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The map label for this gene is gatB [H]

Identifier: 15892116

GI number: 15892116

Start: 200348

End: 201799

Strand: Reverse

Name: gatB [H]

Synonym: RC0193

Alternate gene names: 15892116

Gene position: 201799-200348 (Counterclockwise)

Preceding gene: 15892117

Following gene: 15892115

Centisome position: 15.91

GC content: 35.81

Gene sequence:

>1452_bases
ATGGCATATATTGAAGGTAATACGGGGAAATGGGAATATGTAATAGGGCTTGAAATCCATGCTCAAATTTCCTCAAAATC
TAAGCTTTTTTCAGGAAGTAGTACTATTTTTGCAGCAAATCCAAATTCGCAGGTTTCCTATGTCGATGCAGCAATGCCCG
GGATGTTACCGGTATTAAATAAGCATTGTGTACACCAAGCAATTAAAACAGGGCTTGGGCTTAAAGCTAAAATAAATAAA
TATTCGGTATTTGACCGTAAAAATTATTTTTATGCCGATCTGCCGCAAGGTTACCAAATTTCGCAATTTTACTATCCTAT
AGTACAAAACGGCACTATGGAAATACCGACTAGTACCGGCGATCTTAAAACTATTCGTATTAACCGTTTGCATTTAGAGC
AAGATGCGGGTAAATCTATGCATGATCAATCACCACATTACAGCTTTATCGATTTGAATCGTGCCGGTATTGGACTTATG
GAAATTGTGACCGAACCTGATATATCATCACCGGAAGAAGCAGCTGAATTTGTAAAAAAGCTAAGAAATTTGTTACGTTA
TATTGGCAGCTGTGACGGTGATATGGAAAAAGGCTCGATGCGTTGCGATGCTAATATTTCAGTAAGACGTAGCGGCGAAC
CACTCGGCACAAGATGCGAAATTAAAAATATTAATTCAATTCGTAACATCATTAAAGCAATAGAATTTGAAGCTAAAAGA
CAAGTTGACTTGCTTGAAAGCGGTGAAAAAATAATTCAAGAAACACGCCTATTTAATGCCGATAGCGGCGAAACAAGAAC
TATGCGTTTAAAAGAAGAAGCACTTGATTATAGATATTTTCCTGATCCCGATTTATTACCGCTTGTTATTTCTGATGAGT
TAATAAATGAGCTAAAAGCAAATTTACCTGAGCTACCTGATCAAAAGATTGAAAAATATACGAAGGAATTTAGCTTAAGT
AAATATGATGCCGAAGTAATAGTAGCCGATGAATCGGTTGCTGAGTATTTTGAGAAAGCAGCAAATGAATGTAACCCTAA
AATGCTTACTAATTGGCTAACTAGTGAATTATTTGGCCAATTAAATAAAGCATCGATAGGAATCAATGAATGCAAAATTA
CCCCTAGTAACTTTGCAAAACTGGTAAAATTAATAGAAAATGATACTATTTCCGGTAAAATTGCAAAAACCGTTTTTGAA
ATTATGTTTGAAACCGGTAAAGCACCTGATAAAATAGTAGAAGAAAAAGGACTTGTACAAGTTTCGGATAATAACGTGCT
AAACACGGTAATTGACGAGGTAATAGCTGAAAATCCTGAATCAGTAGAGGGTTATAGAAGTGGTAAGGATAAATTATTCG
GTTTCTTTGTAGGACAGGTAATGAAAAAAACCGGCGGTAAGGCTAATCCGACGCTTGTGAATCAGCTTTTAAAGGAGAAA
TTGAGTTCTTGA

Upstream 100 bases:

>100_bases
TTATAGGTAAACAATTAGACGAATATAATGTTTTGAAAGTAGCATCGACAATTGAGTCAGGTGTAAAACATATTAAATTT
GAACCAAAGGGTTTTTAATT

Downstream 100 bases:

>100_bases
TGCTATTCCTTTGAAGATATTGCCCGAGTGGATAGATTTTTCTGTCATTGCGAGCAGCCATAGACTTTGTTGCATGGCTC
GGTTTCATCGTCATTGCGAG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MAYIEGNTGKWEYVIGLEIHAQISSKSKLFSGSSTIFAANPNSQVSYVDAAMPGMLPVLNKHCVHQAIKTGLGLKAKINK
YSVFDRKNYFYADLPQGYQISQFYYPIVQNGTMEIPTSTGDLKTIRINRLHLEQDAGKSMHDQSPHYSFIDLNRAGIGLM
EIVTEPDISSPEEAAEFVKKLRNLLRYIGSCDGDMEKGSMRCDANISVRRSGEPLGTRCEIKNINSIRNIIKAIEFEAKR
QVDLLESGEKIIQETRLFNADSGETRTMRLKEEALDYRYFPDPDLLPLVISDELINELKANLPELPDQKIEKYTKEFSLS
KYDAEVIVADESVAEYFEKAANECNPKMLTNWLTSELFGQLNKASIGINECKITPSNFAKLVKLIENDTISGKIAKTVFE
IMFETGKAPDKIVEEKGLVQVSDNNVLNTVIDEVIAENPESVEGYRSGKDKLFGFFVGQVMKKTGGKANPTLVNQLLKEK
LSS

Sequences:

>Translated_483_residues
MAYIEGNTGKWEYVIGLEIHAQISSKSKLFSGSSTIFAANPNSQVSYVDAAMPGMLPVLNKHCVHQAIKTGLGLKAKINK
YSVFDRKNYFYADLPQGYQISQFYYPIVQNGTMEIPTSTGDLKTIRINRLHLEQDAGKSMHDQSPHYSFIDLNRAGIGLM
EIVTEPDISSPEEAAEFVKKLRNLLRYIGSCDGDMEKGSMRCDANISVRRSGEPLGTRCEIKNINSIRNIIKAIEFEAKR
QVDLLESGEKIIQETRLFNADSGETRTMRLKEEALDYRYFPDPDLLPLVISDELINELKANLPELPDQKIEKYTKEFSLS
KYDAEVIVADESVAEYFEKAANECNPKMLTNWLTSELFGQLNKASIGINECKITPSNFAKLVKLIENDTISGKIAKTVFE
IMFETGKAPDKIVEEKGLVQVSDNNVLNTVIDEVIAENPESVEGYRSGKDKLFGFFVGQVMKKTGGKANPTLVNQLLKEK
LSS
>Mature_482_residues
AYIEGNTGKWEYVIGLEIHAQISSKSKLFSGSSTIFAANPNSQVSYVDAAMPGMLPVLNKHCVHQAIKTGLGLKAKINKY
SVFDRKNYFYADLPQGYQISQFYYPIVQNGTMEIPTSTGDLKTIRINRLHLEQDAGKSMHDQSPHYSFIDLNRAGIGLME
IVTEPDISSPEEAAEFVKKLRNLLRYIGSCDGDMEKGSMRCDANISVRRSGEPLGTRCEIKNINSIRNIIKAIEFEAKRQ
VDLLESGEKIIQETRLFNADSGETRTMRLKEEALDYRYFPDPDLLPLVISDELINELKANLPELPDQKIEKYTKEFSLSK
YDAEVIVADESVAEYFEKAANECNPKMLTNWLTSELFGQLNKASIGINECKITPSNFAKLVKLIENDTISGKIAKTVFEI
MFETGKAPDKIVEEKGLVQVSDNNVLNTVIDEVIAENPESVEGYRSGKDKLFGFFVGQVMKKTGGKANPTLVNQLLKEKL
SS

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=42.6262626262626, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI115534094, Length=303, Percent_Identity=44.2244224422442, Blast_Score=227, Evalue=9e-60,
Organism=Saccharomyces cerevisiae, GI6319391, Length=527, Percent_Identity=33.7760910815939, Blast_Score=265, Evalue=1e-71,
Organism=Drosophila melanogaster, GI45550808, Length=490, Percent_Identity=38.1632653061225, Blast_Score=345, Evalue=4e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54076; Mature: 53945

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYIEGNTGKWEYVIGLEIHAQISSKSKLFSGSSTIFAANPNSQVSYVDAAMPGMLPVLN
CEEEECCCCCEEEEEEEEEEEEECCCCCEECCCCEEEEECCCCCEEEEHHHCCCHHHHHH
KHCVHQAIKTGLGLKAKINKYSVFDRKNYFYADLPQGYQISQFYYPIVQNGTMEIPTSTG
HHHHHHHHHHCCCCEEECCCEEEEECCCCEEEECCCCCEEHHEEEHHHCCCEEECCCCCC
DLKTIRINRLHLEQDAGKSMHDQSPHYSFIDLNRAGIGLMEIVTEPDISSPEEAAEFVKK
CEEEEEEEEEEECHHCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHH
LRNLLRYIGSCDGDMEKGSMRCDANISVRRSGEPLGTRCEIKNINSIRNIIKAIEFEAKR
HHHHHHHHHCCCCCCCCCCEEECCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHH
QVDLLESGEKIIQETRLFNADSGETRTMRLKEEALDYRYFPDPDLLPLVISDELINELKA
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCCCCCEEEEECHHHHHHHHH
NLPELPDQKIEKYTKEFSLSKYDAEVIVADESVAEYFEKAANECNPKMLTNWLTSELFGQ
CCCCCCHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
LNKASIGINECKITPSNFAKLVKLIENDTISGKIAKTVFEIMFETGKAPDKIVEEKGLVQ
CCCCCCCCCCEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCEE
VSDNNVLNTVIDEVIAENPESVEGYRSGKDKLFGFFVGQVMKKTGGKANPTLVNQLLKEK
ECCCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LSS
HCC
>Mature Secondary Structure 
AYIEGNTGKWEYVIGLEIHAQISSKSKLFSGSSTIFAANPNSQVSYVDAAMPGMLPVLN
EEEECCCCCEEEEEEEEEEEEECCCCCEECCCCEEEEECCCCCEEEEHHHCCCHHHHHH
KHCVHQAIKTGLGLKAKINKYSVFDRKNYFYADLPQGYQISQFYYPIVQNGTMEIPTSTG
HHHHHHHHHHCCCCEEECCCEEEEECCCCEEEECCCCCEEHHEEEHHHCCCEEECCCCCC
DLKTIRINRLHLEQDAGKSMHDQSPHYSFIDLNRAGIGLMEIVTEPDISSPEEAAEFVKK
CEEEEEEEEEEECHHCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHH
LRNLLRYIGSCDGDMEKGSMRCDANISVRRSGEPLGTRCEIKNINSIRNIIKAIEFEAKR
HHHHHHHHHCCCCCCCCCCEEECCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHH
QVDLLESGEKIIQETRLFNADSGETRTMRLKEEALDYRYFPDPDLLPLVISDELINELKA
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCCCCCEEEEECHHHHHHHHH
NLPELPDQKIEKYTKEFSLSKYDAEVIVADESVAEYFEKAANECNPKMLTNWLTSELFGQ
CCCCCCHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
LNKASIGINECKITPSNFAKLVKLIENDTISGKIAKTVFEIMFETGKAPDKIVEEKGLVQ
CCCCCCCCCCEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCEE
VSDNNVLNTVIDEVIAENPESVEGYRSGKDKLFGFFVGQVMKKTGGKANPTLVNQLLKEK
ECCCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LSS
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA