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The map label for this gene is sppA [H]

Identifier: 15892681

GI number: 15892681

Start: 720896

End: 721810

Strand: Reverse

Name: sppA [H]

Synonym: RC0758

Alternate gene names: 15892681

Gene position: 721810-720896 (Counterclockwise)

Preceding gene: 15892682

Following gene: 15892680

Centisome position: 56.89

GC content: 34.1

Gene sequence:

>915_bases
ATGAGTATAACACCTGATTATTTAATTGAAAGAAGACAAATAAAATCTCGTTTGTTAATTTGGAAGCTAGCAGCTATTAT
TTTAATTGCGATCGTATTCTTACTAGTCGGTAAAGATTTTGCTCCAAAAGAGGTGTTACCTATTAATAGTAATGAAGACT
ATATAGCTTCCGTATTAATAGACGAAATAATTCTTGAAGACGAAAAGCGTGACAAAAAACTCAAAAAAATAATCGATGAT
TCTCATATTAAAGCGTTAATAGTTAACGTAAATTCTCCCGGCGGTACGGTAGTCGGCGCTGAAAAAATTTATAATATTCT
GCGTAAAATATCCGCAAAAAAGCCGGTAGTGATAGTTATGGGAACAATGGCTGCAAGCGGTGGTTATTTAATCTCTCTTG
GAGGTGATTATATTATCAGCCATAACGGAACTATTACGGGTTCAATCGGTGTAATACTACAAACAGCCGAGGTAACGGAG
CTTGCTCAAAAATTGGGGATTAAGTTTAATAATTTCAAATCGGGTGAATTAAAAGCTGTCCCAAATCCTACCGAAAAACT
GACGGAAGCAGTGCGGGTAGCCATTATGGACAATATAGAAGATACTTATAATTTCTTCCTTGAACTTGTTTCAGAGAGGC
GTAATCTTCCTATAGAAGAAGTAAAAAAGCTCGCAGACGGACGAGTATATTCAGGTCGTCAGGCTTTAAAGTTAAAGCTT
GTGGATGCTATAGGCTCAGAAGATACTGCATTAAAATGGTTACAGGAAGTTCAGAAAATTAATGTTAATCTACTAGTTAA
AGATTATCAATTAAAACCAAAACCAAAATTAATGGACATAATACTTGAAGATTTTGATAGTATAGCACCTAGTTTTTTTA
AAAATAGTTTTAACGGAATCAAGGCAATTTTTTAA

Upstream 100 bases:

>100_bases
TTGTGCTTCGTTGTGCGTCGTTATTATATTATAGGCTTCGCTCCTCACGCCTGTCACAGTATAGGTCTGCATTTAGTATT
CTAAAAAAGTATATAAGATC

Downstream 100 bases:

>100_bases
TAATGATTACTAAGAATTATTTAATAGATAAAATACATGATAAGCTGAATTGTCTTTCTAAAGAAGATGTTAAAGATTCA
GTAGATTTAATTTTAGATTA

Product: protease IV

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSITPDYLIERRQIKSRLLIWKLAAIILIAIVFLLVGKDFAPKEVLPINSNEDYIASVLIDEIILEDEKRDKKLKKIIDD
SHIKALIVNVNSPGGTVVGAEKIYNILRKISAKKPVVIVMGTMAASGGYLISLGGDYIISHNGTITGSIGVILQTAEVTE
LAQKLGIKFNNFKSGELKAVPNPTEKLTEAVRVAIMDNIEDTYNFFLELVSERRNLPIEEVKKLADGRVYSGRQALKLKL
VDAIGSEDTALKWLQEVQKINVNLLVKDYQLKPKPKLMDIILEDFDSIAPSFFKNSFNGIKAIF

Sequences:

>Translated_304_residues
MSITPDYLIERRQIKSRLLIWKLAAIILIAIVFLLVGKDFAPKEVLPINSNEDYIASVLIDEIILEDEKRDKKLKKIIDD
SHIKALIVNVNSPGGTVVGAEKIYNILRKISAKKPVVIVMGTMAASGGYLISLGGDYIISHNGTITGSIGVILQTAEVTE
LAQKLGIKFNNFKSGELKAVPNPTEKLTEAVRVAIMDNIEDTYNFFLELVSERRNLPIEEVKKLADGRVYSGRQALKLKL
VDAIGSEDTALKWLQEVQKINVNLLVKDYQLKPKPKLMDIILEDFDSIAPSFFKNSFNGIKAIF
>Mature_303_residues
SITPDYLIERRQIKSRLLIWKLAAIILIAIVFLLVGKDFAPKEVLPINSNEDYIASVLIDEIILEDEKRDKKLKKIIDDS
HIKALIVNVNSPGGTVVGAEKIYNILRKISAKKPVVIVMGTMAASGGYLISLGGDYIISHNGTITGSIGVILQTAEVTEL
AQKLGIKFNNFKSGELKAVPNPTEKLTEAVRVAIMDNIEDTYNFFLELVSERRNLPIEEVKKLADGRVYSGRQALKLKLV
DAIGSEDTALKWLQEVQKINVNLLVKDYQLKPKPKLMDIILEDFDSIAPSFFKNSFNGIKAIF

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=235, Percent_Identity=31.4893617021277, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787527, Length=184, Percent_Identity=27.1739130434783, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 33852; Mature: 33721

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITPDYLIERRQIKSRLLIWKLAAIILIAIVFLLVGKDFAPKEVLPINSNEDYIASVLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHH
DEIILEDEKRDKKLKKIIDDSHIKALIVNVNSPGGTVVGAEKIYNILRKISAKKPVVIVM
HHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEHHHHHHHHHHHCCCCCEEEEE
GTMAASGGYLISLGGDYIISHNGTITGSIGVILQTAEVTELAQKLGIKFNNFKSGELKAV
ECEECCCCEEEEECCCEEEECCCEEEECCEEEEEHHHHHHHHHHHCCEECCCCCCCEEEC
PNPTEKLTEAVRVAIMDNIEDTYNFFLELVSERRNLPIEEVKKLADGRVYSGRQALKLKL
CCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCHHHEEEE
VDAIGSEDTALKWLQEVQKINVNLLVKDYQLKPKPKLMDIILEDFDSIAPSFFKNSFNGI
HHHCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHCCCCC
KAIF
EECC
>Mature Secondary Structure 
SITPDYLIERRQIKSRLLIWKLAAIILIAIVFLLVGKDFAPKEVLPINSNEDYIASVLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHH
DEIILEDEKRDKKLKKIIDDSHIKALIVNVNSPGGTVVGAEKIYNILRKISAKKPVVIVM
HHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEHHHHHHHHHHHCCCCCEEEEE
GTMAASGGYLISLGGDYIISHNGTITGSIGVILQTAEVTELAQKLGIKFNNFKSGELKAV
ECEECCCCEEEEECCCEEEECCCEEEECCEEEEEHHHHHHHHHHHCCEECCCCCCCEEEC
PNPTEKLTEAVRVAIMDNIEDTYNFFLELVSERRNLPIEEVKKLADGRVYSGRQALKLKL
CCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCHHHEEEE
VDAIGSEDTALKWLQEVQKINVNLLVKDYQLKPKPKLMDIILEDFDSIAPSFFKNSFNGI
HHHCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHCCCCC
KAIF
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123 [H]