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The map label for this gene is ntaB [H]

Identifier: 15892114

GI number: 15892114

Start: 198902

End: 199381

Strand: Reverse

Name: ntaB [H]

Synonym: RC0191

Alternate gene names: 15892114

Gene position: 199381-198902 (Counterclockwise)

Preceding gene: 15892115

Following gene: 15892113

Centisome position: 15.71

GC content: 29.58

Gene sequence:

>480_bases
ATGGCTGGGACAGTAACAGAAAGTCAATTTAAAGATGCTATGAGCCGATTCCCTCAGGGAGTGACTATTATAACAACAAA
TTGCAATAACGAACTTTTTGGCTTTACTGCTAGTTCTCTTACTTCCGTATCTTTAAAACCGCCGTTAATATTATTTTGCC
TTAATAAAAACTCTTTTAGTATCAACAGTTTTCAAAAAAGTGATAAGTTTGCAATTAGTATTTTAGCAGAGAATCAAATT
GATATCTCAAAACATTTTGCTAAATCACAATCTAATAAATTTACAAAAATTGATTATGAGCTTGGTAATAAAACTGATTG
CCCTTTAATAAACGGTGCAACTTATCATATAGAGTGTAATAAATATGCTTCTTACGATGCCGGTGATCATGTTATATTTA
TCGGTGAAGTAATAAATACCGCTATTAAAAATGATTTAAAGCCTCTATTATATTTTCATAAATCGTATACAAAGATTTAA

Upstream 100 bases:

>100_bases
TTGCTATAGGCATGTCTAAAAACTCAGGACTAATTAATGAAATTAATGAGGCAATCGATTCATTAAAAAAAGACGGAACC
ATTAACAAAATTATGAAAAA

Downstream 100 bases:

>100_bases
TTTTGGTTATTTTTTGCTGCAAATTATAAGATTTTTTGAAATATAAGTAACTATTCCAGCAAAAATCTTATTAATTTTCG
CTAAAAAATACCTCAAAATA

Product: hypothetical protein

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MAGTVTESQFKDAMSRFPQGVTIITTNCNNELFGFTASSLTSVSLKPPLILFCLNKNSFSINSFQKSDKFAISILAENQI
DISKHFAKSQSNKFTKIDYELGNKTDCPLINGATYHIECNKYASYDAGDHVIFIGEVINTAIKNDLKPLLYFHKSYTKI

Sequences:

>Translated_159_residues
MAGTVTESQFKDAMSRFPQGVTIITTNCNNELFGFTASSLTSVSLKPPLILFCLNKNSFSINSFQKSDKFAISILAENQI
DISKHFAKSQSNKFTKIDYELGNKTDCPLINGATYHIECNKYASYDAGDHVIFIGEVINTAIKNDLKPLLYFHKSYTKI
>Mature_158_residues
AGTVTESQFKDAMSRFPQGVTIITTNCNNELFGFTASSLTSVSLKPPLILFCLNKNSFSINSFQKSDKFAISILAENQID
ISKHFAKSQSNKFTKIDYELGNKTDCPLINGATYHIECNKYASYDAGDHVIFIGEVINTAIKNDLKPLLYFHKSYTKI

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081819, Length=156, Percent_Identity=29.4871794871795, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 17751; Mature: 17620

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTVTESQFKDAMSRFPQGVTIITTNCNNELFGFTASSLTSVSLKPPLILFCLNKNSFS
CCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCEEEEECCCCEEEEEECCCCEE
INSFQKSDKFAISILAENQIDISKHFAKSQSNKFTKIDYELGNKTDCPLINGATYHIECN
ECCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCEECCEEEEEEEC
KYASYDAGDHVIFIGEVINTAIKNDLKPLLYFHKSYTKI
CCCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AGTVTESQFKDAMSRFPQGVTIITTNCNNELFGFTASSLTSVSLKPPLILFCLNKNSFS
CCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCEEEEECCCCEEEEEECCCCEE
INSFQKSDKFAISILAENQIDISKHFAKSQSNKFTKIDYELGNKTDCPLINGATYHIECN
ECCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCEECCEEEEEEEC
KYASYDAGDHVIFIGEVINTAIKNDLKPLLYFHKSYTKI
CCCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]