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The map label for this gene is puuA [H]

Identifier: 15892594

GI number: 15892594

Start: 653305

End: 654138

Strand: Reverse

Name: puuA [H]

Synonym: RC0671

Alternate gene names: 15892594

Gene position: 654138-653305 (Counterclockwise)

Preceding gene: 15892598

Following gene: 15892592

Centisome position: 51.56

GC content: 29.62

Gene sequence:

>834_bases
ATGAAAATATCCCATATTAATTATAATAGTTTTATTAATACTTTATCCGGTCGTTTACAAAAAAATAAACAGCTAGAAAA
AATAATAGCACAATTTCATCAAGACTATAACCTAATCCCTATTATTGGCGTAGAAATAGAGTTTTATTTAAGTCACAATA
TTGATATAGCTAAATTTGAAATACTTTCAAGAAAATACTTAGCTAGATTTAAAATTGCTAAAATAAAAAAAGAAAAAGGC
AATAACCAATTTGAAATAGATCTTCCCCCTTCTGTAAACTTAATACAATATATAAAAAATATATTAGAGGTTAAAACTAT
TTTAAAAAAAATGGCACAGCAGTTAAACGGTTATATAGATTTTTCGCCTAAGCCGTTTTTGGATGATTATGGTAATAGTA
TGCATTTTCATATTAATTTTAATTCCGAGTTCAATGATTATTATATAATTTTAGCAGCACAAGGGCTATGTCACTATATG
TTAGACACCCTACTCGCTTTTATGCCTACTACTCTAGATTATGCACGGATAAATAAAAAATTCATGGCTCCTACGCATAT
ATCGTACGGTGGAAATAATAGAAGCGTAGCCGTACGAACTCCAAATGCTTTTCCCAAACGTTTAGAACATCGCTTATCTT
CCCCCGAAACCGATCCATATATAGCAATTTTTACTATTTTAAAGTCAATATTGTTGGCTTTAAAATCTCCAAGTTCGCTC
CAAACAATAGAAAAAATTTACGGGAATGCTTTCGATCCACAATATAATTTAACTCCATTACCGACATCATCTCAGGCGAG
CTTTATGTTATTTAAACCGGATTTTTTTAAATAA

Upstream 100 bases:

>100_bases
TTCATTTATAGAGTTATTTTTTTTTATATATCTATGACTAGAATTATTGAATGTTTTATCAAAAATTTTATAATAAACTG
ATCCGTTTATTAATCCTTCC

Downstream 100 bases:

>100_bases
TTTGTATTTTGTTCACAAAACCTAAAAACTAAACAATAATTGTTTTTTACAACTAAAATAAAGATTGATATAAAATATTA
AATATAAAATTGCCGAAATT

Product: hypothetical protein

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKISHINYNSFINTLSGRLQKNKQLEKIIAQFHQDYNLIPIIGVEIEFYLSHNIDIAKFEILSRKYLARFKIAKIKKEKG
NNQFEIDLPPSVNLIQYIKNILEVKTILKKMAQQLNGYIDFSPKPFLDDYGNSMHFHINFNSEFNDYYIILAAQGLCHYM
LDTLLAFMPTTLDYARINKKFMAPTHISYGGNNRSVAVRTPNAFPKRLEHRLSSPETDPYIAIFTILKSILLALKSPSSL
QTIEKIYGNAFDPQYNLTPLPTSSQASFMLFKPDFFK

Sequences:

>Translated_277_residues
MKISHINYNSFINTLSGRLQKNKQLEKIIAQFHQDYNLIPIIGVEIEFYLSHNIDIAKFEILSRKYLARFKIAKIKKEKG
NNQFEIDLPPSVNLIQYIKNILEVKTILKKMAQQLNGYIDFSPKPFLDDYGNSMHFHINFNSEFNDYYIILAAQGLCHYM
LDTLLAFMPTTLDYARINKKFMAPTHISYGGNNRSVAVRTPNAFPKRLEHRLSSPETDPYIAIFTILKSILLALKSPSSL
QTIEKIYGNAFDPQYNLTPLPTSSQASFMLFKPDFFK
>Mature_277_residues
MKISHINYNSFINTLSGRLQKNKQLEKIIAQFHQDYNLIPIIGVEIEFYLSHNIDIAKFEILSRKYLARFKIAKIKKEKG
NNQFEIDLPPSVNLIQYIKNILEVKTILKKMAQQLNGYIDFSPKPFLDDYGNSMHFHINFNSEFNDYYIILAAQGLCHYM
LDTLLAFMPTTLDYARINKKFMAPTHISYGGNNRSVAVRTPNAFPKRLEHRLSSPETDPYIAIFTILKSILLALKSPSSL
QTIEKIYGNAFDPQYNLTPLPTSSQASFMLFKPDFFK

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081870, Length=277, Percent_Identity=24.9097472924188, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 32034; Mature: 32034

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISHINYNSFINTLSGRLQKNKQLEKIIAQFHQDYNLIPIIGVEIEFYLSHNIDIAKFE
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCHHHHH
ILSRKYLARFKIAKIKKEKGNNQFEIDLPPSVNLIQYIKNILEVKTILKKMAQQLNGYID
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEC
FSPKPFLDDYGNSMHFHINFNSEFNDYYIILAAQGLCHYMLDTLLAFMPTTLDYARINKK
CCCCCCHHHCCCEEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHCHH
FMAPTHISYGGNNRSVAVRTPNAFPKRLEHRLSSPETDPYIAIFTILKSILLALKSPSSL
HCCCCEEEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCHHH
QTIEKIYGNAFDPQYNLTPLPTSSQASFMLFKPDFFK
HHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MKISHINYNSFINTLSGRLQKNKQLEKIIAQFHQDYNLIPIIGVEIEFYLSHNIDIAKFE
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCHHHHH
ILSRKYLARFKIAKIKKEKGNNQFEIDLPPSVNLIQYIKNILEVKTILKKMAQQLNGYID
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEC
FSPKPFLDDYGNSMHFHINFNSEFNDYYIILAAQGLCHYMLDTLLAFMPTTLDYARINKK
CCCCCCHHHCCCEEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHCHH
FMAPTHISYGGNNRSVAVRTPNAFPKRLEHRLSSPETDPYIAIFTILKSILLALKSPSSL
HCCCCEEEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCHHH
QTIEKIYGNAFDPQYNLTPLPTSSQASFMLFKPDFFK
HHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]