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The map label for this gene is potE [H]

Identifier: 15892649

GI number: 15892649

Start: 698862

End: 700145

Strand: Reverse

Name: potE [H]

Synonym: RC0726

Alternate gene names: 15892649

Gene position: 700145-698862 (Counterclockwise)

Preceding gene: 15892651

Following gene: 15892648

Centisome position: 55.18

GC content: 36.06

Gene sequence:

>1284_bases
ATGTCACAAAAATTAGGTTTCTGGGCGGTTTTTGCCTTAGTAACCGGCAGTCAAATCGGTACTAGCGTTTTTATATTGCC
GTTAAGTTTAGCACCGTTCGGTATATACAGCATTTGGGGCTGGGTACTTTCATTATTCGGTGCTATGAGTATAGCACTTG
TATTTTCTACCCTTTGTGCAAAATTTCCTAAAACAGGAGGTCCGCACGTTTATGTACGGGAAAATTTTGGGGATAAAATA
GCTTTTTTCACCGGTTGGACTTACTGGGTTATATCCTTTGTCAGTACAAGTATAGTAGTCATTTCGGCAATAGGTTATCT
AACGCCTTTTTTTAAATCACAAGCGATTTTAGACTTAATATTACAGATCATATTATTAGGTGCTATTATGGTTTTAAATT
TAAAAGGTCCTGAAGTAGCAGGAAAAGCAGAGTTTTATTTAACTCTCTTAAAATTTGTTCCATTGCTTATAGTAGGTTTA
TGTGCTTTATCACATTTTAATATAGATAATATAACTATTGCCGAGGAAGTAGAGAATTTAAGCATTCCGAGCATTATGGG
AAGAGTTGTACTTCTTACTTTTTGGGGATTTATCGGCATAGAATGTGCAACTACCACGGCAGGTGCGGTAAAAGATCCGG
CAAAAACCATTCCACGAGCCATAATAGTTGGAACTTTTTGCGTAGCAGTGTTATATATTATTAATAGCATAGGTATAATG
GGATTAATCCCTGCTTCTGAACTTGTCAGTGCTAAAGCTCCTTATGCCGATGCCGCTTCATTATTATTCGGGGGTAAATG
GTCAAGCGTAATTACGGTTATAGCCTCGATTATATGTATAGGTACGCTCAATGCTTGGGTGCTAACTAGCGGACAGATAG
CTCTGGGGCTTGCAGAAGACGGTTTATTACCGAAATTTTTTGCTAAGAAAAATAGCAATAACGCTCCAACTCACGGAATT
ATTGTAAGCTGCCTTGGCATTGTGCCGTTATTAGTCTTTACGGCAAATGATAATTTTGCCAAACAGATTACACAAATAAT
AGATTTCTCGGCAATCACATTCTTATTCGTCTATTTAATTTGTAGCTTAGCTTTTTTAAAGGTGATTTTTAGCTCAAAAG
AAAATTTTTCTTATTATTATTTACTGATAGCTATAATATCGATTATCTTTTGTGCATGGGTTATCTATGAAACACCTGTT
AAAACTCTGATTATAGCGAGTGCTTTTACTATCTTTGGTATTCCTTTGTATTATGGATGGTATAAATGTCATTCCCGTCT
ATGA

Upstream 100 bases:

>100_bases
TTGTTTAGAAATCAAGAGTAAAATAATTATAGTAGTTTTAACTATTGCTAAAAATCATTATTTCAGCTATGTTAAAGCTG
TTTCTTTAAATTAGATTAAT

Downstream 100 bases:

>100_bases
GCGTTGCCTAGCTGGATGCTGAGCTGTCATTGCGAGCGACTGCAAGGAGCGTGGCAATCCAGAAAAAATAATAAAAAAAT
TCTGTAAATCAGAATTTTTT

Product: putrescine-ornithine antiporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSQKLGFWAVFALVTGSQIGTSVFILPLSLAPFGIYSIWGWVLSLFGAMSIALVFSTLCAKFPKTGGPHVYVRENFGDKI
AFFTGWTYWVISFVSTSIVVISAIGYLTPFFKSQAILDLILQIILLGAIMVLNLKGPEVAGKAEFYLTLLKFVPLLIVGL
CALSHFNIDNITIAEEVENLSIPSIMGRVVLLTFWGFIGIECATTTAGAVKDPAKTIPRAIIVGTFCVAVLYIINSIGIM
GLIPASELVSAKAPYADAASLLFGGKWSSVITVIASIICIGTLNAWVLTSGQIALGLAEDGLLPKFFAKKNSNNAPTHGI
IVSCLGIVPLLVFTANDNFAKQITQIIDFSAITFLFVYLICSLAFLKVIFSSKENFSYYYLLIAIISIIFCAWVIYETPV
KTLIIASAFTIFGIPLYYGWYKCHSRL

Sequences:

>Translated_427_residues
MSQKLGFWAVFALVTGSQIGTSVFILPLSLAPFGIYSIWGWVLSLFGAMSIALVFSTLCAKFPKTGGPHVYVRENFGDKI
AFFTGWTYWVISFVSTSIVVISAIGYLTPFFKSQAILDLILQIILLGAIMVLNLKGPEVAGKAEFYLTLLKFVPLLIVGL
CALSHFNIDNITIAEEVENLSIPSIMGRVVLLTFWGFIGIECATTTAGAVKDPAKTIPRAIIVGTFCVAVLYIINSIGIM
GLIPASELVSAKAPYADAASLLFGGKWSSVITVIASIICIGTLNAWVLTSGQIALGLAEDGLLPKFFAKKNSNNAPTHGI
IVSCLGIVPLLVFTANDNFAKQITQIIDFSAITFLFVYLICSLAFLKVIFSSKENFSYYYLLIAIISIIFCAWVIYETPV
KTLIIASAFTIFGIPLYYGWYKCHSRL
>Mature_426_residues
SQKLGFWAVFALVTGSQIGTSVFILPLSLAPFGIYSIWGWVLSLFGAMSIALVFSTLCAKFPKTGGPHVYVRENFGDKIA
FFTGWTYWVISFVSTSIVVISAIGYLTPFFKSQAILDLILQIILLGAIMVLNLKGPEVAGKAEFYLTLLKFVPLLIVGLC
ALSHFNIDNITIAEEVENLSIPSIMGRVVLLTFWGFIGIECATTTAGAVKDPAKTIPRAIIVGTFCVAVLYIINSIGIMG
LIPASELVSAKAPYADAASLLFGGKWSSVITVIASIICIGTLNAWVLTSGQIALGLAEDGLLPKFFAKKNSNNAPTHGII
VSCLGIVPLLVFTANDNFAKQITQIIDFSAITFLFVYLICSLAFLKVIFSSKENFSYYYLLIAIISIIFCAWVIYETPVK
TLIIASAFTIFGIPLYYGWYKCHSRL

Specific function: Major component of the acid-resistance (AR) system allowing enteric pathogens to survive the acidic environment in the stomach. Exchanges extracellular arginine for its intracellular decarboxylation product agmatine (Agm) thereby expelling intracellular p

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI7657683, Length=293, Percent_Identity=26.6211604095563, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI2367353, Length=405, Percent_Identity=27.9012345679012, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1786908, Length=424, Percent_Identity=24.0566037735849, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1790575, Length=430, Percent_Identity=24.8837209302326, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87082250, Length=454, Percent_Identity=25.5506607929515, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787890, Length=361, Percent_Identity=24.6537396121884, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1790653, Length=311, Percent_Identity=28.2958199356913, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1790584, Length=372, Percent_Identity=22.0430107526882, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081915, Length=397, Percent_Identity=23.9294710327456, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786602, Length=313, Percent_Identity=23.961661341853, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786302, Length=319, Percent_Identity=21.3166144200627, Blast_Score=62, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI32566699, Length=354, Percent_Identity=24.8587570621469, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45550968, Length=466, Percent_Identity=23.175965665236, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921172, Length=466, Percent_Identity=23.175965665236, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357367, Length=451, Percent_Identity=24.1685144124169, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221331183, Length=446, Percent_Identity=24.8878923766816, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17647653, Length=446, Percent_Identity=24.8878923766816, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24664379, Length=446, Percent_Identity=24.8878923766816, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 46403; Mature: 46272

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKLGFWAVFALVTGSQIGTSVFILPLSLAPFGIYSIWGWVLSLFGAMSIALVFSTLCA
CCCCHHHHHHHHHHHCCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFPKTGGPHVYVRENFGDKIAFFTGWTYWVISFVSTSIVVISAIGYLTPFFKSQAILDLI
HCCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQIILLGAIMVLNLKGPEVAGKAEFYLTLLKFVPLLIVGLCALSHFNIDNITIAEEVENL
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHCC
SIPSIMGRVVLLTFWGFIGIECATTTAGAVKDPAKTIPRAIIVGTFCVAVLYIINSIGIM
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
GLIPASELVSAKAPYADAASLLFGGKWSSVITVIASIICIGTLNAWVLTSGQIALGLAED
EECCHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHEEECCCEEEEECCC
GLLPKFFAKKNSNNAPTHGIIVSCLGIVPLLVFTANDNFAKQITQIIDFSAITFLFVYLI
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHH
CSLAFLKVIFSSKENFSYYYLLIAIISIIFCAWVIYETPVKTLIIASAFTIFGIPLYYGW
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
YKCHSRL
HHHHCCC
>Mature Secondary Structure 
SQKLGFWAVFALVTGSQIGTSVFILPLSLAPFGIYSIWGWVLSLFGAMSIALVFSTLCA
CCCHHHHHHHHHHHCCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFPKTGGPHVYVRENFGDKIAFFTGWTYWVISFVSTSIVVISAIGYLTPFFKSQAILDLI
HCCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQIILLGAIMVLNLKGPEVAGKAEFYLTLLKFVPLLIVGLCALSHFNIDNITIAEEVENL
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHCC
SIPSIMGRVVLLTFWGFIGIECATTTAGAVKDPAKTIPRAIIVGTFCVAVLYIINSIGIM
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
GLIPASELVSAKAPYADAASLLFGGKWSSVITVIASIICIGTLNAWVLTSGQIALGLAED
EECCHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHEEECCCEEEEECCC
GLLPKFFAKKNSNNAPTHGIIVSCLGIVPLLVFTANDNFAKQITQIIDFSAITFLFVYLI
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHH
CSLAFLKVIFSSKENFSYYYLLIAIISIIFCAWVIYETPVKTLIIASAFTIFGIPLYYGW
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
YKCHSRL
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]