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The map label for this gene is infB [H]

Identifier: 15892739

GI number: 15892739

Start: 770332

End: 772827

Strand: Reverse

Name: infB [H]

Synonym: RC0816

Alternate gene names: 15892739

Gene position: 772827-770332 (Counterclockwise)

Preceding gene: 15892740

Following gene: 15892738

Centisome position: 60.91

GC content: 36.26

Gene sequence:

>2496_bases
ATGACGGATAACCAGGAAATCAAACCCAAAAAGTTGACACTTGGCAATTCAAAATTATCGCTTAATAAGTCTTTTGACTC
TCTTACAGGAGCTCAAAGCTTTGTTAATGCTAAATCTAAAACGCTAGTAGAAGTTAGAAAAAGTTCTACCGGTAGTGCTA
CTACCCTTTCATTAAATAAAGAAAGGAATAGCTTAGATCAAACTGTTATTGATGCTAATAAGGAAGAATTTAACAGACGT
TTATCTATTCTTAAAAAAGCTGCTGAGCAATCTCAATTAAATGACCCTTTAAAAATAAGCACTTTAAGCAAACTTGCAAG
TATTAACCAGTCTGCTAACTCAAAAATAGAACCGCTAGAAACTGATAAAGAAGTTGAACAGAAACAGCAAAATACCGAAG
AAAATAAAGTAGAAGTTAGTGCTAAAATCGTTCAAGATGATGAAGATATACCGTCACAAATACCTAAAAAGAAAGAAGAA
ACTTTTGTAAAGTCTCCTTTAGTAGGAAAGCGAACACGTTACGGTATAGAATCAGAGAAAGAATTAGATAAAACAGCAGA
GAGTAAAATAATAGCACCAAAAATTAAGCTAGAAGAGCCAAAAAAATTCAAAAAAGCCGATCTTTTCAATATGCTTAGCG
ACGACGAAAGCGGCAGCGGTAGAACTAGAAGTCTTGCTGCTATAAAAAGAGCTAGAGAAAAAGAAAAGCGTAAATTAGTA
TCACAAGCACCTGAGAAAGTATATAGAGAAGTGACGATCCCGGAAGCTATCGGAGTCGGCGATCTTGCAAATGCTATGTC
TGAAAGAGTGGCTGATGTAATTAAAGAATTAATGAAACTCGGTATTCTTGCAAATGCTAGCCAAACAATAGATGCCGATA
CGGCAGAACTAGTTGCAACAAATTTAGGGCATACGGTAAAAAGAGTCCAAGAGTCAGATGTTGAAAATGTTTTAATTAGT
GATGATAAGGTTGAGGATTTAAGAACGCGTGCTCCGGTTGTGACGGTAATGGGTCATGTCGATCATGGTAAAACCTCATT
GCTTGACTCTCTTAAGTCTACAGATATTGCTGCAGGTGAGCTAGGCGGAATTACACAACATATTGGGGCTTATAGAGTAA
CTCTTGCAGACGGTAGAGCAATTACTTTTATTGATACTCCGGGGCATGAAGCTTTTTCGGAAATGCGGTCAAGAGGTGCT
AAAGTAACGGATATAGTTATTATAGTGGTCGCAGCAGATGATGGAATCAAAACGCAAACGGTTGAGGCAATTAATCATGC
GAAAGCAGCGGGCGTTCCTATAATTGTAGCTATTAATAAGATTGATAAGCCTGATATTGATATTGAGCGTGTAAAGAACG
AATTATATGTCCATGAAATTATCGGCGAAGAGGCAGGTGGTGACATTATGATTATTCCTATCTCGGCACTAAAGAAAATT
AACTTAGATAAACTTGAAGAAGCAATTTTATTAATTGCAGAAATGCAGGATTTAAAGGCAAATCCTTTCGGTTCAGCTGC
CGGTGTTGTAATCGAGTCAAAAATTGAACAAGGAAGAGGAACGCTAACCACTATATTAGTGCAGCGAGGTACTTTAAGAA
ACAGTGATATTATAATAGCTGGTACTGCTTACGGTAAAGTTAAAAAGATGACTAATGATAAGGGACTAGAAATAGTAGAA
GCAACTCCGTCTGTTCCTGTAGAAATTCAGGGGTTAAATGAAGTGCCTTTTGCCGGCGATAAATTTAACGTAGTACAAAA
TGAGAAACAAGCAAAAGATATAGCCGAATATAGAATGCGTCTTGCTAAAGAAAAAAAAATATCTATTGCACCGCGTTCAA
GTTTAGAAGATTTGTTTTTAAAAGCTTCCGGTAATAGTAAAATTAAGGAATTACCTTTAATTATTAAAGGTGATGTGCAA
GGCTCGGTTGAAGCTATTTCAGGTAGCTTATTAAAATTACCGAGTGATGAGATAAAGCTTCGTATACTTCATAGCGGCGT
AGGTCCGATAACGGAATCCGATGTATCTCTTGCACATGCTTCTTCGGCTATTATTGTCGGCTTTAACGTTAGAGCAGGGG
CAAATGCCTTAACTGCCGCAGAAAAAGAAAAAGTCGATATTCGATATTATAGTATAATATATCATTTAATTGATGATATA
AAAGCCATTATGAGCGGTATGCTTGATCCGATCGTTAGAGAGCAGTATATCGGAAGCGCAGAAATGAGACAAATATTTAA
TATTACCAAAGTTGGCAAAATCGCAGGTAGTTATGTAACTAAAGGGATTATTAAGAAAGGAGCGGGCGTACGCTTATTAC
GTGATAATGTAGTCATTCATGAAGGAAAACTCAAAACTTTAAAACGCTTTAAAGATGAAGTTAAAGAAGTTAGAGAAGGA
TATGAGTGTGGTATAGCATTTGAAAATTACCAAGATATTAGAGAAGGAGACACAGTTGAAGTTTTTGAACTAATTCAAGA
ACAAAGACAATTATAA

Upstream 100 bases:

>100_bases
GGAATGACATAAACGAGCCATGCGAGCAACGCCGCTTTTAGTTAGGAATGACATAATCAAGTAGTAGACGGAAAAAAACA
GAAACACAAAGGTTTTAAAT

Downstream 100 bases:

>100_bases
TATACTCGTTGAACTTCACAAATTTACATTATCATACAAGTACTGCGGCGTTGTTGCATGGATACTACTAAATCGTCATT
GCGAGTAGTTGTAGACTGCA

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 831; Mature: 830

Protein sequence:

>831_residues
MTDNQEIKPKKLTLGNSKLSLNKSFDSLTGAQSFVNAKSKTLVEVRKSSTGSATTLSLNKERNSLDQTVIDANKEEFNRR
LSILKKAAEQSQLNDPLKISTLSKLASINQSANSKIEPLETDKEVEQKQQNTEENKVEVSAKIVQDDEDIPSQIPKKKEE
TFVKSPLVGKRTRYGIESEKELDKTAESKIIAPKIKLEEPKKFKKADLFNMLSDDESGSGRTRSLAAIKRAREKEKRKLV
SQAPEKVYREVTIPEAIGVGDLANAMSERVADVIKELMKLGILANASQTIDADTAELVATNLGHTVKRVQESDVENVLIS
DDKVEDLRTRAPVVTVMGHVDHGKTSLLDSLKSTDIAAGELGGITQHIGAYRVTLADGRAITFIDTPGHEAFSEMRSRGA
KVTDIVIIVVAADDGIKTQTVEAINHAKAAGVPIIVAINKIDKPDIDIERVKNELYVHEIIGEEAGGDIMIIPISALKKI
NLDKLEEAILLIAEMQDLKANPFGSAAGVVIESKIEQGRGTLTTILVQRGTLRNSDIIIAGTAYGKVKKMTNDKGLEIVE
ATPSVPVEIQGLNEVPFAGDKFNVVQNEKQAKDIAEYRMRLAKEKKISIAPRSSLEDLFLKASGNSKIKELPLIIKGDVQ
GSVEAISGSLLKLPSDEIKLRILHSGVGPITESDVSLAHASSAIIVGFNVRAGANALTAAEKEKVDIRYYSIIYHLIDDI
KAIMSGMLDPIVREQYIGSAEMRQIFNITKVGKIAGSYVTKGIIKKGAGVRLLRDNVVIHEGKLKTLKRFKDEVKEVREG
YECGIAFENYQDIREGDTVEVFELIQEQRQL

Sequences:

>Translated_831_residues
MTDNQEIKPKKLTLGNSKLSLNKSFDSLTGAQSFVNAKSKTLVEVRKSSTGSATTLSLNKERNSLDQTVIDANKEEFNRR
LSILKKAAEQSQLNDPLKISTLSKLASINQSANSKIEPLETDKEVEQKQQNTEENKVEVSAKIVQDDEDIPSQIPKKKEE
TFVKSPLVGKRTRYGIESEKELDKTAESKIIAPKIKLEEPKKFKKADLFNMLSDDESGSGRTRSLAAIKRAREKEKRKLV
SQAPEKVYREVTIPEAIGVGDLANAMSERVADVIKELMKLGILANASQTIDADTAELVATNLGHTVKRVQESDVENVLIS
DDKVEDLRTRAPVVTVMGHVDHGKTSLLDSLKSTDIAAGELGGITQHIGAYRVTLADGRAITFIDTPGHEAFSEMRSRGA
KVTDIVIIVVAADDGIKTQTVEAINHAKAAGVPIIVAINKIDKPDIDIERVKNELYVHEIIGEEAGGDIMIIPISALKKI
NLDKLEEAILLIAEMQDLKANPFGSAAGVVIESKIEQGRGTLTTILVQRGTLRNSDIIIAGTAYGKVKKMTNDKGLEIVE
ATPSVPVEIQGLNEVPFAGDKFNVVQNEKQAKDIAEYRMRLAKEKKISIAPRSSLEDLFLKASGNSKIKELPLIIKGDVQ
GSVEAISGSLLKLPSDEIKLRILHSGVGPITESDVSLAHASSAIIVGFNVRAGANALTAAEKEKVDIRYYSIIYHLIDDI
KAIMSGMLDPIVREQYIGSAEMRQIFNITKVGKIAGSYVTKGIIKKGAGVRLLRDNVVIHEGKLKTLKRFKDEVKEVREG
YECGIAFENYQDIREGDTVEVFELIQEQRQL
>Mature_830_residues
TDNQEIKPKKLTLGNSKLSLNKSFDSLTGAQSFVNAKSKTLVEVRKSSTGSATTLSLNKERNSLDQTVIDANKEEFNRRL
SILKKAAEQSQLNDPLKISTLSKLASINQSANSKIEPLETDKEVEQKQQNTEENKVEVSAKIVQDDEDIPSQIPKKKEET
FVKSPLVGKRTRYGIESEKELDKTAESKIIAPKIKLEEPKKFKKADLFNMLSDDESGSGRTRSLAAIKRAREKEKRKLVS
QAPEKVYREVTIPEAIGVGDLANAMSERVADVIKELMKLGILANASQTIDADTAELVATNLGHTVKRVQESDVENVLISD
DKVEDLRTRAPVVTVMGHVDHGKTSLLDSLKSTDIAAGELGGITQHIGAYRVTLADGRAITFIDTPGHEAFSEMRSRGAK
VTDIVIIVVAADDGIKTQTVEAINHAKAAGVPIIVAINKIDKPDIDIERVKNELYVHEIIGEEAGGDIMIIPISALKKIN
LDKLEEAILLIAEMQDLKANPFGSAAGVVIESKIEQGRGTLTTILVQRGTLRNSDIIIAGTAYGKVKKMTNDKGLEIVEA
TPSVPVEIQGLNEVPFAGDKFNVVQNEKQAKDIAEYRMRLAKEKKISIAPRSSLEDLFLKASGNSKIKELPLIIKGDVQG
SVEAISGSLLKLPSDEIKLRILHSGVGPITESDVSLAHASSAIIVGFNVRAGANALTAAEKEKVDIRYYSIIYHLIDDIK
AIMSGMLDPIVREQYIGSAEMRQIFNITKVGKIAGSYVTKGIIKKGAGVRLLRDNVVIHEGKLKTLKRFKDEVKEVREGY
ECGIAFENYQDIREGDTVEVFELIQEQRQL

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=538, Percent_Identity=42.3791821561338, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI53729337, Length=538, Percent_Identity=42.3791821561338, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI84043963, Length=454, Percent_Identity=29.0748898678414, Blast_Score=130, Evalue=7e-30,
Organism=Homo sapiens, GI157426893, Length=247, Percent_Identity=29.5546558704453, Blast_Score=74, Evalue=6e-13,
Organism=Homo sapiens, GI94966754, Length=144, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI1789559, Length=592, Percent_Identity=53.7162162162162, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI2367247, Length=178, Percent_Identity=32.5842696629214, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1788922, Length=224, Percent_Identity=30.3571428571429, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994658, Length=542, Percent_Identity=36.90036900369, Blast_Score=348, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI212656558, Length=555, Percent_Identity=25.5855855855856, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=219, Percent_Identity=31.5068493150685, Blast_Score=86, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324550, Length=618, Percent_Identity=38.0258899676375, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319282, Length=131, Percent_Identity=40.4580152671756, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI28572034, Length=538, Percent_Identity=44.6096654275093, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI160714833, Length=75, Percent_Identity=72, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24656849, Length=131, Percent_Identity=45.0381679389313, Blast_Score=108, Evalue=2e-23,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 91074; Mature: 90942

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNQEIKPKKLTLGNSKLSLNKSFDSLTGAQSFVNAKSKTLVEVRKSSTGSATTLSLNK
CCCCCCCCCCEEEECCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEECC
ERNSLDQTVIDANKEEFNRRLSILKKAAEQSQLNDPLKISTLSKLASINQSANSKIEPLE
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCC
TDKEVEQKQQNTEENKVEVSAKIVQDDEDIPSQIPKKKEETFVKSPLVGKRTRYGIESEK
CHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHCCCCHHHHHHHCCCCCCHHHCCCCCHH
ELDKTAESKIIAPKIKLEEPKKFKKADLFNMLSDDESGSGRTRSLAAIKRAREKEKRKLV
HHHHHHHCCEECCCEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SQAPEKVYREVTIPEAIGVGDLANAMSERVADVIKELMKLGILANASQTIDADTAELVAT
HHCHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
NLGHTVKRVQESDVENVLISDDKVEDLRTRAPVVTVMGHVDHGKTSLLDSLKSTDIAAGE
HHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCHH
LGGITQHIGAYRVTLADGRAITFIDTPGHEAFSEMRSRGAKVTDIVIIVVAADDGIKTQT
HCCHHHHCCCEEEEEECCCEEEEEECCCHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHH
VEAINHAKAAGVPIIVAINKIDKPDIDIERVKNELYVHEIIGEEAGGDIMIIPISALKKI
HHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHC
NLDKLEEAILLIAEMQDLKANPFGSAAGVVIESKIEQGRGTLTTILVQRGTLRNSDIIIA
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHCCCCCEEEEEEECCCCCCCCEEEE
GTAYGKVKKMTNDKGLEIVEATPSVPVEIQGLNEVPFAGDKFNVVQNEKQAKDIAEYRMR
ECCCHHHHHCCCCCCCEEEECCCCCCEEECCCCCCCCCCCCCCHHCCHHHHHHHHHHHHH
LAKEKKISIAPRSSLEDLFLKASGNSKIKELPLIIKGDVQGSVEAISGSLLKLPSDEIKL
HHHCCCEEECCCCCCCHHEEECCCCCCHHHCCEEEECCCCCCHHHHCCCEEECCCCCEEE
RILHSGVGPITESDVSLAHASSAIIVGFNVRAGANALTAAEKEKVDIRYYSIIYHLIDDI
EEEECCCCCCCCCCCHHHCCCCEEEEEEECCCCCCHHHHCHHHCCHHHHHHHHHHHHHHH
KAIMSGMLDPIVREQYIGSAEMRQIFNITKVGKIAGSYVTKGIIKKGAGVRLLRDNVVIH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEE
EGKLKTLKRFKDEVKEVREGYECGIAFENYQDIREGDTVEVFELIQEQRQL
CCHHHHHHHHHHHHHHHHCCHHCCEEECCHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDNQEIKPKKLTLGNSKLSLNKSFDSLTGAQSFVNAKSKTLVEVRKSSTGSATTLSLNK
CCCCCCCCCEEEECCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEECC
ERNSLDQTVIDANKEEFNRRLSILKKAAEQSQLNDPLKISTLSKLASINQSANSKIEPLE
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCC
TDKEVEQKQQNTEENKVEVSAKIVQDDEDIPSQIPKKKEETFVKSPLVGKRTRYGIESEK
CHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHCCCCHHHHHHHCCCCCCHHHCCCCCHH
ELDKTAESKIIAPKIKLEEPKKFKKADLFNMLSDDESGSGRTRSLAAIKRAREKEKRKLV
HHHHHHHCCEECCCEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SQAPEKVYREVTIPEAIGVGDLANAMSERVADVIKELMKLGILANASQTIDADTAELVAT
HHCHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
NLGHTVKRVQESDVENVLISDDKVEDLRTRAPVVTVMGHVDHGKTSLLDSLKSTDIAAGE
HHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCHH
LGGITQHIGAYRVTLADGRAITFIDTPGHEAFSEMRSRGAKVTDIVIIVVAADDGIKTQT
HCCHHHHCCCEEEEEECCCEEEEEECCCHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHH
VEAINHAKAAGVPIIVAINKIDKPDIDIERVKNELYVHEIIGEEAGGDIMIIPISALKKI
HHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHC
NLDKLEEAILLIAEMQDLKANPFGSAAGVVIESKIEQGRGTLTTILVQRGTLRNSDIIIA
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHCCCCCEEEEEEECCCCCCCCEEEE
GTAYGKVKKMTNDKGLEIVEATPSVPVEIQGLNEVPFAGDKFNVVQNEKQAKDIAEYRMR
ECCCHHHHHCCCCCCCEEEECCCCCCEEECCCCCCCCCCCCCCHHCCHHHHHHHHHHHHH
LAKEKKISIAPRSSLEDLFLKASGNSKIKELPLIIKGDVQGSVEAISGSLLKLPSDEIKL
HHHCCCEEECCCCCCCHHEEECCCCCCHHHCCEEEECCCCCCHHHHCCCEEECCCCCEEE
RILHSGVGPITESDVSLAHASSAIIVGFNVRAGANALTAAEKEKVDIRYYSIIYHLIDDI
EEEECCCCCCCCCCCHHHCCCCEEEEEEECCCCCCHHHHCHHHCCHHHHHHHHHHHHHHH
KAIMSGMLDPIVREQYIGSAEMRQIFNITKVGKIAGSYVTKGIIKKGAGVRLLRDNVVIH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEE
EGKLKTLKRFKDEVKEVREGYECGIAFENYQDIREGDTVEVFELIQEQRQL
CCHHHHHHHHHHHHHHHHCCHHCCEEECCHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA