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The map label for this gene is miaA

Identifier: 15892579

GI number: 15892579

Start: 636208

End: 637299

Strand: Reverse

Name: miaA

Synonym: RC0656

Alternate gene names: 15892579

Gene position: 637299-636208 (Counterclockwise)

Preceding gene: 15892580

Following gene: 15892560

Centisome position: 50.23

GC content: 31.04

Gene sequence:

>1092_bases
ATGCTGGCTTGCAATGACGATACAAGTTTGTATTTATTAGTTAAACAAGTGACAAAGAAAGAAATATACTCAAATGATTT
GGAGAATGGGAATGTAAAACGAGGAGCGAGTATGCAAAGCCTATATTTAATAGGTGATCCGAAGTGTTGCAGAAACAATT
CTTCAAAGCAGAAGAGTATAATTATATTATGCGGGCCGACTGCTAGCGGGAAGTCTTATTTAGGTCATGAACTGGCTAAA
GCTTATAACGGCGAAATCATTAATATCGACTCAATGCAGGTTTATAAGGAAATTCCTATTATTACCGCCTCCCCTCCGAA
AAGCTACAAAACTGAAATCTTGTATCACTTATATAACTTCTTATCAATGACAGAAGATTTCTCGGTAATAAAATATCTAA
AACTTGCGACAGAAAAAATAAAAGAAATAACCGACAGGGGTAAGCTACCTATATTAATAGGTGGAACGGGTTTATATATT
AATTCTTTAGTTTTTGGCTATAATAATATTCCTGATATATCAGAAGATTTACAAGAACAGGTAAGAAATTTACATGTTAA
AATAGGCAATATAGAATTATGGAGTAAATTAGAAAAGTTTGACCCGCTTGCTGCTTCTAAAATAAACCAAAATGATACTC
AGCGTTTAATTAGAGCTTATGAAGTATTCATGCAAACCGGTAAATCTATTTTTTCTTTTCAAACTTTGCCAAAAGAACAA
ATCTTATCTGATTTTAATTTCAAAATTATTTTCTTAAATCCTGAGCGAAAGTTTTTATATAAAACATGTGATGAGCGTTT
AGATAAGATATTTAAAGAAGGGGCAATTGACGAAATAGCTTTAATAAAAAAGCAATTTGCTCCTAAAGATTATACAAATT
TGAAAGCTGTAGGGATAAAAGAAATTTTAGCTTATTTAAACGGCAATCTCACTTTAGATGAGGCTTTAAATGCAGCTCAA
ATACGAACCCGTCAGTATGCTAAAAGACAAGTTACTTGGTTTAAAAATCAGATACAAGATAAAATAACATTGGAATATGC
TAACCAAGAAGAATTTACTCAAACTTTAAAAAACCCTTTTAAAATTATATAG

Upstream 100 bases:

>100_bases
GGATAGGTTTTTCCGTCATTGCGAGGAGTTGCGTTAGCAACGACGCGGCAATCTCATAAGATAATATAAAAAAACTCCTG
AGCTTGCCACGCCAGCATAA

Downstream 100 bases:

>100_bases
CTTATCTACGTATTGCTTCTTTCTTTTTATTATTGGAAATAGGAGTAATGGAAAGTGTATTTGTCTTAGGTCCTACAATA
TTAATCTTACTATTTAAATT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MLACNDDTSLYLLVKQVTKKEIYSNDLENGNVKRGASMQSLYLIGDPKCCRNNSSKQKSIIILCGPTASGKSYLGHELAK
AYNGEIINIDSMQVYKEIPIITASPPKSYKTEILYHLYNFLSMTEDFSVIKYLKLATEKIKEITDRGKLPILIGGTGLYI
NSLVFGYNNIPDISEDLQEQVRNLHVKIGNIELWSKLEKFDPLAASKINQNDTQRLIRAYEVFMQTGKSIFSFQTLPKEQ
ILSDFNFKIIFLNPERKFLYKTCDERLDKIFKEGAIDEIALIKKQFAPKDYTNLKAVGIKEILAYLNGNLTLDEALNAAQ
IRTRQYAKRQVTWFKNQIQDKITLEYANQEEFTQTLKNPFKII

Sequences:

>Translated_363_residues
MLACNDDTSLYLLVKQVTKKEIYSNDLENGNVKRGASMQSLYLIGDPKCCRNNSSKQKSIIILCGPTASGKSYLGHELAK
AYNGEIINIDSMQVYKEIPIITASPPKSYKTEILYHLYNFLSMTEDFSVIKYLKLATEKIKEITDRGKLPILIGGTGLYI
NSLVFGYNNIPDISEDLQEQVRNLHVKIGNIELWSKLEKFDPLAASKINQNDTQRLIRAYEVFMQTGKSIFSFQTLPKEQ
ILSDFNFKIIFLNPERKFLYKTCDERLDKIFKEGAIDEIALIKKQFAPKDYTNLKAVGIKEILAYLNGNLTLDEALNAAQ
IRTRQYAKRQVTWFKNQIQDKITLEYANQEEFTQTLKNPFKII
>Mature_363_residues
MLACNDDTSLYLLVKQVTKKEIYSNDLENGNVKRGASMQSLYLIGDPKCCRNNSSKQKSIIILCGPTASGKSYLGHELAK
AYNGEIINIDSMQVYKEIPIITASPPKSYKTEILYHLYNFLSMTEDFSVIKYLKLATEKIKEITDRGKLPILIGGTGLYI
NSLVFGYNNIPDISEDLQEQVRNLHVKIGNIELWSKLEKFDPLAASKINQNDTQRLIRAYEVFMQTGKSIFSFQTLPKEQ
ILSDFNFKIIFLNPERKFLYKTCDERLDKIFKEGAIDEIALIKKQFAPKDYTNLKAVGIKEILAYLNGNLTLDEALNAAQ
IRTRQYAKRQVTWFKNQIQDKITLEYANQEEFTQTLKNPFKII

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=312, Percent_Identity=27.5641025641026, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1790613, Length=278, Percent_Identity=30.2158273381295, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25144712, Length=300, Percent_Identity=30, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324848, Length=308, Percent_Identity=32.1428571428571, Blast_Score=159, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24649830, Length=269, Percent_Identity=27.5092936802974, Blast_Score=108, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_RICCN (Q92HW4)

Other databases:

- EMBL:   AE006914
- PIR:   H97781
- RefSeq:   NP_360293.1
- ProteinModelPortal:   Q92HW4
- SMR:   Q92HW4
- GeneID:   927735
- GenomeReviews:   AE006914_GR
- KEGG:   rco:RC0656
- NMPDR:   fig|272944.1.peg.656
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   RCON272944:RC0656-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 41637; Mature: 41637

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLACNDDTSLYLLVKQVTKKEIYSNDLENGNVKRGASMQSLYLIGDPKCCRNNSSKQKSI
CCEECCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHCCCCCCCCEE
IILCGPTASGKSYLGHELAKAYNGEIINIDSMQVYKEIPIITASPPKSYKTEILYHLYNF
EEEECCCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHCCEEECCCCCHHHHHHHHHHHHH
LSMTEDFSVIKYLKLATEKIKEITDRGKLPILIGGTGLYINSLVFGYNNIPDISEDLQEQ
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCHHHHHHHH
VRNLHVKIGNIELWSKLEKFDPLAASKINQNDTQRLIRAYEVFMQTGKSIFSFQTLPKEQ
HHHHEEEECCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCHHH
ILSDFNFKIIFLNPERKFLYKTCDERLDKIFKEGAIDEIALIKKQFAPKDYTNLKAVGIK
HHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHH
EILAYLNGNLTLDEALNAAQIRTRQYAKRQVTWFKNQIQDKITLEYANQEEFTQTLKNPF
HHHHHHCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCCC
KII
CCC
>Mature Secondary Structure
MLACNDDTSLYLLVKQVTKKEIYSNDLENGNVKRGASMQSLYLIGDPKCCRNNSSKQKSI
CCEECCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHCCCCCCCCEE
IILCGPTASGKSYLGHELAKAYNGEIINIDSMQVYKEIPIITASPPKSYKTEILYHLYNF
EEEECCCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHCCEEECCCCCHHHHHHHHHHHHH
LSMTEDFSVIKYLKLATEKIKEITDRGKLPILIGGTGLYINSLVFGYNNIPDISEDLQEQ
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCHHHHHHHH
VRNLHVKIGNIELWSKLEKFDPLAASKINQNDTQRLIRAYEVFMQTGKSIFSFQTLPKEQ
HHHHEEEECCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCHHH
ILSDFNFKIIFLNPERKFLYKTCDERLDKIFKEGAIDEIALIKKQFAPKDYTNLKAVGIK
HHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHH
EILAYLNGNLTLDEALNAAQIRTRQYAKRQVTWFKNQIQDKITLEYANQEEFTQTLKNPF
HHHHHHCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCCC
KII
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893