Click here to switch to the map view.

The map label for this gene is nusA

Identifier: 15892740

GI number: 15892740

Start: 773067

End: 774578

Strand: Reverse

Name: nusA

Synonym: RC0817

Alternate gene names: 15892740

Gene position: 774578-773067 (Counterclockwise)

Preceding gene: 15892741

Following gene: 15892739

Centisome position: 61.05

GC content: 33.47

Gene sequence:

>1512_bases
ATGTCTAATATAGGTAATGTAGAAATTTTACAAATTATAGATTCTGTAGCTCGTGAGAAAGGAATATCAAAAGAAATTTT
GATTTCAACGGTTGAACAAGCGGTACAAGCAGCAGGACGAAAAAAATACGGTAATGAATATGATATTAAAGCTCAGATAA
ATAGGAAAACCGGTGAGATAAATCTTTTAAGGATCTTAAAAATAGTAGAAGATGTAGAAGATTACTTAACGCAAATCTCA
CTTGAAGAAGCTTTAATAAAGAATCCGGAAGCTAAAATCGGTGATGAGATATATGAATATTTACCGCCGATTGATCATGC
TAGAGTATCGGCTCAAGCTGCAAAACAAGTTATAACTCAACGTGTTATAGAAGCTGAACGTGAAAAACAATATCATGACT
TTAAAGATAGAAAAGGCGAAATTATAAATGGGATAGTTAAGAGAATAGAATACGGTGATATAATAGTTGACCTGAGTCGT
GCTGAAGCAATAATAAAAAAAGATCAATTAATTAAGGGTGAGAATTTTAAACCTAATGATCGTATAAAAGCATATGTACA
GGATGTAAGGCAAGAAACAAAAGGACCGCAGATTTTCTTGTCTAGAGTAGATAACCAGATGCTAGTTAAACTATTTAAGC
TAGAAGTACCTGAAATTCTTGAATCTATTATTCAAATAAAATCAGTAGCACGTGATCCCGGTTCAAAAGCAAAAATAGCG
GTATTTGCTGCGGATAGTAGTATAGATCCCGTAGGCTCATGCGTAGGTATTAGAGGTAATAGAGTAAAAGCCGTAACAAA
TGAATTAAACGGAGAGAAAATTGATATAGTATTATGGAGTAATGATCTTGCACAGTTTATAGTTAATGCTCTTGCACCGC
TTGCACCTGGGGAAATCACAAAAATTTTGATTGATGAGGATAGGCATAAGGTAGAAGTAGTAGTTTCGCAAGAAAATCAA
AGTATTGCAATAGGTAGAAGAGGGCAGAATGTTCGTTTAGCTTCTCAGCTTACAGGGTGGAATATCGATATAATGACCGA
AGAGCAAGAATCCAAAAGAAGAAATGAAGAGTTCTTAACTTCTACGGCATTATTTATGGAAGCTTTAGATGTTGAAGAAG
TTATAGGTCAGCTTTTATCGGTAACGGGATTTAATTCAGTAGAACAAATTGCTAGTAGTGAGATTAGTACTTTAACAAGA
ATTGAAGGCTTTGAGGAAGAACTTGCCGTAGAGATTAAAAATCGAGCTATTAATTACGTCGACCTTAAGAATGAAAAGAT
TATCAAAAAGCTAGAGGATCTAGGAGTTGAGCAAGAATTAATAGATATATTAGAATTATCGCTTGAATTAATATTACAAT
TTGCCGAATATGGTATAAAGACCATAGAAGATTTAGGAGAGATGAGTGTAAATGAATTTAAAAATTTAGCTCCAAATTCT
AATATAACGGACGAAAATATTAAATTGCTGATTAAAACTGCTAGACAGCATGGTGCATTGAAGGATAGTTAG

Upstream 100 bases:

>100_bases
GAACAAGAAGTACTAATTGATTATGATTTAATTAAAAATGCTAATCTAGTTTTAACTGAAGAAGTATTTAAAAAATTGCT
AAAGCAATAAGAGATAATGT

Downstream 100 bases:

>100_bases
TGGGTTGAATGTAGGGTGTATGTCATTCCTGCGAAAGCAGGCATCCAGAATAAATAGCTTAGTAATTAGGGCGTCCTTGA
AAAATAAACCTATAAAAATA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSNIGNVEILQIIDSVAREKGISKEILISTVEQAVQAAGRKKYGNEYDIKAQINRKTGEINLLRILKIVEDVEDYLTQIS
LEEALIKNPEAKIGDEIYEYLPPIDHARVSAQAAKQVITQRVIEAEREKQYHDFKDRKGEIINGIVKRIEYGDIIVDLSR
AEAIIKKDQLIKGENFKPNDRIKAYVQDVRQETKGPQIFLSRVDNQMLVKLFKLEVPEILESIIQIKSVARDPGSKAKIA
VFAADSSIDPVGSCVGIRGNRVKAVTNELNGEKIDIVLWSNDLAQFIVNALAPLAPGEITKILIDEDRHKVEVVVSQENQ
SIAIGRRGQNVRLASQLTGWNIDIMTEEQESKRRNEEFLTSTALFMEALDVEEVIGQLLSVTGFNSVEQIASSEISTLTR
IEGFEEELAVEIKNRAINYVDLKNEKIIKKLEDLGVEQELIDILELSLELILQFAEYGIKTIEDLGEMSVNEFKNLAPNS
NITDENIKLLIKTARQHGALKDS

Sequences:

>Translated_503_residues
MSNIGNVEILQIIDSVAREKGISKEILISTVEQAVQAAGRKKYGNEYDIKAQINRKTGEINLLRILKIVEDVEDYLTQIS
LEEALIKNPEAKIGDEIYEYLPPIDHARVSAQAAKQVITQRVIEAEREKQYHDFKDRKGEIINGIVKRIEYGDIIVDLSR
AEAIIKKDQLIKGENFKPNDRIKAYVQDVRQETKGPQIFLSRVDNQMLVKLFKLEVPEILESIIQIKSVARDPGSKAKIA
VFAADSSIDPVGSCVGIRGNRVKAVTNELNGEKIDIVLWSNDLAQFIVNALAPLAPGEITKILIDEDRHKVEVVVSQENQ
SIAIGRRGQNVRLASQLTGWNIDIMTEEQESKRRNEEFLTSTALFMEALDVEEVIGQLLSVTGFNSVEQIASSEISTLTR
IEGFEEELAVEIKNRAINYVDLKNEKIIKKLEDLGVEQELIDILELSLELILQFAEYGIKTIEDLGEMSVNEFKNLAPNS
NITDENIKLLIKTARQHGALKDS
>Mature_502_residues
SNIGNVEILQIIDSVAREKGISKEILISTVEQAVQAAGRKKYGNEYDIKAQINRKTGEINLLRILKIVEDVEDYLTQISL
EEALIKNPEAKIGDEIYEYLPPIDHARVSAQAAKQVITQRVIEAEREKQYHDFKDRKGEIINGIVKRIEYGDIIVDLSRA
EAIIKKDQLIKGENFKPNDRIKAYVQDVRQETKGPQIFLSRVDNQMLVKLFKLEVPEILESIIQIKSVARDPGSKAKIAV
FAADSSIDPVGSCVGIRGNRVKAVTNELNGEKIDIVLWSNDLAQFIVNALAPLAPGEITKILIDEDRHKVEVVVSQENQS
IAIGRRGQNVRLASQLTGWNIDIMTEEQESKRRNEEFLTSTALFMEALDVEEVIGQLLSVTGFNSVEQIASSEISTLTRI
EGFEEELAVEIKNRAINYVDLKNEKIIKKLEDLGVEQELIDILELSLELILQFAEYGIKTIEDLGEMSVNEFKNLAPNSN
ITDENIKLLIKTARQHGALKDS

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Escherichia coli, GI1789560, Length=491, Percent_Identity=38.6965376782077, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSA_RICCN (Q92HF4)

Other databases:

- EMBL:   AE006914
- PIR:   A97802
- RefSeq:   NP_360454.1
- ProteinModelPortal:   Q92HF4
- GeneID:   927779
- GenomeReviews:   AE006914_GR
- KEGG:   rco:RC0817
- NMPDR:   fig|272944.1.peg.817
- OMA:   KAREPWF
- ProtClustDB:   PRK09202
- BioCyc:   RCON272944:RC0817-MONOMER
- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213
- Gene3D:   G3DSA:3.30.300.20
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.30.1480.10
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR01953
- TIGRFAMs:   TIGR01954

Pfam domain/function: PF08529 NusA_N; PF00575 S1; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF47794 Rad51_N; SSF69705 TF_NusA_N

EC number: NA

Molecular weight: Translated: 56523; Mature: 56392

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIGNVEILQIIDSVAREKGISKEILISTVEQAVQAAGRKKYGNEYDIKAQINRKTGEI
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCH
NLLRILKIVEDVEDYLTQISLEEALIKNPEAKIGDEIYEYLPPIDHARVSAQAAKQVITQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RVIEAEREKQYHDFKDRKGEIINGIVKRIEYGDIIVDLSRAEAIIKKDQLIKGENFKPND
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCCCCCHH
RIKAYVQDVRQETKGPQIFLSRVDNQMLVKLFKLEVPEILESIIQIKSVARDPGSKAKIA
HHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEE
VFAADSSIDPVGSCVGIRGNRVKAVTNELNGEKIDIVLWSNDLAQFIVNALAPLAPGEIT
EEEECCCCCCCHHEECCCCCEEHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCEE
KILIDEDRHKVEVVVSQENQSIAIGRRGQNVRLASQLTGWNIDIMTEEQESKRRNEEFLT
EEEEECCCCEEEEEEECCCCEEEEECCCCCEEEHHHCCCCCEEEEECCHHHHHHHHHHHH
STALFMEALDVEEVIGQLLSVTGFNSVEQIASSEISTLTRIEGFEEELAVEIKNRAINYV
HHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEE
DLKNEKIIKKLEDLGVEQELIDILELSLELILQFAEYGIKTIEDLGEMSVNEFKNLAPNS
ECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NITDENIKLLIKTARQHGALKDS
CCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SNIGNVEILQIIDSVAREKGISKEILISTVEQAVQAAGRKKYGNEYDIKAQINRKTGEI
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCH
NLLRILKIVEDVEDYLTQISLEEALIKNPEAKIGDEIYEYLPPIDHARVSAQAAKQVITQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RVIEAEREKQYHDFKDRKGEIINGIVKRIEYGDIIVDLSRAEAIIKKDQLIKGENFKPND
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCCCCCHH
RIKAYVQDVRQETKGPQIFLSRVDNQMLVKLFKLEVPEILESIIQIKSVARDPGSKAKIA
HHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEE
VFAADSSIDPVGSCVGIRGNRVKAVTNELNGEKIDIVLWSNDLAQFIVNALAPLAPGEIT
EEEECCCCCCCHHEECCCCCEEHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCEE
KILIDEDRHKVEVVVSQENQSIAIGRRGQNVRLASQLTGWNIDIMTEEQESKRRNEEFLT
EEEEECCCCEEEEEEECCCCEEEEECCCCCEEEHHHCCCCCEEEEECCHHHHHHHHHHHH
STALFMEALDVEEVIGQLLSVTGFNSVEQIASSEISTLTRIEGFEEELAVEIKNRAINYV
HHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEE
DLKNEKIIKKLEDLGVEQELIDILELSLELILQFAEYGIKTIEDLGEMSVNEFKNLAPNS
ECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NITDENIKLLIKTARQHGALKDS
CCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893