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The map label for this gene is topA

Identifier: 15892372

GI number: 15892372

Start: 442731

End: 445061

Strand: Reverse

Name: topA

Synonym: RC0449

Alternate gene names: 15892372

Gene position: 445061-442731 (Counterclockwise)

Preceding gene: 15892373

Following gene: 15892371

Centisome position: 35.08

GC content: 34.96

Gene sequence:

>2331_bases
ATGAAATTAGTAATAGTAGAATCGCCGGCAAAGGCAAAAACGATAAATAAATATTTAGGTGATGAGTTTAAGGTCATTGC
ATCATTCGGTCATATTAGAGATTTACCTTCTAAAAAAGGCTCGGTGTTACCTGATGAAAATTTTGCAATGAAATATGATA
TTTCCGATAAAGCCGGTAAATATGTAGATGCCATAGTTAAAGATGCTAAAAAAGCTGATGCAGTATATCTTGCAACCGAT
CCTGATCGTGAGGGTGAATCTATCTCATGGCATGTTGCAGAGGTAATAAAAGAAAAGAATCAAGTTAAATCCGATGATTT
TTTCAAAAGGGTAGCCTTTAACGAAATCACTAAAAAAGCAATTATTCATGCCGTTGAAAACCCTAGGAAACTTGACACTA
ACTTAGTAAATGCCCAACAAGCAAGAAGAGCTTTAGACTATTTAGTCGGCTTTACCCTTTCACCTCTTTTATGGCGTAAG
TTACCGGGATGTAAATCGGCAGGACGTGTGCAGTCCGTGGCTCTGCGATTAATATGTGAGCGAGAAGATGAAATAGAGCG
TTTTAAGTCAGAAGAATATTGGGATATTAGCCTTAAAATGCAAAATAGTAATAACGAACTATTTACTGCTAAATTGACTC
ACGTAAACGATCAGAAGTTAGAAAAATTCTCAATTATTAACGAAAAAAACGCCAAAGATTTAACCAAAAAATTAAAATCT
CACAAATTTCATGTTGATAAGATAGAAAAAAAACAACAAAAACGTCAACCACAACCTCCTTTTATTACTTCATCACTACA
ACAAGAAGCAGCAAGAAAATTAGGTTTTAGTGCTAAAAAGACTATGCAAATAGCACAAAAACTTTATGAGGGTGTTGATA
TAGGTAAGGAAACTATAGGGCTTATTACTTATATGAGAACCGACGGCGTTACATTATCAAATGATGCAATAGCAGATATA
CGTAAGTTAATCGATAAAAGTTACGGCGATAAATATTTACCGACTAGCCCTAGAATTTATAAATCCAAAGTAAAAAATGC
TCAAGAAGCTCATGAAGCAATAAGACCCACAAATATTACTTATACTCCTGATAGCTTAAAAGAAAAGCTAGACAAGGATT
ATTATAAGCTTTATGAGTTGATTTGGAAAAGAACTATAGCCTGCCAAATGGAAAATGTTATAATGGATTTGGTAGTTGCA
AATTTAGCTTCGGAAAATAAAGAATATTTGGCAAAAGCAAACGGATCGACTATAGCCTTTGACGGATTTTATAAGGTTTA
TCGTGAAAGTATGGACGATGAAGCTGAAGAAGAAAATAAAATGCTGCCGCCTTTAAAAGAACAAGAGCCGCTGAAAACTA
AAGCAGTTATTCCAAATCAACATTTTACCGAACCACCTCCAAGATATTCAGAAGCAAGTTTAGTGAAAAAACTTGAAGAG
CTTGGGATCGGTCGTCCTTCGACCTATGCTAGTATTTTATCGGTTTTACAAGATCGAAAATATGTTGCTCTTGAGAAAAA
ACGATTTATACCTGAAGAGCTTGGACGTTTGGTAACGGTATTTTTAGTCGGTTTCTTCAAAAAATACGTTGAATATGATT
TTACTGCAGGACTTGAAAACGAATTAGACGCAATAGCAGCCGGCAAACTTGAATGGAAAGCTGCTTTAAATAATTTTTGG
AGCGGTTTTAACCATAATATTGAATCAGTAAACGAGCAGAAAATAACCGAGATTATTAGCTATGTACAAAAAGCCCTTGA
TTATCACCTGTTCGGTGAGAATAAAGAATCTAAAATTTGTCCTTCATGTAACACGGGCGAGCTTAGCTTAAAGCTTGGTA
AGTTCGGAGCGTTTTTAGCATGTAGTAATTACCCTGAATGTACTTTTAGAAAATCTATTGTTAGTGGTAACGATAACAAC
GAGAATGAAGGCGAGCCTGCCGCTATGCCTAATGAGAATAAAGTTTTAGGTACGGATAAAGACGGAGTAGAAATATATCT
GAAAAAAGGACCTTACGGACCTTATATTCAACTTGGCAAACAAGAAGGCAAAGTAAAGCCGAAACGTAGCCCCGTGCCTG
CTAGCTTGAACCAAAATGATATTACACTTGATATAGCATTAAAACTTCTAAGCTTACCGCTAAAAATAGGTATTCATAAA
GATAGCGACGAGGAAATTATGATAGGATACGGTAAATTCGGTCCTTACATAAAATATATGGGTCAATTTATTTCGGTGCA
AAAAAAATATGATTTTCTAAATTTAAGCTTAGATGATGCGATGAAGCTAATTGAAGAGAATAAAGCGAAATTAGAGAAGA
AACAGGCGTAA

Upstream 100 bases:

>100_bases
ACCGCTTCCTATCATATATACGGTAATATTAGAATTAGAATTCGCCGGGACAGTAATGCGTCACGCCGGTAATAAAATAT
CATTAGTTTATAATAAGTAG

Downstream 100 bases:

>100_bases
ATGGTTTAGTGTCACCCCGTGACTTGATCACGGGGTCTCAGGACACAGGCTGTGATACAAGATCCCGTGGTCAAGCCACG
GGATGACAATTAATAAAATT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme

Number of amino acids: Translated: 776; Mature: 776

Protein sequence:

>776_residues
MKLVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDENFAMKYDISDKAGKYVDAIVKDAKKADAVYLATD
PDREGESISWHVAEVIKEKNQVKSDDFFKRVAFNEITKKAIIHAVENPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRK
LPGCKSAGRVQSVALRLICEREDEIERFKSEEYWDISLKMQNSNNELFTAKLTHVNDQKLEKFSIINEKNAKDLTKKLKS
HKFHVDKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGKETIGLITYMRTDGVTLSNDAIADI
RKLIDKSYGDKYLPTSPRIYKSKVKNAQEAHEAIRPTNITYTPDSLKEKLDKDYYKLYELIWKRTIACQMENVIMDLVVA
NLASENKEYLAKANGSTIAFDGFYKVYRESMDDEAEEENKMLPPLKEQEPLKTKAVIPNQHFTEPPPRYSEASLVKKLEE
LGIGRPSTYASILSVLQDRKYVALEKKRFIPEELGRLVTVFLVGFFKKYVEYDFTAGLENELDAIAAGKLEWKAALNNFW
SGFNHNIESVNEQKITEIISYVQKALDYHLFGENKESKICPSCNTGELSLKLGKFGAFLACSNYPECTFRKSIVSGNDNN
ENEGEPAAMPNENKVLGTDKDGVEIYLKKGPYGPYIQLGKQEGKVKPKRSPVPASLNQNDITLDIALKLLSLPLKIGIHK
DSDEEIMIGYGKFGPYIKYMGQFISVQKKYDFLNLSLDDAMKLIEENKAKLEKKQA

Sequences:

>Translated_776_residues
MKLVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDENFAMKYDISDKAGKYVDAIVKDAKKADAVYLATD
PDREGESISWHVAEVIKEKNQVKSDDFFKRVAFNEITKKAIIHAVENPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRK
LPGCKSAGRVQSVALRLICEREDEIERFKSEEYWDISLKMQNSNNELFTAKLTHVNDQKLEKFSIINEKNAKDLTKKLKS
HKFHVDKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGKETIGLITYMRTDGVTLSNDAIADI
RKLIDKSYGDKYLPTSPRIYKSKVKNAQEAHEAIRPTNITYTPDSLKEKLDKDYYKLYELIWKRTIACQMENVIMDLVVA
NLASENKEYLAKANGSTIAFDGFYKVYRESMDDEAEEENKMLPPLKEQEPLKTKAVIPNQHFTEPPPRYSEASLVKKLEE
LGIGRPSTYASILSVLQDRKYVALEKKRFIPEELGRLVTVFLVGFFKKYVEYDFTAGLENELDAIAAGKLEWKAALNNFW
SGFNHNIESVNEQKITEIISYVQKALDYHLFGENKESKICPSCNTGELSLKLGKFGAFLACSNYPECTFRKSIVSGNDNN
ENEGEPAAMPNENKVLGTDKDGVEIYLKKGPYGPYIQLGKQEGKVKPKRSPVPASLNQNDITLDIALKLLSLPLKIGIHK
DSDEEIMIGYGKFGPYIKYMGQFISVQKKYDFLNLSLDDAMKLIEENKAKLEKKQA
>Mature_776_residues
MKLVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDENFAMKYDISDKAGKYVDAIVKDAKKADAVYLATD
PDREGESISWHVAEVIKEKNQVKSDDFFKRVAFNEITKKAIIHAVENPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRK
LPGCKSAGRVQSVALRLICEREDEIERFKSEEYWDISLKMQNSNNELFTAKLTHVNDQKLEKFSIINEKNAKDLTKKLKS
HKFHVDKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGKETIGLITYMRTDGVTLSNDAIADI
RKLIDKSYGDKYLPTSPRIYKSKVKNAQEAHEAIRPTNITYTPDSLKEKLDKDYYKLYELIWKRTIACQMENVIMDLVVA
NLASENKEYLAKANGSTIAFDGFYKVYRESMDDEAEEENKMLPPLKEQEPLKTKAVIPNQHFTEPPPRYSEASLVKKLEE
LGIGRPSTYASILSVLQDRKYVALEKKRFIPEELGRLVTVFLVGFFKKYVEYDFTAGLENELDAIAAGKLEWKAALNNFW
SGFNHNIESVNEQKITEIISYVQKALDYHLFGENKESKICPSCNTGELSLKLGKFGAFLACSNYPECTFRKSIVSGNDNN
ENEGEPAAMPNENKVLGTDKDGVEIYLKKGPYGPYIQLGKQEGKVKPKRSPVPASLNQNDITLDIALKLLSLPLKIGIHK
DSDEEIMIGYGKFGPYIKYMGQFISVQKKYDFLNLSLDDAMKLIEENKAKLEKKQA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI10835218, Length=597, Percent_Identity=25.963149078727, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI4507635, Length=597, Percent_Identity=23.1155778894472, Blast_Score=109, Evalue=8e-24,
Organism=Escherichia coli, GI1787529, Length=786, Percent_Identity=37.6590330788804, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1788061, Length=574, Percent_Identity=23.8675958188153, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32563869, Length=561, Percent_Identity=24.5989304812834, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=544, Percent_Identity=26.8382352941176, Blast_Score=125, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=504, Percent_Identity=24.8015873015873, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24585251, Length=609, Percent_Identity=23.6453201970443, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24640096, Length=465, Percent_Identity=23.4408602150538, Blast_Score=97, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP1_RICCN (Q92IH1)

Other databases:

- EMBL:   AE006914
- PIR:   A97756
- RefSeq:   NP_360086.1
- ProteinModelPortal:   Q92IH1
- SMR:   Q92IH1
- GeneID:   927585
- GenomeReviews:   AE006914_GR
- KEGG:   rco:RC0449
- NMPDR:   fig|272944.1.peg.449
- OMA:   QMENVIM
- ProtClustDB:   PRK06599
- BioCyc:   RCON272944:RC0449-MONOMER
- BRENDA:   5.99.1.2
- GO:   GO:0005694
- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171
- Gene3D:   G3DSA:1.10.460.10
- Gene3D:   G3DSA:2.70.20.10
- PANTHER:   PTHR11390
- PRINTS:   PR00417
- SMART:   SM00437
- SMART:   SM00436
- SMART:   SM00493
- TIGRFAMs:   TIGR01051

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom; SSF56712 Topo_IA_core

EC number: =5.99.1.2

Molecular weight: Translated: 88146; Mature: 88146

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: ACT_SITE 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDENFAMKYDISDKAGK
CEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHH
YVDAIVKDAKKADAVYLATDPDREGESISWHVAEVIKEKNQVKSDDFFKRVAFNEITKKA
HHHHHHHHHHHCCEEEEECCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IIHAVENPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHC
REDEIERFKSEEYWDISLKMQNSNNELFTAKLTHVNDQKLEKFSIINEKNAKDLTKKLKS
CHHHHHHHCCCCEEEEEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHH
HKFHVDKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGKETIG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHH
LITYMRTDGVTLSNDAIADIRKLIDKSYGDKYLPTSPRIYKSKVKNAQEAHEAIRPTNIT
HHEEEECCCEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCE
YTPDSLKEKLDKDYYKLYELIWKRTIACQMENVIMDLVVANLASENKEYLAKANGSTIAF
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEE
DGFYKVYRESMDDEAEEENKMLPPLKEQEPLKTKAVIPNQHFTEPPPRYSEASLVKKLEE
HHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHH
LGIGRPSTYASILSVLQDRKYVALEKKRFIPEELGRLVTVFLVGFFKKYVEYDFTAGLEN
HCCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ELDAIAAGKLEWKAALNNFWSGFNHNIESVNEQKITEIISYVQKALDYHLFGENKESKIC
HHHHHHCCCHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PSCNTGELSLKLGKFGAFLACSNYPECTFRKSIVSGNDNNENEGEPAAMPNENKVLGTDK
CCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCC
DGVEIYLKKGPYGPYIQLGKQEGKVKPKRSPVPASLNQNDITLDIALKLLSLPLKIGIHK
CCEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHEEECC
DSDEEIMIGYGKFGPYIKYMGQFISVQKKYDFLNLSLDDAMKLIEENKAKLEKKQA
CCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDENFAMKYDISDKAGK
CEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHH
YVDAIVKDAKKADAVYLATDPDREGESISWHVAEVIKEKNQVKSDDFFKRVAFNEITKKA
HHHHHHHHHHHCCEEEEECCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IIHAVENPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHC
REDEIERFKSEEYWDISLKMQNSNNELFTAKLTHVNDQKLEKFSIINEKNAKDLTKKLKS
CHHHHHHHCCCCEEEEEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHH
HKFHVDKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGKETIG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHH
LITYMRTDGVTLSNDAIADIRKLIDKSYGDKYLPTSPRIYKSKVKNAQEAHEAIRPTNIT
HHEEEECCCEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCE
YTPDSLKEKLDKDYYKLYELIWKRTIACQMENVIMDLVVANLASENKEYLAKANGSTIAF
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEE
DGFYKVYRESMDDEAEEENKMLPPLKEQEPLKTKAVIPNQHFTEPPPRYSEASLVKKLEE
HHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHH
LGIGRPSTYASILSVLQDRKYVALEKKRFIPEELGRLVTVFLVGFFKKYVEYDFTAGLEN
HCCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ELDAIAAGKLEWKAALNNFWSGFNHNIESVNEQKITEIISYVQKALDYHLFGENKESKIC
HHHHHHCCCHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PSCNTGELSLKLGKFGAFLACSNYPECTFRKSIVSGNDNNENEGEPAAMPNENKVLGTDK
CCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCC
DGVEIYLKKGPYGPYIQLGKQEGKVKPKRSPVPASLNQNDITLDIALKLLSLPLKIGIHK
CCEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHEEECC
DSDEEIMIGYGKFGPYIKYMGQFISVQKKYDFLNLSLDDAMKLIEENKAKLEKKQA
CCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893