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The map label for this gene is yqiX [H]

Identifier: 15892115

GI number: 15892115

Start: 199366

End: 200091

Strand: Reverse

Name: yqiX [H]

Synonym: RC0192

Alternate gene names: 15892115

Gene position: 200091-199366 (Counterclockwise)

Preceding gene: 15892116

Following gene: 15892114

Centisome position: 15.77

GC content: 31.54

Gene sequence:

>726_bases
ATGAAATTTCTGAAAATATTTTTACTATCTTGCTTACTAGTTTCTTGTGAAAAAAAAGAAACGGAAAAAACTTTAATAGT
CGGTACTGCCGCAGATAATCCTCCTTATGAATTTATCCAAGACGGAGAGATTGTTGGGATTGATATTGATATTATCAAAG
CAATAAGCGAACGTCTAAATAAAAAAGTTATCATAAAAAACTTCGACTTTAACGGTTTACTTGCAGCTTTGGTTAGTGAA
AATATTGATGTTGCGGTGGCGGAGCTTTCCGTAACACCGGAGCGTGCTGAATATATCAGCTTTTCCGATAATTATGCTAC
TGCAAGATTTGCCATAATATATAGAACAGGTGATAATATACAAAATAGCAAAGATCTAGAAAATAAAATAGTAGGGGTAC
AGCTCGGTAGTGTTTTAGAAAAAAAAGCACACGATCTATCACAGACGATAAATATTAAAGTTCATTCTTTAGCAAATCAT
TTAATGTTGGTTGAAGAATTAAAAGCCAGAGTCATAGATGCTATTATTTCTGAGGAATTTCAAGGCAAAAAATTAAAAGA
AAATAATTCAAATTTAGAAAGCAGTACTTTAGAAGAATTTTCATCCGATTTTGCTATAGGCATGTCTAAAAACTCAGGAC
TAATTAATGAAATTAATGAGGCAATCGATTCATTAAAAAAAGACGGAACCATTAACAAAATTATGAAAAAATGGCTGGGA
CAGTAA

Upstream 100 bases:

>100_bases
CTACGTAATTTTCCTCGCAATGACGATTTGATATCCGTACAACAAGGGCAATGCCTCGTAGGATGACACAGAATATACTC
AAACTTAAAACATTATTTTT

Downstream 100 bases:

>100_bases
CAGAAAGTCAATTTAAAGATGCTATGAGCCGATTCCCTCAGGGAGTGACTATTATAACAACAAATTGCAATAACGAACTT
TTTGGCTTTACTGCTAGTTC

Product: amino acid ABC transporter substrate binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MKFLKIFLLSCLLVSCEKKETEKTLIVGTAADNPPYEFIQDGEIVGIDIDIIKAISERLNKKVIIKNFDFNGLLAALVSE
NIDVAVAELSVTPERAEYISFSDNYATARFAIIYRTGDNIQNSKDLENKIVGVQLGSVLEKKAHDLSQTINIKVHSLANH
LMLVEELKARVIDAIISEEFQGKKLKENNSNLESSTLEEFSSDFAIGMSKNSGLINEINEAIDSLKKDGTINKIMKKWLG
Q

Sequences:

>Translated_241_residues
MKFLKIFLLSCLLVSCEKKETEKTLIVGTAADNPPYEFIQDGEIVGIDIDIIKAISERLNKKVIIKNFDFNGLLAALVSE
NIDVAVAELSVTPERAEYISFSDNYATARFAIIYRTGDNIQNSKDLENKIVGVQLGSVLEKKAHDLSQTINIKVHSLANH
LMLVEELKARVIDAIISEEFQGKKLKENNSNLESSTLEEFSSDFAIGMSKNSGLINEINEAIDSLKKDGTINKIMKKWLG
Q
>Mature_241_residues
MKFLKIFLLSCLLVSCEKKETEKTLIVGTAADNPPYEFIQDGEIVGIDIDIIKAISERLNKKVIIKNFDFNGLLAALVSE
NIDVAVAELSVTPERAEYISFSDNYATARFAIIYRTGDNIQNSKDLENKIVGVQLGSVLEKKAHDLSQTINIKVHSLANH
LMLVEELKARVIDAIISEEFQGKKLKENNSNLESSTLEEFSSDFAIGMSKNSGLINEINEAIDSLKKDGTINKIMKKWLG
Q

Specific function: Part of a binding-protein-dependent transport system for arginine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=250, Percent_Identity=31.2, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788228, Length=222, Percent_Identity=27.027027027027, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787085, Length=246, Percent_Identity=26.8292682926829, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788649, Length=227, Percent_Identity=28.1938325991189, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1788648, Length=226, Percent_Identity=26.1061946902655, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787088, Length=244, Percent_Identity=24.1803278688525, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26824; Mature: 26824

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLKIFLLSCLLVSCEKKETEKTLIVGTAADNPPYEFIQDGEIVGIDIDIIKAISERLN
CHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHCCCCEEEEHHHHHHHHHHHCC
KKVIIKNFDFNGLLAALVSENIDVAVAELSVTPERAEYISFSDNYATARFAIIYRTGDNI
CEEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCHHHEEECCCCCCEEEEEEEEECCCCC
QNSKDLENKIVGVQLGSVLEKKAHDLSQTINIKVHSLANHLMLVEELKARVIDAIISEEF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGKKLKENNSNLESSTLEEFSSDFAIGMSKNSGLINEINEAIDSLKKDGTINKIMKKWLG
CCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHC
Q
C
>Mature Secondary Structure
MKFLKIFLLSCLLVSCEKKETEKTLIVGTAADNPPYEFIQDGEIVGIDIDIIKAISERLN
CHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHCCCCEEEEHHHHHHHHHHHCC
KKVIIKNFDFNGLLAALVSENIDVAVAELSVTPERAEYISFSDNYATARFAIIYRTGDNI
CEEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCHHHEEECCCCCCEEEEEEEEECCCCC
QNSKDLENKIVGVQLGSVLEKKAHDLSQTINIKVHSLANHLMLVEELKARVIDAIISEEF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGKKLKENNSNLESSTLEEFSSDFAIGMSKNSGLINEINEAIDSLKKDGTINKIMKKWLG
CCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]