methyltransferase

Products: DNA lacking O-methylguanine; protein S-methyl-L-cysteine [C]

Alternate protein names: AraC Family Transcriptional Regulator; Ada Regulatory Protein; Methylated-DNA/Protein-Cysteine Methyltransferase; Transcriptional Regulator AraC Family; Methylated-DNA-(Protein)-Cysteine S-Methyltransferase; Ada Protein; Regulatory Protein Ada; 3-Methyladenine DNA Glycosylase; Methylated-DNA-[Protein]-Cysteine S-Methyltransferase; Regulatory Protein ADA; Transcriptional Regulator; ADA Regulatory Protein; 6-O-Methylguanine-DNA Methyltransferase; DNA Methyltransferase; Methylphosphotriester-DNA Alkyltransferase; Transcription Regulator Protein; Regulatory Protein Of Adaptative Response; DNA-O6-Methylguanine-Protein-Cysteine S-Methyltransferase; DNA-3-Methyladenine Glycosidase II; DNA Methylation And Regulatory Protein; Adenosine Deaminase

Number of amino acids: Translated: 77; Mature: 77

Protein sequence:

>77_residues
MFNKLQRQEYYQALINKDDRYENIFFVAVKITRVFCRPTCPVRKPKFENCEFYKTAKEAWHASYRPGQRCKLLSHPW

Sequences:

>Translated_77_residues
MFNKLQRQEYYQALINKDDRYENIFFVAVKITRVFCRPTCPVRKPKFENCEFYKTAKEAWHASYRPGQRCKLLSHPW
>Mature_77_residues
MFNKLQRQEYYQALINKDDRYENIFFVAVKITRVFCRPTCPVRKPKFENCEFYKTAKEAWHASYRPGQRCKLLSHPW

Specific function: Repair Of Alkylated Guanine In DNA By Stoichiometrically Transferring The Alkyl Group At The O-6 Position To A Cysteine Residue In The Enzyme. This Is A Suicide Reaction: The Enzyme Is Irreversibly Inactivated. Can Also Repair O-4-Methylthymine. The Meth

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.63 [C]

Molecular weight: Translated: 9435; Mature: 9435

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
5.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKLQRQEYYQALINKDDRYENIFFVAVKITRVFCRPTCPVRKPKFENCEFYKTAKEAW
CCCHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
HASYRPGQRCKLLSHPW
HCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFNKLQRQEYYQALINKDDRYENIFFVAVKITRVFCRPTCPVRKPKFENCEFYKTAKEAW
CCCHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
HASYRPGQRCKLLSHPW
HCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing O-methylguanine; protein L-cysteine; DNA [C]

Specific reaction: DNA containing O-methylguanine + protein L-cysteine = DNA lacking O-methylguanine + protein S-methyl-L-cysteine Protein + DNA = Protein-DNA [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA