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The map label for this gene is gltX [H]

Identifier: 15892371

GI number: 15892371

Start: 441279

End: 442622

Strand: Reverse

Name: gltX [H]

Synonym: RC0448

Alternate gene names: 15892371

Gene position: 442622-441279 (Counterclockwise)

Preceding gene: 15892372

Following gene: 15892364

Centisome position: 34.89

GC content: 34.52

Gene sequence:

>1344_bases
ATGACAAAAGTTATAACACGATTTGCTCCGTCACCGACCGGTATGTTACATGTCGGGAATATCAGGGCAGCATTGCTTAA
CTGGCTATATGCAAAGAAGCATAACGGACAGTTTATTTTAAGATTTGACGATACGGATTTAGAGCGTAGTAAACAGGAAT
ATAAAGACGCTATCGAGGAAGATCTAAAATTTTTAAATATTCATTGGGATCAGACATTTAATCAATTAAGTCGTTTAAGT
AGATATGATGCAATCAAAAATCTATTACTAGACAAAAAAAGATTATATGCCTGCTATGAGACTCCTGAAGAGCTAGAATT
AAAGCGTAAATTTCAATTATCTAAAGGTTTACCACCGATTTATGATCGAGCTAGCTTAAATCTAACTGAAGAGCAAGCAA
AGAAATATATAGAACAAAGAAGAAAACCGCATTATAGATTTTTGGTAAATCATGAACCGATTAGCTGGCATGATATGATC
AAAGGCGAAGTTAAATATGACGGCAAGGCTTTAAGCGACCCAATAGTGATAAGAGCAGACGGTAGTATGACCTATATGTT
ATGCTCAGTTATTGATGATATTGATTATGATATTACTCATATTATTAGGGGTGAAGATCACGTTAGCAATACCGCCATTC
AAATGCAAATGTTTGAGGCTTTAAATACTACTCCTCCGACTTTTGGGCATTTAAGTTTAATAATCAATAAAGATGAGAAA
ATTTCTAAAAGAGTTGGAGGGTTTGAGATCGCAACTCTTAGAAAAGAAATCGGAATCGAAGCTATGGCAATCGCTAGCTT
TTTTAGTTTACTTGGTTCATCAGCACAAATCTTGCCTTATAAATCAATGGAGAAGCTAGCAAATCAATTTGAGATAAGTA
GTTTCTCTAAAAGTCCAACTATCTATCAGCCGGAAGATTTAGAAAGATTAAATCATAAATTACTTATAAGTTTAGATTTT
GATACAGTAAAAGAACGCCTTAAAGAAATAGATGCCGAGTATATTGATGAAAATTTTTGGTTATCGGTAAGCCCTAACTT
GCAAACATTACGTGATGTAAAAGATTGGTGGGAAATTTGCCATCAAACTCCGAATGTAGAAACTCTAAATTTAGATAAGG
AATATTTAAAGCAAGCAGCAGAATTATTACCGAAAGGTGAAATCACTAAAGATAGCTGGAGCATTTGGACTAAAGAAATA
ACAAATATAACAGGCAGGAAAGGTAAGGAGTTATTTTTACCTCTTCGCCTTGCTTTAACCGCCAGAGAATCAGGACCGGA
AATCGCAAGCGTTTTACCTTTGATTGATAGAGAAGAGATAATAAAGCGTTTAACTAGTGCGTGA

Upstream 100 bases:

>100_bases
GTGTCACCCCGTGACTTGATCACGGGGTCTCAGGACACAGGCTGTGATACAAGATCCCGTGGTCAAGCCACGGGATGACA
ATTAATAAAATTACTTATTT

Downstream 100 bases:

>100_bases
CTCTCTATGTCATTCCCGCGAAAGCGGGGATCCAGTAAAAATAATCTTATTGGTACAAAAATTGTATATTTGGTAAAATA
AACATATAAAAACAAGTTTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTKVITRFAPSPTGMLHVGNIRAALLNWLYAKKHNGQFILRFDDTDLERSKQEYKDAIEEDLKFLNIHWDQTFNQLSRLS
RYDAIKNLLLDKKRLYACYETPEELELKRKFQLSKGLPPIYDRASLNLTEEQAKKYIEQRRKPHYRFLVNHEPISWHDMI
KGEVKYDGKALSDPIVIRADGSMTYMLCSVIDDIDYDITHIIRGEDHVSNTAIQMQMFEALNTTPPTFGHLSLIINKDEK
ISKRVGGFEIATLRKEIGIEAMAIASFFSLLGSSAQILPYKSMEKLANQFEISSFSKSPTIYQPEDLERLNHKLLISLDF
DTVKERLKEIDAEYIDENFWLSVSPNLQTLRDVKDWWEICHQTPNVETLNLDKEYLKQAAELLPKGEITKDSWSIWTKEI
TNITGRKGKELFLPLRLALTARESGPEIASVLPLIDREEIIKRLTSA

Sequences:

>Translated_447_residues
MTKVITRFAPSPTGMLHVGNIRAALLNWLYAKKHNGQFILRFDDTDLERSKQEYKDAIEEDLKFLNIHWDQTFNQLSRLS
RYDAIKNLLLDKKRLYACYETPEELELKRKFQLSKGLPPIYDRASLNLTEEQAKKYIEQRRKPHYRFLVNHEPISWHDMI
KGEVKYDGKALSDPIVIRADGSMTYMLCSVIDDIDYDITHIIRGEDHVSNTAIQMQMFEALNTTPPTFGHLSLIINKDEK
ISKRVGGFEIATLRKEIGIEAMAIASFFSLLGSSAQILPYKSMEKLANQFEISSFSKSPTIYQPEDLERLNHKLLISLDF
DTVKERLKEIDAEYIDENFWLSVSPNLQTLRDVKDWWEICHQTPNVETLNLDKEYLKQAAELLPKGEITKDSWSIWTKEI
TNITGRKGKELFLPLRLALTARESGPEIASVLPLIDREEIIKRLTSA
>Mature_446_residues
TKVITRFAPSPTGMLHVGNIRAALLNWLYAKKHNGQFILRFDDTDLERSKQEYKDAIEEDLKFLNIHWDQTFNQLSRLSR
YDAIKNLLLDKKRLYACYETPEELELKRKFQLSKGLPPIYDRASLNLTEEQAKKYIEQRRKPHYRFLVNHEPISWHDMIK
GEVKYDGKALSDPIVIRADGSMTYMLCSVIDDIDYDITHIIRGEDHVSNTAIQMQMFEALNTTPPTFGHLSLIINKDEKI
SKRVGGFEIATLRKEIGIEAMAIASFFSLLGSSAQILPYKSMEKLANQFEISSFSKSPTIYQPEDLERLNHKLLISLDFD
TVKERLKEIDAEYIDENFWLSVSPNLQTLRDVKDWWEICHQTPNVETLNLDKEYLKQAAELLPKGEITKDSWSIWTKEIT
NITGRKGKELFLPLRLALTARESGPEIASVLPLIDREEIIKRLTSA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=27.1255060728745, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI62241042, Length=71, Percent_Identity=45.0704225352113, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788743, Length=468, Percent_Identity=31.6239316239316, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI226510925, Length=248, Percent_Identity=30.241935483871, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786895, Length=125, Percent_Identity=33.6, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=340, Percent_Identity=31.7647058823529, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17511007, Length=69, Percent_Identity=44.9275362318841, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17543332, Length=117, Percent_Identity=35.042735042735, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=484, Percent_Identity=28.9256198347107, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321192, Length=237, Percent_Identity=29.957805907173, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324742, Length=113, Percent_Identity=38.0530973451327, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24665321, Length=487, Percent_Identity=27.7207392197125, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24649466, Length=112, Percent_Identity=35.7142857142857, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 51783; Mature: 51652

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVITRFAPSPTGMLHVGNIRAALLNWLYAKKHNGQFILRFDDTDLERSKQEYKDAIEE
CCCCHHHCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
DLKFLNIHWDQTFNQLSRLSRYDAIKNLLLDKKRLYACYETPEELELKRKFQLSKGLPPI
HHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
YDRASLNLTEEQAKKYIEQRRKPHYRFLVNHEPISWHDMIKGEVKYDGKALSDPIVIRAD
CCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCEEEECCEECCCCEEEEEC
GSMTYMLCSVIDDIDYDITHIIRGEDHVSNTAIQMQMFEALNTTPPTFGHLSLIINKDEK
CCHHHHHHHHHHCCCCCHHEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHH
ISKRVGGFEIATLRKEIGIEAMAIASFFSLLGSSAQILPYKSMEKLANQFEISSFSKSPT
HHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
IYQPEDLERLNHKLLISLDFDTVKERLKEIDAEYIDENFWLSVSPNLQTLRDVKDWWEIC
CCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
HQTPNVETLNLDKEYLKQAAELLPKGEITKDSWSIWTKEITNITGRKGKELFLPLRLALT
CCCCCCEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEHEEEE
ARESGPEIASVLPLIDREEIIKRLTSA
ECCCCCHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TKVITRFAPSPTGMLHVGNIRAALLNWLYAKKHNGQFILRFDDTDLERSKQEYKDAIEE
CCCHHHCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
DLKFLNIHWDQTFNQLSRLSRYDAIKNLLLDKKRLYACYETPEELELKRKFQLSKGLPPI
HHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
YDRASLNLTEEQAKKYIEQRRKPHYRFLVNHEPISWHDMIKGEVKYDGKALSDPIVIRAD
CCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCEEEECCEECCCCEEEEEC
GSMTYMLCSVIDDIDYDITHIIRGEDHVSNTAIQMQMFEALNTTPPTFGHLSLIINKDEK
CCHHHHHHHHHHCCCCCHHEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHH
ISKRVGGFEIATLRKEIGIEAMAIASFFSLLGSSAQILPYKSMEKLANQFEISSFSKSPT
HHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
IYQPEDLERLNHKLLISLDFDTVKERLKEIDAEYIDENFWLSVSPNLQTLRDVKDWWEIC
CCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
HQTPNVETLNLDKEYLKQAAELLPKGEITKDSWSIWTKEITNITGRKGKELFLPLRLALT
CCCCCCEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEHEEEE
ARESGPEIASVLPLIDREEIIKRLTSA
ECCCCCHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA