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The map label for this gene is priA

Identifier: 15892723

GI number: 15892723

Start: 754229

End: 756175

Strand: Reverse

Name: priA

Synonym: RC0800

Alternate gene names: 15892723

Gene position: 756175-754229 (Counterclockwise)

Preceding gene: 15892725

Following gene: 15892721

Centisome position: 59.6

GC content: 33.23

Gene sequence:

>1947_bases
ATGCGAATAGCAAAAATATTATTACCGGTAGCAAAATTATTTCCGCTAGATTATTTAATACCTGAAGATTTAGAGCTAAA
CATAGGTGATCTTGTTGTAGTGCCTTTTAGAAATAAGGAATTAACCGGCATAGTATGGGAGCTTGTTTCAAATTCCGAGG
CAAAAAAAATAAAAACTATTAGAGTGAAAGTACCGTTAAACTTAAATATTACTTCAGAAGTTTTAGAATTAATTAAATGG
ATGAGTAGCTACTATATGTCAGAGCTTGGGAGCATAGCCAAGTTAGTATTACCTATGGATATTGCTGAAAAACCGATTAA
AGTTAAGGAGCAGAAAGTCAATAATAACTTTGTGCTACCTGATTTGTCGGAAGAGCAGAAACAAGCAGTAACAATTTTAA
ATGAAAGTAATAAGCCAACACTTGTTAAAGGTGTTACGGGATCAGGTAAAACAGAAATATATTTTCATCTAATAGCAGAT
TACTTAGCAAAAGGCAAACAAGTGCTTGTTATGTTGCCTGAAATTGCTTTAAGTACACAAATTATTAATCGTTTTATAGA
GCGATTCGGTTTTGAGCCTATCATATGGAACTCAAGCGTTACCAAAGCTCACAAGAAAATGATTTTAAGAGGTATATTAA
GCGATAAAGTTAAAGTAGTAATTGGGGCTAGAAGTAGTTTGTTTTTACCTTTTAAAAATCTCGGTTTAATAGTTATAGAT
GAGGAACATGACGATTCCTATAAACAAGATGACGGTATATTATATAATGCTAGGGATACGGCTATCGTAAGAGGTACATT
TGATAAAGCACAAATTGTTTTGTGTTCGGCAACACCGTCTATTGAAACGATACATAATATAGAAATTGGTAAATATCAAT
TGGTTACATTGGTTAATAGATACAAAAATGTCGATTTGCCAAATATAGAAATAATCGACATGACTAAAGAAAAGCTATCT
AAGAATTCCTATTTATCCAAGCTTCTTATAGAAGCTATCAAAGGTAATTTAGATAATAAAAAGCAAGTCTTATTATTTCT
TAATAGGCGTGGTTATGCTCCGCTCATGCTATGCAAAGCTTGCGGTTACAGATTGACCTGTAAATTCTGCTCTTCTTGGA
TGGTAGTACATAAAGCGACTAAAAAACTTGAATGTCACCATTGCGGTTATCAAAGTAAAATTTTTAGTTCTTGTCCTGAA
TGTCTAGAGGATGAAACATTAACTATCTGTGGTCCAGGTATAGAGAGAATAGAGGAAGAAGCAAAAGCACTTTTTCCTGA
GAGTAAAATTGCGGTGATAAGTAAAGATCATGCTAAAAGCCCGGAAAAAATAGCACAGCTTTTACATCAAATGGAAAATT
TAGAAATTGATATTTTAATAGGCACACAAATAATAACAAAAGGTTATCACTTTCCGAATCTTACTTTAGTTGGAGTAATA
GATGCCGATCTTGGAAGCAATAATGCCGATCTTAGAGCATCTGAGCGCACTTTTCAGCTACTACATCAGGTAGGTGGTAG
AGCGGGTAGAGGAGATAGTAAAGGTGTAGTATATTTACAAAGCTATTATCCTGATAATACAATTTTTAGTTATGTTAAAG
CCGGTGATGAAGATAGTTTTTTTGCAAATGAACTTGAAATAAGAAAATCAGCAGATATGCCGCCATTTTCCAAAACAGCA
TCGGTAATTTTGTCAGGTTCCAGTGAGGCTAAAATTCTAGAAATTGCTAGGGATATGGTCCGAATTGCACCAAAAGCAAA
CGTGAAAATTTTAGGACCGGCAAGCTCATTAATGTCAAAGCTCGCTGGTAAATATCGTTACCGAATACTTATTATAGCCA
ATAAGAAATTTAATTTGCAGAAATATTTAAAATTTTGGCTAAGCCTTATAAAAATTCCTTCTTTTTGCCATCTAAAAATA
GATATCGATCCTAAAAGTTTTTATTAG

Upstream 100 bases:

>100_bases
CAGAAATTGCTATAACAATTTTTGTTTCTTTATTCATCTAGGTTTCTATAATTTATCAGTTTAGTATATAATAAAAAAAA
AATTATAAAATACATTATAT

Downstream 100 bases:

>100_bases
CTTCTATATCTTTCTCCTTGATTCTTCGACGGTGGAACATTAGATGAATGAGGAGAAGGATTGGTAAGAGATTTTTGTAT
TTCTGCTATAGCTTTTTTAG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MRIAKILLPVAKLFPLDYLIPEDLELNIGDLVVVPFRNKELTGIVWELVSNSEAKKIKTIRVKVPLNLNITSEVLELIKW
MSSYYMSELGSIAKLVLPMDIAEKPIKVKEQKVNNNFVLPDLSEEQKQAVTILNESNKPTLVKGVTGSGKTEIYFHLIAD
YLAKGKQVLVMLPEIALSTQIINRFIERFGFEPIIWNSSVTKAHKKMILRGILSDKVKVVIGARSSLFLPFKNLGLIVID
EEHDDSYKQDDGILYNARDTAIVRGTFDKAQIVLCSATPSIETIHNIEIGKYQLVTLVNRYKNVDLPNIEIIDMTKEKLS
KNSYLSKLLIEAIKGNLDNKKQVLLFLNRRGYAPLMLCKACGYRLTCKFCSSWMVVHKATKKLECHHCGYQSKIFSSCPE
CLEDETLTICGPGIERIEEEAKALFPESKIAVISKDHAKSPEKIAQLLHQMENLEIDILIGTQIITKGYHFPNLTLVGVI
DADLGSNNADLRASERTFQLLHQVGGRAGRGDSKGVVYLQSYYPDNTIFSYVKAGDEDSFFANELEIRKSADMPPFSKTA
SVILSGSSEAKILEIARDMVRIAPKANVKILGPASSLMSKLAGKYRYRILIIANKKFNLQKYLKFWLSLIKIPSFCHLKI
DIDPKSFY

Sequences:

>Translated_648_residues
MRIAKILLPVAKLFPLDYLIPEDLELNIGDLVVVPFRNKELTGIVWELVSNSEAKKIKTIRVKVPLNLNITSEVLELIKW
MSSYYMSELGSIAKLVLPMDIAEKPIKVKEQKVNNNFVLPDLSEEQKQAVTILNESNKPTLVKGVTGSGKTEIYFHLIAD
YLAKGKQVLVMLPEIALSTQIINRFIERFGFEPIIWNSSVTKAHKKMILRGILSDKVKVVIGARSSLFLPFKNLGLIVID
EEHDDSYKQDDGILYNARDTAIVRGTFDKAQIVLCSATPSIETIHNIEIGKYQLVTLVNRYKNVDLPNIEIIDMTKEKLS
KNSYLSKLLIEAIKGNLDNKKQVLLFLNRRGYAPLMLCKACGYRLTCKFCSSWMVVHKATKKLECHHCGYQSKIFSSCPE
CLEDETLTICGPGIERIEEEAKALFPESKIAVISKDHAKSPEKIAQLLHQMENLEIDILIGTQIITKGYHFPNLTLVGVI
DADLGSNNADLRASERTFQLLHQVGGRAGRGDSKGVVYLQSYYPDNTIFSYVKAGDEDSFFANELEIRKSADMPPFSKTA
SVILSGSSEAKILEIARDMVRIAPKANVKILGPASSLMSKLAGKYRYRILIIANKKFNLQKYLKFWLSLIKIPSFCHLKI
DIDPKSFY
>Mature_648_residues
MRIAKILLPVAKLFPLDYLIPEDLELNIGDLVVVPFRNKELTGIVWELVSNSEAKKIKTIRVKVPLNLNITSEVLELIKW
MSSYYMSELGSIAKLVLPMDIAEKPIKVKEQKVNNNFVLPDLSEEQKQAVTILNESNKPTLVKGVTGSGKTEIYFHLIAD
YLAKGKQVLVMLPEIALSTQIINRFIERFGFEPIIWNSSVTKAHKKMILRGILSDKVKVVIGARSSLFLPFKNLGLIVID
EEHDDSYKQDDGILYNARDTAIVRGTFDKAQIVLCSATPSIETIHNIEIGKYQLVTLVNRYKNVDLPNIEIIDMTKEKLS
KNSYLSKLLIEAIKGNLDNKKQVLLFLNRRGYAPLMLCKACGYRLTCKFCSSWMVVHKATKKLECHHCGYQSKIFSSCPE
CLEDETLTICGPGIERIEEEAKALFPESKIAVISKDHAKSPEKIAQLLHQMENLEIDILIGTQIITKGYHFPNLTLVGVI
DADLGSNNADLRASERTFQLLHQVGGRAGRGDSKGVVYLQSYYPDNTIFSYVKAGDEDSFFANELEIRKSADMPPFSKTA
SVILSGSSEAKILEIARDMVRIAPKANVKILGPASSLMSKLAGKYRYRILIIANKKFNLQKYLKFWLSLIKIPSFCHLKI
DIDPKSFY

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI1790370, Length=536, Percent_Identity=36.9402985074627, Blast_Score=357, Evalue=2e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIA_RICCN (Q92HH1)

Other databases:

- EMBL:   AE006914
- PIR:   H97799
- RefSeq:   NP_360437.1
- ProteinModelPortal:   Q92HH1
- SMR:   Q92HH1
- GeneID:   927613
- GenomeReviews:   AE006914_GR
- KEGG:   rco:RC0800
- NMPDR:   fig|272944.1.peg.800
- OMA:   TEVYLHV
- ProtClustDB:   PRK05580
- BioCyc:   RCON272944:RC0800-MONOMER
- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00595

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C

EC number: NA

Molecular weight: Translated: 72950; Mature: 72950

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAKILLPVAKLFPLDYLIPEDLELNIGDLVVVPFRNKELTGIVWELVSNSEAKKIKTI
CCHHHHHHHHHHHCCCCCCCCCCCEECCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEE
RVKVPLNLNITSEVLELIKWMSSYYMSELGSIAKLVLPMDIAEKPIKVKEQKVNNNFVLP
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEECC
DLSEEQKQAVTILNESNKPTLVKGVTGSGKTEIYFHLIADYLAKGKQVLVMLPEIALSTQ
CCCHHHHHEEEEEECCCCCEEEEECCCCCCCEEHHHHHHHHHCCCCEEEEEECHHHHHHH
IINRFIERFGFEPIIWNSSVTKAHKKMILRGILSDKVKVVIGARSSLFLPFKNLGLIVID
HHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEHHHCCEEEEE
EEHDDSYKQDDGILYNARDTAIVRGTFDKAQIVLCSATPSIETIHNIEIGKYQLVTLVNR
CCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEEEECCCCCCCEECCCCCCCHHHHHHHH
YKNVDLPNIEIIDMTKEKLSKNSYLSKLLIEAIKGNLDNKKQVLLFLNRRGYAPLMLCKA
HCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHH
CGYRLTCKFCSSWMVVHKATKKLECHHCGYQSKIFSSCPECLEDETLTICGPGIERIEEE
CCCEEEEHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHCHHHHCCCEEEEECCCHHHHHHH
AKALFPESKIAVISKDHAKSPEKIAQLLHQMENLEIDILIGTQIITKGYHFPNLTLVGVI
HHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHCCCCCCCEEEEEEE
DADLGSNNADLRASERTFQLLHQVGGRAGRGDSKGVVYLQSYYPDNTIFSYVKAGDEDSF
ECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCE
FANELEIRKSADMPPFSKTASVILSGSSEAKILEIARDMVRIAPKANVKILGPASSLMSK
EHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
LAGKYRYRILIIANKKFNLQKYLKFWLSLIKIPSFCHLKIDIDPKSFY
HCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MRIAKILLPVAKLFPLDYLIPEDLELNIGDLVVVPFRNKELTGIVWELVSNSEAKKIKTI
CCHHHHHHHHHHHCCCCCCCCCCCEECCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEE
RVKVPLNLNITSEVLELIKWMSSYYMSELGSIAKLVLPMDIAEKPIKVKEQKVNNNFVLP
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEECC
DLSEEQKQAVTILNESNKPTLVKGVTGSGKTEIYFHLIADYLAKGKQVLVMLPEIALSTQ
CCCHHHHHEEEEEECCCCCEEEEECCCCCCCEEHHHHHHHHHCCCCEEEEEECHHHHHHH
IINRFIERFGFEPIIWNSSVTKAHKKMILRGILSDKVKVVIGARSSLFLPFKNLGLIVID
HHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEHHHCCEEEEE
EEHDDSYKQDDGILYNARDTAIVRGTFDKAQIVLCSATPSIETIHNIEIGKYQLVTLVNR
CCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEEEECCCCCCCEECCCCCCCHHHHHHHH
YKNVDLPNIEIIDMTKEKLSKNSYLSKLLIEAIKGNLDNKKQVLLFLNRRGYAPLMLCKA
HCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHH
CGYRLTCKFCSSWMVVHKATKKLECHHCGYQSKIFSSCPECLEDETLTICGPGIERIEEE
CCCEEEEHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHCHHHHCCCEEEEECCCHHHHHHH
AKALFPESKIAVISKDHAKSPEKIAQLLHQMENLEIDILIGTQIITKGYHFPNLTLVGVI
HHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHCCCCCCCEEEEEEE
DADLGSNNADLRASERTFQLLHQVGGRAGRGDSKGVVYLQSYYPDNTIFSYVKAGDEDSF
ECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCE
FANELEIRKSADMPPFSKTASVILSGSSEAKILEIARDMVRIAPKANVKILGPASSLMSK
EHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
LAGKYRYRILIIANKKFNLQKYLKFWLSLIKIPSFCHLKIDIDPKSFY
HCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893