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The map label for this gene is proP2 [H]

Identifier: 15892350

GI number: 15892350

Start: 422837

End: 424153

Strand: Reverse

Name: proP2 [H]

Synonym: RC0427

Alternate gene names: 15892350

Gene position: 424153-422837 (Counterclockwise)

Preceding gene: 15892351

Following gene: 15892348

Centisome position: 33.43

GC content: 30.75

Gene sequence:

>1317_bases
ATGTTAGGCTACGAAAAAGAACAAAGAAGTTTAAATACAGAACAAAAACAAGCAGTCGGATTACTTTCTATAGGTACATT
TCTAGAGTATTTTGACCTTATGCTTTATGTGCATATGGCGGTGCTTTTAAATAATTTATTTTTTCCGGAATATGATCCTT
TCACTTTTTCGTTGCTTACTGCCTTTTCTTTCTGTTCTACCTATTTGCTAAGACCTATAGGAGCATTAATATTTGGGTAT
ATAGGCGATCATTTTGGACGTAAGATTGTAGTTGTTTTAACTACCCTATTAATGGCTATTACTTGTGTGATTATAGGCAG
CATGCCAACATATGCACAGATAGGTATAACAGCCTCTTGGGTTCTTACTATATGTCGTATAATTCAAGGTATGTCAGCAA
CTGCAGAAGCACGAGGTGCAGAGCTGTACTTAACCGAAAATTCATCTCCTCCTATACAATATCCATTAGTAACAATTATA
ACCGTATTTTCTGCCGTAGGCACTACGGTAGCACTAGGTATTGCATCAATTTTTACTAATCAAAATATTTATCACCATGA
GTCAAGTTGGCGTATAGCCTTTTTCGTTGGTGCAACTATCGCTTTTGTAGGTACTATTGCAAGGACTAGTTTAAAAGAAG
CAGAAGCATTTTCAAATAAAAGAAACCAATTAAAACTAAGACTTCAAGAAAATAATATTATGCTTGAAGAAATTGACAAA
GATATTCTAGATCAAAAAGTTCCAAACACCACTTCTATTTGGTATTTCTTTATCCAGTGTGCTAGACCACCATGTTTTTA
TTTCGTATATATATATTGCGGTGATATATTAAAAAGAGAATGTGGATTTACTCCAAATCAAATTATAAATCAAAATTTTT
GGGTATCTATAATAGATTTACTCGGTATTATCGGTCTTGCATTTATTAGCTATAAAATCCATCCTTTAAAAATCCTAAAG
GTAAAATTATATCTATTTTTTACCTCTCTCATTTTTTTTCCTATAATGTTAAATTATAACCCTACTCCTTTATATATTTT
TATTTTTCAATGTTTAGCTGCATTATTCGTATTTGATCACGTACCAGCAGCTCCTATATTTTATAAATATTTCCCGGTTT
TTAAGAGATTTACATATACTAGTATGCTAAGTGCAATAGCAAAATTATTTACTTATATTATAACGTCATTTGGTTTAGTT
TATATTACCAATTATCTAGGATATTGGGGATTATTTTTAATTTTCATTCCAGTAGGTATAACTTTTTTTATGGGAGTAAC
TTATTTTGAGAAAATGGAAACGAATCGAAATTTATAA

Upstream 100 bases:

>100_bases
CTAAATATTTACAATATTTATTAAAAATTCTTAATCCTTTATTGACTTTGAATCAAAAGCAGTATAGCTTATTTTGTAAT
CTAATTAATATACAAAGGTT

Downstream 100 bases:

>100_bases
TTTTATTAAAAGTAGAATTTATATTAAATTTTAAATTATTTTTATAACATTCAGCATATATAAGCTCTGAAACTGAAGGT
ATTTCAAGTATTTCACTTAA

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MLGYEKEQRSLNTEQKQAVGLLSIGTFLEYFDLMLYVHMAVLLNNLFFPEYDPFTFSLLTAFSFCSTYLLRPIGALIFGY
IGDHFGRKIVVVLTTLLMAITCVIIGSMPTYAQIGITASWVLTICRIIQGMSATAEARGAELYLTENSSPPIQYPLVTII
TVFSAVGTTVALGIASIFTNQNIYHHESSWRIAFFVGATIAFVGTIARTSLKEAEAFSNKRNQLKLRLQENNIMLEEIDK
DILDQKVPNTTSIWYFFIQCARPPCFYFVYIYCGDILKRECGFTPNQIINQNFWVSIIDLLGIIGLAFISYKIHPLKILK
VKLYLFFTSLIFFPIMLNYNPTPLYIFIFQCLAALFVFDHVPAAPIFYKYFPVFKRFTYTSMLSAIAKLFTYIITSFGLV
YITNYLGYWGLFLIFIPVGITFFMGVTYFEKMETNRNL

Sequences:

>Translated_438_residues
MLGYEKEQRSLNTEQKQAVGLLSIGTFLEYFDLMLYVHMAVLLNNLFFPEYDPFTFSLLTAFSFCSTYLLRPIGALIFGY
IGDHFGRKIVVVLTTLLMAITCVIIGSMPTYAQIGITASWVLTICRIIQGMSATAEARGAELYLTENSSPPIQYPLVTII
TVFSAVGTTVALGIASIFTNQNIYHHESSWRIAFFVGATIAFVGTIARTSLKEAEAFSNKRNQLKLRLQENNIMLEEIDK
DILDQKVPNTTSIWYFFIQCARPPCFYFVYIYCGDILKRECGFTPNQIINQNFWVSIIDLLGIIGLAFISYKIHPLKILK
VKLYLFFTSLIFFPIMLNYNPTPLYIFIFQCLAALFVFDHVPAAPIFYKYFPVFKRFTYTSMLSAIAKLFTYIITSFGLV
YITNYLGYWGLFLIFIPVGITFFMGVTYFEKMETNRNL
>Mature_438_residues
MLGYEKEQRSLNTEQKQAVGLLSIGTFLEYFDLMLYVHMAVLLNNLFFPEYDPFTFSLLTAFSFCSTYLLRPIGALIFGY
IGDHFGRKIVVVLTTLLMAITCVIIGSMPTYAQIGITASWVLTICRIIQGMSATAEARGAELYLTENSSPPIQYPLVTII
TVFSAVGTTVALGIASIFTNQNIYHHESSWRIAFFVGATIAFVGTIARTSLKEAEAFSNKRNQLKLRLQENNIMLEEIDK
DILDQKVPNTTSIWYFFIQCARPPCFYFVYIYCGDILKRECGFTPNQIINQNFWVSIIDLLGIIGLAFISYKIHPLKILK
VKLYLFFTSLIFFPIMLNYNPTPLYIFIFQCLAALFVFDHVPAAPIFYKYFPVFKRFTYTSMLSAIAKLFTYIITSFGLV
YITNYLGYWGLFLIFIPVGITFFMGVTYFEKMETNRNL

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=225, Percent_Identity=30.2222222222222, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789941, Length=428, Percent_Identity=26.6355140186916, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788292, Length=221, Percent_Identity=27.1493212669683, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788942, Length=193, Percent_Identity=29.5336787564767, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 50094; Mature: 50094

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGYEKEQRSLNTEQKQAVGLLSIGTFLEYFDLMLYVHMAVLLNNLFFPEYDPFTFSLLT
CCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
AFSFCSTYLLRPIGALIFGYIGDHFGRKIVVVLTTLLMAITCVIIGSMPTYAQIGITASW
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHH
VLTICRIIQGMSATAEARGAELYLTENSSPPIQYPLVTIITVFSAVGTTVALGIASIFTN
HHHHHHHHCCCCCCHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
QNIYHHESSWRIAFFVGATIAFVGTIARTSLKEAEAFSNKRNQLKLRLQENNIMLEEIDK
CCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
DILDQKVPNTTSIWYFFIQCARPPCFYFVYIYCGDILKRECGFTPNQIINQNFWVSIIDL
HHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHH
LGIIGLAFISYKIHPLKILKVKLYLFFTSLIFFPIMLNYNPTPLYIFIFQCLAALFVFDH
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
VPAAPIFYKYFPVFKRFTYTSMLSAIAKLFTYIITSFGLVYITNYLGYWGLFLIFIPVGI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFFMGVTYFEKMETNRNL
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLGYEKEQRSLNTEQKQAVGLLSIGTFLEYFDLMLYVHMAVLLNNLFFPEYDPFTFSLLT
CCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
AFSFCSTYLLRPIGALIFGYIGDHFGRKIVVVLTTLLMAITCVIIGSMPTYAQIGITASW
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHH
VLTICRIIQGMSATAEARGAELYLTENSSPPIQYPLVTIITVFSAVGTTVALGIASIFTN
HHHHHHHHCCCCCCHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
QNIYHHESSWRIAFFVGATIAFVGTIARTSLKEAEAFSNKRNQLKLRLQENNIMLEEIDK
CCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
DILDQKVPNTTSIWYFFIQCARPPCFYFVYIYCGDILKRECGFTPNQIINQNFWVSIIDL
HHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHH
LGIIGLAFISYKIHPLKILKVKLYLFFTSLIFFPIMLNYNPTPLYIFIFQCLAALFVFDH
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
VPAAPIFYKYFPVFKRFTYTSMLSAIAKLFTYIITSFGLVYITNYLGYWGLFLIFIPVGI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFFMGVTYFEKMETNRNL
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]