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The map label for this gene is queA [H]

Identifier: 15892206

GI number: 15892206

Start: 287340

End: 288437

Strand: Reverse

Name: queA [H]

Synonym: RC0283

Alternate gene names: 15892206

Gene position: 288437-287340 (Counterclockwise)

Preceding gene: 15892209

Following gene: 15892205

Centisome position: 22.73

GC content: 34.7

Gene sequence:

>1098_bases
ATGAAACTATCTGATTTTGACTTTGATTTACCAAGCGAACTGATCGCACAATATCCATCTAGTGAACGTGATAACTCAGA
TTTACTAATTGCTGTTACGCCGCCTATTAAAACCAAATTCTACAACATAATAGATTACTTAAAAGAAGGGGACTTATTAG
TCTTTAATAATAGTAAGGTAATAAAAGCAAAGTTAAATTTGGGGAAAAATATTACAATAAATTTGAATCAAAAATTATCA
GATGATAATTGGTCGGCTTTTGCTAAACCAGCTCGAAAGCTTCATGTAAATGATGAATTTTATTTTGATAATCATAAGGT
AATTATTACCGAAAAACTTGCAATGGGTGAGATTAAAGTAAAATTTGAGCTTAATGATATTTCTGTATTCGAGTTCTTAA
ATAAATACGGTGAGATGCCGCTACCGGTTTATATTAGGCGTTCACACTCATTATGTCATCCCGTGGCTACGACCACGGGA
TCCAAAACATATTTAAATAATGACTGGATACCGTGGTCAAACCACGGGATGACAAACACTCAAAACGACAACGACCGCTA
TCAAACTGTCTATAGCCAAATAGAAGGTTCAGTTGCAGCACCGACAGCAGGCTTGCATTTTACAAAGGATATACTTGATA
AGCTTAAGGCAGAAGGCATACAAGCCACATTTTTAACATTACATGTGGGAGCTGGAACTTTTTTACCTGTAAAAACCGAA
AATATTCATGAACATAAAATGCATACCGAATATTGCTCTATTACTCCTGACACTGCTGAAATTATCAATAAAGCAAAGCA
AGAAGGGAAACGTATTATCGCAGTCGGCACTACAACACTTAGAACACTTGAGAGTTCTTGCAATAACGGCATCGTAAAAG
CAGGTAGCTTTAAAACCGATATTTTCATAACCCCAGGTTTTAAATTTCAAACAGCCGATATGCTACTTACTAATTTCCAT
TTTCCTAAATCTACTTTATTTATGCTTATATGTGCTTTTGCCGGATTTAAAGAGATGCATGAATTATATAAATATGCTAT
AAAAGAAGCAATGCGTTTTTTTAGCTACGGCGATGCAACGCTTTTATGTAGGAAATGA

Upstream 100 bases:

>100_bases
AATAGTTTATTTATTAGTAAGCAATCAATAAACTAAAATATTATATTAAATATTGTCATATGCTGTTATAATCACTTTTT
TAGCTACTAATATTTAAACA

Downstream 100 bases:

>100_bases
TAATCACAACCGTCATTGCGAGCAACTAAAAGGAGCGTGCCAATCTAGCAAAAATAACAAAAAATGCTAAAGGTTAGCAT
TTTTCACTGGATCCCGTGGT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKLSDFDFDLPSELIAQYPSSERDNSDLLIAVTPPIKTKFYNIIDYLKEGDLLVFNNSKVIKAKLNLGKNITINLNQKLS
DDNWSAFAKPARKLHVNDEFYFDNHKVIITEKLAMGEIKVKFELNDISVFEFLNKYGEMPLPVYIRRSHSLCHPVATTTG
SKTYLNNDWIPWSNHGMTNTQNDNDRYQTVYSQIEGSVAAPTAGLHFTKDILDKLKAEGIQATFLTLHVGAGTFLPVKTE
NIHEHKMHTEYCSITPDTAEIINKAKQEGKRIIAVGTTTLRTLESSCNNGIVKAGSFKTDIFITPGFKFQTADMLLTNFH
FPKSTLFMLICAFAGFKEMHELYKYAIKEAMRFFSYGDATLLCRK

Sequences:

>Translated_365_residues
MKLSDFDFDLPSELIAQYPSSERDNSDLLIAVTPPIKTKFYNIIDYLKEGDLLVFNNSKVIKAKLNLGKNITINLNQKLS
DDNWSAFAKPARKLHVNDEFYFDNHKVIITEKLAMGEIKVKFELNDISVFEFLNKYGEMPLPVYIRRSHSLCHPVATTTG
SKTYLNNDWIPWSNHGMTNTQNDNDRYQTVYSQIEGSVAAPTAGLHFTKDILDKLKAEGIQATFLTLHVGAGTFLPVKTE
NIHEHKMHTEYCSITPDTAEIINKAKQEGKRIIAVGTTTLRTLESSCNNGIVKAGSFKTDIFITPGFKFQTADMLLTNFH
FPKSTLFMLICAFAGFKEMHELYKYAIKEAMRFFSYGDATLLCRK
>Mature_365_residues
MKLSDFDFDLPSELIAQYPSSERDNSDLLIAVTPPIKTKFYNIIDYLKEGDLLVFNNSKVIKAKLNLGKNITINLNQKLS
DDNWSAFAKPARKLHVNDEFYFDNHKVIITEKLAMGEIKVKFELNDISVFEFLNKYGEMPLPVYIRRSHSLCHPVATTTG
SKTYLNNDWIPWSNHGMTNTQNDNDRYQTVYSQIEGSVAAPTAGLHFTKDILDKLKAEGIQATFLTLHVGAGTFLPVKTE
NIHEHKMHTEYCSITPDTAEIINKAKQEGKRIIAVGTTTLRTLESSCNNGIVKAGSFKTDIFITPGFKFQTADMLLTNFH
FPKSTLFMLICAFAGFKEMHELYKYAIKEAMRFFSYGDATLLCRK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=374, Percent_Identity=39.0374331550802, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 41428; Mature: 41428

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSDFDFDLPSELIAQYPSSERDNSDLLIAVTPPIKTKFYNIIDYLKEGDLLVFNNSKV
CCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCEE
IKAKLNLGKNITINLNQKLSDDNWSAFAKPARKLHVNDEFYFDNHKVIITEKLAMGEIKV
EEEEECCCCEEEEEECCCCCCCCCHHHHCCCCEEECCCCEEECCCEEEEEEEEEECEEEE
KFELNDISVFEFLNKYGEMPLPVYIRRSHSLCHPVATTTGSKTYLNNDWIPWSNHGMTNT
EEEECCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHCCCCCCEEECCCCCCCCCCCCCCC
QNDNDRYQTVYSQIEGSVAAPTAGLHFTKDILDKLKAEGIQATFLTLHVGAGTFLPVKTE
CCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEECC
NIHEHKMHTEYCSITPDTAEIINKAKQEGKRIIAVGTTTLRTLESSCNNGIVKAGSFKTD
CCHHHHHCCHHCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCEEECCCCEEE
IFITPGFKFQTADMLLTNFHFPKSTLFMLICAFAGFKEMHELYKYAIKEAMRFFSYGDAT
EEECCCCCEEHHHHHEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEE
LLCRK
EEEEC
>Mature Secondary Structure
MKLSDFDFDLPSELIAQYPSSERDNSDLLIAVTPPIKTKFYNIIDYLKEGDLLVFNNSKV
CCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCEE
IKAKLNLGKNITINLNQKLSDDNWSAFAKPARKLHVNDEFYFDNHKVIITEKLAMGEIKV
EEEEECCCCEEEEEECCCCCCCCCHHHHCCCCEEECCCCEEECCCEEEEEEEEEECEEEE
KFELNDISVFEFLNKYGEMPLPVYIRRSHSLCHPVATTTGSKTYLNNDWIPWSNHGMTNT
EEEECCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHCCCCCCEEECCCCCCCCCCCCCCC
QNDNDRYQTVYSQIEGSVAAPTAGLHFTKDILDKLKAEGIQATFLTLHVGAGTFLPVKTE
CCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEECC
NIHEHKMHTEYCSITPDTAEIINKAKQEGKRIIAVGTTTLRTLESSCNNGIVKAGSFKTD
CCHHHHHCCHHCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCEEECCCCEEE
IFITPGFKFQTADMLLTNFHFPKSTLFMLICAFAGFKEMHELYKYAIKEAMRFFSYGDAT
EEECCCCCEEHHHHHEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEE
LLCRK
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA